Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,135 C→A 5.3% S339S (TCC→AAA RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,1350C→A 5.3% 22.7 ‑0.9 ‑0.1227S339S (TCC→TCARCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/5):  ref base (97/118):  total (104/123)
Fisher's exact test strand distribution p-value = 3.91e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.17e-01

Only 100 of 237 total aligned reads displayed.

TCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGT  >  NC_014034/1397035‑1397235
                                                                                                    |                                                                                                      
 cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCa                                                                                                       >  2:4741842/1‑101 (MQ=255)
 cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCa                                                                                                       >  1:3531275/1‑101 (MQ=255)
 cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCa                                                                                                       <  1:1761180/101‑1 (MQ=255)
 cGCTCCCCCCGCGGGGGCATGGATCGAAACTGCCGTCTTGAGTGATGGAGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCa                                                                                                       <  2:3183906/101‑1 (MQ=255)
  gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAg                                                                                                      <  1:2104007/101‑1 (MQ=255)
  gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAg                                                                                                      <  1:1969664/101‑1 (MQ=255)
     ccccccGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTc                                                                                                   <  1:4022710/101‑1 (MQ=255)
           gcgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCca                                                                                             <  1:1034928/101‑1 (MQ=255)
           gcgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCca                                                                                             <  2:1848432/101‑1 (MQ=255)
            cgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcac                                                                                            >  1:271767/1‑101 (MQ=255)
            cgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcac                                                                                            >  1:1458417/1‑101 (MQ=255)
            cgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcac                                                                                            <  2:4620424/101‑1 (MQ=255)
             gggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcaca                                                                                           <  1:2147770/101‑1 (MQ=255)
              ggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACAt                                                                                          <  2:1995309/101‑1 (MQ=255)
               gggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATg                                                                                         <  1:2410305/101‑1 (MQ=255)
                 gCGTGGCTCGAAATTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTc                                                                                       <  2:712500/101‑1 (MQ=255)
                 gCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTc                                                                                       >  1:4373660/1‑101 (MQ=255)
                  cGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCt                                                                                      <  1:4742098/101‑1 (MQ=255)
                   gTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCtt                                                                                     <  2:3063021/101‑1 (MQ=255)
                    tGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc                                                                                    <  1:3754649/101‑1 (MQ=255)
                    tGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc                                                                                    <  1:3057873/101‑1 (MQ=255)
                    gtgATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc                                                                                    <  2:113092/99‑1 (MQ=255)
                     ggATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCg                                                                                   <  2:1553648/101‑1 (MQ=255)
                     ggATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCCGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCg                                                                                   >  2:305605/1‑101 (MQ=255)
                      gATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGa                                                                                  >  1:2519938/1‑101 (MQ=255)
                      gATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGa                                                                                  <  1:3334186/101‑1 (MQ=255)
                        tCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAcc                                                                                >  2:1311867/1‑101 (MQ=255)
                        tCGAAACTGCCGTCTTGAGTGACGCGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAcc                                                                                <  2:1625100/101‑1 (MQ=255)
                         cGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.g                                                                              >  2:10917/1‑101 (MQ=255)
                           aaaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.gcg                                                                            >  1:2115418/1‑99 (MQ=255)
                           aaaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.Tg                                                                           <  2:152116/101‑1 (MQ=255)
                             aCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.Tgcg                                                                         <  1:613139/101‑1 (MQ=255)
                             aCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.Tgcg                                                                         >  1:2447076/1‑101 (MQ=255)
                               tGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGcc                                                                       >  1:793730/1‑101 (MQ=255)
                                gCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCg                                                                      <  2:2737537/101‑1 (MQ=255)
                                  cGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTc                                                                    >  2:1250648/1‑101 (MQ=255)
                                     cTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACCTGTCTTCGACC.G.TGCGCCGTCGCt                                                                 >  2:516918/1‑101 (MQ=255)
                                     cTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCt                                                                 <  2:2235261/101‑1 (MQ=255)
                                     cTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCt                                                                 <  1:4178839/101‑1 (MQ=255)
                                       tGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTcc                                                               >  2:3362245/1‑101 (MQ=255)
                                       tGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTcc                                                               >  2:832805/1‑101 (MQ=255)
                                        gAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTccc                                                              >  1:168691/1‑101 (MQ=255)
                                          gTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTccccc                                                            <  2:4246629/101‑1 (MQ=255)
                                          gTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTccccc                                                            <  1:3256782/101‑1 (MQ=255)
                                           tGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTcccccc                                                           <  2:2170904/101‑1 (MQ=255)
                                           tGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTcccccc                                                           >  1:688163/1‑101 (MQ=255)
                                           tGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTcccccc                                                           <  2:2635410/101‑1 (MQ=255)
                                              tGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCcgcg                                                        >  2:3918034/1‑101 (MQ=255)
                                               ggggCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCgg                                                       <  1:2831897/101‑1 (MQ=255)
                                                  gcgcGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCggggg                                                    <  2:3000981/101‑1 (MQ=255)
                                                   cgcgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGc                                                   <  2:2446940/101‑1 (MQ=255)
                                                   cgcgAGCACATGTCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGc                                                   <  2:3244668/101‑1 (MQ=255)
                                                    aggcgccggtGTCGTCGCCCCCCCCGCGGCGGCGTGGATGGAAAC.TGCGCCGCCCATCGCATCCGGTCGAGC.GCCGCGCAGTCGCTCCCCCCGCGGGGGCg                                                  <  2:783705/92‑1 (MQ=37)
                                                      gAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                <  2:3979773/101‑1 (MQ=255)
                                                      gAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                >  1:2340264/1‑101 (MQ=255)
                                                       aGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                               <  2:2799739/101‑1 (MQ=255)
                                                       aGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                               >  1:1360129/1‑101 (MQ=255)
                                                         cacaTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                             <  2:3460973/101‑1 (MQ=255)
                                                          ggctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  1:139395/94‑1 (MQ=0)
                                                          acaTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  2:1966537/101‑1 (MQ=255)
                                                           gctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                           >  1:4677033/7‑101 (MQ=0)
                                                           gctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  2:3722109/95‑1 (MQ=0)
                                                           caTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  2:3909268/101‑1 (MQ=255)
                                                            aTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                          >  2:3773155/1‑101 (MQ=255)
                                                             tgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         >  2:2432685/5‑101 (MQ=2)
                                                             tgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         <  1:3120889/97‑1 (MQ=2)
                                                             tGTCGTCGCTCCCCCCGGGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGGCC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         <  1:1999606/101‑1 (MQ=255)
                                                              gtcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        <  1:4642606/101‑1 (MQ=255)
                                                              gtcgtcCCTCCCTCCGCGGGGGCGCGGAGCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        <  2:627128/101‑1 (MQ=38)
                                                               tcgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                       >  2:2576990/1‑101 (MQ=255)
                                                                cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                      <  1:2875612/101‑1 (MQ=255)
                                                                       ccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGc                               <  2:4189059/101‑1 (MQ=255)
                                                                        cccccGGGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGcc                              <  1:3223558/101‑1 (MQ=255)
                                                                        cccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGcc                              >  1:4462649/1‑101 (MQ=255)
                                                                         ccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa                             <  1:3918276/101‑1 (MQ=255)
                                                                          cccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCac                            <  1:4357688/101‑1 (MQ=255)
                                                                           ccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacg                           >  2:3222534/1‑101 (MQ=255)
                                                                             gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgac                         <  2:3666263/101‑1 (MQ=255)
                                                                             gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgac                         <  2:2519793/101‑1 (MQ=255)
                                                                             gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgac                         <  1:381051/101‑1 (MQ=255)
                                                                              cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgacg                        <  1:556297/101‑1 (MQ=255)
                                                                               gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGc                       <  1:118147/101‑1 (MQ=255)
                                                                                  ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCt                    <  2:1719867/101‑1 (MQ=255)
                                                                                  ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCt                    <  1:2861539/101‑1 (MQ=255)
                                                                                  ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCt                    <  1:3385895/101‑1 (MQ=255)
                                                                                     gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGCCGCCCTGCa                 >  1:4499073/1‑101 (MQ=255)
                                                                                     gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCa                 >  2:2660611/1‑101 (MQ=255)
                                                                                     gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCa                 >  2:3526301/1‑101 (MQ=255)
                                                                                       ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCAcc               >  1:2757611/1‑101 (MQ=255)
                                                                                       ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCAcc               <  1:3450517/101‑1 (MQ=255)
                                                                                        gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCa              <  1:4628425/101‑1 (MQ=255)
                                                                                         aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAg             <  1:1128313/101‑1 (MQ=255)
                                                                                         aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACGTGTCTGCCACGACGCCCTGCACCAg             <  1:2660765/101‑1 (MQ=255)
                                                                                          tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGa            >  1:3711049/1‑101 (MQ=255)
                                                                                          tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGa            <  2:4563672/101‑1 (MQ=255)
                                                                                           cGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGAt           <  2:2274331/101‑1 (MQ=255)
                                                                                             aaaCGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATcc         >  2:4335975/1‑101 (MQ=255)
                                                                                               aCGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCcgc       >  1:2242446/1‑101 (MQ=255)
                                                                                                  tCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGcc    <  1:1718540/101‑1 (MQ=255)
                                                                                                   ccAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCg   <  2:61105/101‑1 (MQ=255)
                                                                                                    |                                                                                                      
TCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGT  >  NC_014034/1397035‑1397235

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG