Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,499 T→A 19.6% intergenic (+311/‑620) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,4990T→A19.6% 144.5 ‑4.2 ‑1.1246intergenic (+311/‑620)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (13/35):  ref base (93/104):  total (107/139)
Fisher's exact test strand distribution p-value = 1.44e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.30e-02

Only 100 of 275 total aligned reads displayed.

CACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACC  >  NC_014034/1395399‑1395599
                                                                                                    |                                                                                                     
  cccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgtgt                                                                                                     >  1:2529575/1‑101 (MQ=255)
   ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTc                                                                                                    <  1:3299465/101‑1 (MQ=255)
    cGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCg                                                                                                   <  1:1058770/101‑1 (MQ=255)
     gCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGc                                                                                                  <  2:1941668/101‑1 (MQ=255)
      cTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCt                                                                                                 <  2:1873398/101‑1 (MQ=255)
       tGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTc                                                                                                >  1:233603/1‑101 (MQ=255)
        ggCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                                                                                               >  2:3203681/1‑101 (MQ=255)
         gCCCTGGCGGGGATCGGTGATCAGCGGTCCCTCCCCCCGCGGGGCCGCGGCTCGGAACATGGCCGCTTTGGCGGTCGACATCCTGGGGACgtgcgcgtccc                                                                                              >  2:236239/1‑93 (MQ=255)
         gCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccc                                                                                              <  2:3556248/101‑1 (MQ=255)
          cccTGCCGGTGATCGGTGATGAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccc                                                                                             <  1:1543723/101‑1 (MQ=255)
          cccTGCCGGTGATCGGTGATCAGCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccc                                                                                             <  2:1672524/101‑1 (MQ=255)
            cTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                                                                                           <  2:1240379/101‑1 (MQ=255)
              gCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcgc                                                                                         >  2:4520598/1‑101 (MQ=255)
              gCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcgc                                                                                         >  2:846759/1‑101 (MQ=255)
              gCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcgc                                                                                         <  1:1135923/101‑1 (MQ=255)
                cggtgatcggtgatCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgg                                                                                       >  2:2184999/1‑101 (MQ=255)
                   tgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCggggg                                                                                    >  2:4296472/1‑101 (MQ=255)
                   tgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCggggg                                                                                    >  1:3369673/1‑101 (MQ=255)
                    gatcggtgatcAGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc                                                                                   <  2:3131303/101‑1 (MQ=255)
                     atcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCg                                                                                  >  1:389151/1‑101 (MQ=255)
                        ggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTgg                                                                               <  1:436679/101‑1 (MQ=255)
                        ggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTgg                                                                               <  2:568243/101‑1 (MQ=255)
                          ctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                            <  1:4472746/93‑1 (MQ=0)
                           gatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                            <  2:3764451/101‑1 (MQ=255)
                            atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGGTCg                                                                           >  2:2088856/1‑101 (MQ=255)
                            atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                           <  2:1801971/101‑1 (MQ=255)
                             gacgtggTCGCTCCCCCCGAGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                         <  1:2965012/96‑1 (MQ=2)
                              cAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                         >  1:1347619/1‑101 (MQ=255)
                               cgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                       <  1:1768325/98‑1 (MQ=2)
                               aGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                        <  1:506264/101‑1 (MQ=255)
                                gtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                                      >  2:3071933/3‑101 (MQ=2)
                                gCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                       <  2:2780897/101‑1 (MQ=255)
                                gCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                       <  1:3679263/101‑1 (MQ=255)
                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACagc                                                                    >  1:473888/1‑99 (MQ=2)
                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACagc                                                                    >  1:3232382/1‑99 (MQ=2)
                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACagc                                                                    >  1:2236104/1‑99 (MQ=2)
                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                     <  2:2865853/101‑1 (MQ=255)
                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgct                                                                   >  2:4180444/1‑101 (MQ=2)
                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTGTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgct                                                                   <  2:4643080/101‑1 (MQ=2)
                                     cGCTCCCCCCGCGGGGGCGTTGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgcttt                                                                 <  1:4585092/101‑3 (MQ=2)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgcttt                                                                 <  1:3731242/101‑3 (MQ=2)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttc                                                                <  2:2732947/101‑4 (MQ=2)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttc                                                                <  1:1290968/101‑4 (MQ=2)
                                       ccccccccGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttca                                                               <  1:4681955/99‑5 (MQ=0)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACATGGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg.g                                                               >  1:4226000/1‑101 (MQ=255)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg.g                                                               <  1:1249339/101‑1 (MQ=255)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaa                                                              <  1:1704636/101‑6 (MQ=2)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg.gg                                                              >  2:1067898/1‑100 (MQ=255)
                                         ccccccGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaat                                                             <  1:2129148/101‑7 (MQ=0)
                                         ccccccGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaat                                                             <  1:4131840/101‑7 (MQ=0)
                                         ccccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg.ggg                                                             >  2:1197336/1‑99 (MQ=255)
                                          cccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGc                                                            >  2:3505330/1‑101 (MQ=255)
                                           ccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGcc                                                           <  2:946232/101‑1 (MQ=255)
                                             ccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGt                                                         <  1:2491752/101‑1 (MQ=255)
                                                cgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGc                                                      <  1:4323433/101‑1 (MQ=255)
                                                 gggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCt                                                     <  1:418644/101‑1 (MQ=255)
                                                  ggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTc                                                    >  2:4607811/1‑101 (MQ=255)
                                                  ggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTc                                                    <  1:144014/101‑1 (MQ=255)
                                                   gggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCg                                                   >  2:1417046/1‑101 (MQ=255)
                                                        tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAg                                              <  2:4723848/101‑1 (MQ=255)
                                                         ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGTATGCCGTcttcttcttga                                              >  1:585799/1‑91 (MQ=37)
                                                         ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGc                                             >  1:3556051/1‑101 (MQ=255)
                                                          gATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGcc                                            >  1:4532266/1‑101 (MQ=255)
                                                          gATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGcc                                            >  1:392516/1‑101 (MQ=255)
                                                          gATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGcc                                            >  2:2321401/1‑101 (MQ=255)
                                                           aTCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCg                                           >  2:4389503/1‑101 (MQ=255)
                                                            tCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCgg                                          >  2:624837/1‑101 (MQ=255)
                                                               aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCt                                       >  1:2381793/1‑101 (MQ=255)
                                                                   aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCtc                                   >  1:3710328/1‑101 (MQ=255)
                                                                     cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCtcgt                                 <  2:4362384/101‑1 (MQ=255)
                                                                      gccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCtcgtc                                <  2:1143281/101‑1 (MQ=255)
                                                                       ccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCtcgtcg                               <  1:3479559/101‑1 (MQ=255)
                                                                         gcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCt                             <  1:2603418/101‑1 (MQ=255)
                                                                          cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCtcgtcgaac                            <  2:1743634/101‑4 (MQ=255)
                                                                           tttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTcc                           >  1:2914359/1‑101 (MQ=255)
                                                                           tttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTcc                           >  2:3012406/1‑101 (MQ=255)
                                                                              ggCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTccccc                        <  1:1114776/101‑1 (MQ=255)
                                                                               gccgTCGACAGGGGGGTAACGTCTCCATCCACCCGCGGGGGCGTGGATCGAAACTTCTCG.GGGCCGTCGCACGTCCAGCCGGCCTGCTCGTCGCTcccccc                       <  2:1534226/98‑1 (MQ=25)
                                                                                cGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCcg                      >  2:2554847/1‑101 (MQ=255)
                                                                                 ggTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCcgc                     >  1:1826125/1‑101 (MQ=255)
                                                                                 ggTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTAACTCGTCCAGCCGGCCTGGTCGTCGCTCCCCCcgc                     >  2:728669/1‑101 (MQ=255)
                                                                                   tCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCgg                   >  2:2474405/1‑101 (MQ=255)
                                                                                   tCGACAGGCTGGTAACGTGTCACTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCgg                   >  1:2697298/1‑101 (MQ=255)
                                                                                    cGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCggg                  >  2:3903418/1‑101 (MQ=255)
                                                                                     gACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGCCGCGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCgggg                 <  1:4057090/101‑1 (MQ=255)
                                                                                        aGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCg              <  2:183800/101‑1 (MQ=255)
                                                                                            ttggaaCGGGTCGCTCCCCCCGCGGGGGGGGTGAACGAAAAATTTCG.GTGCCGTCGCTTCTCCCCCCGGGCTGGCCCGCGCCCCCCCCCCcggggggcggg          >  2:2005032/5‑95 (MQ=255)
                                                                                            tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGa          <  1:851151/101‑1 (MQ=255)
                                                                                            tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGa          <  2:4496164/101‑1 (MQ=255)
                                                                                            tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGa          >  1:1906517/1‑101 (MQ=255)
                                                                                            tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGa          <  1:3417473/101‑1 (MQ=255)
                                                                                            tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGa          >  2:3412705/1‑101 (MQ=255)
                                                                                            tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGa          >  1:2848444/1‑101 (MQ=255)
                                                                                            tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGa          <  1:3077695/101‑1 (MQ=255)
                                                                                             ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGAt         <  1:1417140/101‑1 (MQ=255)
                                                                                              gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATc        <  1:1658187/101‑1 (MQ=255)
                                                                                              gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATc        >  2:3344045/1‑101 (MQ=255)
                                                                                                 aCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa     <  2:4036573/101‑1 (MQ=255)
                                                                                                   gtgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac   <  2:3862122/101‑1 (MQ=255)
                                                                                                    tgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc  <  2:2034110/101‑1 (MQ=255)
                                                                                                    |                                                                                                     
CACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACC  >  NC_014034/1395399‑1395599

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG