Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,561 C→G 18.4% P148R (CCG→CGG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,5610C→G18.4% 101.5 0.0 ‑0.1223P148R (CCG→CGG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (21/20):  ref base (94/88):  total (115/108)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.67e-01

Only 100 of 247 total aligned reads displayed.

ATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1396462‑1396661
                                                                                                    |                                                                                                     
   gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgccc                                                                                                   <  1:1695041/101‑1 (MQ=255)
    aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgcccg                                                                                                  <  2:2741532/101‑1 (MQ=255)
    aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgcccg                                                                                                  >  1:3767444/1‑101 (MQ=255)
    aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgccca                                                                                                  <  2:1447449/101‑2 (MQ=255)
    aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTGC.GGGGGGCGGGCCAGccgcccg                                                                                                  >  2:2255067/1‑101 (MQ=37)
        ggACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.Gcc                                                                                             >  1:1075298/1‑101 (MQ=255)
         gACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCa                                                                                            >  1:2055044/1‑101 (MQ=255)
         gACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCa                                                                                            >  1:303834/1‑101 (MQ=255)
            cTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGt                                                                                         >  2:806650/1‑101 (MQ=255)
             ttaaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTc                                                                                        <  1:3268260/99‑1 (MQ=255)
                aaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCt                                                                                     <  1:4706497/101‑1 (MQ=255)
                aaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCt                                                                                     >  1:3091879/1‑101 (MQ=255)
                  ttCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTcc                                                                                   <  1:4740297/101‑1 (MQ=255)
                  ttCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTcc                                                                                   <  2:3266985/101‑1 (MQ=255)
                   tCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTccc                                                                                  >  1:2859885/1‑101 (MQ=255)
                       cgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCcg                                                                              <  2:4149312/101‑1 (MQ=255)
                         ggAAACGCGGCTGCGAGCACGTCGCTCCCCCCGCGGGGGCGTCGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCcgcg                                                                            <  2:1862296/101‑1 (MQ=255)
                         ggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCcgcg                                                                            >  2:3467168/1‑101 (MQ=255)
                              cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGGCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGc                                                                       >  1:1457655/1‑101 (MQ=255)
                              cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCgggggg                                                                       >  1:462736/1‑100 (MQ=255)
                                cggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCgg                                                                     >  1:1411631/1‑100 (MQ=255)
                                 ggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTg                                                                    >  2:2920117/1‑101 (MQ=255)
                                  gcggcgAGCCCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                                   <  2:4350521/101‑1 (MQ=255)
                                   ttgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTgg                                                                   >  1:101147/11‑101 (MQ=2)
                                   ttgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTgg                                                                   <  1:4487620/91‑1 (MQ=2)
                                    tgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGa                                                                  <  2:2030478/92‑1 (MQ=2)
                                     gcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                <  1:2264677/101‑1 (MQ=255)
                                      ggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGAtt                                                                <  2:2210744/94‑2 (MQ=2)
                                       gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                                               <  1:207701/95‑1 (MQ=2)
                                       gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                              >  2:676732/1‑101 (MQ=255)
                                       gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                              >  1:1938492/1‑101 (MQ=255)
                                       gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGGGGATGGa                                                              >  2:4001342/1‑101 (MQ=255)
                                        acggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGa                                                              >  1:2323574/6‑101 (MQ=2)
                                        aGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                             >  2:2089329/1‑101 (MQ=255)
                                           aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCCCCCGC.GCCAAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACg                                                          <  1:3900883/101‑1 (MQ=255)
                                            agTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAAct                                                          <  2:2580116/100‑2 (MQ=2)
                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                        <  2:3438619/101‑1 (MQ=255)
                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgt                                                        >  1:4362526/1‑98 (MQ=2)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                                      >  1:2449429/1‑101 (MQ=255)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACTAC.ACC.GGGGCTCTGGGCACGGGTTGGG.ACGAAGGGGCTCCCCCCGCGGGGGCGTGGATCGaaagagcttt                                                     >  1:3932594/1‑94 (MQ=2)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                                                       <  2:738342/101‑5 (MQ=2)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGAGCGAAActgtg                                                       >  1:2349984/1‑97 (MQ=2)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgc                                                      >  1:2815372/1‑96 (MQ=2)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAAccgtgc                                                      >  2:4387854/1‑96 (MQ=2)
                                                ccTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                                     <  2:1128177/100‑1 (MQ=255)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgcc                                                     >  1:1993710/1‑95 (MQ=2)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgcc                                                     <  1:3147616/101‑7 (MQ=2)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                                   <  1:1547394/101‑1 (MQ=255)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                                   >  2:4679072/1‑101 (MQ=255)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCCCACCCCGCGGGGGCGTGGATCGAAActgtgccg                                                    <  2:534112/101‑8 (MQ=2)
                                                  cccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                                   <  2:1623476/100‑1 (MQ=255)
                                                   ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                                  <  1:698653/101‑1 (MQ=255)
                                                   ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                                  <  1:766408/101‑1 (MQ=255)
                                                   ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                                  <  2:2358878/101‑1 (MQ=255)
                                                   ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                                  >  1:3819252/1‑101 (MQ=255)
                                                   ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                                   >  1:317168/1‑93 (MQ=2)
                                                    cccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                                  <  2:3704306/101‑10 (MQ=2)
                                                       ccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCtt                                              >  2:3384364/1‑101 (MQ=255)
                                                        ggggggggCGGGAACCGAACCTGC.TTC.CTGGGGCCGGCCCGCCCCCCCCGGCCAAG.CCCCCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                            <  2:828152/98‑1 (MQ=12)
                                                        cgcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                                             >  2:134803/1‑101 (MQ=255)
                                                        cgcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                                             <  1:862092/101‑1 (MQ=255)
                                                         gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                            <  1:3586216/101‑1 (MQ=255)
                                                         gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                            <  2:1075219/101‑1 (MQ=255)
                                                         gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                            <  1:4415368/101‑1 (MQ=255)
                                                            ggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                                         <  1:2765314/101‑1 (MQ=255)
                                                             gggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgacg                                        <  2:1226240/101‑1 (MQ=255)
                                                              ggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGc                                       >  1:4185937/1‑101 (MQ=255)
                                                               gCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGcc                                      <  2:1307871/101‑1 (MQ=255)
                                                                 gTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                                    <  2:3198111/101‑1 (MQ=255)
                                                                  tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                   >  1:1777791/1‑101 (MQ=255)
                                                                   ggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                                  <  1:1845305/101‑1 (MQ=255)
                                                                     aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                                <  1:3484788/101‑1 (MQ=255)
                                                                     aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                                >  2:222126/1‑101 (MQ=255)
                                                                     aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                                <  2:4285274/101‑1 (MQ=255)
                                                                     aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                                >  1:498136/1‑101 (MQ=255)
                                                                     aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACGATTTCGTCGATTACGACGCCAATCta                                >  2:1484616/1‑100 (MQ=255)
                                                                      tCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGa                               >  1:427577/1‑101 (MQ=255)
                                                                      tCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGa                               <  2:3819017/101‑1 (MQ=255)
                                                                       cGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAt                              <  1:806723/101‑1 (MQ=255)
                                                                       cGAAACTGCTTTC.GGGGGACGGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAt                              <  2:25453/101‑1 (MQ=255)
                                                                        gAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAtt                             >  2:1918641/1‑101 (MQ=255)
                                                                        gAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAtt                             >  1:3649138/1‑101 (MQ=255)
                                                                          aaCTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTc                           >  1:1637674/1‑101 (MQ=255)
                                                                           aCTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCg                          >  2:4500666/1‑101 (MQ=255)
                                                                               cTTCC.GTGGGCCCGGCCGGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTc                      <  1:2552835/101‑1 (MQ=38)
                                                                                tttC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCg                     >  2:4129507/1‑101 (MQ=255)
                                                                                tttC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCg                     >  1:2165919/1‑101 (MQ=255)
                                                                                 ttC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGc                    >  1:3704816/1‑101 (MQ=255)
                                                                                   c.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc                  >  2:4210230/1‑101 (MQ=255)
                                                                                   c.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc                  >  2:3301388/1‑101 (MQ=255)
                                                                                   c.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc                  <  2:2590146/101‑1 (MQ=255)
                                                                                     gggggACTGGCCAGCCGCCCGC.GCCAAGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc                 <  1:3384570/99‑1 (MQ=255)
                                                                                     gTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGTTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc                 >  1:4237642/1‑101 (MQ=255)
                                                                                      tGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTccc                >  2:4483875/1‑101 (MQ=255)
                                                                                         gACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcccccc             >  1:968020/1‑101 (MQ=255)
                                                                                           cTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgc           <  2:3509946/101‑1 (MQ=255)
                                                                                            tGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgcg          >  1:3428629/1‑101 (MQ=255)
                                                                                             ggCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCgg         >  2:1664357/1‑101 (MQ=255)
                                                                                               ccAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACTCCTATCTGATTTCGGGTCGCTCCCCCCGCgggg       >  1:4067282/1‑101 (MQ=255)
                                                                                                cAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCggggg      >  2:3764794/1‑101 (MQ=255)
                                                                                                    |                                                                                                     
ATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1396462‑1396661

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG