Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,411 C→G 4.6% intergenic (+223/‑708) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,4110C→G 4.6% 17.6 ‑1.5 ‑0.2240intergenic (+223/‑708)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/7):  ref base (130/99):  total (134/106)
Fisher's exact test strand distribution p-value = 2.22e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.78e-01

Only 100 of 255 total aligned reads displayed.

CTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCC  >  NC_014034/1395311‑1395511
                                                                                                     |                                                                                                     
 ttCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCt                                                                                                      <  2:3084561/101‑1 (MQ=255)
  tCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTg                                                                                                     <  2:397111/101‑1 (MQ=255)
   cGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGc                                                                                                    <  1:2102787/101‑1 (MQ=255)
    gCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGcc                                                                                                   <  2:2145914/101‑1 (MQ=255)
    gCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGcc                                                                                                   >  2:261299/1‑101 (MQ=255)
     caccacCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcg                                                                                                  >  1:2689660/1‑101 (MQ=255)
     caccacCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcg                                                                                                  >  2:4162668/1‑101 (MQ=255)
      accaccGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCCGCcgg                                                                                                 >  1:1672629/1‑101 (MQ=255)
       ccaccGGAGCAATTTACACCGTCTGGTCAGCAGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggt                                                                                                <  1:1523475/101‑1 (MQ=255)
        caccGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtg                                                                                               <  2:1737341/101‑1 (MQ=255)
        caccGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcgggg                                                                                               >  1:1534327/1‑99 (MQ=255)
           cGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatc                                                                                            >  2:3417267/1‑101 (MQ=255)
           cGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGAACGAAACTGCACCCGCTGGCCCTGCccgtcatc                                                                                            >  2:1249249/1‑101 (MQ=255)
               gCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcggtg                                                                                        <  1:3023934/101‑1 (MQ=255)
                cAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcggtg.a                                                                                      >  2:4648326/1‑101 (MQ=255)
                aaaTTTACACCGTCAGGATAGCGGGTTAGGCAACGGCAGGCG.CACCCCCCGCGGGGGCTTGAATCGAATCCGCACCCGCTGGCCCTGCcggtgatcggtg.a                                                                                      <  2:4479599/100‑1 (MQ=255)
                 aaTTTACCCCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCAAAACTGCACCCGCTGGCCCTGCcggtgatcggtg.at                                                                                     <  1:4025066/101‑1 (MQ=255)
                  aTTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCggtgatcggtg.atc                                                                                    <  1:2394836/101‑1 (MQ=255)
                  aTTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCggtgatcggtg.atc                                                                                    <  2:1541292/101‑1 (MQ=255)
                  aTTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCggggatcggtg.atc                                                                                    >  1:902124/1‑101 (MQ=255)
                  aTTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCggggatcgggg.atc                                                                                    >  2:851077/1‑101 (MQ=255)
                    tttccccGGCTGGTCCGGGGGTTTCGGAACGGGAGTCGCCCCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCa                                                                                   <  1:122525/96‑1 (MQ=25)
                    ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                                  >  1:1576605/1‑101 (MQ=255)
                    ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                                  >  1:3446605/1‑101 (MQ=255)
                      acacCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCg                                                                                >  1:2781062/1‑101 (MQ=255)
                      acacCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGGG.ATCAGCg                                                                                >  2:2692648/1‑101 (MQ=255)
                         ccGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTc                                                                             >  1:805163/1‑101 (MQ=255)
                           gTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGc                                                                           <  2:901034/101‑1 (MQ=255)
                           gTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGGGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGc                                                                           >  1:1128238/1‑101 (MQ=255)
                            tCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCt                                                                          <  1:1888895/101‑1 (MQ=255)
                             cTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTc                                                                         <  2:2509369/101‑1 (MQ=255)
                              tGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTcc                                                                        >  1:1258899/1‑101 (MQ=255)
                              tGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTcc                                                                        >  2:241564/1‑101 (MQ=255)
                              tGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTcc                                                                        >  1:3276057/1‑101 (MQ=255)
                                 tCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTccccc                                                                     <  1:1565066/101‑1 (MQ=255)
                                  cAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCGcccccc                                                                    >  1:768114/1‑101 (MQ=255)
                                   aGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCcg                                                                   >  1:3334749/1‑101 (MQ=255)
                                   aGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCcg                                                                   >  1:4362631/1‑101 (MQ=255)
                                        gTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCgggg                                                              <  2:3851517/101‑1 (MQ=255)
                                        gTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCgggg                                                              >  2:361635/1‑101 (MQ=255)
                                         ttACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCCGCCGGTGATCGGTG.ATCAGCGGGCGCTCCCCCCGCggggg                                                             >  1:1972874/1‑101 (MQ=255)
                                          tACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGc                                                            <  1:1773351/101‑1 (MQ=255)
                                          tACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGGG.ATCAGCGGTCGCTCCCCCCGCGCGGGc                                                            >  2:823335/1‑101 (MQ=255)
                                           aCGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCg                                                           >  1:2835863/1‑101 (MQ=255)
                                             gAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTg                                                         <  1:3805763/101‑1 (MQ=255)
                                             caaccGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTg                                                         <  2:2555237/97‑1 (MQ=255)
                                              aaaCGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTgg                                                        <  2:2934059/101‑1 (MQ=255)
                                              aaaCGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGGGATCGGGG.ATCAGCGGGCGCTCCCCCCGCGGGGGCGTgg                                                        >  1:1392815/1‑101 (MQ=255)
                                                 cGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                     <  2:4131801/101‑1 (MQ=255)
                                                 acactgTCG.CTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                   >  2:2395214/6‑101 (MQ=2)
                                                  cactgTCG.CTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                  <  1:550018/97‑1 (MQ=2)
                                                     tgTCG.CTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                               <  2:364363/100‑2 (MQ=2)
                                                     tgTCG.CTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                               <  2:4389553/100‑2 (MQ=2)
                                                     aGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGGGGATCGaaa                                                 >  2:377209/1‑101 (MQ=255)
                                                     aGTCG.CCCCCCCCGCGGGGGCGTGGATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 <  1:403347/101‑1 (MQ=255)
                                                      gTCG.CTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                              <  1:3162291/101‑3 (MQ=2)
                                                        cG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                              >  1:2278449/1‑101 (MQ=255)
                                                        cG.CCCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                              <  1:3567299/101‑1 (MQ=255)
                                                         g.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCGGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                             >  1:4719794/1‑101 (MQ=255)
                                                            tCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgc                                           >  1:1799584/1‑101 (MQ=255)
                                                             ccccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACATcgcc                                          >  2:1314802/1‑101 (MQ=255)
                                                               ccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgccgc                                        <  1:1042531/101‑1 (MQ=255)
                                                                  cgcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCttt                                     <  1:4162914/101‑1 (MQ=255)
                                                                     gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGc                                  >  2:2162733/1‑101 (MQ=255)
                                                                     gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGc                                  >  2:295191/1‑101 (MQ=255)
                                                                       gggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCgg                                <  2:3446393/101‑1 (MQ=255)
                                                                        ggCGTGTATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGt                               <  2:3491787/101‑1 (MQ=255)
                                                                        ggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGt                               <  2:3951342/101‑1 (MQ=255)
                                                                        ggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGt                               >  1:3108544/1‑101 (MQ=255)
                                                                        ggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGt                               >  1:1904689/1‑101 (MQ=255)
                                                                        ggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGGG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGt                               >  1:1174167/1‑101 (MQ=255)
                                                                           gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGa                            >  2:2910573/1‑101 (MQ=255)
                                                                            tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGAc                           >  2:4190470/1‑101 (MQ=255)
                                                                             ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGGCa                          >  2:4538520/1‑101 (MQ=255)
                                                                              gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                         >  1:487636/1‑101 (MQ=255)
                                                                              gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                         <  1:4648582/101‑1 (MQ=255)
                                                                               aTCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAgg                        <  1:1705247/101‑1 (MQ=255)
                                                                                 cGAAACTGCCCCCGCGGGCCCTGCCGGTGATCGGTG.ACCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCt                      <  1:3304601/101‑1 (MQ=255)
                                                                                  gAAACTGCCCCCGCTGCCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTg                     <  2:15525/101‑1 (MQ=255)
                                                                                  gAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTg                     >  2:4323186/1‑101 (MQ=255)
                                                                                   aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTgg                    <  2:4250736/101‑1 (MQ=255)
                                                                                   aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTgg                    >  2:1999207/1‑101 (MQ=255)
                                                                                    aaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGt                   >  2:2451969/1‑101 (MQ=255)
                                                                                     aCTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTa                  <  1:2939327/101‑1 (MQ=255)
                                                                                     aCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTa                  >  1:87160/1‑101 (MQ=255)
                                                                                     aCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTa                  <  2:2031517/101‑1 (MQ=255)
                                                                                      ccgCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTaa                 <  2:1174077/99‑1 (MQ=255)
                                                                                      cTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTaa                 <  2:2835698/101‑1 (MQ=255)
                                                                                        gCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACg               >  2:100228/1‑101 (MQ=255)
                                                                                        gCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACg               >  2:472824/1‑101 (MQ=255)
                                                                                         cACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgt              >  1:3903611/1‑101 (MQ=255)
                                                                                          aCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgtg             >  2:745947/1‑101 (MQ=255)
                                                                                           cccGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgtgt            >  1:2529575/1‑101 (MQ=255)
                                                                                             cGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCg          <  2:1128152/101‑1 (MQ=255)
                                                                                               cTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCt        <  2:1873398/101‑1 (MQ=255)
                                                                                                tGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTc       >  1:233603/1‑101 (MQ=255)
                                                                                                 ggCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc      <  1:4307665/101‑1 (MQ=255)
                                                                                                 ggCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc      >  2:3203681/1‑101 (MQ=255)
                                                                                                   cccTGCCGGTGATCGGTG.ATGAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccc    <  1:1543723/101‑1 (MQ=255)
                                                                                                   cccTGCCGGTGATCGGTG.ATCAGCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccc    <  2:1672524/101‑1 (MQ=255)
                                                                                                     |                                                                                                     
CTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCC  >  NC_014034/1395311‑1395511

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG