Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,936 G→T 3.5% intergenic (+748/‑183) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,9360G→T 3.5% 9.6 ‑2.0 ‑0.1202intergenic (+748/‑183)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/6):  ref base (89/106):  total (90/112)
Fisher's exact test strand distribution p-value = 1.34e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.55e-01

Only 100 of 235 total aligned reads displayed.

CTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGC  >  NC_014034/1395836‑1396036
                                                                                                     |                                                                                                     
 tcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                                                                                      >  1:1751061/4‑100 (MQ=9)
 tCCCCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                                                                                      <  1:2763678/101‑2 (MQ=9)
 tCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGCCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGt                                                                                                      >  2:1897407/1‑101 (MQ=255)
  ccccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                                                                                                     <  2:2424630/101‑1 (MQ=255)
  ccccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                                                                                                     <  1:2143891/101‑1 (MQ=255)
  caccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggc                                                                                                     >  1:329750/3‑99 (MQ=9)
   cccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                                                                                                    <  2:2597497/101‑1 (MQ=255)
    ccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgc                                                                                                   >  2:4385012/1‑97 (MQ=9)
    ccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                                                                                   <  1:2560709/101‑1 (MQ=255)
    ccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                                                                                   <  2:2851862/101‑1 (MQ=255)
     cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                                                                                  <  1:2939178/101‑1 (MQ=255)
     cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                                                                                  >  1:3354545/1‑101 (MQ=255)
     cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                                                                                  <  2:102659/101‑1 (MQ=255)
      ccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgg                                                                                                 <  2:4129461/101‑7 (MQ=2)
      ccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATg                                                                                                 <  1:1400466/101‑1 (MQ=255)
       cgcgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                                                                                                >  2:2328651/1‑101 (MQ=255)
       cgcgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                                                                                                <  2:4210594/101‑1 (MQ=255)
        gcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                                                                                               <  1:1674596/101‑9 (MQ=2)
         cgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcc                                                                                              >  2:3771056/1‑92 (MQ=2)
          gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgggggcct                                                                                             >  1:1475855/1‑91 (MQ=2)
          gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                                                                                             <  2:473844/101‑11 (MQ=2)
          gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGTGCGTTCATGGCCt                                                                                             >  1:1061814/1‑101 (MQ=255)
          gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                                                                             >  1:404846/1‑101 (MQ=255)
          gggggCGTGGATCGAAACTGCGCCGCCTAGCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                                                                             <  1:2409565/101‑1 (MQ=255)
            gggCGGGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                                                                           <  2:2529445/101‑1 (MQ=255)
            gggAGTGGATCGAACCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                                                                           <  1:324238/101‑1 (MQ=255)
             ggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                                                                                          <  2:681734/101‑1 (MQ=255)
              gCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                                                                                         >  1:86969/1‑101 (MQ=255)
               cGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                                                                                        >  2:2487879/1‑101 (MQ=255)
               cGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                                                                                        <  2:175458/101‑1 (MQ=255)
                  ggATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                                                                                     <  1:2690403/101‑1 (MQ=255)
                    aTCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCa                                                                                   <  2:3636679/101‑1 (MQ=255)
                      cGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAgg                                                                                 >  2:4015885/1‑100 (MQ=255)
                      cGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                                                                                 >  2:155039/1‑101 (MQ=255)
                      cGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                                                                                 >  2:2763740/1‑101 (MQ=255)
                       gAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg                                                                                >  1:3935720/1‑101 (MQ=255)
                         aaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGa                                                                              >  1:793757/1‑101 (MQ=255)
                           cTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACt                                                                            <  2:3296748/101‑1 (MQ=255)
                            tGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg                                                                           <  1:2499404/101‑1 (MQ=255)
                              cgccgcCTATCGCATCCGGTCGAGGGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt                                                                         <  1:3892858/101‑1 (MQ=255)
                              cgccgcCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt                                                                         <  2:526242/101‑1 (MQ=255)
                               gccgccTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc                                                                        <  2:1291395/101‑1 (MQ=255)
                                ccgccTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCg                                                                       <  1:4283061/101‑1 (MQ=255)
                                   ccTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTc                                                                    >  2:829270/1‑101 (MQ=255)
                                     tATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc                                                                  >  1:3409275/1‑101 (MQ=255)
                                     tATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc                                                                  >  2:1363095/1‑101 (MQ=255)
                                        cGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc                                                               <  1:1183393/101‑1 (MQ=255)
                                         gCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcg                                                              <  1:2867270/101‑1 (MQ=255)
                                           atacGGACGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGCTCACAGCCTACGTCGGGCAGCGGACTGGCCGGTCCCCCcgtg                                                            >  2:3918164/4‑99 (MQ=21)
                                             ccGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggg                                                          <  1:3748608/101‑1 (MQ=255)
                                               ggTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggggg                                                        <  1:3187939/101‑1 (MQ=255)
                                               ggTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGACGCTCCCCCCGCggggg                                                        >  1:4751006/1‑101 (MQ=255)
                                                gTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGTGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGc                                                       <  1:2324274/101‑1 (MQ=255)
                                                gTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGc                                                       <  2:627093/101‑1 (MQ=255)
                                                   gAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTg                                                   >  1:962550/1‑101 (MQ=255)
                                                      cgccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGAt                                                <  1:55726/101‑1 (MQ=255)
                                                       gccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATc                                               <  1:1259858/101‑1 (MQ=255)
                                                          gcgcA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGaa                                            <  2:410915/101‑1 (MQ=255)
                                                          caggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTCGACCGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGC.GTGGATCGa                                             <  2:1680610/95‑1 (MQ=2)
                                                           cgcA.GTCCCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGaaa                                           <  2:3354343/101‑1 (MQ=255)
                                                            ggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTCGACCGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGC.GTGGATCGaaa                                           <  1:2634268/97‑1 (MQ=2)
                                                            gcA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAAc                                          <  2:4365862/101‑1 (MQ=255)
                                                              cggTCGCGCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACt                                         <  1:456054/99‑1 (MQ=255)
                                                              a.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGGGGGATCGaaccc                                         >  1:686447/1‑98 (MQ=37)
                                                              a.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GGGGGTTGAAACtg                                        >  2:2284172/1‑100 (MQ=255)
                                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCg                                       >  1:4381871/1‑101 (MQ=255)
                                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGcc                                     >  2:107422/1‑101 (MQ=255)
                                                                     tCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTc                                  <  2:86953/101‑1 (MQ=255)
                                                                     cccccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTc                                  <  2:2701395/100‑1 (MQ=255)
                                                                      ccccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCg                                 <  2:1461568/101‑1 (MQ=255)
                                                                      ccccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGATCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCg                                 >  1:3548071/1‑101 (MQ=255)
                                                                       cccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGa                                <  2:3111619/101‑1 (MQ=255)
                                                                        ccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGAt                               >  2:758937/1‑101 (MQ=255)
                                                                         cccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATc                              <  2:1884033/101‑1 (MQ=255)
                                                                         cccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GGGGATCGAAACTCCCCCCCGATc                              >  1:847380/1‑101 (MQ=255)
                                                                          ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATcc                             <  1:4581888/101‑1 (MQ=255)
                                                                           cgcgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCcg                            >  2:388903/1‑101 (MQ=255)
                                                                             cgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCcgcg                          <  1:2999044/101‑1 (MQ=255)
                                                                             cgGGGGCGTGGATCGAAACGATGCGTTCAAGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCcgcg                          >  2:111859/1‑101 (MQ=255)
                                                                              gggggGGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGAC.GTGGATCGCAACTCGCCCTCGCTCcgcgg                         >  1:297851/1‑100 (MQ=38)
                                                                               ggggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCt                        <  1:2823714/101‑1 (MQ=255)
                                                                               ggggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCt                        <  1:1761801/101‑1 (MQ=255)
                                                                                gggGGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCtt                       <  2:4662317/101‑1 (MQ=255)
                                                                                gggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCtt                       <  2:3935478/101‑1 (MQ=255)
                                                                                 ggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTg                      >  1:4470961/1‑101 (MQ=255)
                                                                                    gTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCt                   >  2:1813916/1‑101 (MQ=255)
                                                                                    gTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCt                   >  1:2018074/1‑101 (MQ=255)
                                                                                      ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCa                 <  2:2987414/101‑1 (MQ=255)
                                                                                       gATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTTGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCAc                <  2:119661/101‑1 (MQ=255)
                                                                                         tCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGc              >  1:3537587/1‑101 (MQ=255)
                                                                                          cGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGcc             >  1:1088875/1‑101 (MQ=255)
                                                                                           gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCCCGCCt            >  1:2060740/1‑101 (MQ=255)
                                                                                           gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCt            >  2:3729097/1‑101 (MQ=255)
                                                                                           gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCt            >  1:2890823/1‑101 (MQ=255)
                                                                                            aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTg           >  2:3049513/1‑101 (MQ=255)
                                                                                              aCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGcc         <  2:3143583/101‑1 (MQ=255)
                                                                                               cGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCcg        >  1:3112429/1‑101 (MQ=255)
                                                                                               cGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCcg        <  1:1897519/101‑1 (MQ=255)
                                                                                                gATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCcgc       <  2:2906323/101‑1 (MQ=255)
                                                                                                     gTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGc  >  2:3216156/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
CTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGC  >  NC_014034/1395836‑1396036

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG