BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,961	A→T	3.1%	intergenic (+773/‑158)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,961	0	A→T	3.1%	7.4 ‑3.5 ‑0.3	194	intergenic (+773/‑158)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (0/6): ref base (93/95): total (93/101)
Fisher's exact test strand distribution p-value = 2.96e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.09e-01

Only 100 of 210 total aligned reads displayed.


AACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1395861‑1396061


aaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGa                                                                                                       <  2:273828/101‑1 (MQ=255)


aaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGa                                                                                                       >  1:793757/1‑101 (MQ=255)


  cTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACt                                                                                                     <  1:1562980/101‑1 (MQ=255)


  cTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACt                                                                                                     <  2:3296748/101‑1 (MQ=255)


   tGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg                                                                                                    <  1:2499404/101‑1 (MQ=255)


    gcgcCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg                                                                                                   <  2:1590081/101‑1 (MQ=255)


    gcgcCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg                                                                                                   >  2:1259768/1‑101 (MQ=255)


    gcgcCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg                                                                                                   <  2:388949/101‑1 (MQ=255)


     cgccgcCTATCGCATCCGGTCGAGGGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt                                                                                                  <  1:3892858/101‑1 (MQ=255)


         gccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCt                                                                                              <  2:4026845/101‑1 (MQ=255)


          ccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTc                                                                                             <  1:420453/101‑1 (MQ=255)


           cTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcc                                                                                            >  1:1173263/1‑101 (MQ=255)


            tATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCgagatc                                                                                           >  1:3315069/1‑96 (MQ=255)


               cGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc                                                                                        >  2:2567574/1‑101 (MQ=255)


               cGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc                                                                                        >  2:387858/1‑101 (MQ=255)


                   tCCGGTCGAGCTCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCgg                                                                                    <  2:887438/101‑1 (MQ=255)


                    ccGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggg                                                                                   >  1:246076/1‑101 (MQ=255)


                      ggTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggggg                                                                                 >  1:3066500/1‑101 (MQ=255)


                      ggTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGACGCTCCCCCCGCggggg                                                                                 >  1:4751006/1‑101 (MQ=255)


                          gAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTg                                                                            >  2:1401359/1‑101 (MQ=255)


                            gcgcCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGa                                                                          >  1:1191625/1‑101 (MQ=255)


                            gcgcCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGa                                                                          <  1:2998401/101‑1 (MQ=255)


                             gaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTCGACCGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGC.GTGGAt                                                                         <  1:3163728/92‑1 (MQ=2)


                              gccgcGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATc                                                                        <  1:1259858/101‑1 (MQ=255)


                              gccgcGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATc                                                                        <  1:1844051/101‑1 (MQ=255)


                                 gcgcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGaa                                                                     <  1:2640973/101‑1 (MQ=255)


                                 gcgcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGaa                                                                     <  2:410915/101‑1 (MQ=255)


                                 aggatgTCGCGCCCCCCGCGGGGGCGTAGATCGAAACTATGCTGGCTCGACCGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGC.GTGGATCGaa                                                                     <  1:1994921/96‑1 (MQ=0)


                                  cgcAGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGaaa                                                                    <  2:3354343/101‑1 (MQ=255)


                                   gcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAAc                                                                   <  2:243343/101‑1 (MQ=255)


                                   gcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAAc                                                                   <  2:50218/101‑1 (MQ=255)


                                   gcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAAc                                                                   <  2:4365862/101‑1 (MQ=255)


                                   gatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTCGACCGAGGTCACGGCGGGCTCTCGTCGCTCCCCCCGCGGGGGC.GGGGATCGaaaa                                                                   >  2:1319626/4‑100 (MQ=2)


                                    cggTCGCGCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACt                                                                  <  1:456054/99‑1 (MQ=255)


                                     aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGGGGGATCGaaccc                                                                  >  1:686447/1‑98 (MQ=37)


                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCg                                                                >  1:4381871/1‑101 (MQ=255)


                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGc                                                               >  2:4139755/1‑101 (MQ=255)


                                          gtCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCt                                                            <  2:1113036/100‑1 (MQ=255)


                                           tCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGTCCTACGTCGGGCAGTGGACTGTTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTc                                                           <  2:1493078/101‑1 (MQ=255)


                                           tCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTc                                                           <  2:86953/101‑1 (MQ=255)


                                           tCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTc                                                           >  1:3254466/1‑101 (MQ=255)


                                           cccccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTc                                                           <  2:2701395/100‑1 (MQ=255)


                                            ccccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCg                                                          <  1:1752110/101‑1 (MQ=255)


                                            ccccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGATCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCg                                                          >  1:3548071/1‑101 (MQ=255)


                                               cccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATc                                                       <  2:1884033/101‑1 (MQ=255)


                                               cccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GGGGATCGAAACTCCCCCCCGATc                                                       >  1:847380/1‑101 (MQ=255)


                                                ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATcc                                                      <  1:4581888/101‑1 (MQ=255)


                                                  gcgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCcgc                                                    >  2:750351/1‑101 (MQ=255)


                                                   cgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCcgcg                                                   >  1:3500657/1‑101 (MQ=255)


                                                   cgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCcgcg                                                   <  1:729362/101‑1 (MQ=255)


                                                     ggggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCt                                                 <  1:2823714/101‑1 (MQ=255)


                                                     ggggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCt                                                 <  2:2900682/101‑1 (MQ=255)


                                                      gggGGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCtt                                                <  2:4662317/101‑1 (MQ=255)


                                                      gggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCtt                                                <  2:3935478/101‑1 (MQ=255)


                                                         cGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGcc                                             >  1:2173617/1‑101 (MQ=255)


                                                           tGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc                                           <  2:2986387/101‑1 (MQ=255)


                                                           tGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc                                           >  2:3736250/1‑101 (MQ=255)


                                                           tGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc                                           <  1:3406043/101‑1 (MQ=255)


                                                            ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCa                                          >  2:2510229/1‑101 (MQ=255)


                                                             gATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCAc                                         >  2:3988324/1‑101 (MQ=255)


                                                              aTCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACg                                        <  2:577179/101‑1 (MQ=255)


                                                               tCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTccctc                                       >  1:1084115/1‑97 (MQ=255)


                                                                cGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGcc                                      >  2:1004476/1‑101 (MQ=255)


                                                                 gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCCCGCCt                                     >  1:2060740/1‑101 (MQ=255)


                                                                 gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCt                                     >  2:800298/1‑101 (MQ=255)


                                                                 gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCt                                     >  1:2890823/1‑101 (MQ=255)


                                                                  aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTg                                    >  2:830817/1‑101 (MQ=255)


                                                                  aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTg                                    >  2:3049513/1‑101 (MQ=255)


                                                                    aCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGcc                                  <  2:3143583/101‑1 (MQ=255)


                                                                     cGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCcg                                 >  1:3112429/1‑101 (MQ=255)


                                                                          cGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCg                            <  2:1088789/101‑1 (MQ=255)


                                                                           gTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGc                           >  2:3216156/1‑101 (MQ=255)


                                                                            ttCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGCTCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCt                          <  2:286958/101‑1 (MQ=255)


                                                                             tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTc                         >  1:3875343/1‑101 (MQ=255)


                                                                             tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTc                         >  1:4516713/1‑101 (MQ=255)


                                                                             tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTc                         >  1:614023/1‑101 (MQ=255)


                                                                             tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTc                         >  1:2293382/1‑101 (MQ=255)


                                                                              cATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTcc                        >  1:859291/1‑101 (MQ=255)


                                                                                tGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTcccc                      >  2:2472438/1‑101 (MQ=255)


                                                                                   ccTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCcg                   <  2:4750750/101‑1 (MQ=255)


                                                                                     tACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCcgcg                 <  1:4636794/101‑1 (MQ=255)


                                                                                     tACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGCCGCTCCCCCcgcg                 >  2:3703753/1‑101 (MQ=255)


                                                                                      aCGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCgg                >  2:1053877/1‑101 (MQ=255)


                                                                                       cGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCggg               >  1:948638/1‑101 (MQ=255)


                                                                                       cGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCggg               >  2:1118716/1‑101 (MQ=255)


                                                                                       cGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GCGGATCGAAACTCGGCCTCGATCCGCGCGTGACTCACGCCTGCCGCCACGCTACCCCCGCggg               >  2:2284852/1‑101 (MQ=37)


                                                                                            ggCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCgg          >  1:2619908/1‑100 (MQ=255)


                                                                                             gCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACCCGCCCTCGATCCGCGCTTGCCTCACCCCTGCCGCGGCGCTCCCCCCGCggggggcgg         >  1:850513/1‑97 (MQ=38)


                                                                                               cgTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTGGa       <  1:4470887/100‑1 (MQ=255)


                                                                                               aGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTGGa       <  1:211065/101‑1 (MQ=255)


                                                                                               aGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTGGa       <  1:177725/101‑1 (MQ=255)


                                                                                                gTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTGTAt      <  2:3089516/101‑1 (MQ=255)


                                                                                                gTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTGGAt      <  2:644141/101‑1 (MQ=255)


                                                                                                gTGGACTGGTCGCCCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTGGAt      <  1:1004555/101‑1 (MQ=255)


                                                                                                 tGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTGGATc     <  2:2239564/101‑1 (MQ=255)


                                                                                                  ggACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTGGATCg    <  1:2328784/101‑1 (MQ=255)


                                                                                                  ggACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTGGATCg    <  1:829343/101‑1 (MQ=255)


                                                                                                  ggACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTGGATCg    >  2:2995570/1‑101 (MQ=255)


                                                                                                   gACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGCCGTGGATCGa   >  2:2153862/1‑101 (MQ=255)


                                                                                                    aCTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGATACTCGCCCTAGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa  <  1:4122714/101‑1 (MQ=255)


AACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1395861‑1396061

Base quality scores:


ATCG

< 3 ≤


ATCG

< 17 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG