Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,133 T→A 5.2% S339T (TCC→AAA)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,1330T→A 5.2% 25.8 ‑0.6 ‑0.9229S339T (TCC→ACC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/5):  ref base (99/118):  total (106/123)
Fisher's exact test strand distribution p-value = 5.54e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.38e-01

Only 100 of 238 total aligned reads displayed.

CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCC  >  NC_014034/1397033‑1397233
                                                                                                    |                                                                                                      
cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGt                                                                                                        <  2:470405/101‑1 (MQ=255)
  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTcc                                                                                                      <  1:3857175/101‑1 (MQ=255)
    gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAg                                                                                                    <  1:1969664/101‑1 (MQ=255)
    gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAg                                                                                                    <  1:2104007/101‑1 (MQ=255)
     cTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGc                                                                                                   <  2:1379498/101‑1 (MQ=255)
      tCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGCTCGGAACGGCTAGcc                                                                                                  >  1:758899/1‑100 (MQ=38)
             gcgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCca                                                                                           <  1:1034928/101‑1 (MQ=255)
             gcgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCca                                                                                           <  2:1848432/101‑1 (MQ=255)
              cgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcac                                                                                          >  1:271767/1‑101 (MQ=255)
              cgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcac                                                                                          >  1:1458417/1‑101 (MQ=255)
               gggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcaca                                                                                         >  2:2561271/1‑101 (MQ=255)
               gggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcaca                                                                                         <  1:2147770/101‑1 (MQ=255)
                ggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACAt                                                                                        <  2:1995309/101‑1 (MQ=255)
                ggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACAt                                                                                        <  1:1810875/101‑1 (MQ=255)
                   gCGTGGCTCGAAATTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTc                                                                                     <  2:712500/101‑1 (MQ=255)
                   gCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTc                                                                                     <  1:4665919/101‑1 (MQ=255)
                   gCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTc                                                                                     <  1:4402610/101‑1 (MQ=255)
                     gTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCtt                                                                                   <  2:3063021/101‑1 (MQ=255)
                      tGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc                                                                                  <  1:3057873/101‑1 (MQ=255)
                      tGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc                                                                                  <  1:3754649/101‑1 (MQ=255)
                      gtgATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc                                                                                  <  2:113092/99‑1 (MQ=255)
                       ggATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCCGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCg                                                                                 >  2:305605/1‑101 (MQ=255)
                        gATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGa                                                                                >  1:2519938/1‑101 (MQ=255)
                          tCGAAACTGCCGTCTTGAGTGACGCGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAcc                                                                              <  2:1625100/101‑1 (MQ=255)
                           cGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.g                                                                            >  2:2393568/1‑101 (MQ=255)
                           cGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.g                                                                            <  1:3338480/101‑1 (MQ=255)
                             aaaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.gcg                                                                          >  1:2115418/1‑99 (MQ=255)
                             aaaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.Tg                                                                         <  2:152116/101‑1 (MQ=255)
                             aaaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.Tg                                                                         <  2:2716318/101‑1 (MQ=255)
                              aaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.Tgc                                                                        <  2:548704/101‑1 (MQ=255)
                               aCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.Tgcg                                                                       >  1:2447076/1‑101 (MQ=255)
                                       cTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCt                                                               <  1:4178839/101‑1 (MQ=255)
                                         tGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTcc                                                             >  2:3362245/1‑101 (MQ=255)
                                         tGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTcc                                                             >  2:832805/1‑101 (MQ=255)
                                          gAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTccc                                                            >  1:168691/1‑101 (MQ=255)
                                            gTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTccccc                                                          <  2:4246629/101‑1 (MQ=255)
                                            gTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTccccc                                                          <  1:2413697/101‑1 (MQ=255)
                                            gTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTccccc                                                          <  1:3256782/101‑1 (MQ=255)
                                             tGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTcccccc                                                         <  2:2635410/101‑1 (MQ=255)
                                             tGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTcccccc                                                         <  2:2170904/101‑1 (MQ=255)
                                               aTGGGGCGCGAGCAGATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCACGCCACATGTCTTCGGCC.G.TGCGCCGTCGCTCCCCCcgc                                                       <  2:4607566/101‑1 (MQ=255)
                                                tGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCcgcg                                                      >  2:3918034/1‑101 (MQ=255)
                                                 ggggCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCgg                                                     <  1:2831897/101‑1 (MQ=255)
                                                     cgcgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGc                                                 <  2:2446940/101‑1 (MQ=255)
                                                     cgcgAGCACATGTCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGc                                                 <  2:3244668/101‑1 (MQ=255)
                                                      aggcgccggtGTCGTCGCCCCCCCCGCGGCGGCGTGGATGGAAAC.TGCGCCGCCCATCGCATCCGGTCGAGC.GCCGCGCAGTCGCTCCCCCCGCGGGGGCg                                                <  2:783705/92‑1 (MQ=37)
                                                        gAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                              >  1:2340264/1‑101 (MQ=255)
                                                        gAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                              <  2:3979773/101‑1 (MQ=255)
                                                         aGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                             <  2:2799739/101‑1 (MQ=255)
                                                         aGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                             >  1:1360129/1‑101 (MQ=255)
                                                         aGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGCATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                             <  1:4045040/101‑1 (MQ=255)
                                                           cggctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                           >  1:2951665/9‑101 (MQ=0)
                                                           cacaTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                           <  2:3460973/101‑1 (MQ=255)
                                                            ggctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                          <  1:139395/94‑1 (MQ=0)
                                                            acaTGTCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                          <  2:2933048/101‑1 (MQ=255)
                                                             gctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                         <  2:3722109/95‑1 (MQ=0)
                                                             caTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                         <  2:3909268/101‑1 (MQ=255)
                                                             caTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                         <  2:3769620/101‑1 (MQ=255)
                                                               tgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                       <  1:3120889/97‑1 (MQ=2)
                                                               tGTCGTCGCTCCCCCCGGGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGGCC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                       <  1:1999606/101‑1 (MQ=255)
                                                                gtcgtcGCCCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      <  1:3547074/101‑1 (MQ=255)
                                                                gtcgtcCCTCCCTCCGCGGGGGCGCGGAGCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      <  2:627128/101‑1 (MQ=38)
                                                                 tcgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                     <  2:4743020/101‑1 (MQ=255)
                                                                 gggttgtcacccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                     >  2:1877035/10‑101 (MQ=255)
                                                                  ggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                    >  2:127625/2‑100 (MQ=2)
                                                                    tcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACggg                                  >  2:3146684/1‑99 (MQ=255)
                                                                     cgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgtgt                                 >  2:2217932/1‑101 (MQ=255)
                                                                        tCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTg                              >  1:641816/1‑101 (MQ=255)
                                                                         ccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGc                             >  1:61112/1‑101 (MQ=255)
                                                                         ccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGc                             <  2:4189059/101‑1 (MQ=255)
                                                                          cccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGcc                            >  1:4462649/1‑101 (MQ=255)
                                                                           ccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa                           <  1:3918276/101‑1 (MQ=255)
                                                                            cccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCac                          <  1:4357688/101‑1 (MQ=255)
                                                                             ccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacg                         >  2:3222534/1‑101 (MQ=255)
                                                                             ccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacg                         <  1:4738716/101‑1 (MQ=255)
                                                                               gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgac                       <  2:2519793/101‑1 (MQ=255)
                                                                               gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgac                       <  2:3666263/101‑1 (MQ=255)
                                                                                 gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGc                     <  1:118147/101‑1 (MQ=255)
                                                                                  ggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGcc                    >  2:1577306/1‑101 (MQ=255)
                                                                                    ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGcccc                  >  1:957424/1‑100 (MQ=255)
                                                                                    ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCt                  <  1:3385895/101‑1 (MQ=255)
                                                                                    ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCt                  <  2:1719867/101‑1 (MQ=255)
                                                                                    ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCt                  <  1:2861539/101‑1 (MQ=255)
                                                                                     gCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTg                 <  1:4641611/101‑1 (MQ=255)
                                                                                       gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGCCGCCCTGCa               >  1:4499073/1‑101 (MQ=255)
                                                                                       gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCa               >  2:3526301/1‑101 (MQ=255)
                                                                                       gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCa               >  2:2660611/1‑101 (MQ=255)
                                                                                         ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCAcc             >  1:2757611/1‑101 (MQ=255)
                                                                                         ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCAcc             <  1:3450517/101‑1 (MQ=255)
                                                                                          gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCa            >  2:1115508/1‑101 (MQ=255)
                                                                                           aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAg           <  1:1128313/101‑1 (MQ=255)
                                                                                           aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACGTGTCTGCCACGACGCCCTGCACCAg           <  1:2660765/101‑1 (MQ=255)
                                                                                            tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGa          <  2:4563672/101‑1 (MQ=255)
                                                                                            tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGa          >  1:3711049/1‑101 (MQ=255)
                                                                                             cGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGAt         <  2:2274331/101‑1 (MQ=255)
                                                                                              gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATc        >  1:3232409/1‑101 (MQ=255)
                                                                                              gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATc        >  1:489581/1‑101 (MQ=255)
                                                                                               aaaCGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATcc       >  2:4335975/1‑101 (MQ=255)
                                                                                                 aCGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCcgc     >  1:2242446/1‑101 (MQ=255)
                                                                                                    tCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGcc  <  1:1718540/101‑1 (MQ=255)
                                                                                                    |                                                                                                      
CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCC  >  NC_014034/1397033‑1397233

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG