Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,689 T→C 5.6% intergenic (+501/‑430) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,6890T→C 5.6% 22.0 ‑1.3 ‑0.5242intergenic (+501/‑430)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/9):  ref base (122/106):  total (127/115)
Fisher's exact test strand distribution p-value = 2.71e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.19e-01

Only 100 of 276 total aligned reads displayed.

GGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGAT  >  NC_014034/1395589‑1395789
                                                                                                     |                                                                                                     
 gATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTc                                                                                                      <  1:618053/101‑1 (MQ=255)
 gATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGCGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTc                                                                                                      >  2:4722053/1‑101 (MQ=255)
  aTCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCg                                                                                                     <  2:1682454/101‑1 (MQ=255)
  aTCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCg                                                                                                     <  1:4666545/101‑1 (MQ=255)
   tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGa                                                                                                    >  2:1788551/1‑101 (MQ=255)
   tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGa                                                                                                    >  1:6287/1‑101 (MQ=255)
   tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGa                                                                                                    >  1:1291487/1‑101 (MQ=255)
   tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGa                                                                                                    >  2:3511641/1‑101 (MQ=255)
    cGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.g                                                                                                  >  2:3277359/1‑101 (MQ=255)
    cGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.g                                                                                                  >  2:1268809/1‑101 (MQ=255)
      aaaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.Gcc                                                                                                >  2:412842/1‑101 (MQ=255)
       aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCg                                                                                               <  2:141606/101‑1 (MQ=255)
        accaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGc                                                                                              >  1:1049710/1‑101 (MQ=255)
         cccccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCa                                                                                             <  1:3054796/98‑1 (MQ=255)
         ccaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCa                                                                                             <  1:3181778/101‑1 (MQ=255)
          caccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAg                                                                                            >  2:2103009/1‑101 (MQ=255)
           cccTTGCGGATTGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGt                                                                                           <  2:1601340/100‑1 (MQ=255)
            ccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTc                                                                                          <  1:1999324/101‑1 (MQ=255)
             cTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCg                                                                                         >  1:2898617/1‑101 (MQ=255)
                cgatcttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTc                                                                                      <  2:334062/96‑1 (MQ=255)
                 cGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTcc                                                                                     <  1:1902272/101‑1 (MQ=255)
                   gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTTGCTcccc                                                                                   >  2:269110/1‑101 (MQ=255)
                       gTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCcgc                                                                               <  2:2914183/101‑1 (MQ=255)
                       gTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCcgc                                                                               <  1:1694552/101‑1 (MQ=255)
                       gTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCcgc                                                                               <  2:909710/101‑1 (MQ=255)
                           cTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCgggg                                                                           >  1:2143582/1‑101 (MQ=255)
                           cTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCgggg                                                                           <  2:3895058/101‑1 (MQ=255)
                            tttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCggggg                                                                          >  1:2277616/1‑101 (MQ=255)
                            tttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCggggg                                                                          >  2:1810897/1‑101 (MQ=255)
                               cgcgGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGt                                                                       >  2:227404/1‑101 (MQ=255)
                               cgcgGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGt                                                                       <  1:1197426/101‑1 (MQ=255)
                                tggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGt                                                                       <  2:370319/91‑1 (MQ=2)
                                tggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGt                                                                       <  1:100425/91‑1 (MQ=2)
                                  ggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                    <  1:2400245/101‑1 (MQ=255)
                                       gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                               >  2:2534149/1‑101 (MQ=255)
                                       gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCCCGGGGGCGTGGATCGaa                                                               >  2:3617230/1‑101 (MQ=255)
                                        nnngcgncccccccccGNGGGGGCGTGGATCAAAACATCTGCAGGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                              <  2:1903682/91‑1 (MQ=37)
                                          gcgcccccccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGGGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                            <  1:921802/93‑1 (MQ=255)
                                          gCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                            >  2:2465410/1‑101 (MQ=255)
                                           cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                           <  1:745632/101‑1 (MQ=255)
                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGt                                                         <  1:1221657/101‑1 (MQ=255)
                                             cccacgcccccGCGGGGGCGGGGATGGAAACATCTGCATGTGGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGt                                                         <  1:4345951/95‑1 (MQ=37)
                                               gccccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTAt                                                       <  2:1111510/98‑1 (MQ=255)
                                                 tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                                     >  1:2558229/1‑101 (MQ=255)
                                                 cccccccGGGGGGGCTTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                                     <  1:4133412/100‑1 (MQ=255)
                                                  ccccccGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGa                                                    <  2:2422947/101‑1 (MQ=255)
                                                  ccccccGCGGGGGCGTGGGACGAAACATCTGCATGTAGTCGGGGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGa                                                    <  1:4722309/101‑1 (MQ=255)
                                                  ccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGa                                                    <  1:2692802/101‑1 (MQ=255)
                                                  ccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGATCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGa                                                    >  2:2202959/1‑101 (MQ=255)
                                                    ccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATc                                                  <  1:2307920/101‑1 (MQ=255)
                                                     cccGCGGGGGGGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                                                 <  1:3749910/101‑1 (MQ=255)
                                                       cgcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTgg                                               <  2:4440136/101‑1 (MQ=255)
                                                        gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                                              <  2:2848268/101‑1 (MQ=255)
                                                        gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                                              <  1:3921331/101‑1 (MQ=255)
                                                         cgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGGTCCCCCCGCGGGTGCGTGGATCGAAACTGGTATGGATCTGgcg                                             >  1:303260/1‑101 (MQ=255)
                                                         cgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcg                                             >  1:1083018/1‑101 (MQ=255)
                                                         cgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcg                                             >  2:4469853/1‑101 (MQ=255)
                                                          gggggCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcgc                                            >  2:3981513/1‑101 (MQ=255)
                                                           ggggCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCa                                           >  1:4028952/1‑101 (MQ=255)
                                                           ggggCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTAGCGCa                                           <  1:3465406/101‑1 (MQ=255)
                                                               cGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACg                                       >  1:1103903/1‑101 (MQ=255)
                                                                gTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                                      >  1:2239688/1‑101 (MQ=255)
                                                                gTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                                      >  1:3340734/1‑101 (MQ=255)
                                                                gTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                                      >  1:1524427/1‑101 (MQ=255)
                                                                 tGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                                     >  1:3713010/1‑101 (MQ=255)
                                                                  ggATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                                    >  2:4600258/1‑101 (MQ=255)
                                                                   gaccGAACCATCTGCTTGCAGGCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa                                   <  2:1972757/98‑1 (MQ=37)
                                                                   gATCGAAACATCCGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa                                   <  1:2834193/101‑1 (MQ=255)
                                                                    aTCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAg                                  >  1:4450003/1‑101 (MQ=255)
                                                                      ccaaaCCTCTGCGTGCAGGCCGCGG.GGAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc                                <  2:3103709/99‑1 (MQ=25)
                                                                      cGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc                                <  1:3551149/101‑1 (MQ=255)
                                                                      cGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc                                >  2:859857/1‑101 (MQ=255)
                                                                      cGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCACTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc                                >  2:2971104/1‑101 (MQ=255)
                                                                       gAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt                               >  2:945078/1‑101 (MQ=255)
                                                                       gAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt                               <  1:3679406/101‑1 (MQ=255)
                                                                       gAAACATCTGCACGTAGGCGGCGG.GCAGTGCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt                               <  2:1484410/101‑1 (MQ=255)
                                                                        aaaCATCTGCATGTAGGCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTg                              <  1:874158/101‑1 (MQ=255)
                                                                         aaCATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgt                             <  1:4085980/101‑1 (MQ=255)
                                                                          aCATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtc                            <  1:2466967/101‑1 (MQ=255)
                                                                             tgtccATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc                         <  2:1049631/97‑1 (MQ=255)
                                                                             tcggCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc                         <  2:2327616/98‑1 (MQ=255)
                                                                               tGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGc                       <  2:4083736/101‑1 (MQ=255)
                                                                                gCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCt                      >  1:824685/1‑101 (MQ=255)
                                                                                 cATGTAGTCGGCTG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTc                     >  1:4575174/1‑101 (MQ=255)
                                                                                   tGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTccc                   >  2:680513/1‑101 (MQ=255)
                                                                                    gTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccc                  >  2:3359987/1‑101 (MQ=255)
                                                                                    gTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccc                  <  1:3277560/101‑1 (MQ=255)
                                                                                      aggcggcgg.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccccc                <  2:1527242/98‑1 (MQ=255)
                                                                                       gTCGGCGG.GCATTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcg               <  1:1788660/101‑1 (MQ=255)
                                                                                       gTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcg               <  2:2384527/101‑1 (MQ=255)
                                                                                        tCGGCGG.CCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcgc              <  1:163999/101‑1 (MQ=255)
                                                                                        gcggggg.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcgc              <  1:2443524/100‑1 (MQ=255)
                                                                                        cttccgatctaGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcg               <  2:3639809/91‑1 (MQ=255)
                                                                                         cggcgg.GCAGTGCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcgcg             <  1:945155/101‑1 (MQ=255)
                                                                                             gg.gCTGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCggggg         <  2:4460973/101‑1 (MQ=255)
                                                                                             gg.gCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCggggg         >  2:1097349/1‑101 (MQ=255)
                                                                                              g.gCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGc        <  1:4470103/101‑1 (MQ=255)
                                                                                              g.gCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGc        <  1:2168271/101‑1 (MQ=255)
                                                                                                   gTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTgg    <  2:6264/101‑1 (MQ=255)
                                                                                                     tCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGAt  >  1:1659338/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
GGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGAT  >  NC_014034/1395589‑1395789

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG