Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,566 G→A 18.9% A150T (GCG→AAC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,5660G→A18.9% 110.7 0.0 ‑0.3217A150T (GCG→ACG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (21/20):  ref base (89/87):  total (110/107)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.94e-01

Only 100 of 244 total aligned reads displayed.

AAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1396466‑1396666
                                                                                                     |                                                                                                     
aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgcccg                                                                                                       <  2:2741532/101‑1 (MQ=255)
   cGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCgc.gc                                                                                                   >  1:4484740/1‑101 (MQ=255)
    ggACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.Gcc                                                                                                  >  1:1075298/1‑101 (MQ=255)
     gcccTGAAATGCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCa                                                                                                 <  2:3288971/99‑1 (MQ=255)
     gACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCa                                                                                                 <  2:1302441/101‑1 (MQ=255)
       ccTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAg                                                                                               <  2:4346064/101‑1 (MQ=255)
        cTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGt                                                                                              <  2:3315938/101‑1 (MQ=255)
        cTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGt                                                                                              >  2:2058406/1‑101 (MQ=255)
        cTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGt                                                                                              >  2:806650/1‑101 (MQ=255)
          gacaTTCCGCGGGAAACGCGGCGGCGAGCACGTCTCTCCCCCCGCGCGGGCGTGGATCCAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GAAAAGTcc                                                                                            >  2:1406919/4‑100 (MQ=255)
          gAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCg                                                                                            >  1:2347756/1‑101 (MQ=255)
           aaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGc                                                                                           >  1:4524869/1‑101 (MQ=255)
            aaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCt                                                                                          <  1:4706497/101‑1 (MQ=255)
            aaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCt                                                                                          <  2:1112873/101‑1 (MQ=255)
            aaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCt                                                                                          >  1:3091879/1‑101 (MQ=255)
              ttCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTcc                                                                                        <  1:4740297/101‑1 (MQ=255)
               tCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTccc                                                                                       <  2:3487900/101‑1 (MQ=255)
                  gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTcccccc                                                                                    <  2:2214384/101‑1 (MQ=255)
                  gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTcccccc                                                                                    >  1:2890930/1‑101 (MQ=255)
                   cgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCcg                                                                                   <  2:4149312/101‑1 (MQ=255)
                    gggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCcgc                                                                                  >  2:4436802/1‑101 (MQ=255)
                          cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGc                                                                            <  2:2090457/101‑1 (MQ=255)
                             ggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTg                                                                         >  2:2920117/1‑101 (MQ=255)
                             ggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTg                                                                         <  1:3899027/101‑1 (MQ=255)
                               ttgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTgg                                                                        >  1:101147/11‑101 (MQ=2)
                               ttgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTgg                                                                        >  1:1443280/11‑101 (MQ=2)
                                tgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGa                                                                       >  2:1334467/10‑101 (MQ=2)
                                tgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGa                                                                       >  2:3776056/10‑101 (MQ=2)
                                  ggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATc                                                                     <  2:1906440/94‑1 (MQ=2)
                                   gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                                                    <  1:207701/95‑1 (MQ=2)
                                   gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                   >  2:676732/1‑101 (MQ=255)
                                   gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGGGGATGGa                                                                   >  2:4001342/1‑101 (MQ=255)
                                     gCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                 <  1:3375505/101‑1 (MQ=255)
                                     cggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGaa                                                                  >  1:344115/5‑101 (MQ=2)
                                      cACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                >  2:1930242/1‑101 (MQ=255)
                                        agTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAAct                                                               <  2:2580116/100‑2 (MQ=2)
                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                             >  1:4406004/1‑101 (MQ=255)
                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgt                                                             <  1:3791935/101‑4 (MQ=2)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                                           >  1:2449429/1‑101 (MQ=255)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACTAC.ACC.GGGGCTCTGGGCACGGGTTGGG.ACGAAGGGGCTCCCCCCGCGGGGGCGTGGATCGaaagagcttt                                                          >  1:3932594/1‑94 (MQ=2)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                                                            >  1:3128219/1‑97 (MQ=2)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                                                            >  1:1685824/1‑97 (MQ=2)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                                                            <  2:738342/101‑5 (MQ=2)
                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                                          <  1:1407013/101‑1 (MQ=255)
                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAAccgtgc                                                           >  2:4387854/1‑96 (MQ=2)
                                             cTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgcc                                                          >  1:1993710/1‑95 (MQ=2)
                                              tCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                                        >  2:4679072/1‑101 (MQ=255)
                                              tCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccg                                                         >  1:3528254/1‑94 (MQ=2)
                                              tCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCCCACCCCGCGGGGGCGTGGATCGAAActgtgccg                                                         <  2:534112/101‑8 (MQ=2)
                                               ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                                       <  1:698653/101‑1 (MQ=255)
                                               ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                                       <  1:766408/101‑1 (MQ=255)
                                               ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                                        >  2:2236717/1‑93 (MQ=2)
                                               ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                                        >  1:317168/1‑93 (MQ=2)
                                                cccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                                       <  2:4083441/101‑10 (MQ=2)
                                                cccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                                       <  2:3704306/101‑10 (MQ=2)
                                                 ccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGc                                                     >  1:2836835/1‑101 (MQ=255)
                                                   ccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCtt                                                   >  1:4337394/1‑101 (MQ=255)
                                                    ggggggggCGGGAACCGAACCTGC.TTC.CTGGGGCCGGCCCGCCCCCCCCGGCCAAG.CCCCCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                                 <  2:828152/98‑1 (MQ=12)
                                                    cgcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGTTTCGGCGCTTa                                                  >  1:2135845/1‑101 (MQ=255)
                                                       gggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacga                                               >  1:877768/1‑101 (MQ=255)
                                                        ggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                                              <  1:2765314/101‑1 (MQ=255)
                                                          ggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGc                                            >  1:4185937/1‑101 (MQ=255)
                                                            cGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCt                                          >  1:3242046/1‑101 (MQ=255)
                                                             gTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                                         <  2:3198111/101‑1 (MQ=255)
                                                              tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                        >  1:1777791/1‑101 (MQ=255)
                                                              tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                        <  2:4484490/101‑1 (MQ=255)
                                                              tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                        <  2:2414158/101‑1 (MQ=255)
                                                               ggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                                       <  1:1845305/101‑1 (MQ=255)
                                                                taTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGT.GCCAAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt                                      <  1:1484717/100‑1 (MQ=255)
                                                                gATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt                                      >  1:2284601/1‑101 (MQ=255)
                                                                 aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                                     <  1:1930359/101‑1 (MQ=255)
                                                                 aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                                     <  2:4285274/101‑1 (MQ=255)
                                                                 aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                                     >  1:498136/1‑101 (MQ=255)
                                                                 aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                                     >  2:375481/1‑101 (MQ=255)
                                                                 aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACGATTTCGTCGATTACGACGCCAATCta                                     >  2:1484616/1‑100 (MQ=255)
                                                                  tCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGa                                    <  1:3424495/101‑1 (MQ=255)
                                                                   cGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAt                                   <  1:2232084/101‑1 (MQ=255)
                                                                   cGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAt                                   <  1:806723/101‑1 (MQ=255)
                                                                   cGAAACTGCTTTC.GGGGGACGGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAt                                   <  2:25453/101‑1 (MQ=255)
                                                                    gAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAtt                                  <  1:4267518/101‑1 (MQ=255)
                                                                     aaaCTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                                 <  2:3387410/101‑1 (MQ=255)
                                                                     aaaCTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                                 >  2:1596379/1‑101 (MQ=255)
                                                                           cTTCC.GTGGGCCCGGCCGGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTc                           <  1:2552835/101‑1 (MQ=38)
                                                                             ttC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGGCGCCTATCTGATTTCCGGTCGc                         >  2:1173546/1‑101 (MQ=255)
                                                                             ttC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGc                         >  2:2337593/1‑101 (MQ=255)
                                                                             ttC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGc                         >  1:2038085/1‑101 (MQ=255)
                                                                               c.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc                       >  1:3611417/1‑101 (MQ=255)
                                                                               c.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc                       <  2:2590146/101‑1 (MQ=255)
                                                                                 gTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc                      >  2:3443685/1‑101 (MQ=255)
                                                                                 gTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc                      >  1:3712343/1‑101 (MQ=255)
                                                                                     gACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcccccc                  >  1:968020/1‑101 (MQ=255)
                                                                                      aCTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcg                 >  1:3666551/1‑101 (MQ=255)
                                                                                           ccAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACTCCTATCTGATTTCGGGTCGCTCCCCCCGCgggg            >  1:4067282/1‑101 (MQ=255)
                                                                                             cgcccccCGC.GCCAAGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGc          <  2:4202868/100‑1 (MQ=255)
                                                                                             aGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGc          >  2:1734577/1‑101 (MQ=255)
                                                                                                cgcccgc.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTg       <  1:1596479/101‑1 (MQ=255)
                                                                                                 gcccgc.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTgg      >  2:1644783/1‑101 (MQ=255)
                                                                                                  cccgc.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGa     >  2:3258601/1‑101 (MQ=255)
                                                                                                  cccgc.GCCAAGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGa     <  1:2350772/101‑1 (MQ=255)
                                                                                                     gc.gcCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCg  <  2:1057681/101‑1 (MQ=255)
                                                                                                     |                                                                                                     
AAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1396466‑1396666

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG