Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,430 A→C 5.2% intergenic (+242/‑689) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,4300A→C 5.2% 21.0 ‑1.5 ‑0.5212intergenic (+242/‑689)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/7):  ref base (113/88):  total (117/95)
Fisher's exact test strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.35e-01

Only 100 of 234 total aligned reads displayed.

TTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA  >  NC_014034/1395330‑1395530
                                                                                                      |                                                                                                    
tttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCa                                                                                                      <  1:3251464/101‑1 (MQ=255)
tttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCa                                                                                                      <  2:87181/101‑1 (MQ=255)
 tttccccGGCTGGTCCGGGGGTTTCGGAACGGGAGTCGCCCCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCa                                                                                                      <  1:122525/96‑1 (MQ=25)
 ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                                                     >  2:4028171/1‑101 (MQ=255)
 ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                                                     >  1:3446605/1‑101 (MQ=255)
 ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                                                     >  1:1576605/1‑101 (MQ=255)
   acacCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCg                                                                                                   >  1:2781062/1‑101 (MQ=255)
      ccGTCTGGTCAGCGTGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTc                                                                                                <  2:3118557/101‑1 (MQ=255)
      ccGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTc                                                                                                >  1:805163/1‑101 (MQ=255)
      ccGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTc                                                                                                <  1:499883/101‑1 (MQ=255)
       cGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCg                                                                                               >  2:1526228/1‑101 (MQ=255)
        gTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGc                                                                                              >  1:1542723/1‑101 (MQ=255)
        gTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGGGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGc                                                                                              >  1:1128238/1‑101 (MQ=255)
          cTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTc                                                                                            <  2:2509369/101‑1 (MQ=255)
           tGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTcc                                                                                           >  2:3478999/1‑101 (MQ=255)
           tGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTcc                                                                                           >  1:1258899/1‑101 (MQ=255)
            ggTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTccc                                                                                          <  1:3285908/101‑1 (MQ=255)
              tCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTccccc                                                                                        <  1:1565066/101‑1 (MQ=255)
               cAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCGcccccc                                                                                       >  1:768114/1‑101 (MQ=255)
                   gggTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCgg                                                                                   >  1:946309/1‑101 (MQ=255)
                       tACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGc                                                                               >  2:210988/1‑101 (MQ=255)
                        aCGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCg                                                                              <  1:480418/101‑1 (MQ=255)
                        aCGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCg                                                                              >  2:1658087/1‑101 (MQ=255)
                          gAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTg                                                                            <  1:1745134/101‑1 (MQ=255)
                          gAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGGG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTg                                                                            >  1:3764686/1‑101 (MQ=255)
                           tcaacactgTCG.CTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                         >  2:2662702/9‑101 (MQ=2)
                           tcaacactgTCG.CTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                         >  1:1142741/9‑101 (MQ=2)
                            caacactgTCG.CTCCCCCCGCGGGGGCGTGGATTGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                        <  1:4087114/94‑1 (MQ=2)
                              cGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                        <  2:4131801/101‑1 (MQ=255)
                                  tgTCG.CTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                                  >  2:3866929/2‑100 (MQ=2)
                                  aGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGGGGATCGaaa                                                                    >  2:377209/1‑101 (MQ=255)
                                   gTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                   >  2:2404470/1‑101 (MQ=255)
                                    tCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                                  >  1:1952128/1‑101 (MQ=255)
                                     cG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                 >  1:2278449/1‑101 (MQ=255)
                                     cG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                 >  1:678747/1‑101 (MQ=255)
                                     cG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                 >  2:2731249/1‑101 (MQ=255)
                                     cG.CCCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                 <  1:3567299/101‑1 (MQ=255)
                                      g.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCGGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                                >  1:4719794/1‑101 (MQ=255)
                                         tCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgc                                                              >  1:1799584/1‑101 (MQ=255)
                                            ccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgccgc                                                           <  1:1042531/101‑1 (MQ=255)
                                               cgcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCttt                                                        >  2:2443389/1‑101 (MQ=255)
                                                gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTg                                                       <  1:2125642/101‑1 (MQ=255)
                                                 ggggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTgg                                                      <  2:3673472/100‑1 (MQ=255)
                                                 cgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTgg                                                      <  2:4232139/101‑1 (MQ=255)
                                                  gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGc                                                     >  2:295191/1‑101 (MQ=255)
                                                  gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGc                                                     >  2:2547667/1‑101 (MQ=255)
                                                  gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGc                                                     >  2:2162733/1‑101 (MQ=255)
                                                  gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGGCGCTCCCCCCGCGGGGGCGTCGATCGAAACATCGCCTCCTTggg                                                     >  2:1805213/1‑100 (MQ=38)
                                                   ggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCg                                                    >  2:418600/1‑101 (MQ=255)
                                                    gggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCgg                                                   >  2:4374082/1‑101 (MQ=255)
                                                    gggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCgg                                                   <  2:3446393/101‑1 (MQ=255)
                                                     ggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGt                                                  <  2:3951342/101‑1 (MQ=255)
                                                     ggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGt                                                  >  1:1904689/1‑101 (MQ=255)
                                                     ggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGt                                                  >  1:3108544/1‑101 (MQ=255)
                                                      gCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTc                                                 >  2:292233/1‑101 (MQ=255)
                                                        gTGGATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGa                                               <  1:1354465/101‑1 (MQ=255)
                                                        gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGa                                               >  2:2910573/1‑101 (MQ=255)
                                                         tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGAc                                              <  2:1169461/101‑1 (MQ=255)
                                                          ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGGCa                                             >  2:4538520/1‑101 (MQ=255)
                                                           gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                                            <  1:4648582/101‑1 (MQ=255)
                                                            aTCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAgg                                           <  1:1705247/101‑1 (MQ=255)
                                                            aTCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAgg                                           >  1:1601440/1‑101 (MQ=255)
                                                              cGAAACTGCCCCCGCGGGCCCTGCCGGTGATCGGTG.ACCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCt                                         <  1:3304601/101‑1 (MQ=255)
                                                               gAAACTGCCCCCGCTGCCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTg                                        <  2:15525/101‑1 (MQ=255)
                                                               gAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTg                                        >  2:4323186/1‑101 (MQ=255)
                                                                aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTgg                                       <  2:805090/101‑1 (MQ=255)
                                                                aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTgg                                       >  2:1999207/1‑101 (MQ=255)
                                                                aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTgg                                       <  1:261324/101‑1 (MQ=255)
                                                                  aCTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTa                                     <  1:2939327/101‑1 (MQ=255)
                                                                   cTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTaa                                    <  2:2835698/101‑1 (MQ=255)
                                                                    tGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAc                                   <  1:1526329/101‑1 (MQ=255)
                                                                     gCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACg                                  >  2:100228/1‑101 (MQ=255)
                                                                     gCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACg                                  >  2:472824/1‑101 (MQ=255)
                                                                     gCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACg                                  >  1:2495828/1‑101 (MQ=255)
                                                                      cACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgt                                 >  2:3641498/1‑101 (MQ=255)
                                                                           gCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGc                            >  1:3366984/1‑101 (MQ=255)
                                                                           gCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGc                            <  2:1941668/101‑1 (MQ=255)
                                                                            cTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGGAACGTGTCGCt                           >  2:4518900/1‑101 (MQ=255)
                                                                             tGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTc                          >  1:233603/1‑101 (MQ=255)
                                                                             tGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTc                          >  1:3916197/1‑101 (MQ=255)
                                                                             tGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGGAACGTGTCGCTc                          >  2:3247332/1‑101 (MQ=255)
                                                                             tGGCCCTGCCGGTGATCGGTA.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTc                          <  2:119426/101‑1 (MQ=255)
                                                                              ggCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                         <  1:4307665/101‑1 (MQ=255)
                                                                              ggCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                         >  2:3203681/1‑101 (MQ=255)
                                                                               gCCCTGGCGGGGATCGGTG.ATCAGCGGTCCCTCCCCCCGCGGGGCCGCGGCTCGGAACATGGCCGCTTTGGCGGTCGACATCCTGGGGACgtgcgcgtccc                        >  2:236239/1‑93 (MQ=255)
                                                                               gCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccc                        <  2:3556248/101‑1 (MQ=255)
                                                                                  cTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                     <  2:1240379/101‑1 (MQ=255)
                                                                                   tGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccccccc                    >  2:2491608/1‑100 (MQ=255)
                                                                                   tGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcg                    >  1:371407/1‑101 (MQ=255)
                                                                                    gCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcgc                   >  2:846759/1‑101 (MQ=255)
                                                                                     ccGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcgcg                  >  2:617989/1‑101 (MQ=255)
                                                                                        gtgatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggg               <  1:3381087/101‑1 (MQ=255)
                                                                                        gtgatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggg               <  2:4648101/101‑1 (MQ=255)
                                                                                         tgatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCggggg              <  1:295225/101‑1 (MQ=255)
                                                                                         tgatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCggggg              >  2:4296472/1‑101 (MQ=255)
                                                                                          gatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc             >  1:1896893/1‑101 (MQ=255)
                                                                                          gatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc             >  1:1805842/1‑101 (MQ=255)
                                                                                           atcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCg            >  1:389151/1‑101 (MQ=255)
                                                                                              ggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTgg         <  1:436679/101‑1 (MQ=255)
                                                                                                   atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGGTCg     >  2:2088856/1‑101 (MQ=255)
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TTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA  >  NC_014034/1395330‑1395530

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG