Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,156 C→T 5.9% P13L (CCG→TTG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,1560C→T 5.9% 17.0 ‑1.1 ‑0.0170P13L (CCG→CTG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/7):  ref base (79/81):  total (82/88)
Fisher's exact test strand distribution p-value = 3.32e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.42e-01

Only 100 of 214 total aligned reads displayed.

TCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1396057‑1396256
                                                                                                     |                                                                                                     
 cGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgccgc                                                                                                     <  2:4620435/101‑1 (MQ=255)
 cGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgccgc                                                                                                     >  1:547076/1‑101 (MQ=255)
 cGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgccgc                                                                                                     >  2:344575/1‑101 (MQ=255)
 cGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgccgc                                                                                                     >  2:1630602/1‑101 (MQ=255)
  gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCgccgcc                                                                                                    <  1:2903733/101‑1 (MQ=255)
  gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCgccgcc                                                                                                    <  1:2079925/101‑1 (MQ=255)
  gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCgccccc                                                                                                    >  1:865183/1‑101 (MQ=255)
  gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCCCgccccc                                                                                                    >  1:1361085/1‑101 (MQ=255)
   aaaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCcgcgccc                                                                                                   >  1:1645038/1‑100 (MQ=255)
       aGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCGCt                                                                                               >  1:1500819/1‑101 (MQ=255)
       aGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCt                                                                                               <  2:4570156/101‑1 (MQ=255)
           gcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGGCACGCCGCgg                                                                                           >  2:1977547/1‑100 (MQ=255)
           gcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCGCTGCGt                                                                                           >  2:3408897/1‑101 (MQ=255)
             aTCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCg                                                                                         >  1:4125989/1‑101 (MQ=255)
             aTCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCg                                                                                         >  1:3289153/1‑101 (MQ=255)
              tCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGc                                                                                        <  1:1977664/101‑1 (MQ=255)
               cTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCCGCGTCGCt                                                                                       >  1:2969149/1‑101 (MQ=255)
                 gATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCGCTGCGTCGCTcc                                                                                     >  2:3043371/1‑101 (MQ=255)
                 gATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCGCTGCGTCGCCcc                                                                                     >  1:3136931/1‑101 (MQ=255)
                 gATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGGCGCTcc                                                                                     >  2:395632/1‑101 (MQ=255)
                  aTGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCGCTGCGTCGCTccc                                                                                    >  2:886399/1‑101 (MQ=255)
                  aTGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTccc                                                                                    >  1:652836/1‑101 (MQ=255)
                    gTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTccccc                                                                                  >  1:3165499/1‑101 (MQ=255)
                    gTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCCCTccccc                                                                                  >  2:1418765/1‑101 (MQ=255)
                    gTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGCCGCTccccc                                                                                  >  2:3862852/1‑101 (MQ=255)
                      atatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTccccccc                                                                                >  1:1824298/1‑100 (MQ=255)
                      atatGCCCGGTGAGACGGCTGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTccccccc                                                                                >  1:2694517/1‑100 (MQ=255)
                             cGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGc                                                                         >  2:2875943/1‑101 (MQ=255)
                             cGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGc                                                                         <  1:519238/101‑1 (MQ=255)
                             cGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGc                                                                         <  1:3389957/101‑1 (MQ=255)
                               gTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGCC.ATCTGG.CAGGCTCCCGCCGCACCGCCGCGCCTCTCCCCCCCCgggggggg                                                                       >  1:4708137/1‑98 (MQ=25)
                                tgtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCGCTG.GTCGCTCCCCCCGCGGGGGCGTg                                                                      <  1:3573061/91‑1 (MQ=9)
                                tgtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCGCTG.GTCGCTCCCCCCGCGGGGGCGTg                                                                      >  2:1886555/11‑101 (MQ=9)
                                 gtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCGCTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                     <  1:2742549/92‑1 (MQ=2)
                                 gtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCGCTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                     <  2:1355497/92‑1 (MQ=2)
                                 gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                     >  2:4071199/1‑101 (MQ=255)
                                 gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGGgg                                                                     >  1:3360423/1‑101 (MQ=255)
                                  agaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCCCGGGGGCGTGGa                                                                    >  2:2403416/1‑101 (MQ=255)
                                  aagcGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTTGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                    >  2:747629/4‑101 (MQ=255)
                                   gaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                   >  2:2966347/1‑101 (MQ=255)
                                   gaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                   <  2:1575601/101‑1 (MQ=255)
                                   ctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCCGCCTCGATCCGGTCAGCCACGTATTG.CGCGCTG.GTCGCTCACCCCGCGGGGGCGTGGAt                                                                   >  2:1545657/8‑101 (MQ=2)
                                      ggCTGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.a                                                               <  2:2588664/101‑1 (MQ=255)
                                       gCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.aa                                                              <  1:1540868/101‑1 (MQ=255)
                                       gCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.aa                                                              <  2:4125744/101‑1 (MQ=255)
                                         tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGGCTTCCTCCCCCCGCGGGGGGGTGGGTCG.AAAc                                                            >  2:2275799/1‑101 (MQ=37)
                                          gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAAcc                                                           <  1:1073624/101‑1 (MQ=255)
                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACTGTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCgg                                                        >  1:4222408/1‑101 (MQ=255)
                                               tCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCg                                                      <  1:40321/101‑1 (MQ=255)
                                               cccccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCg                                                      <  2:613959/100‑1 (MQ=255)
                                                ccccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGa                                                     <  2:2735335/101‑1 (MQ=255)
                                                ccccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGa                                                     >  2:2126557/1‑101 (MQ=255)
                                                ccccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGa                                                     >  1:2438056/1‑101 (MQ=255)
                                                 cccccGCAGGGTCGTGGATCGAACCTTTTCGTC.ATCTGG.CAGCCTGGCGCCAACCCGCTGCGTCCCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGaa                                                    <  2:297817/101‑1 (MQ=25)
                                                  ccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGaaa                                                   <  1:1159199/101‑1 (MQ=255)
                                                  ccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGaaa                                                   <  2:2636561/101‑1 (MQ=255)
                                                  ccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGaaa                                                   >  1:294540/1‑101 (MQ=255)
                                                   cccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGaaaa                                                  >  1:767829/1‑101 (MQ=255)
                                                    ccGCGGGGGCGTGGATCGAAAGTTTTCGTC.ATCTGG.CAGTCTCGCGCCGCCACGCTTCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGaaaaa                                                 <  1:3918406/101‑1 (MQ=255)
                                                     cgcgGGGGCGTGGATCGAAACTTTTCGTC.TTCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAAc                                                <  1:344609/101‑1 (MQ=255)
                                                        gggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGGGGATCG.AAACCCGGCGAAAAACGGc                                             >  2:1952544/1‑101 (MQ=255)
                                                           ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCAt                                          <  1:272742/101‑1 (MQ=255)
                                                           ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCAt                                          <  1:3049701/101‑1 (MQ=255)
                                                            gCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATg                                         <  2:3894336/101‑1 (MQ=255)
                                                            gCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATg                                         <  1:1068722/101‑1 (MQ=255)
                                                              gTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGcc                                       >  1:3659311/1‑101 (MQ=255)
                                                               tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCt                                      >  2:3682621/1‑101 (MQ=255)
                                                               tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCt                                      <  1:172893/101‑1 (MQ=255)
                                                                ggATCGAAACTTTTCGTC.ATCTGG.CAGGCCCGCGCCCCCCCGCCTGGGCCCTCCCCCCCCGGGGGCGGGGGTCGAAAACCCGGGGAAAAACGGcacggccc                                      >  2:3356747/1‑94 (MQ=255)
                                                                 gATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTcc                                    <  1:661565/101‑1 (MQ=255)
                                                                 gATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTcc                                    <  1:95491/101‑1 (MQ=255)
                                                                  aTCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCg                                   <  1:4388737/101‑1 (MQ=255)
                                                                   tCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCgg                                  <  1:2655209/101‑1 (MQ=255)
                                                                     gAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCgggg                                >  1:3305896/1‑100 (MQ=255)
                                                                     gAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGgt                                <  2:3204444/101‑1 (MQ=255)
                                                                      aaaCTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGgtg                               <  1:4475347/101‑1 (MQ=255)
                                                                        aCTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTc                             <  2:2060619/101‑1 (MQ=255)
                                                                           tttCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTgg                          >  2:4046769/1‑101 (MQ=255)
                                                                            ttCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGc                         >  1:4253012/1‑101 (MQ=255)
                                                                             tCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCt                        >  1:443185/1‑101 (MQ=255)
                                                                                tC.ATCTGG.CAGGCTCGCGCCGCCCCGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTc                     <  1:4715882/101‑1 (MQ=255)
                                                                                tC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTc                     >  2:476572/1‑101 (MQ=255)
                                                                                tC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTc                     <  2:2209958/101‑1 (MQ=255)
                                                                                 c.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCg                    <  2:443141/101‑1 (MQ=255)
                                                                                 c.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCg                    >  1:2788853/1‑101 (MQ=255)
                                                                                   aTCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGc                   >  2:3632692/1‑101 (MQ=255)
                                                                                      tGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTTTGGCTGtgcgtcc                >  2:3819811/1‑95 (MQ=255)
                                                                                      tGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcc                >  1:4354907/1‑101 (MQ=255)
                                                                                      tGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcc                >  2:1779089/1‑101 (MQ=255)
                                                                                      tGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcc                <  2:4356208/101‑1 (MQ=255)
                                                                                      tGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCGGTCGCTcc                >  1:1881722/1‑101 (MQ=255)
                                                                                       gg.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTccc               >  2:3798697/1‑101 (MQ=255)
                                                                                        g.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGGGTCTGGCTGTCGCTcccc              >  2:1289198/1‑101 (MQ=255)
                                                                                        g.CAGGCCCGCCCCGCCGCGGTGGCTGCCCCCCCCCGGGGGGGCGTGGATTG.AAACCCGGCGAAAAACCGGCAATCCCCCGGGTGTTCTGCTGTCGCCcccc              <  2:859217/101‑1 (MQ=255)
                                                                                          cAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTccccc             <  1:1311222/101‑1 (MQ=255)
                                                                                          cAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTccccc             <  2:3360221/101‑1 (MQ=255)
                                                                                           aGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcccccc            <  1:2203485/101‑1 (MQ=255)
                                                                                            ggCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCcg           <  2:3288951/101‑1 (MQ=255)
                                                                                              cTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCcgcg         >  2:1056702/1‑101 (MQ=255)
                                                                                                     cgccACGCTGCGTCGCTCCCCCCGCGTGGGCGTGGATCG.AAACCCCGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGt  <  1:3988566/101‑1 (MQ=255)
                                                                                                     |                                                                                                     
TCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1396057‑1396256

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG