Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,247,167 G→C 7.0% A24A (GCC→GCG)  RCAP_rcc01176 ← GDSL‑like lipase/acylhydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,247,1670G→C 7.0% 3.2 ‑4.0 ‑0.1131A24A (GCC→GCG) RCAP_rcc01176GDSL‑like lipase/acylhydrolase
Reads supporting (aligned to +/- strand):  new base (13/0):  ref base (82/36):  total (95/36)
Fisher's exact test strand distribution p-value = 1.92e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.16e-01

Only 100 of 216 total aligned reads displayed.

TCCGCAACCAGCCCTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTGCAGAC  >  NC_014034/1247067‑1247267
                                                                                                    |                                                                                                      
tCCGCAACCAGCCCTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCg                                                                                                        <  1:3275350/101‑1 (MQ=255)
  cGCAACCAGCCCTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCGc                                                                                                      >  2:3265777/1‑101 (MQ=255)
    cAACCAGCCCTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCGccc                                                                                                    >  1:3396762/1‑100 (MQ=255)
     aaCCAGCCCTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCGcccg                                                                                                   >  1:1376593/1‑99 (MQ=255)
     aaCCAGCCCTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCGcccc                                                                                                   >  1:2359402/1‑99 (MQ=255)
     aaCCAGCCCTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCGcccc                                                                                                   >  2:3210412/1‑99 (MQ=255)
     aaCCAGCCCTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCGCcgc                                                                                                   >  2:548225/1‑101 (MQ=255)
        cAGCCCTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGc                                                                                                >  1:3531192/1‑101 (MQ=255)
        cAGCCCCGCAGGTGCGGCTCCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGc                                                                                                <  2:2065747/101‑1 (MQ=255)
         aGCCCTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCGCCCCGGcc                                                                                               >  1:463141/1‑101 (MQ=255)
            ccTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGccccc                                                                                            >  2:2430129/1‑101 (MQ=255)
            ccTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGccccc                                                                                            >  2:2711671/1‑101 (MQ=255)
              tGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGccccccc                                                                                          <  2:2691322/101‑1 (MQ=255)
               gCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGcccccccc                                                                                         >  2:1978280/1‑100 (MQ=255)
               gCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCGCCGCGGcccccccc                                                                                         >  1:4137580/1‑100 (MQ=255)
                cAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGGCCCCccgc                                                                                        >  2:1649072/1‑101 (MQ=255)
                 aGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCCCGGCCAGAAGCGTCACCGGCTCGGCCCCGGCCCCCccccc                                                                                       >  1:1006729/1‑101 (MQ=255)
                   cTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCCGCCCCCCCCCCGa                                                                                     >  2:3706162/1‑101 (MQ=255)
                       ggCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCCCCGCTGcc                                                                                 >  1:1982628/1‑101 (MQ=255)
                        gccccAGCCCCTGTTCCGGGTCCAGCCCTTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGccc                                                                                <  2:283834/98‑1 (MQ=255)
                         cccccgCCCCTGATCCGGGTCCGGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGccaa                                                                               <  2:1078633/96‑3 (MQ=255)
                         ccccAGCCCCTGATCCGGGTCCAGCCCGTCCCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCa                                                                               <  2:4356134/99‑1 (MQ=255)
                           ccAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCCGTGccccga                                                                             >  2:4530307/1‑98 (MQ=255)
                            cAGCCCCTGATCCGGGTCCAGCCCGTCCCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAgc.g                                                                           <  2:2926673/101‑1 (MQ=255)
                            cAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCCGCCCCCCCCCCGGTGgcccacg                                                                            >  2:2207337/1‑97 (MQ=255)
                             aGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGAAGCCCAgc.g.c                                                                         >  2:2342613/1‑101 (MQ=255)
                             aGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCCCCGCTGCCCCgc.g.c                                                                         >  1:2517099/1‑101 (MQ=255)
                                cccTGATCCGGGTCCAGCCCGTCCCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCCCCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGaa                                                                      <  2:2428149/101‑1 (MQ=255)
                                cccTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCGCCGCGGCCCCCCCCCCCCGGCCCCgc.g.ccga                                                                      >  2:4172787/1‑98 (MQ=255)
                                 ccTGACCCGGGTCCAGCCCGTCCCCCTGGGTCAGGCGGTCGCCCACGGCCAGAAGCGTCCCCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGaaa                                                                     <  1:1482772/101‑1 (MQ=255)
                                  cTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCCCCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGaaaa                                                                    <  1:553170/101‑1 (MQ=255)
                                   tGATCCGGGTCCAGCCCGTCCCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCCCCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAAt                                                                   <  1:1192603/101‑1 (MQ=255)
                                   tGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCGCCGCGGCCCCCCCCCCGGCGCCCAGc.g.cggcaat                                                                   >  2:4449016/1‑96 (MQ=255)
                                    gATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATc                                                                  >  1:3103498/1‑101 (MQ=255)
                                    gATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCGCCGCGGCCCCCCCCCCGATGCCCAGC.G.CGGAAATc                                                                  >  2:2251601/1‑101 (MQ=255)
                                    gATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCGCCGCCGCCCCCCCCCCCATCCCCCGCGG.CGaaaac                                                                   >  1:4175389/1‑100 (MQ=255)
                                     aTCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCCCCCCTGCCCCGC.G.CGAAAAAcc                                                                 >  1:3990806/1‑101 (MQ=255)
                                        cGGGTCCAGCCCGTCCCCCTGGGTCAGGCTGCCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCg                                                              <  2:685441/101‑1 (MQ=255)
                                          ggTCCGGCCCGCCCCCCGGGGTCCGGCTGTCGCCCAGGGCCAGAAGCGTCCCGGGCTCGCCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCa                                                            <  2:1472098/101‑1 (MQ=255)
                                          ggTCCAGCCCGTCCCCCTGCGCCAGGCTGTCGCCCACGGCCAGAAGCCTCGCCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCa                                                            <  2:1407700/101‑1 (MQ=255)
                                           gTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCCCCGGTGCCCCCC.G.CGGAAAAccccccca                                                           >  2:3247370/1‑97 (MQ=255)
                                           gTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCCCCGATGCCCCGC.G.CGAAAATCCCCGCaa                                                           >  2:2989323/1‑101 (MQ=255)
                                            tCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCCCCCATGCCCCGC.G.CGAAAAACCCCGCaac                                                          >  2:2404809/1‑100 (MQ=255)
                                            cccAGCCCGTCCCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCCCCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAg                                                          <  2:852817/100‑1 (MQ=255)
                                             ccAGCCCGTCCCCCTGGGTCAGGCTGCCGCCCACGGCCAGAAGCGTCCCCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAgc                                                         <  1:683515/101‑1 (MQ=255)
                                             ccAGCCCGTACCCCTGGGTCAGGCTGTCGCCCCCGGCCAGAAACGTCACCGGCTCCGCCGCGGGCCCCCCCCCGTTGCCCCGC.G.CGAAAATCCCCCCCAgc                                                         >  1:3727939/1‑101 (MQ=255)
                                             ccAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAgc                                                         >  2:379771/1‑101 (MQ=255)
                                               aGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCGCCGCGGCCCCCCCGCCGATGCCCCAC.G.CCAAAAACCCCCCAAgcgc                                                       >  2:2268996/1‑101 (MQ=255)
                                                 cccGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCGCCGCGGGCCCCCCCCCGAGGCCCAGC.G.CGAAAACCCCCGCAAGcaccg                                                     >  1:224004/1‑101 (MQ=255)
                                                  ccGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGCTGCCCCGC.G.CGAAAATCCCCCCCAGcgccgc                                                    >  2:1924947/1‑101 (MQ=255)
                                                     tACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCCCAAGCGCCGCCtt                                                 >  1:4226862/1‑101 (MQ=255)
                                                     tACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCCCCGATGCCCCGC.G.CGCAAAACCCCGCAAGCGCCCCCtt                                                 >  2:1269379/1‑101 (MQ=255)
                                                      aCCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCCCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAAACCCCCCAAGCGCCGCCCTg                                                >  1:2163135/1‑101 (MQ=255)
                                                      aCCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCGCCGCGGGCCCCCAGCCGAGGCCCGGG.G.CGAAAATCCCCGCAAGCCCCCCCTTg                                                >  1:1552358/1‑101 (MQ=255)
                                                       ccccTGGGTCAGGCTGTCGCCCACGGCCAGAGGCGCCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGc                                               <  2:2548669/101‑1 (MQ=255)
                                                       ccccTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCCCTGCCCCGC.G.CGCAAAACCCCCCCCGCgccgccctcc                                               >  2:3396449/1‑97 (MQ=255)
                                                        cccTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGcc                                              >  1:545260/1‑101 (MQ=255)
                                                        cccTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATCCCCAGG.C.CGAAAAGCCCTCAAAGCGCGCCCTTGcc                                              >  2:3195112/1‑101 (MQ=255)
                                                         ccTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCCGC.G.CGAAAATACCCGCAAACGCCGCCTTGccc                                             >  2:2659443/1‑101 (MQ=255)
                                                          ctggagcAGGCTGCCGCCCACGGCCAGAAGCGTCCCCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCa                                            <  2:211653/95‑1 (MQ=255)
                                                          cTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCCCCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCa                                            <  1:1275455/101‑1 (MQ=255)
                                                           tGGGTCAGGCTGTCGCCCCCGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGCTGCCCACC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCAg                                           >  2:1563864/1‑101 (MQ=255)
                                                           tGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCCCCCATGCCCACC.G.CGAAAAACCCCCCAAGCGCCGCCTTTCCCac                                           >  2:3550547/1‑100 (MQ=255)
                                                           tGGGTCAGGCTGCCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGCCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCAg                                           <  1:468436/101‑1 (MQ=255)
                                                               tCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCGCCGCGGCCCCCCCGCTGATGCCCCGC.G.CGAAAATCCCCCCAAGCGCCGCCTTGCCCAgccgc                                       >  1:1884900/1‑101 (MQ=255)
                                                                cAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGcagatcggaa                                      >  1:1469404/1‑92 (MQ=255)
                                                                cAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCCCGGCCCCCCCTCCGGGGCCCTGC.G.CGGAGATCCCCGCAACCTCCTCCTTGCCCAgccgcc                                      >  1:3931720/1‑101 (MQ=255)
                                                                 aGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCGCCGCGGCCCCCCCGCCGATGCCCCGC.G.CGGAAAACCCCGCAAACGCCCCCTTGCCCCgccgccc                                     >  2:1757774/1‑100 (MQ=255)
                                                                 aGGCGGTCCCCCCGGGCCAGAGGCGTCCCCGGCGCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCAgccgccg                                     <  1:3809736/101‑1 (MQ=255)
                                                                  ggCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCCCCCCTTCCCCGC.G.CGAAAAAACCCCCAAGCGCCGCCTTGCCCCgccccccc                                    >  1:4180283/1‑99 (MQ=255)
                                                                   gCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCCAGCGCCGCCTTGCCCAgccgccgcc                                   >  1:1716352/1‑101 (MQ=255)
                                                                   gCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCCCCCGCGCCCCAC.GCCGAAAACCCCCCCAACCCCCCCCTTCccccccgccccc                                    >  2:4117813/1‑92 (MQ=255)
                                                                    cTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCCCCGATGCCCCGC.G.CGAAAATCCCCCCAAGCGCCGCCCTGCCCCgccgccgccc                                  >  1:1343067/1‑100 (MQ=255)
                                                                     tGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCCGCcgccgccgc                                 >  1:2800727/1‑101 (MQ=255)
                                                                         gCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAAACCCCGCAAGCGCCGCCTTGCCCAgccgcccgcgccacc                             >  2:1157104/1‑92 (MQ=255)
                                                                           ccACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCCCACCCCa                           >  1:3221284/1‑101 (MQ=255)
                                                                                 ccAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCCCCCCCCCTATCCg                     >  2:516198/1‑101 (MQ=255)
                                                                                   aGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGt                   >  1:250126/1‑101 (MQ=255)
                                                                                   aGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAAACCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCCTATCCGGt                   >  1:4440616/1‑101 (MQ=255)
                                                                                    gaggCGTCCCCGCCCCGGCGGCGCCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCACCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGtt                  <  2:4246795/98‑1 (MQ=255)
                                                                                    aagccgcccccGGCCCGGCCGCGCCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGtt                  <  2:1631856/92‑1 (MQ=255)
                                                                                     aaGCGCCCCCGGCCCGCCCGCGCCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTg                 <  1:1785611/101‑1 (MQ=255)
                                                                                        cGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATc              >  2:4690070/1‑101 (MQ=255)
                                                                                         gTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATcc             >  2:3975873/1‑101 (MQ=255)
                                                                                         gTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATcc             >  2:3915508/1‑101 (MQ=255)
                                                                                         gTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CCAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCACATCCGGTTGATcc             >  1:1151151/1‑101 (MQ=255)
                                                                                           cccgggCCCGCCCGCGCCCCCCCCGCCGATGCCCAGC.G.CGAAAACCCCCGCAAGCGCGGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATcccc           <  2:1552257/97‑1 (MQ=255)
                                                                                            aCCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCCGCCGCCGCCGCACCCCATATCCGGTTGATCCCCt          >  2:1051549/1‑101 (MQ=255)
                                                                                            aCCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCCCCTTGCCCAGCCCCCGCCGCACCCCATATCCGGTTGATCCCCt          >  1:3060415/1‑101 (MQ=255)
                                                                                             ccGGCTCGGCCGCGCCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCtt         <  2:2516954/101‑1 (MQ=255)
                                                                                              cGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCttt        >  1:4515400/1‑101 (MQ=255)
                                                                                              cGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCttt        >  2:732842/1‑101 (MQ=255)
                                                                                               ggCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCCCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTg       >  1:815874/1‑101 (MQ=255)
                                                                                                gcccGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTGc      <  1:3881766/98‑1 (MQ=255)
                                                                                                 cTCGCCCGCGCCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTGCa     <  1:2676475/101‑1 (MQ=255)
                                                                                                  tCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTGCAg    >  1:1312908/1‑101 (MQ=255)
                                                                                                  tCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTGCAg    >  2:4565138/1‑101 (MQ=255)
                                                                                                  ccGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTGCAg    <  2:3456438/100‑1 (MQ=255)
                                                                                                   cGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTGCAGa   >  2:3294899/1‑101 (MQ=255)
                                                                                                    ggCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTGCAGAc  >  1:581000/1‑101 (MQ=255)
                                                                                                    |                                                                                                      
TCCGCAACCAGCCCTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGC.G.CGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTGCAGAC  >  NC_014034/1247067‑1247267

Base quality scores:  ATCG  < 3 ≤  ATCG  < 8 ≤  ATCG  < 20 ≤  ATCG  < 31 ≤  ATCG  < 41 ≤  ATCG