BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,938	T→G	3.4%	intergenic (+750/‑181)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,938	0	T→G	3.4%	8.5 ‑2.0 ‑0.0	200	intergenic (+750/‑181)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (1/6): ref base (88/105): total (89/111)
Fisher's exact test strand distribution p-value = 1.34e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.46e-01

Only 100 of 219 total aligned reads displayed.


CCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTC  >  NC_014034/1395838‑1396038


ccccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                                                                                                      <  2:2424630/101‑1 (MQ=255)


ccccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                                                                                                      >  2:792497/1‑101 (MQ=255)


ccccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                                                                                                      <  1:2143891/101‑1 (MQ=255)


 cccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                                                                                                     >  2:4665457/1‑101 (MQ=255)


 cccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                                                                                                     <  2:3143558/101‑1 (MQ=255)


 cccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                                                                                                     <  2:2597497/101‑1 (MQ=255)


  ccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                                                                                    <  1:2560709/101‑1 (MQ=255)


   cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                                                                                   >  1:3354545/1‑101 (MQ=255)


   cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                                                                                   <  1:3198916/101‑1 (MQ=255)


   cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                                                                                   <  2:102659/101‑1 (MQ=255)


    ccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATg                                                                                                  <  1:1400466/101‑1 (MQ=255)


     cgggggggCGTGGGTCGGAGCTGGGCCGCCTATCGCATCCGGTCGAGCGCCGGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                                                                                                 <  1:2965116/98‑1 (MQ=37)


       cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                                                                               <  2:2243243/101‑1 (MQ=255)


       cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                                                                               >  1:1814253/1‑101 (MQ=255)


        gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                                                                              >  2:177700/1‑101 (MQ=255)


        gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                                                                              >  1:2701549/1‑101 (MQ=255)


             cGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                                                                                         >  2:1702954/1‑101 (MQ=255)


              gTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGGTCATGGCCTACGTCg                                                                                        >  1:851861/1‑101 (MQ=255)


              gTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                                                                                        >  1:4027087/1‑101 (MQ=255)


                ggATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                                                                                      >  2:4646752/1‑101 (MQ=255)


                 gATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGc                                                                                     >  2:2655055/1‑101 (MQ=255)


                 gATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGc                                                                                     <  2:3952884/101‑1 (MQ=255)


                  aTCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCa                                                                                    <  2:3636679/101‑1 (MQ=255)


                   tCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAg                                                                                   <  2:1673059/101‑1 (MQ=255)


                    cGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAgg                                                                                  >  2:4015885/1‑100 (MQ=255)


                    cGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                                                                                  >  1:2906499/1‑101 (MQ=255)


                    cGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                                                                                  >  2:155039/1‑101 (MQ=255)


                      aaaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg                                                                                <  1:4665713/101‑1 (MQ=255)


                       aaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGa                                                                               <  2:273828/101‑1 (MQ=255)


                         cTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACt                                                                             <  1:1562980/101‑1 (MQ=255)


                         cTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACt                                                                             <  2:3296748/101‑1 (MQ=255)


                           gcgcCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg                                                                           >  2:1259768/1‑101 (MQ=255)


                            cgccgcCTATCGCATCCGGTCGAGGGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt                                                                          <  1:3892858/101‑1 (MQ=255)


                            cgccgcCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt                                                                          <  2:2470484/101‑1 (MQ=255)


                            cgccgcCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt                                                                          <  2:526242/101‑1 (MQ=255)


                              ccgccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCg                                                                        <  2:3068396/101‑1 (MQ=255)


                              ccgccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCg                                                                        <  1:4283061/101‑1 (MQ=255)


                                 ccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTc                                                                     >  2:829270/1‑101 (MQ=255)


                                  cTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcc                                                                    >  1:1173263/1‑101 (MQ=255)


                                   tATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCgagatc                                                                   >  1:3315069/1‑96 (MQ=255)


                                   tATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc                                                                   >  1:3409275/1‑101 (MQ=255)


                                      cGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc                                                                >  2:387858/1‑101 (MQ=255)


                                       gCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcg                                                               <  1:2867270/101‑1 (MQ=255)


                                       gCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGGCGCTCCCCCcg                                                               >  1:623188/1‑101 (MQ=255)


                                         atacGGACGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGCTCACAGCCTACGTCGGGCAGCGGACTGGCCGGTCCCCCcgtg                                                             >  2:3918164/4‑99 (MQ=21)


                                           ccGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggg                                                           >  1:246076/1‑101 (MQ=255)


                                           ccGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggg                                                           <  1:3748608/101‑1 (MQ=255)


                                             ggTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggggg                                                         >  1:3066500/1‑101 (MQ=255)


                                             ggTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGACGCTCCCCCCGCggggg                                                         >  1:4751006/1‑101 (MQ=255)


                                                 gAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTg                                                     >  2:1401359/1‑101 (MQ=255)


                                                 gAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTg                                                     >  1:962550/1‑101 (MQ=255)


                                                   gcgcCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGa                                                   >  1:1191625/1‑101 (MQ=255)


                                                   gcgcCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGa                                                   <  1:2998401/101‑1 (MQ=255)


                                                    cgccgcGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                  <  1:55726/101‑1 (MQ=255)


                                                     gccgcGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                 <  1:2092797/101‑1 (MQ=255)


                                                     gccgcGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                 <  1:1259858/101‑1 (MQ=255)


                                                        gcgcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                              <  1:2681727/101‑1 (MQ=255)


                                                        gcgcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                              <  2:410915/101‑1 (MQ=255)


                                                        aggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTCGACCGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                              <  2:4311944/96‑1 (MQ=2)


                                                         ggcAGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACGATGCTTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCGCCCCCCGCGGGGGCGTGGATCGaaa                                             <  1:2388687/100‑1 (MQ=255)


                                                         ggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTCGACCGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                             <  1:2634268/97‑1 (MQ=2)


                                                         cgcAGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                             <  2:3354343/101‑1 (MQ=255)


                                                          gatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTCGACCGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                            <  1:706452/98‑1 (MQ=2)


                                                          gatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTCGACCGAGGTCACGGCGGGCTCTCGTCGCTCCCCCCGCGGGGGCGGGGATCGaaaa                                            >  2:1319626/4‑100 (MQ=2)


                                                           cggTCGCGCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                           <  1:456054/99‑1 (MQ=255)


                                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCg                                         >  1:4381871/1‑101 (MQ=255)


                                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGc                                        >  2:4139755/1‑101 (MQ=255)


                                                                  tCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTc                                    <  2:86953/101‑1 (MQ=255)


                                                                  tCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTc                                    >  1:3254466/1‑101 (MQ=255)


                                                                   ccccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCg                                   <  1:1752110/101‑1 (MQ=255)


                                                                   ccccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGATCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCg                                   >  1:3548071/1‑101 (MQ=255)


                                                                      cccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATc                                <  2:1884033/101‑1 (MQ=255)


                                                                       ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATcc                               >  2:279867/1‑101 (MQ=255)


                                                                       ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATcc                               <  1:41308/101‑1 (MQ=255)


                                                                       ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATcc                               >  2:656849/1‑101 (MQ=255)


                                                                         gcgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCcgc                             >  2:750351/1‑101 (MQ=255)


                                                                          cgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTCCCTCGGTCcgcc                            >  1:815301/1‑100 (MQ=255)


                                                                          cgGGGGCGTGGATCGAAACGATGCGTTCAAGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCcgcg                            >  2:111859/1‑101 (MQ=255)


                                                                            ggggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCt                          <  1:2823714/101‑1 (MQ=255)


                                                                                 gTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCt                     >  1:2018074/1‑101 (MQ=255)


                                                                                 gTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCt                     >  2:1813916/1‑101 (MQ=255)


                                                                                  tGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc                    >  2:3736250/1‑101 (MQ=255)


                                                                                   ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCa                   <  2:2987414/101‑1 (MQ=255)


                                                                                   ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCa                   >  2:2510229/1‑101 (MQ=255)


                                                                                    gATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCAc                  <  2:4380455/101‑1 (MQ=255)


                                                                                     aTCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCAcc                 >  1:879937/1‑100 (MQ=255)


                                                                                      tCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTccctc                >  1:1084115/1‑97 (MQ=255)


                                                                                      tCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGc                >  1:3537587/1‑101 (MQ=255)


                                                                                         aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTg             >  2:3049513/1‑101 (MQ=255)


                                                                                            cGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCcg          >  1:3112429/1‑101 (MQ=255)


                                                                                            cGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCcg          <  1:1897519/101‑1 (MQ=255)


                                                                                             gATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCcgc         <  2:2906323/101‑1 (MQ=255)


                                                                                                gCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTc      <  2:2312661/101‑1 (MQ=255)


                                                                                                 cGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCg     <  2:1088789/101‑1 (MQ=255)


                                                                                                  gTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGc    >  2:3216156/1‑101 (MQ=255)


                                                                                                   ttCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCt   <  2:286958/101‑1 (MQ=255)


                                                                                                    tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTc  >  1:4516713/1‑101 (MQ=255)


                                                                                                    tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTc  >  1:3875343/1‑101 (MQ=255)


                                                                                                    tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTc  >  1:2293382/1‑101 (MQ=255)


                                                                                                    tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTc  >  1:100765/1‑101 (MQ=255)


CCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTC  >  NC_014034/1395838‑1396038

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG