Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,570 C→T 18.0% P151L (CCA→CTA)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,5700C→T18.0% 113.3 0.0 ‑0.1228P151L (CCA→CTA) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (21/20):  ref base (93/94):  total (114/114)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.56e-01

Only 100 of 247 total aligned reads displayed.

GGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1396470‑1396670
                                                                                                      |                                                                                                    
ggACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.Gcc                                                                                                      >  1:1075298/1‑101 (MQ=255)
 gACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCa                                                                                                     <  2:1302441/101‑1 (MQ=255)
    cTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGt                                                                                                  <  2:3315938/101‑1 (MQ=255)
    cTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGt                                                                                                  >  2:806650/1‑101 (MQ=255)
      gacaTTCCGCGGGAAACGCGGCGGCGAGCACGTCTCTCCCCCCGCGCGGGCGTGGATCCAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GAAAAGTcc                                                                                                >  2:1406919/4‑100 (MQ=255)
      gAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCg                                                                                                >  1:3163259/1‑101 (MQ=255)
      gAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCg                                                                                                <  1:1964323/101‑1 (MQ=255)
       aaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGc                                                                                               >  1:3967974/1‑101 (MQ=255)
       aaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGc                                                                                               >  1:4524869/1‑101 (MQ=255)
       aaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCCAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGc                                                                                               >  2:1626129/1‑101 (MQ=255)
        aaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCt                                                                                              <  1:4706497/101‑1 (MQ=255)
        aaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCt                                                                                              <  2:1112873/101‑1 (MQ=255)
          ttCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTcc                                                                                            <  2:3266985/101‑1 (MQ=255)
              gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTcccccc                                                                                        <  1:4754772/101‑1 (MQ=255)
              gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTcccccc                                                                                        >  1:2890930/1‑101 (MQ=255)
                      cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGc                                                                                <  2:2090457/101‑1 (MQ=255)
                          gcggcgAGCCCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                                            <  2:4350521/101‑1 (MQ=255)
                           ttgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTgg                                                                            >  1:975000/11‑101 (MQ=2)
                           ttgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTgg                                                                            >  1:101147/11‑101 (MQ=2)
                           ttgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTgg                                                                            >  1:1443280/11‑101 (MQ=2)
                            tgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGa                                                                           <  2:2030478/92‑1 (MQ=2)
                             gcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                         <  2:4304561/101‑1 (MQ=255)
                              ggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGAtt                                                                         <  2:2210744/94‑2 (MQ=2)
                               gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                       >  2:676732/1‑101 (MQ=255)
                                acggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGa                                                                       >  1:2323574/6‑101 (MQ=2)
                                 gCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                     <  1:3375505/101‑1 (MQ=255)
                                 cggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGaa                                                                      >  1:344115/5‑101 (MQ=2)
                                  ggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGaaa                                                                     >  2:329823/4‑101 (MQ=2)
                                   aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                                   >  2:4405713/1‑101 (MQ=255)
                                   aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCCCCCGC.GCCAAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACg                                                                   <  1:3900883/101‑1 (MQ=255)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                                               >  1:2449429/1‑101 (MQ=255)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACTACTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                                               >  1:4208268/1‑101 (MQ=255)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                                                                >  1:1685824/1‑97 (MQ=2)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                                                                <  2:738342/101‑5 (MQ=2)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                                                                >  1:3128219/1‑97 (MQ=2)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGAGCGAAActgtg                                                                >  1:2349984/1‑97 (MQ=2)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                                              <  1:1407013/101‑1 (MQ=255)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgc                                                               >  1:2815372/1‑96 (MQ=2)
                                          tCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccg                                                             >  1:3528254/1‑94 (MQ=2)
                                          tCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCCCACCCCGCGGGGGCGTGGATCGAAActgtgccg                                                             <  2:534112/101‑8 (MQ=2)
                                           ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                                           >  1:3819252/1‑101 (MQ=255)
                                           ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                                           >  2:269856/1‑101 (MQ=255)
                                           ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                                           <  1:766408/101‑1 (MQ=255)
                                           ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                                           <  2:2358878/101‑1 (MQ=255)
                                           ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                                            >  2:2236717/1‑93 (MQ=2)
                                            cccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                                           <  2:3704306/101‑10 (MQ=2)
                                             ccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAA.CCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggtt                                                          <  1:2530141/101‑11 (MQ=2)
                                              cccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCt                                                        <  1:4355788/101‑1 (MQ=255)
                                               ccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCtt                                                       <  2:3942795/101‑1 (MQ=255)
                                                ggggggggCGGGAACCGAACCTGC.TTC.CTGGGGCCGGCCCGCCCCCCCCGGCCAAG.CCCCCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                                     <  2:828152/98‑1 (MQ=12)
                                                cgcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                                                      >  2:134803/1‑101 (MQ=255)
                                                 gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                                     <  1:4415368/101‑1 (MQ=255)
                                                   gggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacga                                                   >  1:877768/1‑101 (MQ=255)
                                                    ggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                                                  >  2:3411328/1‑101 (MQ=255)
                                                    ggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                                                  <  1:2765314/101‑1 (MQ=255)
                                                     gggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgacg                                                 <  2:1226240/101‑1 (MQ=255)
                                                        cGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCt                                              >  1:3242046/1‑101 (MQ=255)
                                                          tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                            >  1:1777791/1‑101 (MQ=255)
                                                          tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                            <  2:3241845/101‑1 (MQ=255)
                                                           ggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                                           <  1:1845305/101‑1 (MQ=255)
                                                            taTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGT.GCCAAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt                                          <  1:1484717/100‑1 (MQ=255)
                                                            gATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt                                          >  1:2284601/1‑101 (MQ=255)
                                                             aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                                         <  1:1930359/101‑1 (MQ=255)
                                                              tCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGa                                        >  1:427577/1‑101 (MQ=255)
                                                              tCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGa                                        <  2:3819017/101‑1 (MQ=255)
                                                              tCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGa                                        <  1:3424495/101‑1 (MQ=255)
                                                               cGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAt                                       <  1:806723/101‑1 (MQ=255)
                                                               cGAAACTGCTTTC.GGGGGACGGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAt                                       <  2:25453/101‑1 (MQ=255)
                                                                gAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAtt                                      >  2:1918641/1‑101 (MQ=255)
                                                                 aaaCTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                                     >  2:1596379/1‑101 (MQ=255)
                                                                      gCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGt                                <  2:181843/101‑1 (MQ=255)
                                                                       cTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTc                               >  1:6219/1‑101 (MQ=255)
                                                                        tttC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCg                              >  2:4129507/1‑101 (MQ=255)
                                                                         ttC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGc                             >  2:2337593/1‑101 (MQ=255)
                                                                         ttC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGc                             >  1:2038085/1‑101 (MQ=255)
                                                                          tC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCt                            <  2:2577615/101‑1 (MQ=255)
                                                                           c.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc                           <  2:2590146/101‑1 (MQ=255)
                                                                           c.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc                           >  1:3611417/1‑101 (MQ=255)
                                                                           c.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc                           <  1:2058527/101‑1 (MQ=255)
                                                                           c.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc                           >  2:2942683/1‑101 (MQ=255)
                                                                           c.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc                           >  2:4210230/1‑101 (MQ=255)
                                                                             gTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc                          >  2:4166909/1‑101 (MQ=255)
                                                                             gTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc                          >  2:3443685/1‑101 (MQ=255)
                                                                             gTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc                          <  1:3040210/101‑1 (MQ=255)
                                                                             gTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc                          >  1:3712343/1‑101 (MQ=255)
                                                                              tGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTccc                         >  2:4483875/1‑101 (MQ=255)
                                                                               gggACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcccc                        <  2:4524616/101‑1 (MQ=255)
                                                                                 gACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcccccc                      >  1:4144252/1‑101 (MQ=255)
                                                                                  aCTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcg                     >  1:3666551/1‑101 (MQ=255)
                                                                                   cTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgc                    <  2:3509946/101‑1 (MQ=255)
                                                                                        cAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCggggg               >  2:3764794/1‑101 (MQ=255)
                                                                                         aGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGc              >  2:1734577/1‑101 (MQ=255)
                                                                                            cgcccgc.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTg           <  1:1596479/101‑1 (MQ=255)
                                                                                             gcccgc.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTgg          >  1:2144060/1‑101 (MQ=255)
                                                                                                  c.gcCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCCCGGGGGCGCGGATTGa     >  1:966617/1‑101 (MQ=255)
                                                                                                    gcCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa    <  2:2890754/101‑1 (MQ=255)
                                                                                                    gcCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa    <  2:1077495/101‑1 (MQ=255)
                                                                                                    gcCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa    <  1:2182642/101‑1 (MQ=255)
                                                                                                      cAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc  >  1:705093/1‑101 (MQ=255)
                                                                                                      cAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc  >  2:4076789/1‑101 (MQ=255)
                                                                                                      |                                                                                                    
GGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGC.GCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1396470‑1396670

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG