BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,668	T→C	6.3%	intergenic (+480/‑451)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,668	0	T→C	6.3%	26.3 ‑0.8 ‑0.2	239	intergenic (+480/‑451)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (6/9): ref base (116/108): total (122/117)
Fisher's exact test strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.78e-01

Only 100 of 270 total aligned reads displayed.


TCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGC  >  NC_014034/1395568‑1395768


 cgCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTg                                                                                                       <  1:2005149/101‑1 (MQ=255)


     ccccccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATg                                                                                                   <  1:100244/101‑1 (MQ=255)


      cccccGCGGGGGCGTGGATCGAAGCCACCTTGTGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGt                                                                                                  <  2:4532013/101‑1 (MQ=255)


      cccccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGt                                                                                                  <  1:4374329/101‑1 (MQ=255)


       ccccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTa                                                                                                 <  1:4389750/101‑1 (MQ=255)


           gcgGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcg                                                                                             <  2:821703/101‑1 (MQ=255)


           gcgGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcg                                                                                             >  2:3181578/1‑101 (MQ=255)


               gggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcggcgg                                                                                         <  1:3344247/101‑1 (MQ=255)


                ggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCgg.g                                                                                       <  1:1457910/101‑1 (MQ=255)


                  cGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCa                                                                                     <  2:389107/101‑1 (MQ=255)


                   gTGGATCGAAACCACCTTGCGGATTTTCCCTTTTGCGGGGCGAAGCTTCGCTTCCCCCGCGGGGGCGAGGCTCGAAACATCTGAATGCATTCGGCAG.GCAg                                                                                    >  2:3905047/1‑101 (MQ=255)


                    tGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGt                                                                                   <  1:1674427/101‑1 (MQ=255)


                     ggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGtt                                                                                  >  1:175483/1‑101 (MQ=255)


                      gATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTc                                                                                 <  2:2920813/101‑1 (MQ=255)


                      gATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGCGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTc                                                                                 >  2:4722053/1‑101 (MQ=255)


                       aTCGAAACCGCCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCg                                                                                <  1:2536282/101‑1 (MQ=255)


                       aTCGAAACCCCCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCg                                                                                <  1:849212/101‑1 (MQ=255)


                       aTCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCg                                                                                <  1:4666545/101‑1 (MQ=255)


                       aTCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCg                                                                                <  2:1799475/101‑1 (MQ=255)


                        tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGa                                                                               >  1:6287/1‑101 (MQ=255)


                        tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGa                                                                               >  2:1739953/1‑101 (MQ=255)


                        tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGa                                                                               >  1:1291487/1‑101 (MQ=255)


                        tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGa                                                                               >  2:3511641/1‑101 (MQ=255)


                         cGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.g                                                                             >  2:1268809/1‑101 (MQ=255)


                          gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.Gc                                                                            >  2:1578434/1‑101 (MQ=255)


                           aaaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.Gcc                                                                           >  1:4578792/1‑101 (MQ=255)


                             accaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGc                                                                         >  1:1049710/1‑101 (MQ=255)


                             accaccTTGCGGATTGTCACTTTCGCGGGGCGAACCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGc                                                                         <  2:2371783/101‑1 (MQ=255)


                              cccccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCa                                                                        <  1:3054796/98‑1 (MQ=255)


                              ccaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCa                                                                        <  1:3181778/101‑1 (MQ=255)


                              ccaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCa                                                                        >  2:3385800/1‑101 (MQ=255)


                                  cTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTcc                                                                    >  2:4468960/1‑100 (MQ=255)


                                  cTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCg                                                                    >  1:2898617/1‑101 (MQ=255)


                                   ttGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGc                                                                   >  2:2168147/1‑101 (MQ=255)


                                     gCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTc                                                                 <  1:837972/101‑1 (MQ=255)


                                     gCGGATTGTCACTCTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTc                                                                 <  2:59402/101‑1 (MQ=255)


                                     cgatcttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTc                                                                 <  2:334062/96‑1 (MQ=255)


                                      cGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTcc                                                                >  1:1895722/1‑101 (MQ=255)


                                          ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTcccccc                                                            <  1:2185123/101‑1 (MQ=255)


                                           tGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCcg                                                           >  1:4134243/1‑101 (MQ=255)


                                            gTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCcgc                                                          <  1:1694552/101‑1 (MQ=255)


                                            gTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCcgc                                                          <  2:909710/101‑1 (MQ=255)


                                                cTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCgggg                                                      >  1:2143582/1‑101 (MQ=255)


                                                 tttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCCCggggg                                                     >  1:624208/1‑101 (MQ=255)


                                                    cgcgGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCCCgggggggg                                                  >  1:4461223/1‑98 (MQ=255)


                                                    cgcgGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGt                                                  <  1:1197426/101‑1 (MQ=255)


                                                     tggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGt                                                  <  2:370319/91‑1 (MQ=2)


                                                       gtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                <  2:4441580/93‑1 (MQ=2)


                                                       gtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                <  2:926689/93‑1 (MQ=2)


                                                       ggggCGAAGCGTCGCTCCCCCCGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGa                                               <  2:4225753/101‑1 (MQ=255)


                                                        tgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGa                                               >  2:3658365/8‑101 (MQ=0)


                                                         gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                              <  2:4047124/95‑1 (MQ=2)


                                                          ccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  1:2379125/96‑1 (MQ=2)


                                                            gctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                           <  1:2432820/98‑1 (MQ=2)


                                                            gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          >  2:2534149/1‑101 (MQ=255)


                                                            gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          >  2:1451597/1‑101 (MQ=255)


                                                            gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          <  1:3899687/101‑1 (MQ=255)


                                                              tgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAGCCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTAGATCGaaa                                         >  2:2423521/2‑101 (MQ=2)


                                                              tgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                         >  2:1916127/2‑101 (MQ=2)


                                                               gcgcccccccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGGGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                       <  1:921802/93‑1 (MQ=255)


                                                               gCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                       >  2:2465410/1‑101 (MQ=255)


                                                                cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                      <  2:2028242/101‑1 (MQ=255)


                                                                cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                      <  1:745632/101‑1 (MQ=255)


                                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGACGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGt                                    >  2:4102582/1‑101 (MQ=255)


                                                                       ccccccGCGGGGGCGTGGGACGAAACATCTGCATGTAGTCGGGGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGa                               <  1:4722309/101‑1 (MQ=255)


                                                                       ccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGa                               <  1:2692802/101‑1 (MQ=255)


                                                                       ccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGa                               <  2:2277483/101‑1 (MQ=255)


                                                                       ccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGATCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGa                               >  2:2202959/1‑101 (MQ=255)


                                                                         ccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATc                             <  2:2753256/101‑1 (MQ=255)


                                                                          cccGCGGGGGGGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                            <  1:3749910/101‑1 (MQ=255)


                                                                             gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                         <  2:2848268/101‑1 (MQ=255)


                                                                             gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                         <  1:2287525/101‑1 (MQ=255)


                                                                             gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                         <  1:3921331/101‑1 (MQ=255)


                                                                              cgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGGTCCCCCCGCGGGTGCGTGGATCGAAACTGGTATGGATCTGgcg                        >  1:303260/1‑101 (MQ=255)


                                                                              cgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcg                        >  2:11217/1‑101 (MQ=255)


                                                                              cgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcg                        >  1:1083018/1‑101 (MQ=255)


                                                                                    cGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACg                  >  1:1103903/1‑101 (MQ=255)


                                                                                     gTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                 >  1:2239688/1‑101 (MQ=255)


                                                                                     gTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                 >  1:1524427/1‑101 (MQ=255)


                                                                                     gTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                 >  1:3340734/1‑101 (MQ=255)


                                                                                      tGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                >  1:3713010/1‑101 (MQ=255)


                                                                                       ggATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa               >  2:4600258/1‑101 (MQ=255)


                                                                                       ggATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa               >  1:2611984/1‑101 (MQ=255)


                                                                                        gATCGAAACATCCGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa              <  1:2834193/101‑1 (MQ=255)


                                                                                           ccaaaCCTCTGCGTGCAGGCCGCGG.GGAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc           <  2:3103709/99‑1 (MQ=25)


                                                                                           cGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc           >  2:859857/1‑101 (MQ=255)


                                                                                           cGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc           <  2:1895610/101‑1 (MQ=255)


                                                                                            gAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt          <  1:1245721/101‑1 (MQ=255)


                                                                                            gAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt          >  2:2998213/1‑101 (MQ=255)


                                                                                            gAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt          <  1:3679406/101‑1 (MQ=255)


                                                                                            gAAACATCTGCACGTAGGCGGCGG.GCAGTGCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt          <  2:1484410/101‑1 (MQ=255)


                                                                                             aaccATCCGCATGTGGTCGGCGG.GCAGTTGGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTg         <  2:1906405/98‑1 (MQ=37)


                                                                                             aaaCATCTGCATGTAGTCGGGGG.GCAGTCCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTg         <  1:1950469/101‑1 (MQ=255)


                                                                                             aaaCATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTg         >  1:3525496/1‑101 (MQ=255)


                                                                                             aaaCATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTg         >  2:1632464/1‑101 (MQ=255)


                                                                                             aaaCATCTGCATGTAGGCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTg         <  1:874158/101‑1 (MQ=255)


                                                                                              aaCATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgt        <  1:4085980/101‑1 (MQ=255)


                                                                                                 aTCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgt     >  1:2441259/1‑101 (MQ=255)


                                                                                                  tCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc    <  2:3573827/101‑1 (MQ=255)


                                                                                                   cTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGtcgtcg   >  2:1071785/1‑101 (MQ=255)


TCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGC  >  NC_014034/1395568‑1395768

Base quality scores:


ATCG

< 3 ≤


ATCG

< 17 ≤


ATCG

< 25 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG