Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,144 C→A 5.6% R342R (CGC→CGA RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,1440C→A 5.6% 21.7 ‑1.0 ‑0.1214R342R (CGC→CGARCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/5):  ref base (88/113):  total (95/119)
Fisher's exact test strand distribution p-value = 3.79e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.78e-01

Only 100 of 226 total aligned reads displayed.

CCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCC  >  NC_014034/1397044‑1397244
                                                                                                    |                                                                                                      
ccGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCgcgc                                                                                                        >  2:391239/1‑101 (MQ=255)
  gcgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCca                                                                                                      <  1:1034928/101‑1 (MQ=255)
   cgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcac                                                                                                     >  1:1458417/1‑101 (MQ=255)
    gggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcaca                                                                                                    <  2:4322873/101‑1 (MQ=255)
    gggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcaca                                                                                                    <  1:2147770/101‑1 (MQ=255)
    gggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcaca                                                                                                    >  2:2561271/1‑101 (MQ=255)
       ggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGt                                                                                                 <  2:712615/101‑1 (MQ=255)
        gCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTc                                                                                                >  1:4373660/1‑101 (MQ=255)
         cGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCt                                                                                               <  1:4629190/101‑1 (MQ=255)
         cGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCt                                                                                               >  1:1618178/1‑101 (MQ=255)
         cGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCt                                                                                               <  1:4742098/101‑1 (MQ=255)
          gTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCtt                                                                                              <  2:2617178/101‑1 (MQ=255)
          gTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCtt                                                                                              >  2:2037595/1‑101 (MQ=255)
          gTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCtt                                                                                              >  1:2642419/1‑101 (MQ=255)
           tGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc                                                                                             <  1:3057873/101‑1 (MQ=255)
            ggATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCg                                                                                            <  2:3202176/101‑1 (MQ=255)
            ggATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCg                                                                                            <  1:2061428/101‑1 (MQ=255)
            ggATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCg                                                                                            <  1:2804534/101‑1 (MQ=255)
            ggATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCCGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCg                                                                                            >  2:305605/1‑101 (MQ=255)
            ggATCGAAACTGCCGGCTTGAGTGATGCGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGAGGATGGAAACGTCCAGCTCGCGCCACATGTCTTCg                                                                                            <  2:1528342/101‑1 (MQ=38)
             gATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGa                                                                                           >  1:2519938/1‑101 (MQ=255)
             gATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGa                                                                                           <  1:3334186/101‑1 (MQ=255)
                cGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.g                                                                                       <  2:1401889/101‑1 (MQ=255)
                cGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.g                                                                                       >  2:10917/1‑101 (MQ=255)
                 gAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.t                                                                                     >  2:1707782/1‑101 (MQ=255)
                  aagcTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.Tg                                                                                    >  1:2715997/4‑101 (MQ=255)
                   aaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.Tgc                                                                                   <  1:2222208/101‑1 (MQ=255)
                    aCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.Tgcg                                                                                  >  1:2447076/1‑101 (MQ=255)
                    aCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.Tgcg                                                                                  <  1:613139/101‑1 (MQ=255)
                    aCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.Tgcg                                                                                  <  1:4681821/101‑1 (MQ=255)
                      tGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGcc                                                                                >  2:217193/1‑101 (MQ=255)
                       gCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCg                                                                               <  2:3066234/101‑1 (MQ=255)
                          gTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCg                                                                            >  2:118131/1‑101 (MQ=255)
                            cTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCt                                                                          <  2:2235261/101‑1 (MQ=255)
                              tGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTcc                                                                        >  2:832805/1‑101 (MQ=255)
                              tGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTcc                                                                        >  1:4475220/1‑101 (MQ=255)
                              tGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTcc                                                                        >  1:3386178/1‑101 (MQ=255)
                              tGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCGcc                                                                        >  1:2301312/1‑101 (MQ=255)
                                aGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTcccc                                                                      >  2:534638/1‑101 (MQ=255)
                                 gTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTccccc                                                                     <  1:3256782/101‑1 (MQ=255)
                                  tGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTcccccc                                                                    >  1:688163/1‑101 (MQ=255)
                                   gATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCcg                                                                   >  2:3790078/1‑101 (MQ=255)
                                   gATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCcg                                                                   >  2:3239124/1‑101 (MQ=255)
                                    aTGGGGCGCGAGCAGATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCACGCCACATGTCTTCGGCC.G.TGCGCCGTCGCTCCCCCcgc                                                                  <  2:4607566/101‑1 (MQ=255)
                                          cgcgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGc                                                            <  1:2110770/101‑1 (MQ=255)
                                          cgcgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGc                                                            <  2:2446940/101‑1 (MQ=255)
                                           aggcgccggtGTCGTCGCCCCCCCCGCGGCGGCGTGGATGGAAAC.TGCGCCGCCCATCGCATCCGGTCGAGC.GCCGCGCAGTCGCTCCCCCCGCGGGGGCg                                                           <  2:783705/92‑1 (MQ=37)
                                             gAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                         >  1:1986836/1‑101 (MQ=255)
                                             gAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                         <  2:3979773/101‑1 (MQ=255)
                                              aGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGCATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                                        <  1:4045040/101‑1 (MQ=255)
                                                cacaTGTCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                      <  2:3291917/101‑1 (MQ=255)
                                                 ggctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                     <  1:139395/94‑1 (MQ=0)
                                                 acaTGTCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                     <  2:2933048/101‑1 (MQ=255)
                                                  gctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    <  2:3722109/95‑1 (MQ=0)
                                                  caTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    >  2:2333671/1‑101 (MQ=255)
                                                  caTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    <  2:3769620/101‑1 (MQ=255)
                                                   ctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                   <  2:2782503/96‑1 (MQ=2)
                                                    tgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                  >  2:2432685/5‑101 (MQ=2)
                                                     gtcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 <  1:4642606/101‑1 (MQ=255)
                                                     gtcgtcGCCCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 <  1:3547074/101‑1 (MQ=255)
                                                     gtcgtcCCTCCCTCCGCGGGGGCGCGGAGCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 <  2:627128/101‑1 (MQ=38)
                                                      tcgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                <  2:4743020/101‑1 (MQ=255)
                                                       ggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGG.CCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                               >  2:127625/2‑100 (MQ=2)
                                                       cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                               <  1:2875612/101‑1 (MQ=255)
                                                        gtcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgt                                              <  1:1694685/101‑1 (MQ=255)
                                                          cgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgtgt                                            >  1:3347534/1‑101 (MQ=255)
                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTc                                           <  1:4195860/101‑1 (MQ=255)
                                                              ccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGc                                        <  2:4189059/101‑1 (MQ=255)
                                                                 cccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCac                                     <  1:4357688/101‑1 (MQ=255)
                                                                  ccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacg                                    <  1:3773390/101‑1 (MQ=255)
                                                                    gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgac                                  <  2:2519793/101‑1 (MQ=255)
                                                                    gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgac                                  <  2:3666263/101‑1 (MQ=255)
                                                                     cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgacg                                 <  1:556297/101‑1 (MQ=255)
                                                                     cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGTGTCTGCCaggacg                                 >  2:1862141/1‑101 (MQ=255)
                                                                      gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGc                                <  1:118147/101‑1 (MQ=255)
                                                                      gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGc                                >  2:3415694/1‑101 (MQ=255)
                                                                         ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCt                             >  2:2714928/1‑101 (MQ=255)
                                                                         ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCt                             <  1:1311959/101‑1 (MQ=255)
                                                                          gCGTGGATCGAAACGTCCAGCTCGCGTCACATGTCTTCGTCC.G.TGCTCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTg                            <  2:3918664/101‑1 (MQ=37)
                                                                              ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCAcc                        >  1:2757611/1‑101 (MQ=255)
                                                                               gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCa                       <  1:2393703/101‑1 (MQ=255)
                                                                                aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAg                      <  1:1128313/101‑1 (MQ=255)
                                                                                 tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGa                     >  1:3711049/1‑101 (MQ=255)
                                                                                 tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGa                     <  2:4563672/101‑1 (MQ=255)
                                                                                  cGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGAt                    >  1:2836173/1‑101 (MQ=255)
                                                                                   gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATc                   <  1:388861/101‑1 (MQ=255)
                                                                                   gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATc                   >  2:1782988/1‑101 (MQ=255)
                                                                                   gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATc                   >  1:3232409/1‑101 (MQ=255)
                                                                                    aaaCGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATcc                  <  1:3146884/101‑1 (MQ=255)
                                                                                    aaaCGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATcc                  >  2:4335975/1‑101 (MQ=255)
                                                                                    aaaCGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATcc                  >  2:3037254/1‑101 (MQ=255)
                                                                                    aaaCGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAGTCTGCCACGACGCCCTGCACCAGATcc                  >  2:2650/1‑101 (MQ=255)
                                                                                     aaCGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCcg                 <  2:1973916/101‑1 (MQ=255)
                                                                                         tCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGcc             <  2:1615273/101‑1 (MQ=255)
                                                                                          ccAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCg            <  2:61105/101‑1 (MQ=255)
                                                                                           cAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGt           <  1:4625212/101‑1 (MQ=255)
                                                                                            aGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTc          <  2:3531063/101‑1 (MQ=255)
                                                                                            aGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTc          >  2:3489031/1‑101 (MQ=255)
                                                                                                cgcgCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTc      <  1:832878/101‑1 (MQ=255)
                                                                                                  cgcCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTccc    >  1:1900238/1‑101 (MQ=255)
                                                                                                    |                                                                                                      
CCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.G.TGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCC  >  NC_014034/1397044‑1397244

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG