Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,881 C→A 26.0% intergenic (+693/‑238) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8810C→A26.0% 211.8 ‑0.7 0.0294intergenic (+693/‑238)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (43/33):  ref base (112/103):  total (156/138)
Fisher's exact test strand distribution p-value = 5.08e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 311 total aligned reads displayed.

GGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGG  >  NC_014034/1395781‑1395981
                                                                                                    |                                                                                                        
 gCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATcc                                                                                                         <  2:846391/101‑1 (MQ=255)
  cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCg                                                                                                        <  1:49744/101‑1 (MQ=255)
  cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCg                                                                                                        <  1:4296719/101‑1 (MQ=255)
  cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCg                                                                                                        >  1:3143783/1‑101 (MQ=255)
   gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCgg                                                                                                       >  1:1144053/1‑101 (MQ=255)
   gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCgg                                                                                                       >  2:2143767/1‑101 (MQ=255)
   gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCgg                                                                                                       >  1:3068584/1‑101 (MQ=255)
   gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCgg                                                                                                       >  2:3892616/1‑101 (MQ=255)
    tGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...t                                                                                                   <  1:2568436/101‑1 (MQ=255)
     atctcttacccTGCGGCGTTAGTTGCAAGATAAAGGGCCCGCGGCCGTCGCTCCCCCCGCGGGGTCGGGGCTCGCACCTGCGCCGCCTATCGCATCCGG...Tc                                                                                                  <  2:710491/92‑1 (MQ=255)
      gATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCg                                                                                                 >  2:661501/1‑101 (MQ=255)
       aTCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGa                                                                                                <  1:962055/101‑1 (MQ=255)
       aTCGAAACCTGCGGCGTGAGTTGCCAATGATAGACGCCGGGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGa                                                                                                <  2:624144/101‑1 (MQ=255)
         cGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgc                                                                                              <  1:3094044/101‑1 (MQ=255)
          gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgcg                                                                                             >  1:2987602/1‑101 (MQ=255)
          gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgcg                                                                                             >  1:273862/1‑101 (MQ=255)
           aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgcgc                                                                                            >  1:3229331/1‑101 (MQ=255)
           aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgcgc                                                                                            <  1:2269927/101‑1 (MQ=255)
           aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgcgc                                                                                            >  2:1083415/1‑101 (MQ=255)
             aCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGcgccg                                                                                          <  2:3334322/101‑1 (MQ=255)
             aCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGcgccg                                                                                          >  1:3296950/1‑101 (MQ=255)
             aCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGcgccg                                                                                          >  1:1760795/1‑101 (MQ=255)
              ccTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGcgccgc                                                                                         <  2:2063090/101‑1 (MQ=255)
              ccTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGcgccgc                                                                                         >  2:1752005/1‑101 (MQ=255)
                  cggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.g                                                                                    >  2:1761693/1‑101 (MQ=255)
                    gcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTc                                                                                  <  2:1943930/101‑1 (MQ=255)
                      gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGc                                                                                >  2:2560557/1‑101 (MQ=255)
                       tGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCt                                                                               >  1:2187064/1‑101 (MQ=255)
                         aGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTcc                                                                             <  2:2838138/101‑1 (MQ=255)
                            tGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTccccc                                                                          <  1:1097435/101‑1 (MQ=255)
                            tGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTccccc                                                                          >  1:1590180/1‑101 (MQ=255)
                             gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTcccccc                                                                         >  2:1331500/1‑101 (MQ=255)
                               cAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCcgc                                                                       >  1:1462617/1‑101 (MQ=255)
                                 gTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCgg                                                                     >  2:1183303/1‑101 (MQ=255)
                                   gATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCgggg                                                                   <  1:2501861/101‑1 (MQ=255)
                                    aTAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCggggg                                                                  >  1:410959/1‑101 (MQ=255)
                                      aGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCg                                                                >  1:1884145/1‑101 (MQ=255)
                                        aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTg                                                              >  2:55719/1‑101 (MQ=255)
                                         cGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTgg                                                             <  2:3003468/101‑1 (MQ=255)
                                         cGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTgg                                                             <  1:1221323/101‑1 (MQ=255)
                                         cGCCGTGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTgg                                                             <  2:1634283/101‑1 (MQ=255)
                                          cggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGt                                                               <  2:1529940/93‑1 (MQ=2)
                                          ccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGa                                                            >  2:343678/10‑101 (MQ=9)
                                           ggaccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTg                                                              >  2:42853/8‑101 (MQ=2)
                                           ccGTGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                           <  2:74756/101‑1 (MQ=255)
                                           ccGTGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                           <  2:4526845/101‑1 (MQ=255)
                                           caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                           >  1:1051135/9‑101 (MQ=9)
                                           caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                           <  1:4191013/93‑1 (MQ=9)
                                            cGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  1:651309/101‑1 (MQ=255)
                                            aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                          >  2:1877404/8‑101 (MQ=9)
                                            aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                          >  2:4735758/8‑101 (MQ=9)
                                             cccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                            <  1:2900113/96‑1 (MQ=0)
                                             acctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         <  2:3151226/95‑1 (MQ=9)
                                               ggACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       >  1:1675084/1‑101 (MQ=255)
                                               ggACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       >  2:2368590/1‑101 (MQ=255)
                                               ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGGGGATCGaa                                                       >  2:2201040/5‑101 (MQ=9)
                                               ctaggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       <  1:2044672/97‑1 (MQ=9)
                                               cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  1:780312/98‑1 (MQ=2)
                                                ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         >  1:3326519/3‑101 (MQ=2)
                                                gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                      >  2:3862451/1‑101 (MQ=255)
                                                 gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                        <  1:575371/100‑1 (MQ=2)
                                                 atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                     >  2:10341/3‑101 (MQ=9)
                                                  cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                    <  2:2244124/101‑1 (MQ=255)
                                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                  >  1:1540437/1‑101 (MQ=255)
                                                       ccccccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCg                                               <  2:3261862/99‑1 (MQ=255)
                                                        tcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                              >  1:2485450/4‑100 (MQ=9)
                                                         ctccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggc                                             <  2:3694809/99‑3 (MQ=9)
                                                         ccccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                                             <  1:2143891/101‑1 (MQ=255)
                                                         ccccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                                             >  2:4581635/1‑101 (MQ=255)
                                                          cccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                                            >  2:4665457/1‑101 (MQ=255)
                                                            cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                          <  1:2939178/101‑1 (MQ=255)
                                                             ccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATg                                         <  1:1400466/101‑1 (MQ=255)
                                                              cgcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcggg                                        <  1:2806022/101‑8 (MQ=2)
                                                                cgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggagcgggcc                                      >  1:4384084/1‑92 (MQ=2)
                                                                cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                      >  1:1814253/1‑101 (MQ=255)
                                                                  ggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGATGGGTTCATGGCCTa                                    >  1:3547977/1‑101 (MQ=255)
                                                                   gggCGGGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                   <  2:2529445/101‑1 (MQ=255)
                                                                       gTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGGGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGGTCATGGCCTACGTCg                               >  1:851861/1‑101 (MQ=255)
                                                                         ggATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                             <  1:2690403/101‑1 (MQ=255)
                                                                          gATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGc                            <  2:3952884/101‑1 (MQ=255)
                                                                          gATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GCCGCTCCCCCCGCGGGGGCGTGGATTGAAACGATGCGATCATGGCCTACGTCGGGc                            >  1:286992/1‑101 (MQ=255)
                                                                           aTCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCa                           >  2:1824980/1‑101 (MQ=255)
                                                                             cGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                         >  2:2763740/1‑101 (MQ=255)
                                                                              gAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg                        <  1:2763904/101‑1 (MQ=255)
                                                                              gAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg                        >  2:4170018/1‑101 (MQ=255)
                                                                              gAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg                        >  1:3935720/1‑101 (MQ=255)
                                                                                 aCTGCGCCGCCTATCGCAGCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGAc                     <  2:2598178/101‑1 (MQ=255)
                                                                                  cTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACt                    <  2:3296748/101‑1 (MQ=255)
                                                                                  cTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACt                    <  1:1562980/101‑1 (MQ=255)
                                                                                     cgccgcCTATCGCATCCGG...TCGAGGGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt                 <  1:3892858/101‑1 (MQ=255)
                                                                                     cgccgcCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt                 <  2:2470484/101‑1 (MQ=255)
                                                                                     cgccgcCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt                 <  2:526242/101‑1 (MQ=255)
                                                                                      gccgccTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc                <  1:4556401/101‑1 (MQ=255)
                                                                                      gccgccTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc                <  2:1291395/101‑1 (MQ=255)
                                                                                       ccgccTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCg               <  2:3068396/101‑1 (MQ=255)
                                                                                         gccTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCt             <  2:4026845/101‑1 (MQ=255)
                                                                                           cTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcc           >  1:1173263/1‑101 (MQ=255)
                                                                                            tATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc          >  1:3409275/1‑101 (MQ=255)
                                                                                                    ccGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggg  <  1:3748608/101‑1 (MQ=255)
                                                                                                    ccGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggg  >  1:246076/1‑101 (MQ=255)
                                                                                                    |                                                                                                        
GGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGG  >  NC_014034/1395781‑1395981

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG