Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,826 G→C 14.2% R236R (CGG→CTC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,8260G→C14.2% 85.7 0.0 0.0266R236R (CGG→CGC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (20/18):  ref base (119/109):  total (139/127)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

GTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1396726‑1396926
                                                                                                        |                                                                                                    
gTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGT.T...GATCATGCAGCGCgg                                                                                                      >  2:3387257/1‑101 (MQ=255)
gTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCgg                                                                                                      <  2:3726818/101‑1 (MQ=255)
gTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCgg                                                                                                      >  2:2335505/1‑101 (MQ=255)
 tGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCggc                                                                                                     >  2:1909952/1‑100 (MQ=255)
 tGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGa                                                                                                     >  1:913818/1‑101 (MQ=255)
  ggATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGAc                                                                                                    <  1:1631709/101‑1 (MQ=255)
   gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACg                                                                                                   >  1:4491954/1‑101 (MQ=255)
      cGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.C...GATCATGCAGCGCGGACGGCt                                                                                                <  1:2688738/101‑1 (MQ=255)
       gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCCGCGCGGACGGCtt                                                                                               >  2:384548/1‑101 (MQ=255)
       gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCtt                                                                                               >  2:1346766/1‑101 (MQ=255)
            ccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTc                                                                                          <  2:138643/101‑1 (MQ=255)
            ccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTc                                                                                          >  2:800414/1‑101 (MQ=255)
              tGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCATCGCGGACGGCTTGGGTCGc                                                                                        >  1:422634/1‑101 (MQ=255)
                 cccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTcc                                                                                     >  1:4607819/1‑101 (MQ=255)
                  ccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTccc                                                                                    >  2:76614/1‑101 (MQ=255)
                   cTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTcccc                                                                                   >  2:857373/1‑101 (MQ=255)
                    tCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTccccc                                                                                  <  2:2836670/101‑1 (MQ=255)
                    tCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTccccc                                                                                  >  1:2922326/1‑101 (MQ=255)
                     cATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTcccccc                                                                                 >  1:2171028/1‑101 (MQ=255)
                      atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCcg                                                                                <  2:4450054/101‑1 (MQ=255)
                       tatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCcgc                                                                               >  1:570508/1‑101 (MQ=255)
                        atGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCcgcg                                                                              >  2:3966775/1‑101 (MQ=255)
                           ctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCgggg                                                                           <  2:4144006/101‑1 (MQ=255)
                            tgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCggggg                                                                          >  1:1401983/1‑101 (MQ=255)
                            tgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCggggg                                                                          >  1:2281353/1‑101 (MQ=255)
                            tgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCggggg                                                                          >  1:2002563/1‑101 (MQ=255)
                             gcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGc                                                                         >  1:135136/1‑101 (MQ=255)
                             gcCACGATGCCCTTGAGTCGCTCCCCCCGC.GGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCg                                                                        <  1:1453855/101‑1 (MQ=255)
                              ccACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCg                                                                        >  1:213734/1‑101 (MQ=255)
                              ccACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCg                                                                        >  1:1688063/1‑101 (MQ=255)
                              ccACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCg                                                                        >  1:2496674/1‑101 (MQ=255)
                               cACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCgg                                                                       >  1:3438432/1‑100 (MQ=255)
                               cACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGGGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCGCCGCggggggcc                                                                       >  1:649874/1‑98 (MQ=255)
                                tcGATGCCATTGGGTGGCTCCCCCCGCGGGGGGGTGGATCGAACCCGTGTGGA.T...GATCATGCACCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGtt                                                                      <  2:3180443/100‑2 (MQ=37)
                                 cGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTgg                                                                     <  2:2143936/101‑1 (MQ=255)
                                  gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                    >  1:773102/1‑101 (MQ=255)
                                  gATGCCCTTGAGTCGCTCCCCC..CGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  >  2:2098062/1‑101 (MQ=255)
                                   tcccatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGA.TCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                     <  1:3100135/91‑1 (MQ=2)
                                   tcacatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGA.TCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                     >  1:3462855/11‑101 (MQ=2)
                                   tcacatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGA.TCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                     <  2:2595314/91‑1 (MQ=2)
                                    tGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  <  1:1745824/101‑1 (MQ=255)
                                     gCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                 <  2:913741/101‑1 (MQ=255)
                                     acatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGA.TCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                   >  2:1104407/9‑101 (MQ=2)
                                         ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                             >  2:117941/1‑101 (MQ=255)
                                         ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                             >  2:2215505/1‑101 (MQ=255)
                                         ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGGCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                             >  2:1611749/1‑101 (MQ=255)
                                         cgccggcGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGA.TCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                               <  1:713129/95‑1 (MQ=2)
                                          tGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                            <  2:4345764/101‑1 (MQ=255)
                                           ccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGA.TCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                             >  2:1368031/3‑101 (MQ=2)
                                           ccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGA.TCTCGGTCTGGGGGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                             >  2:2411101/3‑101 (MQ=2)
                                            cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGA.TCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                            >  1:3824057/2‑100 (MQ=2)
                                            aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCgg                                                          >  2:4053705/1‑101 (MQ=255)
                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCggc                                                         >  1:2067277/1‑100 (MQ=255)
                                              tCGCTCCCCCCGCGGGGGGGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTc                                                        <  1:4181123/101‑1 (MQ=255)
                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGA.TCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatg                                                          >  2:4159995/1‑98 (MQ=2)
                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTc                                                        >  2:186199/1‑101 (MQ=255)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGA.TCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatgg                                                         <  2:3343935/101‑5 (MQ=2)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                                       <  2:2010521/101‑1 (MQ=255)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGGCCa                                                      >  2:3325993/1‑101 (MQ=255)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGGGGGGGTCGAAAACGGTCCag                                                     >  2:3223357/1‑101 (MQ=38)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCag                                                     <  1:2078402/101‑1 (MQ=255)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                                                    <  1:4672457/101‑1 (MQ=255)
                                                  cccccccGCGGGGGCGTGTATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                                                    <  2:3283189/100‑1 (MQ=255)
                                                   ccccccGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCagag                                                   <  1:3737106/101‑1 (MQ=255)
                                                    cccccGCGGGGGCGTGGATCGAAACGGCGTAGA.TCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctatg                                                    <  2:128428/101‑10 (MQ=2)
                                                      cccGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATa                                                <  2:2537261/101‑1 (MQ=255)
                                                      cccGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATa                                                <  1:3225671/101‑1 (MQ=255)
                                                      cccGCGGGGGCGTGGATCGAAACCG.GTCCAGA...GAT.AGACCGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAg                                               <  2:2067156/101‑1 (MQ=11)
                                                          cgGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAcc                                            >  1:2762696/1‑101 (MQ=255)
                                                          cgGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAcc                                            <  2:4626070/101‑1 (MQ=255)
                                                           gggggCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                                           >  2:983359/1‑101 (MQ=255)
                                                            ggggCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgc                                          <  1:983436/101‑1 (MQ=255)
                                                             gggCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcg                                         <  2:4246026/101‑1 (MQ=255)
                                                                 gTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGa                                     <  2:1770303/101‑1 (MQ=255)
                                                                  tGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                                    >  1:1111625/1‑101 (MQ=255)
                                                                  tGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                                    >  1:2025885/1‑101 (MQ=255)
                                                                  tGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                                    <  1:3605618/101‑1 (MQ=255)
                                                                  tGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                                    >  2:3504193/1‑101 (MQ=255)
                                                                       cGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct                               >  1:2411336/1‑101 (MQ=255)
                                                                       cGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct                               >  2:2392850/1‑101 (MQ=255)
                                                                        gAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                              <  1:4533300/101‑1 (MQ=255)
                                                                        gAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                              >  2:358349/1‑101 (MQ=255)
                                                                        gAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                              <  1:3343698/101‑1 (MQ=255)
                                                                        gAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                              <  1:351052/101‑1 (MQ=255)
                                                                         aaaCCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                             >  1:2048597/1‑101 (MQ=255)
                                                                            ccGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGAc                          <  2:2281220/101‑1 (MQ=255)
                                                                             cGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACg                         <  1:1242868/101‑1 (MQ=255)
                                                                                  ggA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCt                    >  1:1094238/1‑101 (MQ=255)
                                                                                  ggA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCt                    <  2:2044918/101‑1 (MQ=255)
                                                                                    a.t...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGGCCTCATCAGACGTCGCTcc                  >  1:2823765/1‑101 (MQ=255)
                                                                                    a.t...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcc                  <  2:4679243/101‑1 (MQ=255)
                                                                                    a.t...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcc                  >  1:470449/1‑101 (MQ=255)
                                                                                      t...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGTCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccc                 >  1:4344355/1‑101 (MQ=255)
                                                                                      t...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCtaga                 >  2:2707147/1‑98 (MQ=255)
                                                                                          gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcccc                >  1:1893190/1‑101 (MQ=255)
                                                                                          gatCATGCAGCGCGGACGGCTTGGGTCACTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCCGACGTCGCTcccc                >  2:2945177/1‑101 (MQ=255)
                                                                                           atCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccccc               <  2:526362/101‑1 (MQ=255)
                                                                                                gCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCgg          <  1:3522986/101‑1 (MQ=255)
                                                                                                   gcgcGGACGGCTTGGGTCGCGCCCCCCGCGGGGGCGTGGATCGAAGCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCggggg       <  1:3992667/101‑1 (MQ=255)
                                                                                                      cgGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGt    <  1:3906109/101‑1 (MQ=255)
                                                                                                        |                                                                                                    
GTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGA.T...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1396726‑1396926

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG