Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,325,125 G→T 100% P351T (CCC→ACC)  dacC2 ← D‑alanyl‑D‑alanine carboxypeptidase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,325,1250G→T100.0% 676.4 185P351T (CCC→ACC) dacC2D‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  new base (94/91):  ref base (0/0):  total (94/91)

Only 100 of 216 total aligned reads displayed.

ACCATCTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGGGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCTCGGCC  >  NC_014034/1325025‑1325222
                                                                                                    |                                                                                                 
accaTCTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACggg                                                                                                   >  2:4488671/1‑101 (MQ=255)
  caTCTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGGAGCCGCCCTCGGCCACGTAGGTTTCCGCCACCAGCGGCACGGGgc                                                                                                 >  1:3989917/1‑101 (MQ=255)
  caTCTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTgc                                                                                                 <  2:4093482/101‑1 (MQ=255)
   aTCTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTgcg                                                                                                >  1:1027450/1‑101 (MQ=255)
      tGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcac                                                                                             >  1:320620/1‑100 (MQ=255)
       gACGGGTCTGCGCCAGAAGCCGGCCGGCGGCGGCGGTGAAGCGGCCCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagc                                                                                            <  2:4696561/101‑1 (MQ=255)
       gACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCCGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagc                                                                                            <  2:4410215/101‑1 (MQ=255)
        aCGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTTCGCCACCAGCGGCACGGTGCGcagcc                                                                                           >  2:4157679/1‑101 (MQ=255)
        aCGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagcc                                                                                           <  1:1921234/101‑1 (MQ=255)
         cGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagccc                                                                                          >  2:2102003/1‑101 (MQ=255)
         cGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagccc                                                                                          <  1:563938/101‑1 (MQ=255)
          gggTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagcccc                                                                                         <  1:1314640/101‑1 (MQ=255)
            gTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagccccag                                                                                       <  2:1766480/101‑1 (MQ=255)
              cTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagccccagcc                                                                                     <  1:3913274/101‑1 (MQ=255)
                gctccAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCagccccagcccc                                                                                   <  2:3999363/98‑1 (MQ=255)
                gcgcCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCagccccagcccc                                                                                   <  1:1564285/101‑1 (MQ=255)
                 cgcCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCg                                                                                  <  1:1960231/101‑1 (MQ=255)
                  gcCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGTAGCCCCAGCCCCgg                                                                                 <  2:1200519/101‑1 (MQ=255)
                   ccAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGc                                                                                >  1:813784/1‑101 (MQ=255)
                   ccAGAACCCGGCCCCCGGGGGCGGCGAAGGGGACCCGGAAGCCGCCCTCGGCCACGCCGGTTTCCGGCACAAGCGGCACGGTGCGCAGCCCCAGCCCCGGc                                                                                <  2:1334376/101‑1 (MQ=255)
                    cAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCa                                                                               >  2:4352912/1‑101 (MQ=255)
                     aGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCAc                                                                              <  1:602895/101‑1 (MQ=255)
                      gAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACg                                                                             >  2:463000/1‑101 (MQ=255)
                      gAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACg                                                                             <  1:1217203/101‑1 (MQ=255)
                       aaGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACgg                                                                            >  1:4249400/1‑101 (MQ=255)
                       aaGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACgg                                                                            >  2:672101/1‑101 (MQ=255)
                        aGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCTACGTCGGTTTCCGCCACCAGCGGTACGGTGCGCAGACCCAGCCCCGGCACTGt                                                                           >  2:796769/1‑101 (MQ=255)
                        aGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACggg                                                                           >  1:2066339/1‑100 (MQ=255)
                        aGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGt                                                                           >  1:2282000/1‑101 (MQ=255)
                           cGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCGCGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGAt                                                                        <  1:2474342/101‑1 (MQ=255)
                           cGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGAt                                                                        <  2:237804/101‑1 (MQ=255)
                           cGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGAt                                                                        <  2:4284940/101‑1 (MQ=255)
                            ggCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGTACGGTGCGCAGCCCCAGCCCCGGCACGGTGAtt                                                                       >  1:4118488/1‑101 (MQ=255)
                             gCCCGCGGCGGCGGTGAAGCGGACCAGGAGGCCGCCCTCGGCCACGTCGGTTTCGGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGAttt                                                                      <  2:190691/101‑1 (MQ=255)
                             gCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGAttt                                                                      <  1:297903/101‑1 (MQ=255)
                              cccGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTc                                                                     <  1:2323357/101‑1 (MQ=255)
                               ccGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTcc                                                                    <  2:3671163/101‑1 (MQ=255)
                               ccGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTcc                                                                    <  1:1016887/101‑1 (MQ=255)
                               ccGCGGCGGCAGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTcc                                                                    <  1:3734388/101‑1 (MQ=255)
                                cgcgGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCa                                                                   >  1:3556297/1‑101 (MQ=255)
                                cgcgGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCa                                                                   <  2:3830661/101‑1 (MQ=255)
                                cgcgGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCa                                                                   <  1:2747016/101‑1 (MQ=255)
                                 gcggcggcgGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAg                                                                  <  1:2240690/101‑1 (MQ=255)
                                 gcggcggcgGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAg                                                                  <  2:4515062/101‑1 (MQ=255)
                                  cggcggcggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAgc                                                                 >  1:1479758/1‑101 (MQ=255)
                                    gcggcggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAgccg                                                               >  1:3413959/1‑101 (MQ=255)
                                        cggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGcccc                                                           <  2:3072120/101‑1 (MQ=255)
                                         ggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCa                                                          >  1:149702/1‑101 (MQ=255)
                                         ggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCa                                                          <  1:2871695/101‑1 (MQ=255)
                                         ggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATCTCCAGCCGCCCCa                                                          >  1:2827659/1‑101 (MQ=255)
                                          gTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAg                                                         <  2:3515723/101‑1 (MQ=255)
                                            gAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCt                                                       <  2:1146877/101‑1 (MQ=255)
                                            gAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCt                                                       >  1:2907656/1‑101 (MQ=255)
                                            gAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCACCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCt                                                       >  1:3594844/1‑101 (MQ=255)
                                             aaGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTc                                                      >  1:1234826/1‑101 (MQ=255)
                                               gCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCgg                                                    >  1:2190516/1‑101 (MQ=255)
                                                cGGACCCGGAAGCCCCCCCCGGCCACTTCGGTTTCCGCCACCAGCGGCCCGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGt                                                   <  1:2503462/101‑1 (MQ=255)
                                                 ggACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTg                                                  <  2:3556078/101‑1 (MQ=255)
                                                   aCCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGcc                                                >  2:2408317/1‑101 (MQ=255)
                                                     cAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCccg                                              >  2:3888230/1‑101 (MQ=255)
                                                     cAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCccg                                              >  2:2460295/1‑101 (MQ=255)
                                                       ggAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCccgcc                                            >  2:3263825/1‑101 (MQ=255)
                                                       ggAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCccgcc                                            <  1:4329586/101‑1 (MQ=255)
                                                         aacccgccCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGt                                          <  1:518853/98‑1 (MQ=255)
                                                         aaGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGt                                          >  1:4610228/1‑101 (MQ=255)
                                                         aaGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGCGATTTCCAGCCGCCCCAGCTCGGTGCccgccgc                                          >  2:3173883/1‑100 (MQ=255)
                                                          aGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTc                                         <  1:3189136/101‑1 (MQ=255)
                                                                ccTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGgc                                   >  2:127029/1‑101 (MQ=255)
                                                                 cTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGgcg                                  <  2:523623/101‑1 (MQ=255)
                                                                   cagCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGcgcg                                >  2:2221054/3‑101 (MQ=255)
                                                                   cGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGcgcg                                >  1:949053/1‑101 (MQ=255)
                                                                     gCCCCGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGACGCGGt                              <  2:620035/101‑1 (MQ=255)
                                                                      ccACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTg                             <  2:1060042/101‑1 (MQ=255)
                                                                       cACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGa                            >  1:4144621/1‑101 (MQ=255)
                                                                           tCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCgg                        <  1:1382291/101‑1 (MQ=255)
                                                                             ggTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGc                      >  1:107348/1‑101 (MQ=255)
                                                                             ggTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGc                      >  1:888405/1‑101 (MQ=255)
                                                                              gTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGcc                     <  1:3791248/101‑1 (MQ=255)
                                                                              gTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGcc                     >  2:1579493/1‑101 (MQ=255)
                                                                               tttCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCg                    >  1:4357234/1‑101 (MQ=255)
                                                                                tcccGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGt                   <  2:1358319/99‑1 (MQ=255)
                                                                                 tCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTc                  >  2:284928/1‑101 (MQ=255)
                                                                                 tCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTc                  >  1:4711692/1‑101 (MQ=255)
                                                                                 tCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTc                  >  1:1654684/1‑101 (MQ=255)
                                                                                  ccGCCACCAGCGGAAGGGTGCCCAGCCCCAGCCCGGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCa                 <  2:3413753/101‑1 (MQ=255)
                                                                                   ccccaccaGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCaa                <  1:4488921/99‑1 (MQ=255)
                                                                                    gCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCaaa               <  2:88566/101‑1 (MQ=255)
                                                                                     ccaccaGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAg              >  2:4455191/1‑101 (MQ=255)
                                                                                       accaGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGAc            >  2:1828504/1‑101 (MQ=255)
                                                                                        ccaGCGCCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGAcc           <  1:1923571/101‑1 (MQ=255)
                                                                                         caGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCg          >  1:2395055/1‑101 (MQ=255)
                                                                                             ggCCCGGGGCCCAGCCCCAGCCCCGGCGCGGTGATTTCCAGCCGCCCCAGCTCGGGGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCTc      <  2:739277/101‑1 (MQ=255)
                                                                                             ggCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCTc      <  2:2281867/101‑1 (MQ=255)
                                                                                              gCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCTCg     >  1:1344425/1‑101 (MQ=255)
                                                                                              gCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCTCg     >  1:2111472/1‑101 (MQ=255)
                                                                                                aCGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCTCGGc   >  2:3966590/1‑101 (MQ=255)
                                                                                                 cGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCTCGGcc  >  2:216542/1‑101 (MQ=255)
                                                                                                 cGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCTCGGcc  >  2:273688/1‑101 (MQ=255)
                                                                                                 cGGTGCGCAGCCCCAGCCCCGGCACGGGGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCTCGGcc  <  2:2190392/101‑1 (MQ=255)
                                                                                                 cGGGGCCCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGACTCGGcc  <  1:169042/101‑1 (MQ=255)
                                                                                                    |                                                                                                 
ACCATCTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGGGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCTCGGCC  >  NC_014034/1325025‑1325222

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG