Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,816 A→T 13.6% H233L (CAT→CTG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,8160A→T13.6% 104.4 0.0 ‑0.0279H233L (CAT→CTT) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (20/18):  ref base (129/112):  total (149/130)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.95e-01

Only 100 of 296 total aligned reads displayed.

CCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGC  >  NC_014034/1396716‑1396916
                                                                                                       |                                                                                                    
ccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCa                                                                                                      <  2:3163059/101‑1 (MQ=255)
 cgcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCAt                                                                                                     >  1:3931307/1‑101 (MQ=255)
  gcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATg                                                                                                    <  2:427533/101‑1 (MQ=255)
  gcgGGGGCGTGGATCGAAACCCTGTCCCTCAGATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGAACGAAACCGTGTGGAT...GATCATg                                                                                                    >  1:4206341/1‑101 (MQ=255)
   cgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGc                                                                                                   <  1:1673205/101‑1 (MQ=255)
    gggggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCa                                                                                                  <  1:1063439/101‑1 (MQ=255)
     ggggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAg                                                                                                 >  1:1480035/1‑101 (MQ=255)
         cGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGcgcg                                                                                             <  2:3244835/101‑1 (MQ=255)
         cGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGGGGAT...GATCATGCAGcgcg                                                                                             >  2:587927/1‑101 (MQ=255)
         cGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGTGTGGAA...GATCATGCAGcacg                                                                                             >  2:4712482/1‑101 (MQ=255)
          gTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGTT...GATCATGCAGCGCgg                                                                                            >  2:3387257/1‑101 (MQ=255)
          gTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCgg                                                                                            >  2:2403500/1‑101 (MQ=255)
          gTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCgg                                                                                            <  1:757603/101‑1 (MQ=255)
                cGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCt                                                                                      >  2:4672201/1‑101 (MQ=255)
                 gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCtt                                                                                     >  2:1363078/1‑101 (MQ=255)
                 gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCtt                                                                                     <  1:318019/101‑1 (MQ=255)
                 gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCtt                                                                                     <  1:871900/101‑1 (MQ=255)
                  aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTg                                                                                    >  1:1191915/1‑101 (MQ=255)
                  aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTg                                                                                    >  2:1635022/1‑101 (MQ=255)
                      ccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTc                                                                                >  2:800414/1‑101 (MQ=255)
                        tGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCATCGCGGACGGCTTGGGTCGc                                                                              >  1:422634/1‑101 (MQ=255)
                         gTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCt                                                                             <  2:2152372/101‑1 (MQ=255)
                         gTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCt                                                                             >  2:1242778/1‑101 (MQ=255)
                           cccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGGTTGGGTCGCTcc                                                                           >  1:686665/1‑101 (MQ=255)
                            ccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTccc                                                                          >  2:76614/1‑101 (MQ=255)
                            ccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTccc                                                                          >  2:3641176/1‑101 (MQ=255)
                            ccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGAACGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTccc                                                                          <  2:4206095/101‑1 (MQ=255)
                             cTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTcccc                                                                         >  2:857373/1‑101 (MQ=255)
                              tCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTccccc                                                                        <  2:2836670/101‑1 (MQ=255)
                                atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCcg                                                                      <  2:4450054/101‑1 (MQ=255)
                                 tatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGGCGCTCCCCCcgc                                                                     >  1:3512848/1‑101 (MQ=255)
                                   tgctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGCTCGCTCCCCCCGCgg                                                                   <  1:4001584/101‑1 (MQ=255)
                                     ctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCgggg                                                                 <  2:4144006/101‑1 (MQ=255)
                                     ctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCgggg                                                                 >  1:2246813/1‑101 (MQ=255)
                                         cACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCgg                                                             >  1:3438432/1‑100 (MQ=255)
                                           cGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTgg                                                           <  1:1346858/101‑1 (MQ=255)
                                            gATGCCCTTGAGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGa                                                          <  2:2002446/101‑1 (MQ=255)
                                             tcacatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                           >  1:3462855/11‑101 (MQ=2)
                                             tcacatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                           <  1:1099666/91‑1 (MQ=2)
                                             aTGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         >  1:1640768/1‑101 (MQ=255)
                                              tccatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                          <  2:1373718/92‑1 (MQ=0)
                                              tGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  1:1877014/101‑1 (MQ=255)
                                              tGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  2:3931065/101‑1 (MQ=255)
                                               gCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                       <  2:913741/101‑1 (MQ=255)
                                               gCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCCCCCCCCGCGGGGGCGTGGATCg                                                       <  1:942372/101‑1 (MQ=255)
                                                 ccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     <  1:587978/101‑1 (MQ=255)
                                                  ctggAGTCGCGCCCCCCGCGGGGGCGTGGATCGAATCCGTGTTTAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                    <  1:4528381/98‑1 (MQ=37)
                                                   ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                   >  1:4586799/1‑101 (MQ=255)
                                                    tGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                  <  2:4345764/101‑1 (MQ=255)
                                                     ccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                   <  1:1728763/99‑1 (MQ=2)
                                                      cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                  >  1:3824057/2‑100 (MQ=2)
                                                      aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCgg                                                >  2:4053705/1‑101 (MQ=255)
                                                       gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCggc                                               >  1:2067277/1‑100 (MQ=255)
                                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatgg                                               <  2:3343935/101‑5 (MQ=2)
                                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCCGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGATACCGGTcc                                             <  2:1780785/101‑1 (MQ=255)
                                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACCGGTcc                                             >  1:1961854/1‑101 (MQ=255)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggc                                              >  1:2617467/1‑96 (MQ=2)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                            <  2:1411537/101‑1 (MQ=255)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                            >  1:2878921/1‑101 (MQ=255)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGGCCa                                            >  2:3325993/1‑101 (MQ=255)
                                                           gacccgccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggct                                             >  2:380749/3‑95 (MQ=0)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggct                                             <  1:2776107/101‑7 (MQ=2)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggct                                             >  2:2290199/1‑95 (MQ=2)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCag                                           <  1:2078402/101‑1 (MQ=255)
                                                            tCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggcta                                            >  1:535857/1‑94 (MQ=2)
                                                            tCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                                          >  1:3061794/1‑101 (MQ=255)
                                                            tCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                                          >  2:4533047/1‑101 (MQ=255)
                                                            tCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                                          <  1:4672457/101‑1 (MQ=255)
                                                            tCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                                          <  1:2427635/101‑1 (MQ=255)
                                                            gccccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggcta                                            <  2:1022264/100‑8 (MQ=2)
                                                            cccccccGCGGGGGCGTGTATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                                          <  2:3283189/100‑1 (MQ=255)
                                                             ccccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCagag                                         <  1:3737106/101‑1 (MQ=255)
                                                              cccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctatg                                          >  1:2144460/1‑92 (MQ=2)
                                                              cccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctatg                                          <  2:1892419/101‑10 (MQ=2)
                                                              cccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGGGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctatg                                          >  1:2295562/1‑92 (MQ=2)
                                                                cccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATa                                      <  1:3225671/101‑1 (MQ=255)
                                                                     gggggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                                 >  2:983359/1‑101 (MQ=255)
                                                                      ggggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgc                                >  1:444125/1‑101 (MQ=255)
                                                                      ggggCGTGAATCGAAACCGTGTGGAG...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGGGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgc                                <  2:2535560/101‑1 (MQ=255)
                                                                           gTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGa                           >  1:1446318/1‑101 (MQ=255)
                                                                            tGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                          >  2:3504193/1‑101 (MQ=255)
                                                                            tGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                          >  1:2025885/1‑101 (MQ=255)
                                                                            tGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                          <  1:3605618/101‑1 (MQ=255)
                                                                                  gAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                    >  2:358349/1‑101 (MQ=255)
                                                                                  gAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                    >  2:2374583/1‑101 (MQ=255)
                                                                                  gAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                    <  1:3343698/101‑1 (MQ=255)
                                                                                   aaaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                   >  2:3212944/1‑101 (MQ=255)
                                                                                   aaaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                   >  2:2234421/1‑101 (MQ=255)
                                                                                   aaaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                   >  1:2048597/1‑101 (MQ=255)
                                                                                   aaaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                   <  2:1338470/101‑1 (MQ=255)
                                                                                        gtgtGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGt              <  2:2077941/101‑1 (MQ=255)
                                                                                         tgtgGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTc             <  1:1384536/101‑1 (MQ=255)
                                                                                         tgtgGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTc             >  2:2594380/1‑101 (MQ=255)
                                                                                          gtgGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCg            <  2:1157435/101‑1 (MQ=255)
                                                                                            tctt...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCCCt          <  1:2707301/98‑1 (MQ=255)
                                                                                            ggAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCt          <  2:2044918/101‑1 (MQ=255)
                                                                                               t...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGTCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccc       >  1:4344355/1‑101 (MQ=255)
                                                                                               t...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCtaga       >  2:2707147/1‑98 (MQ=255)
                                                                                                   gatCATGCAGCGCGGACGGCTTGGGTCACTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCCGACGTCGCTcccc      >  2:2945177/1‑101 (MQ=255)
                                                                                                      cATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCcg   >  2:2124241/1‑101 (MQ=255)
                                                                                                       |                                                                                                    
CCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGC  >  NC_014034/1396716‑1396916

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG