Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,825 G→T 14.2% R236L (CGG→CTC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,8250G→T14.2% 91.4 0.0 0.0266R236L (CGG→CTG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (20/18):  ref base (121/107):  total (141/125)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 294 total aligned reads displayed.

CGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1396725‑1396925
                                                                                                        |                                                                                                    
cGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGGGGA.T...GATCATGCAGcgcg                                                                                                      >  2:587927/1‑101 (MQ=255)
cGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGTGTGGA.A...GATCATGCAGcacg                                                                                                      >  2:4712482/1‑101 (MQ=255)
 gTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCgg                                                                                                     >  2:2403500/1‑101 (MQ=255)
 gTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCgg                                                                                                     >  2:2335505/1‑101 (MQ=255)
   ggATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGAc                                                                                                   <  1:1631709/101‑1 (MQ=255)
    gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACg                                                                                                  >  1:4491954/1‑101 (MQ=255)
       cGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCt                                                                                               >  2:4672201/1‑101 (MQ=255)
       cGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.C...GATCATGCAGCGCGGACGGCt                                                                                               <  1:2688738/101‑1 (MQ=255)
        gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCtt                                                                                              >  2:1346766/1‑101 (MQ=255)
             ccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTc                                                                                         >  2:800414/1‑101 (MQ=255)
             ccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTc                                                                                         <  2:138643/101‑1 (MQ=255)
               tGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCATCGCGGACGGCTTGGGTCGc                                                                                       >  1:422634/1‑101 (MQ=255)
                  cccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGGTTGGGTCGCTcc                                                                                    >  1:686665/1‑101 (MQ=255)
                  cccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTcc                                                                                    >  1:4607819/1‑101 (MQ=255)
                   ccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTccc                                                                                   >  2:76614/1‑101 (MQ=255)
                   ccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTccc                                                                                   >  2:3641176/1‑101 (MQ=255)
                    cTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTcccc                                                                                  <  2:4151104/101‑1 (MQ=255)
                    cTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTcccc                                                                                  >  2:857373/1‑101 (MQ=255)
                     tCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTccccc                                                                                 >  2:3542903/1‑101 (MQ=255)
                     tCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTccccc                                                                                 >  1:2922326/1‑101 (MQ=255)
                     tCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTccccc                                                                                 <  2:2836670/101‑1 (MQ=255)
                       atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTccccccc                                                                               >  1:3851317/1‑100 (MQ=255)
                        tatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGGGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCcgc                                                                              >  1:791465/1‑101 (MQ=255)
                        tatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGGCGCTCCCCCcgc                                                                              >  1:3512848/1‑101 (MQ=255)
                            ctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCgggg                                                                          >  1:2246813/1‑101 (MQ=255)
                             tgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCggggg                                                                         >  1:2281353/1‑101 (MQ=255)
                             tgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCggggg                                                                         >  1:1401983/1‑101 (MQ=255)
                             tgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCggggg                                                                         >  2:3905867/1‑101 (MQ=255)
                             tgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCggggg                                                                         >  1:2002563/1‑101 (MQ=255)
                              gcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGc                                                                        >  1:135136/1‑101 (MQ=255)
                              gcCACGATGCCCTTGAGTCGCTCCCCCCGC.GGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCg                                                                       <  1:1453855/101‑1 (MQ=255)
                               ccACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCg                                                                       >  1:2496674/1‑101 (MQ=255)
                               ccACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCg                                                                       >  1:1688063/1‑101 (MQ=255)
                                cACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCgg                                                                      >  1:3438432/1‑100 (MQ=255)
                                 tcGATGCCATTGGGTGGCTCCCCCCGCGGGGGGGTGGATCGAACCCGTGTGGA.T...GATCATGCACCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGtt                                                                     <  2:3180443/100‑2 (MQ=37)
                                  cGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTgg                                                                    <  2:2143936/101‑1 (MQ=255)
                                   gATGCCCTTGAGTCGCTCCCCC..CGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 >  2:2098062/1‑101 (MQ=255)
                                    tcccatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGA.TCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                    <  1:3100135/91‑1 (MQ=2)
                                    tcacatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGA.TCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                    >  1:3462855/11‑101 (MQ=2)
                                     tGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 <  1:1745824/101‑1 (MQ=255)
                                      gCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                <  2:913741/101‑1 (MQ=255)
                                       catcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGA.TCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 <  2:2039674/94‑1 (MQ=2)
                                       catcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGA.TCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 >  1:937005/8‑101 (MQ=2)
                                        ccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                              <  1:587978/101‑1 (MQ=255)
                                        atcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGA.TCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                <  1:3528556/95‑1 (MQ=2)
                                          ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                            >  2:2215505/1‑101 (MQ=255)
                                          ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                            >  2:117941/1‑101 (MQ=255)
                                          ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGGCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                            >  2:1611749/1‑101 (MQ=255)
                                            gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                                          <  2:3282861/101‑1 (MQ=255)
                                            ccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGA.TCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                            >  2:1368031/3‑101 (MQ=2)
                                            ccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGA.TCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                            >  2:4131594/3‑101 (MQ=2)
                                             cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGA.TCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                           <  2:3374324/100‑2 (MQ=2)
                                             aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCgg                                                         <  2:3984874/101‑1 (MQ=255)
                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCggc                                                        >  1:2067277/1‑100 (MQ=255)
                                               tCGCTCCCCCCGCGGGGGGGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTc                                                       <  1:4181123/101‑1 (MQ=255)
                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGA.TCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGGGGATCGACAcatgg                                                        >  2:1121449/1‑97 (MQ=2)
                                                  gacccgccGCGGGGGCGTGGATCGAAACGGCGTAGA.TCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggct                                                      >  2:380749/3‑95 (MQ=0)
                                                  cTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGGGGGGGTCGAAAACGGTCCag                                                    >  2:3223357/1‑101 (MQ=38)
                                                  cTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCag                                                    <  1:2078402/101‑1 (MQ=255)
                                                   tCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGA.TCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggcta                                                     >  1:535857/1‑94 (MQ=2)
                                                   tCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                                                   <  1:4672457/101‑1 (MQ=255)
                                                   tCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                                                   >  2:4533047/1‑101 (MQ=255)
                                                    ccccccGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCagag                                                  >  2:3295726/1‑101 (MQ=255)
                                                     cccccGCGGGGGCGTGGATCGAAACGGCGTAGA.TCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctatg                                                   <  2:128428/101‑10 (MQ=2)
                                                     cccccGCGGGGGCGTGGATCGAAACGGCGTAGA.TCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctatg                                                   >  1:2144460/1‑92 (MQ=2)
                                                     cccccGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAgaga                                                 <  2:1869762/101‑1 (MQ=255)
                                                       cccGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATa                                               <  1:3225671/101‑1 (MQ=255)
                                                       cccGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATa                                               <  1:3637867/101‑1 (MQ=255)
                                                       cccGCGGGGGCGTGGATCGAAACCG.GTCCAGA...GAT.AGACCGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAg                                              <  2:2067156/101‑1 (MQ=11)
                                                           cgGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAcc                                           >  1:2762696/1‑101 (MQ=255)
                                                            gggggCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                                          <  2:3339640/101‑1 (MQ=255)
                                                            gggggCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                                          >  2:983359/1‑101 (MQ=255)
                                                             ggggCGTGAATCGAAACCGTGTGGA.G...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGGGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgc                                         <  2:2535560/101‑1 (MQ=255)
                                                              gggCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcg                                        >  1:3741817/1‑101 (MQ=255)
                                                                  gTGGATCGAAACCGTGTGGA.T...GATCATGCATCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGa                                    >  1:999129/1‑101 (MQ=255)
                                                                  gTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGa                                    <  2:1770303/101‑1 (MQ=255)
                                                                   tGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                                   >  1:1111625/1‑101 (MQ=255)
                                                                   tGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                                   >  1:2025885/1‑101 (MQ=255)
                                                                   tGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                                   <  2:3438225/101‑1 (MQ=255)
                                                                        cGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct                              >  1:2411336/1‑101 (MQ=255)
                                                                         gAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                             <  1:3343698/101‑1 (MQ=255)
                                                                         gAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                             <  1:4533300/101‑1 (MQ=255)
                                                                         gAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                             >  2:2374583/1‑101 (MQ=255)
                                                                          aaaCCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                            >  1:2048597/1‑101 (MQ=255)
                                                                             ccGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGAc                         <  2:2281220/101‑1 (MQ=255)
                                                                             ccGTGTAGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCCGGTCCAGAGATAGTCCGCGCGGGCCTCCTCAgcc                         <  2:422590/101‑3 (MQ=37)
                                                                              cGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACg                        <  1:1242868/101‑1 (MQ=255)
                                                                                tgtgGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTc                      >  2:2594380/1‑101 (MQ=255)
                                                                                tgtgGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTc                      <  2:4337147/101‑1 (MQ=255)
                                                                                   ggA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCt                   >  1:1094238/1‑101 (MQ=255)
                                                                                     a.t...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGGCCTCATCAGACGTCGCTcc                 >  1:2823765/1‑101 (MQ=255)
                                                                                       t...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGTCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccc                >  1:4344355/1‑101 (MQ=255)
                                                                                       t...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCtaga                >  2:2707147/1‑98 (MQ=255)
                                                                                       t...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccc                <  2:444081/101‑1 (MQ=255)
                                                                                       t...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccc                <  2:196932/101‑1 (MQ=255)
                                                                                           gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcccc               >  1:1893190/1‑101 (MQ=255)
                                                                                           gatCATGCAGCGCGGACGGCTTGGGTCACTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCCGACGTCGCTcccc               >  2:2945177/1‑101 (MQ=255)
                                                                                                  cAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCggg        >  1:757580/1‑101 (MQ=255)
                                                                                                    gcgcGGACGGCTTGGGTCGCGCCCCCCGCGGGGGCGTGGATCGAAGCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCggggg      <  1:3992667/101‑1 (MQ=255)
                                                                                                       cgGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGt   <  1:3906109/101‑1 (MQ=255)
                                                                                                        |                                                                                                    
CGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1396725‑1396925

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG