Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,424 G→T 5.7% intergenic (+236/‑695) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,4240G→T 5.7% 22.2 ‑1.5 ‑0.3195intergenic (+236/‑695)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/7):  ref base (103/80):  total (107/88)
Fisher's exact test strand distribution p-value = 2.25e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.09e-01

Only 100 of 246 total aligned reads displayed.

GAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1395324‑1395523
                                                                                                    |                                                                                                    
   cAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcggtg.a                                                                                                  >  2:1888783/1‑101 (MQ=255)
   cAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcggtg.a                                                                                                  >  2:4648326/1‑101 (MQ=255)
    aaTTTACCCCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCAAAACTGCACCCGCTGGCCCTGCcggtgatcggtg.at                                                                                                 <  1:4025066/101‑1 (MQ=255)
     aTTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCggtgatcggtg.atc                                                                                                <  2:1541292/101‑1 (MQ=255)
     aTTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCggtgatcggtg.atc                                                                                                <  1:2394836/101‑1 (MQ=255)
     aTTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCggggatcggtg.atc                                                                                                >  1:902124/1‑101 (MQ=255)
      tttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCa                                                                                               <  2:4181581/101‑1 (MQ=255)
       ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                                              >  1:3446605/1‑101 (MQ=255)
       ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                                              >  2:4028171/1‑101 (MQ=255)
            ccGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTc                                                                                         >  1:805163/1‑101 (MQ=255)
              gTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGc                                                                                       <  2:901034/101‑1 (MQ=255)
              gTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGc                                                                                       >  1:1128238/1‑101 (MQ=255)
               tCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCt                                                                                      <  1:2950590/101‑1 (MQ=255)
                cTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTc                                                                                     <  2:2509369/101‑1 (MQ=255)
                 tGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTcc                                                                                    >  1:3276057/1‑101 (MQ=255)
                 tGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTcc                                                                                    >  2:3478999/1‑101 (MQ=255)
                   gTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTcccc                                                                                  >  2:859224/1‑101 (MQ=255)
                   gTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTcccc                                                                                  <  2:302489/101‑1 (MQ=255)
                     cAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCGcccccc                                                                                >  1:768114/1‑101 (MQ=255)
                     cAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGCTG.ATCAGCGGTCGCTcccccc                                                                                <  1:2690938/101‑1 (MQ=255)
                      aGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCcg                                                                               >  1:4362631/1‑101 (MQ=255)
                      aGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCcg                                                                               >  1:3334749/1‑101 (MQ=255)
                      aGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCcg                                                                               >  2:3445158/1‑101 (MQ=255)
                        cGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCcgcg                                                                             <  1:1591842/101‑1 (MQ=255)
                           gTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCgggg                                                                          <  2:3851517/101‑1 (MQ=255)
                            ttACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCggggg                                                                         >  2:3899445/1‑101 (MQ=255)
                             tACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGc                                                                        <  1:1773351/101‑1 (MQ=255)
                             tACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGc                                                                        >  2:4307418/1‑101 (MQ=255)
                              aCGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCg                                                                       >  1:2835863/1‑101 (MQ=255)
                                 tcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  >  1:1142741/9‑101 (MQ=2)
                                 tcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  >  2:2662702/9‑101 (MQ=2)
                                 aaaCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTGCCCCCGCGGGGGCGTgg                                                                    >  1:119442/1‑101 (MQ=255)
                                 aaaCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTgg                                                                    >  1:1238378/1‑101 (MQ=255)
                                  aaCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                   <  2:4427497/101‑1 (MQ=255)
                                  aaCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGGGGa                                                                   >  1:1941785/1‑101 (MQ=255)
                                   aCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGGGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGaa                                                                  >  2:839720/1‑100 (MQ=255)
                                    acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                               >  2:2395214/6‑101 (MQ=2)
                                     tcttgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                              <  2:2091458/97‑1 (MQ=2)
                                     cactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                              <  1:550018/97‑1 (MQ=2)
                                        aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGGGTGGAGCGaaa                                                             >  1:2930439/1‑101 (MQ=255)
                                        aGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                             <  1:403347/101‑1 (MQ=255)
                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                            <  2:1576505/101‑1 (MQ=255)
                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                                          <  1:3162291/101‑3 (MQ=2)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                          >  2:2731249/1‑101 (MQ=255)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCCCCCCCCCCGCGGGGGCGTGGATCGAACCAt                                                          >  1:1897572/1‑101 (MQ=255)
                                           cGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                          <  1:3567299/101‑1 (MQ=255)
                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                         >  2:4056848/1‑101 (MQ=255)
                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCGGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                         >  1:4719794/1‑101 (MQ=255)
                                             cTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGGAACATcg                                                        >  1:1743739/1‑101 (MQ=255)
                                              tCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgc                                                       >  2:4343137/1‑101 (MQ=255)
                                               ccccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACATcgcc                                                      >  2:1314802/1‑101 (MQ=255)
                                                cccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGGCGCTCCCCCCCCGGGGGCGAGGAGCGAAACATcgccg                                                     >  2:4262527/1‑101 (MQ=39)
                                                 ccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgccgc                                                    <  1:1042531/101‑1 (MQ=255)
                                                    cgcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCttt                                                 <  2:4602113/101‑1 (MQ=255)
                                                    cgcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCttt                                                 >  2:2443389/1‑101 (MQ=255)
                                                      ggggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTgg                                               <  2:3673472/100‑1 (MQ=255)
                                                      cgGGGGCGTGGATCGAAACTGCGCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTgg                                               <  1:1540902/101‑1 (MQ=255)
                                                       gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGc                                              >  2:295191/1‑101 (MQ=255)
                                                       gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGc                                              >  2:2547667/1‑101 (MQ=255)
                                                       gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGc                                              >  2:2162733/1‑101 (MQ=255)
                                                             gTGGATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGa                                        <  1:1354465/101‑1 (MQ=255)
                                                              tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGAc                                       >  1:3055131/1‑101 (MQ=255)
                                                              tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGAc                                       >  2:4190470/1‑101 (MQ=255)
                                                               ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACa                                      >  1:2032663/1‑101 (MQ=255)
                                                                gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                                     <  1:4648582/101‑1 (MQ=255)
                                                                gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                                     >  1:487636/1‑101 (MQ=255)
                                                                 aTCGAATCTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCTTGGATCGAAACATCGCCGCTTTGGCGGGCGACAgg                                    <  2:2891306/101‑1 (MQ=38)
                                                                 aTCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAgg                                    <  1:1705247/101‑1 (MQ=255)
                                                                  tCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGc                                   <  1:377253/101‑1 (MQ=255)
                                                                   cGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCt                                  <  2:2912912/101‑1 (MQ=255)
                                                                   cGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCt                                  >  1:99373/1‑101 (MQ=255)
                                                                    gAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTg                                 >  1:1802080/1‑101 (MQ=255)
                                                                     aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTgg                                <  2:805090/101‑1 (MQ=255)
                                                                      aaCTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGt                               <  1:2263163/101‑1 (MQ=255)
                                                                      aaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGt                               >  2:2451969/1‑101 (MQ=255)
                                                                        ccgCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTaa                             <  2:1174077/99‑1 (MQ=255)
                                                                        cTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTaa                             <  1:4179039/101‑1 (MQ=255)
                                                                          gCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACg                           >  2:472824/1‑101 (MQ=255)
                                                                           cACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgt                          >  1:3903611/1‑101 (MQ=255)
                                                                           cACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgt                          >  2:2333215/1‑101 (MQ=255)
                                                                           cACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgt                          >  2:3641498/1‑101 (MQ=255)
                                                                             cccGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgtgt                        >  1:2529575/1‑101 (MQ=255)
                                                                              ccGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTc                       <  1:3299465/101‑1 (MQ=255)
                                                                               cGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCg                      <  2:1128152/101‑1 (MQ=255)
                                                                                 cTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGGAACGTGTCGCt                    >  2:4518900/1‑101 (MQ=255)
                                                                                 cTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGAAACGTGTCGCt                    >  1:1570891/1‑101 (MQ=255)
                                                                                  tGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTc                   >  1:233603/1‑101 (MQ=255)
                                                                                  tGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGGAACGTGTCGCTc                   >  2:3247332/1‑101 (MQ=255)
                                                                                   ggCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                  >  2:3203681/1‑101 (MQ=255)
                                                                                    gCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccc                 <  1:1806866/101‑1 (MQ=255)
                                                                                    gCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccc                 <  2:3556248/101‑1 (MQ=255)
                                                                                     cccttagggtgatcggtg.aagGGCGGGCGCCCGCCGCGCGGGGGCGTGGATCGAGACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccc                <  2:1973446/94‑1 (MQ=255)
                                                                                     cccTGCCGGTGATCGGTG.ATGAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccc                <  1:1543723/101‑1 (MQ=255)
                                                                                     cccTGCCGGTGATCGGTG.ATCAGCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccc                <  2:1672524/101‑1 (MQ=255)
                                                                                       cTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc              >  1:141622/1‑101 (MQ=255)
                                                                                            ggtgatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCggg         <  2:2025776/101‑1 (MQ=255)
                                                                                              tgatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCggggg       >  1:3369673/1‑101 (MQ=255)
                                                                                                  cggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTg   <  2:1238288/101‑1 (MQ=255)
                                                                                                   ggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTgg  <  1:3721657/101‑1 (MQ=255)
                                                                                                   ggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTgg  <  2:568243/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
GAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1395324‑1395523

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG