Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,429 C→G 5.9% intergenic (+241/‑690) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,4290C→G 5.9% 20.1 ‑1.9 ‑0.1195intergenic (+241/‑690)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/8):  ref base (103/80):  total (107/88)
Fisher's exact test strand distribution p-value = 1.43e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.49e-01

Only 100 of 241 total aligned reads displayed.

ATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1395329‑1395529
                                                                                                      |                                                                                                    
aTTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCggtgatcggtg.atc                                                                                                      <  2:1541292/101‑1 (MQ=255)
aTTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCggggatcggtg.atc                                                                                                      >  1:902124/1‑101 (MQ=255)
 tttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCa                                                                                                     <  1:3251464/101‑1 (MQ=255)
  tttccccGGCTGGTCCGGGGGTTTCGGAACGGGAGTCGCCCCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCa                                                                                                     <  1:122525/96‑1 (MQ=25)
  ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                                                    >  1:1576605/1‑101 (MQ=255)
    acacCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCg                                                                                                  >  1:2781062/1‑101 (MQ=255)
    acacCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGGG.ATCAGCg                                                                                                  >  2:2692648/1‑101 (MQ=255)
       ccGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTc                                                                                               >  1:805163/1‑101 (MQ=255)
       ccGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGGG.ATCAGCGGTc                                                                                               >  2:2507284/1‑101 (MQ=255)
         gTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGc                                                                                             >  2:4690501/1‑101 (MQ=255)
         gTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGc                                                                                             >  1:1542723/1‑101 (MQ=255)
         gTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGc                                                                                             <  2:901034/101‑1 (MQ=255)
         gTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGGGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGc                                                                                             >  1:1128238/1‑101 (MQ=255)
           cTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTc                                                                                           >  2:2158305/1‑101 (MQ=255)
            tGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTcc                                                                                          >  2:241564/1‑101 (MQ=255)
            tGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTcc                                                                                          >  1:1258899/1‑101 (MQ=255)
            tGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTcc                                                                                          >  2:3478999/1‑101 (MQ=255)
             ggTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTccc                                                                                         <  1:3285908/101‑1 (MQ=255)
              gTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTcccc                                                                                        <  2:302489/101‑1 (MQ=255)
              gTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTcccc                                                                                        >  2:859224/1‑101 (MQ=255)
                cAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCGcccccc                                                                                      >  1:768114/1‑101 (MQ=255)
                      gTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCgggg                                                                                >  2:2603266/1‑101 (MQ=255)
                           gAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTg                                                                           <  1:1745134/101‑1 (MQ=255)
                            tcaacactgTCG.CTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        >  1:1142741/9‑101 (MQ=2)
                            aaaCGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGGGATCGGGG.ATCAGCGGGCGCTCCCCCCGCGGGGGCGTgg                                                                          >  1:1392815/1‑101 (MQ=255)
                              aCGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGGGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGaa                                                                        >  2:839720/1‑100 (MQ=255)
                               cGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       <  2:4131801/101‑1 (MQ=255)
                               acactgTCG.CTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                     >  2:2395214/6‑101 (MQ=2)
                                tcttgTCG.CTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    <  2:2091458/97‑1 (MQ=2)
                                   tgTCG.CTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                                 >  2:3866929/2‑100 (MQ=2)
                                   aGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGGGTGGAGCGaaa                                                                   >  1:2930439/1‑101 (MQ=255)
                                   aGTCG.CCCCCCCCGCGGGGGCGTGGATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   <  1:403347/101‑1 (MQ=255)
                                    gTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                  <  2:1576505/101‑1 (MQ=255)
                                     tCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                                 >  1:1952128/1‑101 (MQ=255)
                                      cG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                >  1:678747/1‑101 (MQ=255)
                                      cG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                >  1:2278449/1‑101 (MQ=255)
                                      cG.CCCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                <  1:3567299/101‑1 (MQ=255)
                                         cTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                              >  1:1567663/1‑101 (MQ=255)
                                         cTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGGGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTcg                                                              >  2:3964064/1‑101 (MQ=255)
                                          tCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgc                                                             >  1:1799584/1‑101 (MQ=255)
                                           ccccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACATcgcc                                                            >  2:1314802/1‑101 (MQ=255)
                                            cccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgccg                                                           <  1:2376186/101‑1 (MQ=255)
                                             ccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgccgc                                                          <  1:1042531/101‑1 (MQ=255)
                                                cgcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCttt                                                       <  1:4162914/101‑1 (MQ=255)
                                                 gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTg                                                      <  1:2125642/101‑1 (MQ=255)
                                                  ggggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTgg                                                     <  2:3673472/100‑1 (MQ=255)
                                                  cgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTgg                                                     <  2:4232139/101‑1 (MQ=255)
                                                   gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGc                                                    >  2:295191/1‑101 (MQ=255)
                                                   gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGc                                                    >  2:2547667/1‑101 (MQ=255)
                                                   gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGGCGCTCCCCCCGCGGGGGCGTCGATCGAAACATCGCCTCCTTggg                                                    >  2:1805213/1‑100 (MQ=38)
                                                    ggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCg                                                   >  2:418600/1‑101 (MQ=255)
                                                     gggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCgg                                                  >  2:4374082/1‑101 (MQ=255)
                                                     gggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCgg                                                  <  2:3446393/101‑1 (MQ=255)
                                                      ggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGt                                                 >  1:1904689/1‑101 (MQ=255)
                                                      ggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGt                                                 >  1:3108544/1‑101 (MQ=255)
                                                       gCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTc                                                >  2:292233/1‑101 (MQ=255)
                                                           ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGGCa                                            >  2:4538520/1‑101 (MQ=255)
                                                            gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                                           >  1:4496414/1‑101 (MQ=255)
                                                            gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                                           >  1:487636/1‑101 (MQ=255)
                                                             aTCGAATCTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCTTGGATCGAAACATCGCCGCTTTGGCGGGCGACAgg                                          <  2:2891306/101‑1 (MQ=38)
                                                              tCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGc                                         <  1:377253/101‑1 (MQ=255)
                                                               cGAAACTGCCCCCGCGGGCCCTGCCGGTGATCGGTG.ACCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCt                                        <  1:3304601/101‑1 (MQ=255)
                                                               cGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCt                                        >  1:99373/1‑101 (MQ=255)
                                                                gAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTg                                       >  1:2466069/1‑101 (MQ=255)
                                                                gAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTg                                       >  2:4323186/1‑101 (MQ=255)
                                                                 aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTgg                                      <  1:261324/101‑1 (MQ=255)
                                                                 aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTgg                                      <  2:805090/101‑1 (MQ=255)
                                                                  aaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGt                                     >  2:2451969/1‑101 (MQ=255)
                                                                    ccgCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTaa                                   <  2:1174077/99‑1 (MQ=255)
                                                                    cTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTaa                                   <  2:2835698/101‑1 (MQ=255)
                                                                     tGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAc                                  <  1:1526329/101‑1 (MQ=255)
                                                                     tGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGGAAc                                  >  1:4724104/1‑101 (MQ=255)
                                                                      gCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACg                                 >  2:472824/1‑101 (MQ=255)
                                                                       cACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgt                                >  2:2333215/1‑101 (MQ=255)
                                                                       cACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgt                                >  2:3641498/1‑101 (MQ=255)
                                                                           cGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCg                            <  2:1128152/101‑1 (MQ=255)
                                                                            gCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGc                           >  1:3366984/1‑101 (MQ=255)
                                                                             cTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGGAACGTGTCGCt                          >  2:4518900/1‑101 (MQ=255)
                                                                              tGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGGAACGTGTCGCTc                         >  2:3247332/1‑101 (MQ=255)
                                                                               ggCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                        >  2:3203681/1‑101 (MQ=255)
                                                                               ggCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                        <  1:4307665/101‑1 (MQ=255)
                                                                                gCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccc                       <  2:3556248/101‑1 (MQ=255)
                                                                                 cccttagggtgatcggtg.aagGGCGGGCGCCCGCCGCGCGGGGGCGTGGATCGAGACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccc                      <  2:1973446/94‑1 (MQ=255)
                                                                                 cccTGCCGGTGATCGGTG.ATCAGCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccc                      <  2:1672524/101‑1 (MQ=255)
                                                                                  ccTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCTAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccccc                     >  1:1693912/1‑101 (MQ=255)
                                                                                    tGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcg                   >  1:371407/1‑101 (MQ=255)
                                                                                    tGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGCAACGTGTCGCTCCCCCcg                   >  1:1706350/1‑101 (MQ=255)
                                                                                        ggtgatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCggg               <  2:2025776/101‑1 (MQ=255)
                                                                                         gtgatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggg              <  1:3381087/101‑1 (MQ=255)
                                                                                         gtgatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggg              >  2:383942/1‑101 (MQ=255)
                                                                                         gtgatcggtg.atcAGCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggg              <  2:4037938/101‑1 (MQ=255)
                                                                                          tgatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCggggg             <  1:295225/101‑1 (MQ=255)
                                                                                          tgatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCggggg             >  2:4296472/1‑101 (MQ=255)
                                                                                           gatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc            >  1:1805842/1‑101 (MQ=255)
                                                                                           gatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc            >  1:1896893/1‑101 (MQ=255)
                                                                                            atcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCg           >  1:389151/1‑101 (MQ=255)
                                                                                              cggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTg         <  2:1238288/101‑1 (MQ=255)
                                                                                               ggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTgg        <  2:568243/101‑1 (MQ=255)
                                                                                                 tg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGAt      >  1:4649752/1‑101 (MQ=255)
                                                                                                     tcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGa   <  1:3479211/101‑1 (MQ=255)
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ATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1395329‑1395529

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG