Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,544 T→C 21.9% intergenic (+356/‑575) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5440T→C21.9% 126.0 ‑0.3 ‑1.1342intergenic (+356/‑575)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (23/27):  ref base (109/112):  total (161/181)
Fisher's exact test strand distribution p-value = 7.55e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.10e-02

Only 100 of 369 total aligned reads displayed.

CCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCC  >  NC_014034/1395444‑1395644
                                                                                                     |                                                                                                      
ccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGt                                                                                                        <  1:2491752/101‑1 (MQ=255)
     ggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTc                                                                                                   <  1:144014/101‑1 (MQ=255)
          gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCa                                                                                             <  2:4350687/101‑1 (MQ=255)
          gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCa                                                                                             >  1:4107/1‑101 (MQ=255)
           tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACTTGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACATCTCG.GGGCCGTCCCTCG.Tccgc                                                                                            >  1:3834441/1‑99 (MQ=38)
           tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATTTCG.GAGCGGTCGCTCG.TCCAg                                                                                            >  1:3183057/1‑101 (MQ=255)
           tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAg                                                                                            <  2:4723848/101‑1 (MQ=255)
            ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGTATGCCGTcttcttcttga                                                                                             >  1:585799/1‑91 (MQ=37)
            ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGc                                                                                           >  1:3556051/1‑101 (MQ=255)
             gATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGcc                                                                                          >  1:392516/1‑101 (MQ=255)
             gATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGcc                                                                                          >  1:4532266/1‑101 (MQ=255)
               tCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCgg                                                                                        >  2:624837/1‑101 (MQ=255)
                cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTACCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCAGG.c                                                                                      >  2:3911077/1‑101 (MQ=255)
                  aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCt                                                                                    >  1:2381793/1‑101 (MQ=255)
                     cATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCt                                                                                 >  1:501187/1‑101 (MQ=255)
                      aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCtc                                                                                <  1:211013/101‑1 (MQ=255)
                       tcgcgtcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCtcg                                                                               <  1:4171373/95‑1 (MQ=255)
                        cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCtcgt                                                                              >  2:45632/1‑101 (MQ=255)
                        cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCtcgt                                                                              <  2:3369467/101‑1 (MQ=255)
                             cTTTGGCGGTGGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTc                                                                         <  2:1430966/101‑1 (MQ=255)
                             cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTc                                                                         <  1:2547819/101‑1 (MQ=255)
                             cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTc                                                                         <  2:4649496/101‑1 (MQ=255)
                                tGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTcccc                                                                      >  2:2190431/1‑101 (MQ=255)
                                    ggTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCcgc                                                                  >  2:2775359/1‑101 (MQ=255)
                                    ggTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCcgc                                                                  >  1:1826125/1‑101 (MQ=255)
                                     gTCGACAGGCTGTTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAATCATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCcgcg                                                                 <  1:1118426/101‑1 (MQ=255)
                                        gACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGCCGCGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCgggg                                                              <  1:4057090/101‑1 (MQ=255)
                                              gccctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCTAAACAATGCG.GCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTgg                                                        <  1:1389997/91‑1 (MQ=0)
                                               ttggaaCGGGTCGCTCCCCCCGCGGGGGGGGTGAACGAAAAATTTCG.GTGCCGTCGCTTC.TCCCCCCGG.GCTGGCCCGCGCCCCCCCCCCcggggggcggg                                                       >  2:2005032/5‑95 (MQ=255)
                                               tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGa                                                       >  1:1906517/1‑101 (MQ=255)
                                               tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGa                                                       <  1:851151/101‑1 (MQ=255)
                                               tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGa                                                       >  1:2848444/1‑101 (MQ=255)
                                               gcggccccggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAG.CCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGa                                                       <  2:1918271/92‑1 (MQ=2)
                                               aggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGa                                                       >  2:650988/10‑101 (MQ=0)
                                               aggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGa                                                       <  2:3843602/92‑1 (MQ=0)
                                                cctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCG.GCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                      <  1:1379118/93‑1 (MQ=2)
                                                 gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                     >  2:3344045/1‑101 (MQ=255)
                                                 gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                     <  1:1658187/101‑1 (MQ=255)
                                                 gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                     <  2:1805733/101‑1 (MQ=255)
                                                 ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCG.GCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCCCGGGGGCGTGGGTc                                                     >  1:4022789/8‑101 (MQ=2)
                                                  ccgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCG.GCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    <  2:820492/95‑1 (MQ=2)
                                                   cggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                   <  1:3770669/96‑1 (MQ=2)
                                                   cggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGGGGATCGa                                                   >  2:4411374/6‑101 (MQ=2)
                                                   aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                   <  1:2722706/101‑1 (MQ=255)
                                                    ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                  <  2:1255194/97‑1 (MQ=2)
                                                    ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGGGGATCGaa                                                  >  2:3022902/5‑101 (MQ=2)
                                                    gcaagtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCG.GCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                  <  1:625392/97‑1 (MQ=2)
                                                    aCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                  <  2:1693807/101‑1 (MQ=255)
                                                     gtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 <  2:1559291/98‑1 (MQ=2)
                                                     cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 >  1:2920989/1‑101 (MQ=255)
                                                      tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                >  1:3260484/3‑101 (MQ=2)
                                                      gtggCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                <  1:3641727/101‑1 (MQ=255)
                                                       ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                               <  2:1065177/100‑2 (MQ=2)
                                                        gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCG.GCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTAGCTCCCCCCGCGGGGGCGTGGATCGAAactg                                              >  2:2257579/1‑99 (MQ=2)
                                                        gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacta                                              <  1:4370427/101‑3 (MQ=2)
                                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccac                                             >  2:4085738/1‑101 (MQ=255)
                                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAG.CCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccc                                             <  1:1944642/101‑3 (MQ=2)
                                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                            >  1:3459559/1‑97 (MQ=2)
                                                            cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                          <  1:728742/101‑1 (MQ=255)
                                                            cTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAG.CCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcg                                          >  1:2674708/1‑96 (MQ=2)
                                                            cTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgct                                          <  1:4457710/101‑7 (MQ=2)
                                                             tCCCCCCGCGGGGGCGTGGATCGAAACAATGCG.GCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                                         >  2:2490442/1‑94 (MQ=2)
                                                             tCCCCCCGCGGGGGCGTGGATCGAAACAAAAAG.CCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcgg                                         >  1:4426174/1‑95 (MQ=2)
                                                              ccccccGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGTTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGc                                        >  2:4610531/1‑101 (MQ=255)
                                                              ccccccGCGGGGGCGTGGATCGAAACAATGCG.GCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcg                                        <  1:2019122/101‑9 (MQ=2)
                                                              ccccccGCGGGGGCGTGGATCGAAACAAAAAG.CCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                        >  1:748816/1‑94 (MQ=2)
                                                              ccccccGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                        <  2:314758/101‑9 (MQ=2)
                                                               cccccGCGGGGGCGTGGATCGAAACAATGCG.GCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcgc                                       <  1:4289501/101‑10 (MQ=2)
                                                               cccccGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggc                                       <  2:2455227/101‑10 (MQ=2)
                                                                ccccGCGGGGGCGTGGATCGAAACAATGCG.GCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcgcg                                      >  2:684512/1‑91 (MQ=2)
                                                                ccccGCGGGGGCGTGGATCGAAACA.CCCG.GCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggct                                      <  2:516424/101‑11 (MQ=2)
                                                                  ccGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAt                                    >  2:3237893/1‑101 (MQ=255)
                                                                    gcgGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTg                                  >  2:306249/1‑101 (MQ=255)
                                                                    gcgGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTg                                  >  1:1020859/1‑101 (MQ=255)
                                                                     cgGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                                 >  2:2939002/1‑101 (MQ=255)
                                                                      gggggCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                                <  2:437634/101‑1 (MQ=255)
                                                                       ggggCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCa                               <  1:2321534/101‑1 (MQ=255)
                                                                       ggggCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCa                               <  1:994310/101‑1 (MQ=255)
                                                                        gggCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCAc                              >  1:3052483/1‑101 (MQ=255)
                                                                         ggCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACt                             <  2:3710098/101‑1 (MQ=255)
                                                                          gCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGTTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACtt                            <  2:2618830/101‑1 (MQ=255)
                                                                           cGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                           <  1:3505542/101‑1 (MQ=255)
                                                                            gTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGAGCGAAACCCCCTTGCGCATTGTCTCTTTc                          >  1:2152672/1‑101 (MQ=38)
                                                                             tGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                         >  1:4210841/1‑101 (MQ=255)
                                                                              ggATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgc                        >  1:1247793/1‑101 (MQ=255)
                                                                                    aaaCATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCg                  >  1:333957/1‑101 (MQ=255)
                                                                                    aaaCAGCTCG.GTGCCGTCGCTCG.TCCAGCCGG.GCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCg                  <  2:2111782/101‑1 (MQ=255)
                                                                                      aCATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGaa                <  2:392472/101‑1 (MQ=255)
                                                                                      aCATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGaa                <  2:2381659/101‑1 (MQ=255)
                                                                                        aTCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGCTTGTCACTTTCGCGGGGGGAAGc              >  2:1867702/1‑101 (MQ=255)
                                                                                        aTCTCG.GGGCCGTCGCTCG.TCCGGCCGG.CCTGCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTGACTTTCGCggggtgtttc              >  2:720529/1‑95 (MQ=37)
                                                                                            cG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCg          <  2:138735/101‑1 (MQ=255)
                                                                                            cG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCg          <  1:3412911/101‑1 (MQ=255)
                                                                                             g.gTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGc         <  1:2167422/101‑1 (MQ=255)
                                                                                               gTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCt        >  2:388760/1‑101 (MQ=255)
                                                                                               gTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCt        <  2:902050/101‑1 (MQ=255)
                                                                                                tGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTc       >  1:2423082/1‑101 (MQ=255)
                                                                                                   cGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccc    >  2:3758313/1‑101 (MQ=255)
                                                                                                    gTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTccccc   >  2:1322081/1‑101 (MQ=255)
                                                                                                     tCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccccc  >  2:349316/1‑101 (MQ=255)
                                                                                                     |                                                                                                      
CCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCC  >  NC_014034/1395444‑1395644

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG