BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,553	T→C	16.7%	T145T (ACT→AGC)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,553	0	T→C	16.7%	97.7 0.0 ‑0.1	245	T145T (ACT→ACC)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (21/20): ref base (102/102): total (123/122)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.89e-01

Only 100 of 257 total aligned reads displayed.


GGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCG  >  NC_014034/1396453‑1396653


 gggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTg                                                                                                      <  2:4377072/101‑1 (MQ=255)


 gggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTg                                                                                                      >  1:2119050/1‑101 (MQ=255)


    cGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGcc                                                                                                   <  1:1969332/101‑1 (MQ=255)


     gTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GGGGGACTGGCCa                                                                                                  >  1:817456/1‑101 (MQ=255)


       ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgc                                                                                                <  2:1613788/101‑1 (MQ=255)


         aTCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgccg                                                                                              >  2:3487577/1‑101 (MQ=255)


         aTCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgccg                                                                                              <  2:46093/101‑1 (MQ=255)


          tCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgccgc                                                                                             <  1:3921295/101‑1 (MQ=255)


            gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgccc                                                                                           >  1:2810464/1‑101 (MQ=255)


            gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgccc                                                                                           <  1:1695041/101‑1 (MQ=255)


             aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgcccg                                                                                          <  2:2741532/101‑1 (MQ=255)


             aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgccca                                                                                          <  2:1447449/101‑2 (MQ=255)


             aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTGC.GGGGGGCGGGCCAGccgcccg                                                                                          >  2:2255067/1‑101 (MQ=37)


                cGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCgc.gc                                                                                      >  2:1547293/1‑101 (MQ=255)


                 ggACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.Gcc                                                                                     >  1:1075298/1‑101 (MQ=255)


                      ttaaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTc                                                                                <  1:3268260/99‑1 (MQ=255)


                      tGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTc                                                                                <  1:2400568/101‑1 (MQ=255)


                       gAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCg                                                                               >  1:2347756/1‑101 (MQ=255)


                         aaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCt                                                                             <  1:4706497/101‑1 (MQ=255)


                          aTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTc                                                                            <  1:3884248/101‑1 (MQ=255)


                           ttCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTcc                                                                           <  1:4740297/101‑1 (MQ=255)


                           ttCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTcc                                                                           <  2:3266985/101‑1 (MQ=255)


                               gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTcccccc                                                                       >  1:2890930/1‑101 (MQ=255)


                               gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTcccccc                                                                       <  1:1574081/101‑1 (MQ=255)


                                cgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCcg                                                                      <  2:4149312/101‑1 (MQ=255)


                                 gggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCcgc                                                                     >  2:4436802/1‑101 (MQ=255)


                                  ggAAACGCGGCTGCGAGCACGTCGCTCCCCCCGCGGGGGCGTCGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCcgcg                                                                    <  2:1862296/101‑1 (MQ=255)


                                       cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCgggggg                                                               >  1:462736/1‑100 (MQ=255)


                                          ggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTg                                                            >  2:2920117/1‑101 (MQ=255)


                                           gcggcgAGCCCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                           <  2:4350521/101‑1 (MQ=255)


                                           gcggcgAGCACGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                           <  1:3975933/101‑1 (MQ=255)


                                            ttgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTgg                                                           <  1:4487620/91‑1 (MQ=2)


                                            ttgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTgg                                                           >  1:101147/11‑101 (MQ=2)


                                            cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                          <  2:1376610/101‑1 (MQ=255)


                                             tgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGa                                                          <  2:2030478/92‑1 (MQ=2)


                                             tgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGa                                                          >  2:3776056/10‑101 (MQ=2)


                                              gggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGAt                                                         <  1:3106783/93‑1 (MQ=2)


                                              gcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  2:639142/101‑1 (MQ=255)


                                              gcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  2:3744105/101‑1 (MQ=255)


                                               ggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGAtt                                                        <  2:2210744/94‑2 (MQ=2)


                                                gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                      >  1:1938492/1‑101 (MQ=255)


                                                gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGGGGATGGa                                                      >  2:4001342/1‑101 (MQ=255)


                                                 aGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     >  2:2089329/1‑101 (MQ=255)


                                                   cACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                   <  1:1775583/101‑1 (MQ=255)


                                                   cACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                   >  2:1930242/1‑101 (MQ=255)


                                                     agTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAAct                                                  <  2:2580116/100‑2 (MQ=2)


                                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                <  2:3438619/101‑1 (MQ=255)


                                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgt                                                >  1:4362526/1‑98 (MQ=2)


                                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgt                                                <  1:3791935/101‑4 (MQ=2)


                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                              >  2:2350639/1‑101 (MQ=255)


                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACTACTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                              >  1:4208268/1‑101 (MQ=255)


                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                                               <  2:738342/101‑5 (MQ=2)


                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                             <  1:1407013/101‑1 (MQ=255)


                                                         ccTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                             <  2:1128177/100‑1 (MQ=255)


                                                          cTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgcc                                             <  1:3147616/101‑7 (MQ=2)


                                                           tCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                           <  1:3487789/101‑1 (MQ=255)


                                                           tCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCCCACCCCGCGGGGGCGTGGATCGAAActgtgccg                                            <  2:534112/101‑8 (MQ=2)


                                                            ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                          <  1:766408/101‑1 (MQ=255)


                                                            ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                          <  1:698653/101‑1 (MQ=255)


                                                            ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                           >  2:791995/1‑93 (MQ=2)


                                                            ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                           >  1:317168/1‑93 (MQ=2)


                                                              ccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGc                                        >  1:2836835/1‑101 (MQ=255)


                                                               cccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCt                                       >  1:365350/1‑101 (MQ=255)


                                                                ccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCtt                                      >  1:4337394/1‑101 (MQ=255)


                                                                 ggggggggCGGGAACCGAACCTGC.TTC.CTGGGGCCGGCCCGCCCCCCCCGGCCAAG.CCCCCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                    <  2:828152/98‑1 (MQ=12)


                                                                 cgcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                                     <  1:862092/101‑1 (MQ=255)


                                                                  gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                    <  2:1075219/101‑1 (MQ=255)


                                                                  gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                    <  1:4415368/101‑1 (MQ=255)


                                                                  gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                    <  1:3586216/101‑1 (MQ=255)


                                                                     ggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                                 <  1:2765314/101‑1 (MQ=255)


                                                                      gggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgacg                                <  2:1226240/101‑1 (MQ=255)


                                                                        gCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGcc                              <  2:1307871/101‑1 (MQ=255)


                                                                          gTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                            <  2:3198111/101‑1 (MQ=255)


                                                                            ggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                          <  1:1845305/101‑1 (MQ=255)


                                                                             taTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGT.GCCAAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt                         <  1:1484717/100‑1 (MQ=255)


                                                                             gATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt                         >  1:2284601/1‑101 (MQ=255)


                                                                              aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                        <  2:4285274/101‑1 (MQ=255)


                                                                              aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                        >  1:498136/1‑101 (MQ=255)


                                                                              aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                        >  2:222126/1‑101 (MQ=255)


                                                                                cGAAACTGCTTTC.GGGGGACGGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAt                      <  2:25453/101‑1 (MQ=255)


                                                                                  aaaCTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                    <  2:3387410/101‑1 (MQ=255)


                                                                                    aCTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCg                  >  2:4500666/1‑101 (MQ=255)


                                                                                       gCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGt               <  2:181843/101‑1 (MQ=255)


                                                                                        cTTCC.GTGGGCCCGGCCGGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTc              <  1:2552835/101‑1 (MQ=38)


                                                                                         tttC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCg             >  2:4129507/1‑101 (MQ=255)


                                                                                         tttC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCg             >  1:2165919/1‑101 (MQ=255)


                                                                                          ttC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGc            >  1:3704816/1‑101 (MQ=255)


                                                                                          ttC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGc            >  1:2038085/1‑101 (MQ=255)


                                                                                            c.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc          >  2:3301388/1‑101 (MQ=255)


                                                                                            c.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc          <  1:1428878/101‑1 (MQ=255)


                                                                                            c.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc          <  2:2590146/101‑1 (MQ=255)


                                                                                            c.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc          >  2:4210230/1‑101 (MQ=255)


                                                                                            c.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc          <  1:2058527/101‑1 (MQ=255)


                                                                                              gggggACTGGCCAGCCGCCCGC.GCCAAGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc         <  1:3384570/99‑1 (MQ=255)


                                                                                              gTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGTTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc         >  1:4237642/1‑101 (MQ=255)


                                                                                              gTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc         <  1:3040210/101‑1 (MQ=255)


                                                                                                  gACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcccccc     >  1:4144252/1‑101 (MQ=255)


                                                                                                  gACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcccccc     >  1:968020/1‑101 (MQ=255)


                                                                                                    cTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgc   <  2:3509946/101‑1 (MQ=255)


                                                                                                     tGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgcg  >  1:3428629/1‑101 (MQ=255)


GGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCG  >  NC_014034/1396453‑1396653

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG