Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,572 A→T 17.8% S152C (AGT→TGT)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,5720A→T17.8% 121.0 0.0 ‑0.0230S152C (AGT→TGT) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (21/20):  ref base (95/94):  total (116/114)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.51e-01

Only 100 of 247 total aligned reads displayed.

CCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCA  >  NC_014034/1396473‑1396672
                                                                                                    |                                                                                                    
ccTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAg                                                                                                     <  2:4346064/101‑1 (MQ=255)
 cTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGt                                                                                                    >  2:2058406/1‑101 (MQ=255)
  tGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTc                                                                                                   <  1:2400568/101‑1 (MQ=255)
  tGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTc                                                                                                   <  2:4141952/101‑1 (MQ=255)
    aaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGc                                                                                                 >  1:3967974/1‑101 (MQ=255)
     aaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCt                                                                                                >  1:3091879/1‑101 (MQ=255)
     aaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCt                                                                                                <  2:1112873/101‑1 (MQ=255)
       ttCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcc                                                                                              <  1:4740297/101‑1 (MQ=255)
       ttCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcc                                                                                              <  2:3266985/101‑1 (MQ=255)
        tCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTccc                                                                                             >  1:2859885/1‑101 (MQ=255)
           gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccccc                                                                                          <  2:2214384/101‑1 (MQ=255)
            cgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcg                                                                                         <  2:2619374/101‑1 (MQ=255)
             gggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcgc                                                                                        >  2:4436802/1‑101 (MQ=255)
              ggAAACGCGGCTGCGAGCACGTCGCTCCCCCCGCGGGGGCGTCGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcgcg                                                                                       <  2:1862296/101‑1 (MQ=255)
              ggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcgcg                                                                                       >  2:3467168/1‑101 (MQ=255)
                aaaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCggg                                                                                     >  1:2577767/1‑101 (MQ=255)
                aaaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCggg                                                                                     >  2:787651/1‑101 (MQ=255)
                 aaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgggg                                                                                    <  2:2118929/101‑1 (MQ=255)
                   cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGGCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGc                                                                                  >  1:1457655/1‑101 (MQ=255)
                   cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgggggg                                                                                  >  1:462736/1‑100 (MQ=255)
                     cggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCgg                                                                                >  1:1411631/1‑100 (MQ=255)
                      ggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTg                                                                               <  1:3899027/101‑1 (MQ=255)
                       gcggcgAGCACGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                                              <  1:3975933/101‑1 (MQ=255)
                        ttgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTgg                                                                              >  1:975000/11‑101 (MQ=2)
                        ttgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTgg                                                                              <  1:4487620/91‑1 (MQ=2)
                        ttgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTgg                                                                              >  1:101147/11‑101 (MQ=2)
                        cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                             <  2:1376610/101‑1 (MQ=255)
                         tgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGa                                                                             >  2:3776056/10‑101 (MQ=2)
                          gggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGAt                                                                            <  2:4217148/93‑1 (MQ=2)
                          gcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                           <  2:3744105/101‑1 (MQ=255)
                          gcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                           <  1:2264677/101‑1 (MQ=255)
                           cgAGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                          <  2:727115/101‑1 (MQ=255)
                            gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                                                          <  2:4362906/95‑1 (MQ=2)
                            gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                         >  1:1938492/1‑101 (MQ=255)
                            gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGGGGATGGa                                                                         >  2:4001342/1‑101 (MQ=255)
                             acggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGa                                                                         >  1:2323574/6‑101 (MQ=2)
                               ggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGaaa                                                                       >  2:3617482/4‑101 (MQ=2)
                               cACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                      >  2:1930242/1‑101 (MQ=255)
                                 agTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAAct                                                                     <  2:2580116/100‑2 (MQ=2)
                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                                   <  2:3438619/101‑1 (MQ=255)
                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgt                                                                   >  1:4362526/1‑98 (MQ=2)
                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                                                 >  2:2350639/1‑101 (MQ=255)
                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                                                 >  1:727922/1‑101 (MQ=255)
                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                                                                  >  1:3128219/1‑97 (MQ=2)
                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGAGCGAAActgtg                                                                  >  1:2349984/1‑97 (MQ=2)
                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgc                                                                 >  1:2815372/1‑96 (MQ=2)
                                      ccccccccGCGGGGGCGTGGATCGAAGCTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcg                                                               <  2:1457561/99‑1 (MQ=255)
                                      cTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgcc                                                                >  1:1993710/1‑95 (MQ=2)
                                       tCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                                              >  2:4679072/1‑101 (MQ=255)
                                       tCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                                              <  1:1547394/101‑1 (MQ=255)
                                       tCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccg                                                               <  1:169587/101‑8 (MQ=2)
                                       tCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccg                                                               >  1:3528254/1‑94 (MQ=2)
                                       cccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                                              <  2:1623476/100‑1 (MQ=255)
                                        ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                                             >  1:3819252/1‑101 (MQ=255)
                                        ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                                             <  1:698653/101‑1 (MQ=255)
                                        ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                                              <  2:2565052/101‑9 (MQ=2)
                                        ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                                              >  1:317168/1‑93 (MQ=2)
                                         cccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                                             <  2:4083441/101‑10 (MQ=2)
                                          ccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggtt                                                            <  1:2530141/101‑11 (MQ=2)
                                           cccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCt                                                          >  1:365350/1‑101 (MQ=255)
                                            ccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCtt                                                         >  2:3384364/1‑101 (MQ=255)
                                            ccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCtt                                                         >  1:4337394/1‑101 (MQ=255)
                                             cgcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGTTTCGGCGCTTa                                                        >  1:2135845/1‑101 (MQ=255)
                                              gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                                       <  1:494988/101‑1 (MQ=255)
                                               cgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacg                                                      >  1:828319/1‑101 (MQ=255)
                                                  gggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgacg                                                   <  2:1226240/101‑1 (MQ=255)
                                                   ggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGc                                                  >  1:4185937/1‑101 (MQ=255)
                                                    gCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGcc                                                 <  2:1307871/101‑1 (MQ=255)
                                                     cGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCt                                                >  1:3242046/1‑101 (MQ=255)
                                                       tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                              <  2:4484490/101‑1 (MQ=255)
                                                       tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                              <  2:2414158/101‑1 (MQ=255)
                                                       tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                              <  2:3241845/101‑1 (MQ=255)
                                                          aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                                           <  2:617121/101‑1 (MQ=255)
                                                          aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                                           <  1:3484788/101‑1 (MQ=255)
                                                          aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                                           <  1:1789675/101‑1 (MQ=255)
                                                           tCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGa                                          <  1:3424495/101‑1 (MQ=255)
                                                               aaCTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTc                                      >  1:1637674/1‑101 (MQ=255)
                                                                        c.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc                             <  1:2058527/101‑1 (MQ=255)
                                                                        c.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc                             >  1:3611417/1‑101 (MQ=255)
                                                                        c.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc                             >  2:2942683/1‑101 (MQ=255)
                                                                        c.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc                             >  2:3301388/1‑101 (MQ=255)
                                                                          gggggACTGGCCAGCCGCCCGCGCCAAGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc                            <  1:3384570/99‑1 (MQ=255)
                                                                          gTGGGACTGGCCAGCCGCCCGCGCCAAGTCGTTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc                            >  1:4237642/1‑101 (MQ=255)
                                                                          gTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc                            >  1:3712343/1‑101 (MQ=255)
                                                                          gTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc                            >  1:802945/1‑101 (MQ=255)
                                                                            gggACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcccc                          >  1:1893249/1‑101 (MQ=255)
                                                                              ggcTGGCCAGCCGCCCGCGCCAAGTCGCGCCCCCCGCGGGGGGTTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcccccc                        <  2:825951/99‑1 (MQ=255)
                                                                                      cgcccccCGCGCCAAGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGc                <  2:4202868/100‑1 (MQ=255)
                                                                                          gcccgcGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTgg            >  2:1644783/1‑101 (MQ=255)
                                                                                            ccgcGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGAt          >  1:1235784/1‑101 (MQ=255)
                                                                                               cgcCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCCCGGGGGCGCGGATTGa       >  1:966617/1‑101 (MQ=255)
                                                                                                tctaaGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa      <  2:928875/98‑1 (MQ=255)
                                                                                                gcCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa      <  2:2890754/101‑1 (MQ=255)
                                                                                                gcCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa      >  2:1791930/1‑101 (MQ=255)
                                                                                                gcCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa      <  2:1077495/101‑1 (MQ=255)
                                                                                                gcCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa      <  1:2182642/101‑1 (MQ=255)
                                                                                                 ccAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa     >  2:3867961/1‑101 (MQ=255)
                                                                                                  cAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc    >  2:2245811/1‑101 (MQ=255)
                                                                                                   aaGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaaga   >  1:928952/1‑99 (MQ=255)
                                                                                                    aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCa  >  2:2746447/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
CCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCA  >  NC_014034/1396473‑1396672

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG