Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,141 G→C 5.6% S341S (TCG→CGC RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,1410G→C 5.6% 19.2 ‑1.0 ‑0.0212S341S (TCG→TCCRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/5):  ref base (88/111):  total (95/117)
Fisher's exact test strand distribution p-value = 3.82e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.80e-01

Only 100 of 224 total aligned reads displayed.

CCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTC  >  NC_014034/1397042‑1397240
                                                                                                   |                                                                                                     
ccccGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTcgc                                                                                                      >  1:1719972/1‑101 (MQ=255)
  ccGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCgcgc                                                                                                    >  2:391239/1‑101 (MQ=255)
   cgcgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGcc                                                                                                   >  1:2737701/1‑101 (MQ=255)
    gcgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCca                                                                                                  <  1:1034928/101‑1 (MQ=255)
     cgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcac                                                                                                 <  2:4620424/101‑1 (MQ=255)
     cgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcac                                                                                                 >  1:1458417/1‑101 (MQ=255)
     cgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcac                                                                                                 >  2:3253196/1‑101 (MQ=255)
      gggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcaca                                                                                                <  2:4322873/101‑1 (MQ=255)
      gggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcaca                                                                                                >  2:2561271/1‑101 (MQ=255)
         ggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGt                                                                                             <  2:712615/101‑1 (MQ=255)
          gCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTc                                                                                            <  1:4402610/101‑1 (MQ=255)
          gCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTc                                                                                            <  1:4665919/101‑1 (MQ=255)
           cGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCt                                                                                           <  1:4629190/101‑1 (MQ=255)
           cGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCt                                                                                           >  1:1618178/1‑101 (MQ=255)
           cGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCt                                                                                           <  1:4742098/101‑1 (MQ=255)
            gTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCtt                                                                                          <  2:3063021/101‑1 (MQ=255)
            gTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCtt                                                                                          >  1:2642419/1‑101 (MQ=255)
            gTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCtt                                                                                          >  2:2037595/1‑101 (MQ=255)
             tGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc                                                                                         >  1:633262/1‑101 (MQ=255)
              ggATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCg                                                                                        <  1:2061428/101‑1 (MQ=255)
              ggATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCg                                                                                        <  1:2804534/101‑1 (MQ=255)
              ggATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCCGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCg                                                                                        >  2:305605/1‑101 (MQ=255)
              ggATCGAAACTGCCGGCTTGAGTGATGCGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGAGGATGGAAACGTCCAGCTCGCGCCACATGTCTTCg                                                                                        <  2:1528342/101‑1 (MQ=38)
               gATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGa                                                                                       >  1:2519938/1‑101 (MQ=255)
               gATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGa                                                                                       <  1:3334186/101‑1 (MQ=255)
                  cGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.g                                                                                   <  2:1401889/101‑1 (MQ=255)
                  cGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.g                                                                                   >  2:10917/1‑101 (MQ=255)
                   gAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.t                                                                                 >  2:1707782/1‑101 (MQ=255)
                    aagcTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.Tg                                                                                >  1:2715997/4‑101 (MQ=255)
                    aaaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.Tg                                                                                <  2:2716318/101‑1 (MQ=255)
                     aaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.Tgc                                                                               <  1:2222208/101‑1 (MQ=255)
                      aCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.Tgcg                                                                              <  1:4681821/101‑1 (MQ=255)
                      aCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.Tgcg                                                                              <  2:4198192/101‑1 (MQ=255)
                        tGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGcc                                                                            >  2:217193/1‑101 (MQ=255)
                            gTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCg                                                                        >  2:118131/1‑101 (MQ=255)
                              cTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCt                                                                      <  2:2235261/101‑1 (MQ=255)
                              cTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCt                                                                      >  1:3050380/1‑101 (MQ=255)
                                tGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTcc                                                                    >  2:832805/1‑101 (MQ=255)
                                tGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTcc                                                                    >  2:3362245/1‑101 (MQ=255)
                                tGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTcc                                                                    >  1:4475220/1‑101 (MQ=255)
                                tGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCGcc                                                                    >  1:2301312/1‑101 (MQ=255)
                                  aGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTcccc                                                                  >  2:534638/1‑101 (MQ=255)
                                   gTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTccccc                                                                 <  1:2413697/101‑1 (MQ=255)
                                   gTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTccccc                                                                 <  1:3256782/101‑1 (MQ=255)
                                    tGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTcccccc                                                                <  2:2635410/101‑1 (MQ=255)
                                    tGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTcccccc                                                                >  1:688163/1‑101 (MQ=255)
                                     gATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCcg                                                               >  2:3239124/1‑101 (MQ=255)
                                           gcgcGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCggggg                                                         <  2:3000981/101‑1 (MQ=255)
                                            cgcgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGc                                                        <  1:2110770/101‑1 (MQ=255)
                                            cgcgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGc                                                        <  2:2446940/101‑1 (MQ=255)
                                             aggcgccggtGTCGTCGCCCCCCCCGCGGCGGCGTGGATGGAAAC.TGCGCCGCCCATCGCATCCGGTCGAGC.GCCGCGCAGTCGCTCCCCCCGCGGGGGCg                                                       <  2:783705/92‑1 (MQ=37)
                                               gAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                     <  2:3979773/101‑1 (MQ=255)
                                               gAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                     >  1:1986836/1‑101 (MQ=255)
                                                aGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGCATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                                    <  1:4045040/101‑1 (MQ=255)
                                                 gCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGa                                                   >  2:3562373/1‑101 (MQ=255)
                                                  cacaTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                  <  2:3460973/101‑1 (MQ=255)
                                                   acaTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                 <  2:1966537/101‑1 (MQ=255)
                                                    gctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                >  2:635129/7‑101 (MQ=0)
                                                    gctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                <  2:3722109/95‑1 (MQ=0)
                                                    caTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                <  2:3909268/101‑1 (MQ=255)
                                                    caTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                <  2:3769620/101‑1 (MQ=255)
                                                     ctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                               <  2:2782503/96‑1 (MQ=2)
                                                      tgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                              >  2:2432685/5‑101 (MQ=2)
                                                       gtcgtcGCCCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                             <  1:3547074/101‑1 (MQ=255)
                                                        tcgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                            <  2:4743020/101‑1 (MQ=255)
                                                         ggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                           >  2:127625/2‑100 (MQ=2)
                                                         cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                           <  1:2875612/101‑1 (MQ=255)
                                                          gtcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgt                                          <  1:1694685/101‑1 (MQ=255)
                                                           tcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACggg                                         >  2:3146684/1‑99 (MQ=255)
                                                            cgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgtgt                                        >  1:3347534/1‑101 (MQ=255)
                                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTc                                       >  1:1376891/1‑101 (MQ=255)
                                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTc                                       <  1:4195860/101‑1 (MQ=255)
                                                                  ccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa                                  <  1:3918276/101‑1 (MQ=255)
                                                                   cccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCac                                 <  1:4357688/101‑1 (MQ=255)
                                                                    ccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacg                                <  1:3773390/101‑1 (MQ=255)
                                                                      gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgac                              <  2:3666263/101‑1 (MQ=255)
                                                                      gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgac                              <  2:2519793/101‑1 (MQ=255)
                                                                       cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgacg                             <  1:556297/101‑1 (MQ=255)
                                                                       cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGTGTCTGCCaggacg                             >  2:1862141/1‑101 (MQ=255)
                                                                        gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGc                            <  1:118147/101‑1 (MQ=255)
                                                                         ggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGcc                           >  2:1577306/1‑101 (MQ=255)
                                                                           ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCt                         <  1:1311959/101‑1 (MQ=255)
                                                                            gCGTGGATCGAAACGTCCAGCTCGCGTCACATGTCTTCGTCC.G.TGCTCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTg                        <  2:3918664/101‑1 (MQ=37)
                                                                              gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCa                      >  2:2660611/1‑101 (MQ=255)
                                                                                ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCAcc                    <  1:3450517/101‑1 (MQ=255)
                                                                                 gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCa                   <  1:2393703/101‑1 (MQ=255)
                                                                                  aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAg                  <  1:1128313/101‑1 (MQ=255)
                                                                                  aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAg                  <  1:212568/101‑1 (MQ=255)
                                                                                   tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGa                 >  1:3711049/1‑101 (MQ=255)
                                                                                   tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGa                 <  2:4563672/101‑1 (MQ=255)
                                                                                    cGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGAt                <  2:2274331/101‑1 (MQ=255)
                                                                                    cGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGAt                >  1:2836173/1‑101 (MQ=255)
                                                                                     gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATc               >  2:1782988/1‑101 (MQ=255)
                                                                                     gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATc               >  1:3232409/1‑101 (MQ=255)
                                                                                      aaaCGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATcc              >  2:4335975/1‑101 (MQ=255)
                                                                                      aaaCGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATcc              <  1:3146884/101‑1 (MQ=255)
                                                                                            ccAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCg        <  2:61105/101‑1 (MQ=255)
                                                                                             cAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGt       <  1:4625212/101‑1 (MQ=255)
                                                                                              aGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTc      >  2:3489031/1‑101 (MQ=255)
                                                                                                  cgcgCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTc  <  1:832878/101‑1 (MQ=255)
                                                                                                   |                                                                                                     
CCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTC  >  NC_014034/1397042‑1397240

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG