Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,866 C→T 19.0% intergenic (+678/‑253) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8660C→T19.0% 155.4 ‑1.2 ‑0.0299intergenic (+678/‑253)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (34/23):  ref base (125/117):  total (159/140)
Fisher's exact test strand distribution p-value = 3.04e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.98e-01

Only 100 of 327 total aligned reads displayed.

CGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGT  >  NC_014034/1395766‑1395966
                                                                                                      |                                                                                                            
cgCCCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgc                                                                                                              <  1:2727344/101‑1 (MQ=255)
 gCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgcg                                                                                                             >  2:420409/1‑101 (MQ=255)
 gCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgcg                                                                                                             >  1:1682939/1‑101 (MQ=255)
 ccTCCCCCCGCGGGGGCGTGGCTCGAAACAATGCGGCGCGGG..GCTA.TGGCTGACTGCCCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgcgt                                                                                                            <  2:2349851/100‑2 (MQ=2)
   tCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcg.cc                                                                                                          >  1:1673164/1‑101 (MQ=255)
    ccccccGCGGGGGCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGCTCGAACCTGcg.ccg                                                                                                         >  2:4282814/1‑101 (MQ=255)
       cccGCGGGGGCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.Ct                                                                                                     >  1:627157/1‑101 (MQ=255)
         cgcgGGGGCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTAt                                                                                                   <  2:4605929/101‑1 (MQ=255)
           cgGGGGCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCg                                                                                                 >  2:41301/1‑101 (MQ=255)
           cgGGGGCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCg                                                                                                 <  2:1688436/101‑1 (MQ=255)
           cgGGGGCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCg                                                                                                 >  1:3743411/1‑101 (MQ=255)
             ggggCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCa                                                                                               >  1:2243367/1‑101 (MQ=255)
              gggCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCAt                                                                                              >  2:2867094/1‑101 (MQ=255)
              gggCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCAt                                                                                              <  1:2421373/101‑1 (MQ=255)
                 cGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCg                                                                                           >  1:3143783/1‑101 (MQ=255)
                  gTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCgg                                                                                          >  1:3068584/1‑101 (MQ=255)
                  gTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCgg                                                                                          >  2:3892616/1‑101 (MQ=255)
                     gATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCg                                                                                    >  2:2830722/1‑101 (MQ=255)
                      aTCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGa                                                                                   <  1:1305538/101‑1 (MQ=255)
                       tCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAg                                                                                  <  2:4574921/101‑1 (MQ=255)
                       tCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAg                                                                                  <  1:1115833/101‑1 (MQ=255)
                       tCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAg                                                                                  <  2:214027/101‑1 (MQ=255)
                        cGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgc                                                                                 <  1:3094044/101‑1 (MQ=255)
                        cGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgc                                                                                 >  1:3564269/1‑101 (MQ=255)
                         gAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgcg                                                                                >  1:2987602/1‑101 (MQ=255)
                         gAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgcg                                                                                >  2:2499260/1‑101 (MQ=255)
                          aaaC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgcgc                                                                               >  2:1083415/1‑101 (MQ=255)
                          aaaC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgcgc                                                                               >  1:3229331/1‑101 (MQ=255)
                            aC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGcgcc.g                                                                            >  1:681798/1‑101 (MQ=255)
                            aC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGcgcc.g                                                                            <  2:3334322/101‑1 (MQ=255)
                               cTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCc.gcg                                                                          <  2:570155/101‑1 (MQ=255)
                                tGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.gcg.c                                                                        >  2:2054214/1‑101 (MQ=255)
                                    gcgTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTc                                                                   <  2:1943930/101‑1 (MQ=255)
                                     cgTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCg                                                                  >  2:2731048/1‑101 (MQ=255)
                                     cgTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCg                                                                  <  2:2929731/101‑1 (MQ=255)
                                      gTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGc                                                                 >  2:2560557/1‑101 (MQ=255)
                                       tGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCt                                                                >  1:2187064/1‑101 (MQ=255)
                                         aGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTcc                                                              >  1:4178247/1‑101 (MQ=255)
                                            tGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTccccc                                                           >  1:1590180/1‑101 (MQ=255)
                                             gCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTcccccc                                                          >  2:1331500/1‑101 (MQ=255)
                                             gCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTcccccc                                                          <  1:388804/101‑1 (MQ=255)
                                               cAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCcgc                                                        <  1:1571376/101‑1 (MQ=255)
                                                 gTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCgg                                                      >  2:1183303/1‑101 (MQ=255)
                                                  tGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCggg                                                     <  1:1822897/101‑1 (MQ=255)
                                                    aTAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCggggg                                                   >  1:410959/1‑101 (MQ=255)
                                                     tAGAC.GCCGTGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGc                                                  <  1:1456901/101‑1 (MQ=255)
                                                         c.GCCGTGGACGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTgg                                              <  2:670166/101‑1 (MQ=255)
                                                         c.GCCGTGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTgg                                              <  2:1634283/101‑1 (MQ=255)
                                                           ccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGa                                             >  1:2477875/10‑101 (MQ=9)
                                                            ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTg                                               >  1:926766/8‑101 (MQ=2)
                                                            ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTg                                               >  1:2635031/8‑101 (MQ=2)
                                                            caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGAt                                            <  2:3640321/93‑1 (MQ=9)
                                                            caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGAt                                            >  1:1051135/9‑101 (MQ=9)
                                                             cGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATc                                           <  1:2805819/101‑1 (MQ=255)
                                                              gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                          <  2:6282/101‑1 (MQ=255)
                                                              gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                          <  1:1083501/101‑1 (MQ=255)
                                                               cgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATTC.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                           <  1:451413/97‑1 (MQ=2)
                                                               cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                         <  1:1761965/96‑1 (MQ=9)
                                                                cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                           <  2:3799758/98‑1 (MQ=2)
                                                                 ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTTCCCCCGCGGGGGCGTGGATCg                                          <  1:3496942/99‑1 (MQ=0)
                                                                 ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                          >  2:187100/3‑101 (MQ=2)
                                                                 gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       <  2:3182962/101‑1 (MQ=255)
                                                                 gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       >  2:4505871/1‑101 (MQ=255)
                                                                 gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       <  2:1760687/101‑1 (MQ=255)
                                                                  atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaaa                                      >  1:1901382/3‑100 (MQ=9)
                                                                  atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                      >  2:10341/3‑101 (MQ=9)
                                                                   cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                     <  2:2244124/101‑1 (MQ=255)
                                                                    gTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCCCGGGGGCGTGGCTCGAAAcaa                                    >  1:836167/1‑99 (MQ=9)
                                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt                                   <  2:329580/101‑1 (MQ=9)
                                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                   >  1:1540437/1‑101 (MQ=255)
                                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                   >  1:2900858/1‑101 (MQ=255)
                                                                      cGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAtn                                  <  1:288399/101‑2 (MQ=9)
                                                                      cGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                  <  1:1163300/101‑1 (MQ=9)
                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                 >  2:729288/1‑101 (MQ=255)
                                                                         tCCCCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                               <  1:2763678/101‑2 (MQ=9)
                                                                         tCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.TTCGCTCCCCCCCCGGGGGCGTGGACCGAAACGATCCGt                               >  2:336464/1‑101 (MQ=38)
                                                                          ccccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                              >  2:792497/1‑101 (MQ=255)
                                                                          ccccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                              >  2:1794338/1‑101 (MQ=255)
                                                                          ccccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                              <  2:2424630/101‑1 (MQ=255)
                                                                          ccccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                              <  1:2143891/101‑1 (MQ=255)
                                                                          caccccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggc                              >  1:329750/3‑99 (MQ=9)
                                                                           cccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                             >  2:4665457/1‑101 (MQ=255)
                                                                            ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                            <  1:2560709/101‑1 (MQ=255)
                                                                             cccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                           >  1:3354545/1‑101 (MQ=255)
                                                                             cccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                           <  1:2939178/101‑1 (MQ=255)
                                                                             cccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                           <  1:4340982/101‑1 (MQ=255)
                                                                                 cgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcc                       >  2:54909/1‑92 (MQ=2)
                                                                                  gggggCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgggggcct                      >  1:1475855/1‑91 (MQ=2)
                                                                                  gggggCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                      <  2:473844/101‑11 (MQ=2)
                                                                                     ggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                   >  1:50225/1‑101 (MQ=255)
                                                                                          ggATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg              <  2:3051919/101‑1 (MQ=255)
                                                                                           gATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGc             <  2:3952884/101‑1 (MQ=255)
                                                                                           gATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGc             >  2:2655055/1‑101 (MQ=255)
                                                                                              cGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAgg          >  2:2156573/1‑100 (MQ=255)
                                                                                              cGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt          >  2:155039/1‑101 (MQ=255)
                                                                                               gAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg         >  1:3935720/1‑101 (MQ=255)
                                                                                                aaaCTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg        <  1:4665713/101‑1 (MQ=255)
                                                                                                    tGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg    <  1:2499404/101‑1 (MQ=255)
                                                                                                     gcg.cCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg   >  2:1259768/1‑101 (MQ=255)
                                                                                                      cg.ccgc.CTATCGCATCCGG...TCGAGGGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt  <  1:3892858/101‑1 (MQ=255)
                                                                                                      |                                                                                                            
CGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGT  >  NC_014034/1395766‑1395966

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG