Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,537 G→T 2.4% intergenic (+349/‑582) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5370G→T 2.4% 11.6 ‑1.2 ‑0.8339intergenic (+349/‑582)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/6):  ref base (160/171):  total (162/177)
Fisher's exact test strand distribution p-value = 2.87e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.54e-01

Only 100 of 366 total aligned reads displayed.

GCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGC  >  NC_014034/1395437‑1395637
                                                                                                    |                                                                                                       
gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg                                                                                                         <  1:1280079/101‑1 (MQ=255)
      cccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GGGCCg                                                                                                  >  2:1881090/1‑101 (MQ=255)
           gggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCt                                                                                             >  1:4440386/1‑101 (MQ=255)
            ggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTc                                                                                            <  2:3054943/101‑1 (MQ=255)
               gCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.Tc                                                                                        >  1:1682559/1‑101 (MQ=255)
                cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.Tcc                                                                                       <  1:141908/101‑1 (MQ=255)
                cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.Tcc                                                                                       >  1:1484511/1‑101 (MQ=255)
                 gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCa                                                                                      <  2:4350687/101‑1 (MQ=255)
                 gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCa                                                                                      >  1:4107/1‑101 (MQ=255)
                  tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACTTGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACATCTCG.GGGCCGTCCCTCG.Tccgc                                                                                     >  1:3834441/1‑99 (MQ=38)
                  tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATTTCG.GAGCGGTCGCTCG.TCCAg                                                                                     >  1:3183057/1‑101 (MQ=255)
                  tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAg                                                                                     <  2:4723848/101‑1 (MQ=255)
                   ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGTATGCCGTcttcttcttga                                                                                      >  1:585799/1‑91 (MQ=37)
                   ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGc                                                                                    >  1:3556051/1‑101 (MQ=255)
                    gATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGcc                                                                                   >  1:392516/1‑101 (MQ=255)
                    gATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGcc                                                                                   >  2:2321401/1‑101 (MQ=255)
                    gATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGcc                                                                                   >  1:4532266/1‑101 (MQ=255)
                       cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTACCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCAGG.c                                                                               >  2:3911077/1‑101 (MQ=255)
                          aaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTg                                                                            >  2:2722552/1‑101 (MQ=255)
                             aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCtc                                                                         <  2:3683929/101‑1 (MQ=255)
                              tcgcgtcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCtcg                                                                        <  1:4171373/95‑1 (MQ=255)
                              tCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCtcg                                                                        <  1:2158429/101‑1 (MQ=255)
                              tCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCtcg                                                                        >  1:2496190/1‑101 (MQ=255)
                               cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCtcgt                                                                       >  2:45632/1‑101 (MQ=255)
                                gccgcTTTGGCGGTGGACAGGCTGGTATCGTGCCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCtcgtc                                                                      <  2:4484770/101‑1 (MQ=38)
                                  cgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGc                                                                    >  2:1463637/1‑101 (MQ=255)
                                    cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTc                                                                  <  1:2547819/101‑1 (MQ=255)
                                      ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTccc                                                                <  1:2404605/101‑1 (MQ=255)
                                       tGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTcccc                                                               >  1:335522/1‑101 (MQ=255)
                                         gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTcccccc                                                             <  1:2333348/101‑1 (MQ=255)
                                            gTCGACAGGCTGTTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAATCATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCcgcg                                                          <  1:1118426/101‑1 (MQ=255)
                                               gACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGCCGCGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCgggg                                                       <  1:4057090/101‑1 (MQ=255)
                                                     cTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTgg                                                 <  2:2028911/101‑1 (MQ=255)
                                                     agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAG.CCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                 <  1:2630725/91‑1 (MQ=0)
                                                      tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGa                                                >  1:2848444/1‑101 (MQ=255)
                                                      tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGa                                                <  1:2088977/101‑1 (MQ=255)
                                                      tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGa                                                <  1:3077695/101‑1 (MQ=255)
                                                      tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGa                                                <  1:851151/101‑1 (MQ=255)
                                                      gcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAG.CCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGa                                                >  2:409185/10‑101 (MQ=0)
                                                      gcggccccggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAG.CCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGa                                                <  2:1918271/92‑1 (MQ=2)
                                                      aggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGa                                                <  2:3843602/92‑1 (MQ=0)
                                                      aggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGa                                                >  1:4397640/10‑101 (MQ=0)
                                                       cggcggcgggcGCCCCCCCCGCGGGGGCGTGGATCGAAACAAAAAG.CCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGAt                                               <  1:2120220/91‑1 (MQ=2)
                                                        ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCG.GCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCCCGGGGGCGTGGGTc                                              >  1:4022789/8‑101 (MQ=2)
                                                         tcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCG.GCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                             <  1:1877516/95‑1 (MQ=2)
                                                         ccgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCG.GCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                             <  2:820492/95‑1 (MQ=2)
                                                          cggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                            <  1:3770669/96‑1 (MQ=2)
                                                          cggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCCGGGCCGTGGATCGa                                            >  2:1383468/6‑101 (MQ=2)
                                                          cgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCG.GCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGGGTGGATCGa                                            >  2:1401152/6‑101 (MQ=2)
                                                          aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                            <  1:2722706/101‑1 (MQ=255)
                                                           ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                           <  2:2992313/97‑1 (MQ=2)
                                                           gcaagtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCG.GCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                           <  1:625392/97‑1 (MQ=2)
                                                           aCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                           <  2:4036573/101‑1 (MQ=255)
                                                           aCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                           <  2:1836224/101‑1 (MQ=255)
                                                            gtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                          <  2:661866/98‑1 (MQ=2)
                                                            cacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCG.GCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                          <  2:2223266/98‑1 (MQ=2)
                                                            cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                          >  1:2920989/1‑101 (MQ=255)
                                                             tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                         >  1:3260484/3‑101 (MQ=2)
                                                             gtggCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                         <  1:3641727/101‑1 (MQ=255)
                                                              tgtCGCTCCCGCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCACG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                        <  2:1258809/101‑1 (MQ=255)
                                                              tgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                        >  2:2840177/1‑101 (MQ=255)
                                                              ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAG.CCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                        <  1:4275305/100‑1 (MQ=2)
                                                              ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                        <  2:4662310/100‑2 (MQ=2)
                                                              ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                        <  1:717412/100‑2 (MQ=2)
                                                              ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                        <  2:1065177/100‑2 (MQ=2)
                                                              ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                        <  1:968833/100‑2 (MQ=2)
                                                              ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTACCCCCGCGGGGGCGTGGATCGAAact                                        >  2:632360/2‑100 (MQ=2)
                                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCG.GCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgc                                      <  2:3063711/101‑4 (MQ=2)
                                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactat                                      >  2:706388/1‑98 (MQ=2)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                     <  1:4343384/101‑1 (MQ=255)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAG.CCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccg                                     <  2:2415829/101‑4 (MQ=2)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                     >  1:3459559/1‑97 (MQ=2)
                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                   <  2:2465926/101‑1 (MQ=255)
                                                                    cccccccGCGGGGGCGTGGATCGAAACAAAAAG.CCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcgg                                  <  2:749696/100‑7 (MQ=2)
                                                                     ccccccGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGTTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGc                                 >  2:4610531/1‑101 (MQ=255)
                                                                     ccccccGCGGGGGCGTGGATCGAAACAAAAAG.CCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                 >  1:748816/1‑94 (MQ=2)
                                                                     ccccccGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                 <  1:195284/101‑9 (MQ=2)
                                                                     ccccccGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                 <  1:1383562/101‑9 (MQ=2)
                                                                      cccccGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggc                                <  2:1724250/101‑10 (MQ=2)
                                                                       ccccGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggct                               <  2:516424/101‑11 (MQ=2)
                                                                           gcgGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTg                           >  1:1020859/1‑101 (MQ=255)
                                                                            cgGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                          >  2:926402/1‑101 (MQ=255)
                                                                            cgGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                          >  1:490933/1‑101 (MQ=255)
                                                                             gggggCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                         >  2:3901245/1‑101 (MQ=255)
                                                                             gggggCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                         <  2:437634/101‑1 (MQ=255)
                                                                                 gCGTGGAGCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACtt                     <  2:3360026/101‑1 (MQ=255)
                                                                                  cGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                    <  2:3334547/101‑1 (MQ=255)
                                                                                  cGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                    <  1:3505542/101‑1 (MQ=255)
                                                                                    tGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                  >  2:2536137/1‑101 (MQ=255)
                                                                                    tGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                  >  1:4210841/1‑101 (MQ=255)
                                                                                     ggATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgc                 >  1:1247793/1‑101 (MQ=255)
                                                                                      gATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGGTCCTTTcgcg                >  2:163983/1‑101 (MQ=255)
                                                                                           aaaCATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCg           >  1:2309426/1‑101 (MQ=255)
                                                                                            aaCATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGa          <  2:3469272/101‑1 (MQ=255)
                                                                                            aaCATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGa          >  2:2168050/1‑101 (MQ=255)
                                                                                             aCATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGaa         <  2:3154186/101‑1 (MQ=255)
                                                                                                   cG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTcc   >  2:3724563/1‑100 (MQ=255)
                                                                                                   cG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCg   >  1:1908169/1‑101 (MQ=255)
                                                                                                   cG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCg   <  1:3412911/101‑1 (MQ=255)
                                                                                                   cG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCg   <  2:112374/101‑1 (MQ=255)
                                                                                                    |                                                                                                       
GCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGC  >  NC_014034/1395437‑1395637

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG