Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,134 C→A 5.3% S339Y (TCC→AAA)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,1340C→A 5.3% 22.7 ‑0.9 ‑0.2227S339Y (TCC→TAC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/5):  ref base (97/118):  total (104/123)
Fisher's exact test strand distribution p-value = 3.91e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.01e-01

Only 100 of 238 total aligned reads displayed.

GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCG  >  NC_014034/1397034‑1397234
                                                                                                    |                                                                                                      
 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTcc                                                                                                       <  1:3857175/101‑1 (MQ=255)
  cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCa                                                                                                      >  2:4741842/1‑101 (MQ=255)
   gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAg                                                                                                     <  1:1969664/101‑1 (MQ=255)
   gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAg                                                                                                     <  1:2104007/101‑1 (MQ=255)
    cTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGc                                                                                                    <  2:1379498/101‑1 (MQ=255)
     tCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGCTCGGAACGGCTAGcc                                                                                                   >  1:758899/1‑100 (MQ=38)
            gcgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCca                                                                                            <  1:1034928/101‑1 (MQ=255)
            gcgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCca                                                                                            <  2:1848432/101‑1 (MQ=255)
             cgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcac                                                                                           >  1:271767/1‑101 (MQ=255)
             cgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcac                                                                                           >  1:1458417/1‑101 (MQ=255)
              gggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcaca                                                                                          >  2:2561271/1‑101 (MQ=255)
              gggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcaca                                                                                          <  1:2147770/101‑1 (MQ=255)
               ggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACAt                                                                                         <  2:1995309/101‑1 (MQ=255)
               ggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACAt                                                                                         <  1:1810875/101‑1 (MQ=255)
                  gCGTGGCTCGAAATTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTc                                                                                      <  2:712500/101‑1 (MQ=255)
                  gCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTc                                                                                      <  1:4665919/101‑1 (MQ=255)
                  gCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTc                                                                                      <  1:4402610/101‑1 (MQ=255)
                    gTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCtt                                                                                    <  2:3063021/101‑1 (MQ=255)
                     tGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc                                                                                   <  1:3057873/101‑1 (MQ=255)
                     tGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc                                                                                   <  1:3754649/101‑1 (MQ=255)
                     gtgATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc                                                                                   <  2:113092/99‑1 (MQ=255)
                      ggATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCCGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCg                                                                                  >  2:305605/1‑101 (MQ=255)
                       gATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGa                                                                                 >  1:2519938/1‑101 (MQ=255)
                         tCGAAACTGCCGTCTTGAGTGACGCGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAcc                                                                               <  2:1625100/101‑1 (MQ=255)
                          cGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.g                                                                             >  2:2393568/1‑101 (MQ=255)
                          cGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.g                                                                             <  1:3338480/101‑1 (MQ=255)
                            aaaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.gcg                                                                           >  1:2115418/1‑99 (MQ=255)
                            aaaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.Tg                                                                          <  2:152116/101‑1 (MQ=255)
                            aaaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.Tg                                                                          <  2:2716318/101‑1 (MQ=255)
                              aCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.Tgcg                                                                        >  1:2447076/1‑101 (MQ=255)
                                      cTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACCTGTCTTCGACC.G.TGCGCCGTCGCt                                                                >  2:516918/1‑101 (MQ=255)
                                      cTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCt                                                                <  1:4178839/101‑1 (MQ=255)
                                        tGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTcc                                                              >  2:3362245/1‑101 (MQ=255)
                                        tGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTcc                                                              >  2:832805/1‑101 (MQ=255)
                                         gAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTccc                                                             >  1:168691/1‑101 (MQ=255)
                                           gTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTccccc                                                           <  2:4246629/101‑1 (MQ=255)
                                           gTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTccccc                                                           <  1:2413697/101‑1 (MQ=255)
                                           gTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTccccc                                                           <  1:3256782/101‑1 (MQ=255)
                                            tGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTcccccc                                                          <  2:2635410/101‑1 (MQ=255)
                                            tGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTcccccc                                                          <  2:2170904/101‑1 (MQ=255)
                                              aTGGGGCGCGAGCAGATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCACGCCACATGTCTTCGGCC.G.TGCGCCGTCGCTCCCCCcgc                                                        <  2:4607566/101‑1 (MQ=255)
                                               tGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCcgcg                                                       >  2:3918034/1‑101 (MQ=255)
                                                ggggCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCgg                                                      <  1:2831897/101‑1 (MQ=255)
                                                    cgcgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGc                                                  <  2:2446940/101‑1 (MQ=255)
                                                    cgcgAGCACATGTCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGc                                                  <  2:3244668/101‑1 (MQ=255)
                                                     aggcgccggtGTCGTCGCCCCCCCCGCGGCGGCGTGGATGGAAAC.TGCGCCGCCCATCGCATCCGGTCGAGC.GCCGCGCAGTCGCTCCCCCCGCGGGGGCg                                                 <  2:783705/92‑1 (MQ=37)
                                                       gAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                               >  1:2340264/1‑101 (MQ=255)
                                                       gAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                               <  2:3979773/101‑1 (MQ=255)
                                                        aGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                              <  2:2799739/101‑1 (MQ=255)
                                                        aGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                              >  1:1360129/1‑101 (MQ=255)
                                                        aGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGCATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                              <  1:4045040/101‑1 (MQ=255)
                                                          cggctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                            >  1:2951665/9‑101 (MQ=0)
                                                          cacaTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                            <  2:3460973/101‑1 (MQ=255)
                                                           ggctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                           <  1:139395/94‑1 (MQ=0)
                                                           acaTGTCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                           <  2:2933048/101‑1 (MQ=255)
                                                            gctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                          <  2:3722109/95‑1 (MQ=0)
                                                            caTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                          <  2:3769620/101‑1 (MQ=255)
                                                            caTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                          <  2:3909268/101‑1 (MQ=255)
                                                              tgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        <  1:3120889/97‑1 (MQ=2)
                                                              tGTCGTCGCTCCCCCCGGGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGGCC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        <  1:1999606/101‑1 (MQ=255)
                                                               gtcgtcGCCCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       <  1:3547074/101‑1 (MQ=255)
                                                               gtcgtcCCTCCCTCCGCGGGGGCGCGGAGCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       <  2:627128/101‑1 (MQ=38)
                                                                gggttgtcacccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                      >  2:1877035/10‑101 (MQ=255)
                                                                 ggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                     >  2:127625/2‑100 (MQ=2)
                                                                   tcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACggg                                   >  2:3146684/1‑99 (MQ=255)
                                                                    cgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgtgt                                  >  2:2217932/1‑101 (MQ=255)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTg                               >  1:641816/1‑101 (MQ=255)
                                                                        ccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGc                              >  1:61112/1‑101 (MQ=255)
                                                                        ccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGc                              <  2:4189059/101‑1 (MQ=255)
                                                                         cccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGcc                             >  1:4462649/1‑101 (MQ=255)
                                                                          ccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa                            <  1:3918276/101‑1 (MQ=255)
                                                                           cccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCac                           <  1:4357688/101‑1 (MQ=255)
                                                                            ccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacg                          <  1:4738716/101‑1 (MQ=255)
                                                                            ccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacg                          >  2:3222534/1‑101 (MQ=255)
                                                                              gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgac                        <  2:2519793/101‑1 (MQ=255)
                                                                              gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgac                        <  2:3666263/101‑1 (MQ=255)
                                                                                gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGc                      <  1:118147/101‑1 (MQ=255)
                                                                                 ggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGcc                     >  2:1577306/1‑101 (MQ=255)
                                                                                   ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGcccc                   >  1:957424/1‑100 (MQ=255)
                                                                                   ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCt                   <  2:1719867/101‑1 (MQ=255)
                                                                                   ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCt                   <  1:2861539/101‑1 (MQ=255)
                                                                                   ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCt                   <  1:3385895/101‑1 (MQ=255)
                                                                                    gCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTg                  <  1:4641611/101‑1 (MQ=255)
                                                                                      gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGCCGCCCTGCa                >  1:4499073/1‑101 (MQ=255)
                                                                                      gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCa                >  2:2660611/1‑101 (MQ=255)
                                                                                      gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCa                >  2:3526301/1‑101 (MQ=255)
                                                                                        ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCAcc              <  1:3450517/101‑1 (MQ=255)
                                                                                        ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCAcc              >  1:2757611/1‑101 (MQ=255)
                                                                                         gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCa             >  2:1115508/1‑101 (MQ=255)
                                                                                          aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAg            <  1:1128313/101‑1 (MQ=255)
                                                                                          aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACGTGTCTGCCACGACGCCCTGCACCAg            <  1:2660765/101‑1 (MQ=255)
                                                                                           tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGa           <  2:4563672/101‑1 (MQ=255)
                                                                                           tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGa           >  1:3711049/1‑101 (MQ=255)
                                                                                            cGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGAt          <  2:2274331/101‑1 (MQ=255)
                                                                                             gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATc         >  1:3232409/1‑101 (MQ=255)
                                                                                             gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATc         >  1:489581/1‑101 (MQ=255)
                                                                                              aaaCGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATcc        >  2:4335975/1‑101 (MQ=255)
                                                                                                aCGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCcgc      >  1:2242446/1‑101 (MQ=255)
                                                                                                   tCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGcc   <  1:1718540/101‑1 (MQ=255)
                                                                                                    ccAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCg  <  2:61105/101‑1 (MQ=255)
                                                                                                    |                                                                                                      
GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCG  >  NC_014034/1397034‑1397234

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG