Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,388,183 A→T 100% M246F (ATC→TTC)  RCAP_rcc01300 → Cas3 family CRISPR‑associated helicase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,388,1830A→T100.0% 848.1 221M246F (ATC→TTC) RCAP_rcc01300Cas3 family CRISPR‑associated helicase
Reads supporting (aligned to +/- strand):  new base (111/110):  ref base (0/0):  total (111/110)

Only 100 of 231 total aligned reads displayed.

CGCATATGGCCGCCTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGATCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATCGGCGGATCATCTA  >  NC_014034/1388085‑1388280
                                                                                                  |                                                                                                 
cgcATATGGCCGCCTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTc                                                                                                 <  2:2803431/101‑1 (MQ=255)
cgcATATGGCCGCCTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTc                                                                                                 <  1:1818368/101‑1 (MQ=255)
 gcATATGGCCGCCTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCa                                                                                                <  1:1786902/101‑1 (MQ=255)
  cATATGGCCGCCTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCAc                                                                                               <  1:781036/101‑1 (MQ=255)
  cATATGGCCGCCTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCAc                                                                                               <  2:4077799/101‑1 (MQ=255)
  cATATGGCCGCCTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCAc                                                                                               >  1:2539977/1‑101 (MQ=255)
  cATATGGCCGCCTTTGACCCCGGAACCGAGCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCGCGGCCGCGGCTGCACTGCCGCCGGGCCTGGTCAc                                                                                               >  2:1110868/1‑101 (MQ=255)
     atGGCCGCCTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCct                                                                                            <  2:4574022/101‑1 (MQ=255)
      tGGCCGCCTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCctc                                                                                           <  2:1552553/101‑1 (MQ=255)
         ccgccTTTGCCCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCg                                                                                        <  2:553977/101‑1 (MQ=255)
         ccgccTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCg                                                                                        <  2:2226982/101‑1 (MQ=255)
         ccgccTTTGACCCCCGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCg                                                                                        <  2:2367916/101‑1 (MQ=255)
           gccTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGt                                                                                      <  2:719477/101‑1 (MQ=255)
              tttGACCCCGGAAGCGATCTGAGCCGCCTGCGCGCCGAAATCCTGGCGAAGGTCCGGGCCAAGGCTGCACTGCCGCCGGGCCTGTTCCCCCTCTCGGTccc                                                                                   <  2:3144921/101‑1 (MQ=255)
              tttGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTccc                                                                                   >  2:3282236/1‑101 (MQ=255)
               ttGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCg                                                                                  >  1:414924/1‑101 (MQ=255)
                tGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGa                                                                                 >  2:3753762/1‑101 (MQ=255)
                tGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGa                                                                                 <  1:2820066/101‑1 (MQ=255)
                 gcccccGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGAc                                                                                <  2:3111319/99‑1 (MQ=255)
                 gACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGAc                                                                                <  1:4592768/101‑1 (MQ=255)
                 gACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGAc                                                                                >  1:4349465/1‑101 (MQ=255)
                 gACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGAc                                                                                <  2:3538097/101‑1 (MQ=255)
                 gACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGAc                                                                                >  2:4220985/1‑101 (MQ=255)
                 gACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGAc                                                                                <  2:667885/101‑1 (MQ=255)
                 gACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGAc                                                                                >  1:1153929/1‑101 (MQ=255)
                    cccGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACggg                                                                             >  1:1745318/1‑101 (MQ=255)
                    cccGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACggg                                                                             >  2:78627/1‑101 (MQ=255)
                     ccGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGc                                                                            <  2:1706894/101‑1 (MQ=255)
                      cGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCg                                                                           >  1:406642/1‑101 (MQ=255)
                       ggAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGGAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTTACCCTCTCGGTGCCGACggggag                                                                          >  2:4728041/1‑98 (MQ=255)
                        gAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCggg                                                                         >  2:2060825/1‑100 (MQ=255)
                        gAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGGCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTGACCCTCTCGGTCCCGACGGGCGGt                                                                         >  2:4438892/1‑101 (MQ=255)
                         aaCCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTg                                                                        >  1:2828090/1‑101 (MQ=255)
                         aaCCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTg                                                                        >  1:1941944/1‑101 (MQ=255)
                          aCCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTgg                                                                       <  2:3629036/101‑1 (MQ=255)
                          aCCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTgg                                                                       <  2:1974745/101‑1 (MQ=255)
                          aCCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTgg                                                                       <  2:1402217/101‑1 (MQ=255)
                          aCCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTgg                                                                       >  1:2080468/1‑101 (MQ=255)
                            cGATCTGAACTGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGaa                                                                     <  2:2294717/101‑1 (MQ=255)
                               tCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGaaaaa                                                                  >  1:2452087/1‑101 (MQ=255)
                                 tGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAAcc                                                                <  1:4426734/101‑1 (MQ=255)
                                 tGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAAcc                                                                >  2:2724697/1‑101 (MQ=255)
                                 tGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAAcc                                                                <  1:3770254/101‑1 (MQ=255)
                                   aaCCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCt                                                              <  1:3980780/101‑1 (MQ=255)
                                   aaCCGCCTGCGCGCCGAAATCCTGGCCCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCt                                                              <  1:717684/101‑1 (MQ=255)
                                     ccGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTg                                                            <  2:4303772/101‑1 (MQ=255)
                                       gCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGcc                                                          >  1:4285864/1‑101 (MQ=255)
                                        ccTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCt                                                         <  2:883326/101‑1 (MQ=255)
                                          tGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCg                                                       <  2:4232717/101‑1 (MQ=255)
                                           gcgcgcCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGc                                                      <  2:2646573/101‑1 (MQ=255)
                                             gcgcCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCtt                                                    <  1:3355841/101‑1 (MQ=255)
                                             gcgcCGAAAGCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCtt                                                    <  1:3667273/101‑1 (MQ=255)
                                               gcCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTgg                                                  <  2:1241753/101‑1 (MQ=255)
                                               gcCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTgg                                                  >  2:869578/1‑101 (MQ=255)
                                                  gAAGTCCTGGGGCAGGTCCGGGCCAAGGCCCGTGGGCCGCCGGGCCTGTTCCCCCTCTCGGTCCCCACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCtt                                               <  1:123946/101‑1 (MQ=255)
                                                  gAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCtt                                               >  1:3641128/1‑101 (MQ=255)
                                                   actcctgggCGCAGCTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCttt                                              <  1:3420403/94‑1 (MQ=255)
                                                   aaaTCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCttt                                              <  1:3875137/101‑1 (MQ=255)
                                                     aTCCTGGCGCAGTTCCGGGCCAAGGCCCCCGTGCCGCCGCGCCTGTTCGCCCTCTCGGACCCGACGGGCGGTGGAAAACCCCTTGCCTCGCTTGGGTTTgc                                            <  2:3515068/101‑1 (MQ=255)
                                                      tCCTGCCGCGGGTCCGGGCCAAGGCCGCACTGCCGCCAGGCCTGTTCACCCTCTCGGCCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTgcg                                           <  2:441676/101‑1 (MQ=255)
                                                        cTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCt                                         >  2:3714862/1‑101 (MQ=255)
                                                         tGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTg                                        <  2:3400319/101‑1 (MQ=255)
                                                         tGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTg                                        >  1:2216566/1‑101 (MQ=255)
                                                           gcgcAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCGCCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGa                                      <  2:317662/101‑1 (MQ=255)
                                                           gcgcAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGa                                      >  1:4693125/1‑101 (MQ=255)
                                                            cgcAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGAt                                     <  1:2626208/101‑1 (MQ=255)
                                                             gcAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATc                                    <  2:391949/101‑1 (MQ=255)
                                                             gcAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATc                                    <  2:3998069/101‑1 (MQ=255)
                                                             gcAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATc                                    <  2:956109/101‑1 (MQ=255)
                                                             gcAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATc                                    >  1:3258015/1‑101 (MQ=255)
                                                             gcAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATc                                    <  1:719130/101‑1 (MQ=255)
                                                              cAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCa                                   <  1:2158196/101‑1 (MQ=255)
                                                               gggTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCAc                                  <  2:2037590/100‑1 (MQ=255)
                                                               aGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCAc                                  >  1:1473146/1‑101 (MQ=255)
                                                                   ccGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCCCCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACgcgg                              <  2:1426683/101‑1 (MQ=255)
                                                                   ccGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCCCCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACgcgg                              <  1:258389/101‑1 (MQ=255)
                                                                    cGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACgcggc                             <  1:3522671/101‑1 (MQ=255)
                                                                    cGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACgcggc                             >  1:2874024/1‑101 (MQ=255)
                                                                    cGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACgcggc                             >  2:3430262/1‑101 (MQ=255)
                                                                     gggCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACgcggcg                            >  1:1299963/1‑101 (MQ=255)
                                                                     gggCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACgcggcg                            >  1:3081552/1‑101 (MQ=255)
                                                                      ggCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGt                           <  2:414880/101‑1 (MQ=255)
                                                                       gCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTg                          <  1:4231996/101‑1 (MQ=255)
                                                                          aaGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGG.AAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGGGGGCCAc                      >  2:3898993/1‑101 (MQ=255)
                                                                           aGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCAc                      >  1:54233/1‑101 (MQ=255)
                                                                           aGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCAc                      <  2:1850466/101‑1 (MQ=255)
                                                                             gCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACgg                    >  1:4458068/1‑101 (MQ=255)
                                                                               cGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGc                  >  1:2991819/1‑101 (MQ=255)
                                                                                gCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCa                 >  1:1366808/1‑101 (MQ=255)
                                                                                   cTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATcg              <  2:2597048/101‑1 (MQ=255)
                                                                                   cTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATcg              <  1:4537640/101‑1 (MQ=255)
                                                                                     gccgccGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATcggc            >  1:994226/1‑101 (MQ=255)
                                                                                      ccgccgGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATcggcg           >  2:38604/1‑101 (MQ=255)
                                                                                       cgccgGGCCTTTTTCCCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATcggcgg          <  1:876014/101‑1 (MQ=255)
                                                                                        gccgGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATCGGCGGa         <  1:1718932/101‑1 (MQ=255)
                                                                                        gccgGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATCGGCGGa         >  2:258689/1‑101 (MQ=255)
                                                                                        gccgGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATCGGCGGa         <  2:283549/101‑1 (MQ=255)
                                                                                           gggCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATCGGCGGatca      <  2:1816209/101‑1 (MQ=255)
                                                                                             gCCTGTTCACCCTCTCGGTCTCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATCGGCGGatcatc    >  2:332168/1‑101 (MQ=255)
                                                                                             gCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATCGGCGGatcatc    <  1:3736888/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CGCATATGGCCGCCTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGATCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATCGGCGGATCATCTA  >  NC_014034/1388085‑1388280

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG