Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,940 A→T 3.4% intergenic (+752/‑179) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,9400A→T 3.4% 9.1 ‑2.1 0.0205intergenic (+752/‑179)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/6):  ref base (96/102):  total (97/108)
Fisher's exact test strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

CCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCC  >  NC_014034/1395840‑1396040
                                                                                                    |                                                                                                     
ccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                                                                                       <  1:2560709/101‑1 (MQ=255)
ccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                                                                                       <  2:2851862/101‑1 (MQ=255)
 cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                                                                                      >  1:3354545/1‑101 (MQ=255)
 cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                                                                                      <  2:102659/101‑1 (MQ=255)
  ccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATg                                                                                                     <  1:1400466/101‑1 (MQ=255)
   cgggggggCGTGGGTCGGAGCTGGGCCGCCTATCGCATCCGGTCGAGCGCCGGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                                                                                                    <  1:2965116/98‑1 (MQ=37)
   cgcgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCGTTCATgg                                                                                                    >  1:1493179/1‑101 (MQ=255)
   cgcgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                                                                                                    <  2:4210594/101‑1 (MQ=255)
     cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                                                                                  <  2:2243243/101‑1 (MQ=255)
     cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                                                                                  >  1:1814253/1‑101 (MQ=255)
      gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                                                                                 >  1:2701549/1‑101 (MQ=255)
       ggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGATGGGTTCATGGCCTa                                                                                                >  1:3547977/1‑101 (MQ=255)
        gggCGGGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                                                                               <  2:2529445/101‑1 (MQ=255)
        gggAGTGGATCGAACCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                                                                               <  1:324238/101‑1 (MQ=255)
         ggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                                                                                              >  1:50225/1‑101 (MQ=255)
         ggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                                                                                              <  2:681734/101‑1 (MQ=255)
           cGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                                                                                            >  2:2487879/1‑101 (MQ=255)
           cGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                                                                                            <  2:175458/101‑1 (MQ=255)
            gTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                                                                                           >  1:4027087/1‑101 (MQ=255)
                  cGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                                                                                     >  2:2763740/1‑101 (MQ=255)
                  cGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                                                                                     >  1:2906499/1‑101 (MQ=255)
                    aaaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg                                                                                   <  1:3580874/101‑1 (MQ=255)
                      aCTGCGCCGCCTATCGCAGCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGAc                                                                                 <  2:2598178/101‑1 (MQ=255)
                        tGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg                                                                               <  1:2499404/101‑1 (MQ=255)
                         gcgcCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg                                                                              <  2:1590081/101‑1 (MQ=255)
                          cgccgcCTATCGCATCCGGTCGAGGGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt                                                                             <  1:3892858/101‑1 (MQ=255)
                          cgccgcCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt                                                                             <  2:526242/101‑1 (MQ=255)
                           gccgccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc                                                                            <  1:4556401/101‑1 (MQ=255)
                           gccgccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc                                                                            <  2:1291395/101‑1 (MQ=255)
                            gatcttATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCg                                                                           <  2:3314867/96‑1 (MQ=255)
                            ccgccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCg                                                                           <  1:4283061/101‑1 (MQ=255)
                            ccgccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCg                                                                           <  2:3068396/101‑1 (MQ=255)
                              gccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCt                                                                         <  2:4026845/101‑1 (MQ=255)
                               ccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTc                                                                        >  2:829270/1‑101 (MQ=255)
                                cTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcc                                                                       >  1:1173263/1‑101 (MQ=255)
                                 tATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCgagatc                                                                      >  1:3315069/1‑96 (MQ=255)
                                 tATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc                                                                      >  1:3409275/1‑101 (MQ=255)
                                    cGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc                                                                   >  2:3204659/1‑101 (MQ=255)
                                     gCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcg                                                                  <  1:2867270/101‑1 (MQ=255)
                                           ggTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggggg                                                            <  1:3187939/101‑1 (MQ=255)
                                           ggTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggggg                                                            >  1:3066500/1‑101 (MQ=255)
                                            gTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGc                                                           <  2:627093/101‑1 (MQ=255)
                                               gAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTg                                                       >  2:1401359/1‑101 (MQ=255)
                                               gAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTg                                                       >  1:962550/1‑101 (MQ=255)
                                                 gcgcCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGa                                                     <  1:2998401/101‑1 (MQ=255)
                                                 gcgcCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGa                                                     >  1:1191625/1‑101 (MQ=255)
                                                   gccgcGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATc                                                   <  1:1259858/101‑1 (MQ=255)
                                                     caggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTCGACCGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGC.GTGGATCGa                                                 <  2:1680610/95‑1 (MQ=2)
                                                      gcgcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGaa                                                <  2:410915/101‑1 (MQ=255)
                                                      gcgcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGaa                                                <  1:2681727/101‑1 (MQ=255)
                                                       ggcAGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACGATGCTTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCGCCCCCCGCGGGGGC.GTGGATCGaaa                                               <  1:2388687/100‑1 (MQ=255)
                                                       ggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTCGACCGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGC.GTGGATCGaaa                                               <  1:2634268/97‑1 (MQ=2)
                                                        gcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAAc                                              <  2:243343/101‑1 (MQ=255)
                                                        gcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAAc                                              <  2:4365862/101‑1 (MQ=255)
                                                          aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGGGGGATCGaaccc                                             >  1:686447/1‑98 (MQ=37)
                                                           gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCg                                           >  1:4381871/1‑101 (MQ=255)
                                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGc                                          >  2:4139755/1‑101 (MQ=255)
                                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GGGGATCGAAACTCGc                                          >  1:2131017/1‑101 (MQ=255)
                                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGcc                                         >  2:107422/1‑101 (MQ=255)
                                                               gtCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCt                                       <  2:1113036/100‑1 (MQ=255)
                                                                tCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGTCCTACGTCGGGCAGTGGACTGTTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTc                                      <  2:1493078/101‑1 (MQ=255)
                                                                tCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTc                                      <  2:86953/101‑1 (MQ=255)
                                                                cccccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTc                                      <  2:2701395/100‑1 (MQ=255)
                                                                  cccccGGGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGa                                    <  2:3238741/101‑1 (MQ=255)
                                                                   ccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGAt                                   >  2:758937/1‑101 (MQ=255)
                                                                    cccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATc                                  <  2:1884033/101‑1 (MQ=255)
                                                                     ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATcc                                 >  2:3860287/1‑101 (MQ=255)
                                                                     ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATcc                                 <  1:3862693/101‑1 (MQ=255)
                                                                     ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATcc                                 <  1:41308/101‑1 (MQ=255)
                                                                     ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATcc                                 <  1:4581888/101‑1 (MQ=255)
                                                                      cgcgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCcg                                >  2:388903/1‑101 (MQ=255)
                                                                      cgcgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCcg                                <  2:3550244/101‑1 (MQ=255)
                                                                        cgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCTCCCTCGGTCcgcc                              >  1:815301/1‑100 (MQ=255)
                                                                        cgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCcgcg                              <  1:729362/101‑1 (MQ=255)
                                                                        cgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCcgcg                              >  1:3500657/1‑101 (MQ=255)
                                                                          ggggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCt                            <  1:2823714/101‑1 (MQ=255)
                                                                           gggGGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCtt                           <  2:4662317/101‑1 (MQ=255)
                                                                           gggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCtt                           <  2:3935478/101‑1 (MQ=255)
                                                                           gggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCtt                           >  1:1745910/1‑101 (MQ=255)
                                                                            ggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTg                          >  1:4470961/1‑101 (MQ=255)
                                                                               gTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCt                       >  1:2018074/1‑101 (MQ=255)
                                                                               gTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCt                       >  2:1813916/1‑101 (MQ=255)
                                                                                 ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCa                     <  2:2987414/101‑1 (MQ=255)
                                                                                  gATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTTGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCAc                    <  2:119661/101‑1 (MQ=255)
                                                                                  gATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCAc                    >  2:3988324/1‑101 (MQ=255)
                                                                                   aTCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCAcc                   >  1:879937/1‑100 (MQ=255)
                                                                                    tCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTccctc                  >  1:1084115/1‑97 (MQ=255)
                                                                                      gAAACGATGCGTTCATGGGCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCt                <  1:4731998/101‑1 (MQ=255)
                                                                                      gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCCCGCCt                >  1:2060740/1‑101 (MQ=255)
                                                                                      gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCt                >  2:3729097/1‑101 (MQ=255)
                                                                                       aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTg               >  2:3049513/1‑101 (MQ=255)
                                                                                          cGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCcg            >  1:3112429/1‑101 (MQ=255)
                                                                                          cGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCcg            <  1:1897519/101‑1 (MQ=255)
                                                                                           gATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCcgc           <  2:2906323/101‑1 (MQ=255)
                                                                                              gCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTc        <  2:2312661/101‑1 (MQ=255)
                                                                                               cGTTCATGGCCTACGTCGGGCAGTGGACTGGTCCCCCCCCCCGCGGGGGC.GTGGATCGAACCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCg       <  2:2896890/101‑1 (MQ=255)
                                                                                                  tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTc    >  1:614023/1‑101 (MQ=255)
                                                                                                  tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTc    >  1:2293382/1‑101 (MQ=255)
                                                                                                  tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTc    >  1:100765/1‑101 (MQ=255)
                                                                                                   cATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTcc   >  1:859291/1‑101 (MQ=255)
                                                                                                    |                                                                                                     
CCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCC  >  NC_014034/1395840‑1396040

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG