Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,538 G→C 8.6% intergenic (+350/‑581) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5380G→C 8.6% 58.2 ‑1.1 ‑0.0338intergenic (+350/‑581)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (11/18):  ref base (150/159):  total (161/177)
Fisher's exact test strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.42e-01

Only 100 of 366 total aligned reads displayed.

CTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCT  >  NC_014034/1395438‑1395638
                                                                                                    |                                                                                                       
  ccccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCgggg                                                                                                       >  2:1197336/1‑99 (MQ=255)
   cccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGc                                                                                                      >  2:3505330/1‑101 (MQ=255)
         cgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGc                                                                                                >  1:3862364/1‑101 (MQ=255)
                gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                        <  2:4350687/101‑1 (MQ=255)
                gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGGGCCGTCGCTCG.CCCa                                                                                        >  1:486666/1‑101 (MQ=255)
                 tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAg                                                                                       <  2:4723848/101‑1 (MQ=255)
                 tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAg                                                                                       >  2:1114690/1‑101 (MQ=255)
                 tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAg                                                                                       <  1:1314894/101‑1 (MQ=255)
                  ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGc                                                                                      >  1:1440097/1‑101 (MQ=255)
                      cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.c                                                                                 >  1:138751/1‑101 (MQ=255)
                      cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCCCTCG.TCCAGCCCG.c                                                                                 >  1:4485020/1‑101 (MQ=255)
                      cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTACCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCAGG.c                                                                                 >  2:3911077/1‑101 (MQ=255)
                            aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCtc                                                                          <  1:211013/101‑1 (MQ=255)
                            aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCtc                                                                          >  1:3710328/1‑101 (MQ=255)
                              cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCtcgt                                                                        >  2:45632/1‑101 (MQ=255)
                              cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCACCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCtcgt                                                                        <  1:3195144/101‑1 (MQ=255)
                               gccgcTTTGGCGGTGGACAGGCTGGTATCGTGCCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCtcgtc                                                                       <  2:4484770/101‑1 (MQ=38)
                                ccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCtcgtcg                                                                      <  2:3366782/101‑1 (MQ=255)
                                   cTTTGGCGGTGGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTc                                                                   <  2:1430966/101‑1 (MQ=255)
                                    tttGGCGGTCGACAGGCTGGTAGCGTGTCGCTCCCCCCGCGGGGGCGTGAATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTcc                                                                  <  2:1904577/101‑1 (MQ=255)
                                     ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTccc                                                                 <  2:1952011/101‑1 (MQ=255)
                                     ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTccc                                                                 >  2:3852775/1‑101 (MQ=255)
                                      tGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTcccc                                                                >  2:2190431/1‑101 (MQ=255)
                                          ggTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCcgc                                                            >  2:2775359/1‑101 (MQ=255)
                                           gTCGACAGGCTGTTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAATCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCcgcg                                                           <  1:1118426/101‑1 (MQ=255)
                                               aCAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCTCggggg                                                       >  1:2371916/1‑101 (MQ=255)
                                                cAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGc                                                      <  1:4190716/101‑1 (MQ=255)
                                                  ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCgg                                                    >  1:437678/1‑100 (MQ=255)
                                                  ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGt                                                    <  1:2910749/101‑1 (MQ=255)
                                                    agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                  <  1:2688841/91‑1 (MQ=0)
                                                     gcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGa                                                 >  2:409185/10‑101 (MQ=0)
                                                      ggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGAt                                                <  1:3627484/93‑1 (MQ=2)
                                                       gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCATCTCGGTGCCGTCGCTCT.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                               <  1:4063580/101‑1 (MQ=255)
                                                       gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                               <  2:1805733/101‑1 (MQ=255)
                                                       gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                               >  2:3344045/1‑101 (MQ=255)
                                                       ataccgcgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.ATCGGCCGG.GCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                <  1:808751/94‑1 (MQ=0)
                                                        tcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                              <  1:4293659/95‑1 (MQ=2)
                                                        tcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                              <  1:3367852/95‑1 (MQ=2)
                                                        taccgcgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.ATCGGCCGG.GCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATc                                               <  1:347539/95‑1 (MQ=2)
                                                         accgcgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.ATCGGCCGG.GCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                              >  1:4254342/6‑101 (MQ=2)
                                                          ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGGGGATCGaa                                            >  2:3022902/5‑101 (MQ=2)
                                                          gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                            <  2:3131808/97‑1 (MQ=2)
                                                          aCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                            <  2:1693807/101‑1 (MQ=255)
                                                          aCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                            <  2:4036573/101‑1 (MQ=255)
                                                           gtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                           <  2:661866/98‑1 (MQ=2)
                                                           gtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                           <  2:1559291/98‑1 (MQ=2)
                                                            tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                          >  1:3543498/3‑101 (MQ=2)
                                                            tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                          >  1:540009/3‑101 (MQ=2)
                                                            gtgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGGGTGGATCGAAac                                          >  2:1008457/1‑101 (MQ=255)
                                                             tgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                         <  1:4142770/101‑1 (MQ=255)
                                                             ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                         <  2:4662310/100‑2 (MQ=2)
                                                             ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTACCCCCGCGGGGGCGTGGATCGAAact                                         >  2:632360/2‑100 (MQ=2)
                                                              gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTAGCTCCCCCCGCGGGGGCGTGGATCGAAactg                                        >  2:2257579/1‑99 (MQ=2)
                                                               tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccac                                       >  1:259179/1‑101 (MQ=255)
                                                               tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactat                                       >  2:706388/1‑98 (MQ=2)
                                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                      <  1:2507428/101‑1 (MQ=255)
                                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccg                                      >  1:1905821/1‑98 (MQ=2)
                                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccg                                      <  1:1972686/101‑4 (MQ=2)
                                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccg                                      >  1:2152533/1‑98 (MQ=2)
                                                                   tCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                   >  2:849138/1‑101 (MQ=255)
                                                                   tCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTACCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                   >  2:3749675/1‑101 (MQ=255)
                                                                   tCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                                   >  2:1389211/1‑94 (MQ=2)
                                                                   tCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                                   >  2:2490442/1‑94 (MQ=2)
                                                                   cccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcgg                                   <  2:749696/100‑7 (MQ=2)
                                                                    ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGTTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGc                                  >  2:4610531/1‑101 (MQ=255)
                                                                     cccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggc                                 >  1:1926160/1‑92 (MQ=2)
                                                                     cccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggc                                 <  2:2455227/101‑10 (MQ=2)
                                                                      ccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggcgt                                <  1:932470/101‑10 (MQ=2)
                                                                      ccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggct                                <  2:516424/101‑11 (MQ=2)
                                                                       cccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGa                               <  1:4432207/101‑1 (MQ=255)
                                                                       cccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggcgtc                               <  1:3424040/101‑11 (MQ=2)
                                                                        ccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAt                              <  1:1805322/101‑1 (MQ=255)
                                                                        ccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAt                              >  2:3237893/1‑101 (MQ=255)
                                                                          gcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTg                            >  2:306249/1‑101 (MQ=255)
                                                                          gcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTg                            >  1:1020859/1‑101 (MQ=255)
                                                                          gcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTg                            >  1:2424714/1‑101 (MQ=255)
                                                                           cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                           >  2:926402/1‑101 (MQ=255)
                                                                           cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                           <  1:619124/101‑1 (MQ=255)
                                                                           cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                           >  2:2939002/1‑101 (MQ=255)
                                                                            gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                          >  2:3901245/1‑101 (MQ=255)
                                                                            gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                          <  2:437634/101‑1 (MQ=255)
                                                                            gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCACGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                          >  1:4052394/1‑101 (MQ=255)
                                                                             ggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCa                         <  1:2321534/101‑1 (MQ=255)
                                                                              gggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCAc                        >  1:3052483/1‑101 (MQ=255)
                                                                              gggCGTGGATCGAAACATCTCGGTGCCGGCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCAc                        <  1:3811910/101‑1 (MQ=255)
                                                                               ggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACt                       <  2:3710098/101‑1 (MQ=255)
                                                                               ggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACt                       >  1:3154386/1‑101 (MQ=255)
                                                                                gCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GTTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACtt                      <  2:2618830/101‑1 (MQ=255)
                                                                                gCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACtt                      <  1:746021/101‑1 (MQ=255)
                                                                                gCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACtt                      <  1:3964306/101‑1 (MQ=255)
                                                                                    ggATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgc                  >  1:1247793/1‑101 (MQ=255)
                                                                                    ggATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgc                  >  1:1527343/1‑101 (MQ=255)
                                                                                        cGAAACATCTCGGGGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTTTCACTTTCGCgggg              >  1:713683/1‑101 (MQ=255)
                                                                                          aaaCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCg            >  1:3322796/1‑101 (MQ=255)
                                                                                          aaaCAGCTCGGTGCCGTCGCTCG.TCCAGCCGG.GCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCg            <  2:2111782/101‑1 (MQ=255)
                                                                                            aCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGaa          <  2:2381659/101‑1 (MQ=255)
                                                                                              aTCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGCTTGTCACTTTCGCGGGGGGAAGc        >  2:1867702/1‑101 (MQ=255)
                                                                                               tctcGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCg       <  1:2840342/101‑1 (MQ=255)
                                                                                                   ggTGCTGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTAGc   >  2:1280834/1‑101 (MQ=255)
                                                                                                   ggTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGc   <  1:2167422/101‑1 (MQ=255)
                                                                                                    |                                                                                                       
CTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCT  >  NC_014034/1395438‑1395638

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG