BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,539	T→A	17.3%	C141S (TGC→AGC)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,539	0	T→A	17.3%	121.2 ‑0.3 ‑0.6	237	C141S (TGC→AGC)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (21/20): ref base (94/102): total (115/122)
Fisher's exact test strand distribution p-value = 7.34e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.54e-01

Only 100 of 253 total aligned reads displayed.


TCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCG  >  NC_014034/1396439‑1396637


tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                                                      >  1:4304808/1‑101 (MQ=255)


tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                                                      <  2:1959823/101‑1 (MQ=255)


 cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                                                                     >  1:4142198/1‑101 (MQ=255)


  gCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc                                                                                                    >  2:3424288/1‑101 (MQ=255)


     ccccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCttt                                                                                                 <  1:549552/101‑1 (MQ=255)


     ccccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCttt                                                                                                 >  2:2552683/1‑101 (MQ=255)


       ccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.g                                                                                              >  2:2491729/1‑101 (MQ=255)


        cccGCGGGGGGGTAGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.Gt                                                                                             <  2:2238937/101‑1 (MQ=255)


           gcgGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTggg                                                                                          <  2:3889900/101‑1 (MQ=255)


             gggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGAc                                                                                        >  1:3885040/1‑101 (MQ=255)


             gggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGAc                                                                                        >  2:3401460/1‑101 (MQ=255)


              ggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACt                                                                                       <  1:2842908/101‑1 (MQ=255)


              ggggCGTGGAGCGAAGCGGACCGGAAATTCCGGGGGAAACGGGGCGGCGAGCACGTCGGTCCCCCCGCGGGGGTGTGGATCGAACCTGCTTTC.GTGGGACt                                                                                       <  1:4234601/101‑1 (MQ=255)


               gggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTg                                                                                      <  2:1845477/101‑1 (MQ=255)


               gggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTg                                                                                      <  2:4377072/101‑1 (MQ=255)


               gggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTg                                                                                      >  1:2119050/1‑101 (MQ=255)


                 gCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGc                                                                                    >  1:4377469/1‑101 (MQ=255)


                   gTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCa                                                                                  >  1:1128263/1‑101 (MQ=255)


                   gTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GGGGGACTGGCCa                                                                                  >  1:817456/1‑101 (MQ=255)


                   gTGGAGCGAACAGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCa                                                                                  <  2:1976792/101‑1 (MQ=255)


                     ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgc                                                                                <  2:1613788/101‑1 (MQ=255)


                       aTCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgccg                                                                              >  2:3487577/1‑101 (MQ=255)


                       aTCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgccg                                                                              <  1:2991480/101‑1 (MQ=255)


                        tCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgccgc                                                                             <  1:3921295/101‑1 (MQ=255)


                          gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgccc                                                                           <  1:1695041/101‑1 (MQ=255)


                          gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgccc                                                                           <  1:4698079/101‑1 (MQ=255)


                           aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgcccg                                                                          <  1:4730697/101‑1 (MQ=255)


                           aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgccca                                                                          <  2:1447449/101‑2 (MQ=255)


                              cGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCgc.gc                                                                      >  1:4484740/1‑101 (MQ=255)


                               ggACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.Gcc                                                                     >  1:1075298/1‑101 (MQ=255)


                                gcccTGAAATGCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCa                                                                    <  2:3288971/99‑1 (MQ=255)


                                  ccTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAg                                                                  <  2:4346064/101‑1 (MQ=255)


                                   cTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGt                                                                 <  2:3315938/101‑1 (MQ=255)


                                     gAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCg                                                               <  1:1964323/101‑1 (MQ=255)


                                      aaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGc                                                              >  1:4524869/1‑101 (MQ=255)


                                      aaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGc                                                              >  1:3967974/1‑101 (MQ=255)


                                      aaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCCAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGc                                                              >  2:1626129/1‑101 (MQ=255)


                                       aaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCt                                                             >  1:3091879/1‑101 (MQ=255)


                                       aaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCt                                                             <  2:1112873/101‑1 (MQ=255)


                                              cgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCcg                                                      <  2:4149312/101‑1 (MQ=255)


                                                  aaaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCggg                                                  >  2:787651/1‑101 (MQ=255)


                                                     cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGGCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGc                                               >  1:1457655/1‑101 (MQ=255)


                                                       cggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCgg                                             >  1:1411631/1‑100 (MQ=255)


                                                        ggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTg                                            >  2:2920117/1‑101 (MQ=255)


                                                          ttgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTgg                                           >  1:975000/11‑101 (MQ=2)


                                                          ttgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTgg                                           >  1:101147/11‑101 (MQ=2)


                                                            gcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                        <  2:639142/101‑1 (MQ=255)


                                                            gcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                        <  1:2264677/101‑1 (MQ=255)


                                                             ggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGAtt                                        <  2:2210744/94‑2 (MQ=2)


                                                             ggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATc                                        <  2:1906440/94‑1 (MQ=2)


                                                             ggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATc                                        <  1:231114/94‑1 (MQ=2)


                                                              gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                       <  1:207701/95‑1 (MQ=2)


                                                               aGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                     >  2:2089329/1‑101 (MQ=255)


                                                                gCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                    <  1:3375505/101‑1 (MQ=255)


                                                                 ggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGaaa                                    >  2:3617482/4‑101 (MQ=2)


                                                                  aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                  >  2:4405713/1‑101 (MQ=255)


                                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgt                                <  1:3791935/101‑4 (MQ=2)


                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                              >  2:2350639/1‑101 (MQ=255)


                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                               >  1:3128219/1‑97 (MQ=2)


                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                               <  2:738342/101‑5 (MQ=2)


                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGAGCGAAActgtg                               >  1:2349984/1‑97 (MQ=2)


                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgc                              >  1:2815372/1‑96 (MQ=2)


                                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgcc                             <  1:3147616/101‑7 (MQ=2)


                                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgcc                             >  1:1993710/1‑95 (MQ=2)


                                                                         tCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                           >  2:4679072/1‑101 (MQ=255)


                                                                         tCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccg                            >  1:3528254/1‑94 (MQ=2)


                                                                         tCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCCCACCCCGCGGGGGCGTGGATCGAAActgtgccg                            <  2:534112/101‑8 (MQ=2)


                                                                          ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                          >  2:269856/1‑101 (MQ=255)


                                                                          ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                          <  1:766408/101‑1 (MQ=255)


                                                                          ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                          <  1:698653/101‑1 (MQ=255)


                                                                          ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                           >  1:317168/1‑93 (MQ=2)


                                                                          ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                           <  2:2581231/101‑9 (MQ=2)


                                                                           cccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                          <  2:4083441/101‑10 (MQ=2)


                                                                            ccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggtt                         <  1:2530141/101‑11 (MQ=2)


                                                                             cccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCt                       >  1:365350/1‑101 (MQ=255)


                                                                             cccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCt                       <  1:4355788/101‑1 (MQ=255)


                                                                              ccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCtt                      <  2:3942795/101‑1 (MQ=255)


                                                                               ggggggggCGGGAACCGAACCTGC.TTC.CTGGGGCCGGCCCGCCCCCCCCGGCCAAG.CCCCCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                    <  2:828152/98‑1 (MQ=12)


                                                                               cgcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                     >  2:134803/1‑101 (MQ=255)


                                                                                gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                    <  1:4415368/101‑1 (MQ=255)


                                                                                gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                    <  1:494988/101‑1 (MQ=255)


                                                                                  gggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacga                  >  1:877768/1‑101 (MQ=255)


                                                                                   ggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                 <  1:2765314/101‑1 (MQ=255)


                                                                                    gggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgacg                <  2:1226240/101‑1 (MQ=255)


                                                                                     ggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGc               >  1:4185937/1‑101 (MQ=255)


                                                                                      gCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGcc              <  2:1307871/101‑1 (MQ=255)


                                                                                       cGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCt             >  1:3242046/1‑101 (MQ=255)


                                                                                        gTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa            <  2:3198111/101‑1 (MQ=255)


                                                                                         tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt           >  1:1777791/1‑101 (MQ=255)


                                                                                          ggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc          <  1:1845305/101‑1 (MQ=255)


                                                                                           taTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGT.GCCAAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt         <  1:1484717/100‑1 (MQ=255)


                                                                                            aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg        <  2:4285274/101‑1 (MQ=255)


                                                                                            aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg        <  1:3484788/101‑1 (MQ=255)


                                                                                            aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg        >  2:375481/1‑101 (MQ=255)


                                                                                            aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg        >  2:222126/1‑101 (MQ=255)


                                                                                            aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACGATTTCGTCGATTACGACGCCAATCta        >  2:1484616/1‑100 (MQ=255)


                                                                                             tCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGa       <  1:3424495/101‑1 (MQ=255)


                                                                                             tCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGa       >  1:427577/1‑101 (MQ=255)


                                                                                                 aaCTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTc   >  1:1637674/1‑101 (MQ=255)


                                                                                                  aCTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCg  >  2:4500666/1‑101 (MQ=255)


TCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCG  >  NC_014034/1396439‑1396637

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG