Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,202 T→G 4.0% S362A (TCT→GCT)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2020T→G 4.0% 12.7 ‑3.7 ‑0.2202S362A (TCT→GCT) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/7):  ref base (108/85):  total (110/92)
Fisher's exact test strand distribution p-value = 2.49e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.58e-01

Only 100 of 227 total aligned reads displayed.

CGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGT  >  NC_014034/1397102‑1397302
                                                                                                    |                                                                                                       
 gCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTc                                                                                                        >  1:3696307/1‑101 (MQ=255)
 gCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTc                                                                                                        >  1:1376891/1‑101 (MQ=255)
   tCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTg                                                                                                      >  1:641816/1‑101 (MQ=255)
    ccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGc                                                                                                     >  1:61112/1‑101 (MQ=255)
      ccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa                                                                                                   <  1:3969135/101‑1 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cg                                                                                                <  1:3773390/101‑1 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cg                                                                                                >  2:3222534/1‑101 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cg                                                                                                <  1:4738716/101‑1 (MQ=255)
          gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cgac                                                                                              <  2:2519793/101‑1 (MQ=255)
            gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.Gc                                                                                           <  1:118147/101‑1 (MQ=255)
               ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCt                                                                                        <  1:2861539/101‑1 (MQ=255)
               ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCt                                                                                        <  1:1311959/101‑1 (MQ=255)
                gCGTGGATCGAAACGTCCAGCTCGCGTCACATGTCTTCGTCCGTGCTCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTg                                                                                       <  2:3918664/101‑1 (MQ=37)
                gCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTg                                                                                       <  1:4641611/101‑1 (MQ=255)
                  gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCa                                                                                     >  2:2660611/1‑101 (MQ=255)
                  gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCa                                                                                     >  2:3526301/1‑101 (MQ=255)
                    ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCAcc                                                                                   >  2:885460/1‑101 (MQ=255)
                    ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGTTTGCCA.CTTA.GCCCTGCAcc                                                                                   >  2:73017/1‑101 (MQ=37)
                     gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCa                                                                                  <  1:2393703/101‑1 (MQ=255)
                       tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGa                                                                                >  2:2545050/1‑101 (MQ=255)
                       tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGa                                                                                >  1:3711049/1‑101 (MQ=255)
                        cGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGAt                                                                               <  2:2274331/101‑1 (MQ=255)
                         gAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATc                                                                              <  1:388861/101‑1 (MQ=255)
                         gAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATc                                                                              >  1:3232409/1‑101 (MQ=255)
                         gAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATc                                                                              >  2:1782988/1‑101 (MQ=255)
                          aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATcc                                                                             >  1:1452011/1‑101 (MQ=255)
                          aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATcc                                                                             <  1:2165766/101‑1 (MQ=255)
                            aCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCcgc                                                                           >  1:2242446/1‑101 (MQ=255)
                               tCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGcc                                                                        <  2:1615273/101‑1 (MQ=255)
                                 cAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.Gt                                                                     <  1:4625212/101‑1 (MQ=255)
                                      cgcgCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTc                                                                <  1:832878/101‑1 (MQ=255)
                                         gcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTcccc                                                             <  2:3048076/101‑1 (MQ=255)
                                              aTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcgcg                                                        >  2:1265797/1‑101 (MQ=255)
                                               tGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCgg                                                       <  2:2621619/101‑1 (MQ=255)
                                                     cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCgg                                                 >  1:4487708/1‑100 (MQ=255)
                                                      gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAA.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTg                                                >  2:1876260/1‑101 (MQ=255)
                                                       gccGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTgg                                               <  1:3037442/100‑1 (MQ=255)
                                                        ccGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGa                                              >  2:602040/1‑101 (MQ=255)
                                                         cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                             <  1:3526513/101‑1 (MQ=255)
                                                         cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                             >  1:429603/1‑101 (MQ=255)
                                                          gTGCGCCGGCGCCCTCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGTGGCGTGGATc                                            <  1:4099684/101‑1 (MQ=38)
                                                           tGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGTGGGCGTGGATCg                                           <  1:2577142/101‑1 (MQ=255)
                                                           tGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           >  1:186109/1‑101 (MQ=255)
                                                           ggcgcCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  1:3362447/100‑1 (MQ=255)
                                                            gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCC.AACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  1:1591119/101‑1 (MQ=2)
                                                            gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCC.AACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  1:4562464/101‑1 (MQ=2)
                                                            cgatcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGC.TGGC.GGCCCGGTCGTGAATCTCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                           >  1:4062802/5‑101 (MQ=2)
                                                             tatcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGC.TTGACGCGCTGGATCCGCGGC.GCCGCTCCCCCCGCGGGGGCGTGGATCGa                                          >  1:3204046/4‑101 (MQ=2)
                                                             cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCC.ACCAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                          >  1:1880939/4‑101 (MQ=2)
                                                             cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         <  2:757506/101‑1 (MQ=255)
                                                               gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCC.AACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        <  1:4392604/100‑1 (MQ=2)
                                                               gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCC.AACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        <  2:2727066/100‑1 (MQ=2)
                                                               ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                       <  1:1115594/101‑1 (MQ=255)
                                                               ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                       >  1:3444015/1‑101 (MQ=255)
                                                                cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                      <  2:4474970/101‑1 (MQ=255)
                                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                     >  2:4216297/1‑101 (MQ=255)
                                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                     >  1:1636018/1‑101 (MQ=255)
                                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                     <  2:3728638/101‑1 (MQ=255)
                                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                     >  1:314792/1‑101 (MQ=255)
                                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                     >  1:3324558/1‑101 (MQ=255)
                                                                 gTCGCTCCCCCCGCGGGGGCGTGGACCGAAACCTATGAGGGG.TGGCGGCCCGGTCGTGGATCC.CGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                      <  1:1963394/101‑1 (MQ=0)
                                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAcc                                    >  2:4663004/1‑101 (MQ=255)
                                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                  >  2:636134/1‑101 (MQ=255)
                                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                  >  2:4382313/1‑101 (MQ=255)
                                                                     cTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgcg                                 >  2:3715565/1‑101 (MQ=255)
                                                                       ccccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCa                               >  2:3533596/1‑101 (MQ=255)
                                                                       ccccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCa                               >  2:115182/1‑101 (MQ=255)
                                                                       ccccccGCGGGGGCGTGGATCCAAACGTTTCTGCCA.CGAC.GCCCTGCACCAGCTCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCa                               <  1:3577386/101‑1 (MQ=255)
                                                                        cccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAc                              <  2:1451917/101‑1 (MQ=255)
                                                                            cgcgGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgcgc                          >  2:487673/1‑101 (MQ=255)
                                                                              cgGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGa                        >  2:3392251/1‑101 (MQ=255)
                                                                              cgGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGa                        <  2:2715843/101‑1 (MQ=255)
                                                                               gggggCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGaa                       <  2:4573648/101‑1 (MQ=255)
                                                                               gggggCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGaa                       <  1:2333804/101‑1 (MQ=255)
                                                                               gggggCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGaa                       >  2:1733771/1‑101 (MQ=255)
                                                                                ggggCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAAc                      >  2:3137701/1‑101 (MQ=255)
                                                                                ggggCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAAc                      >  1:4314812/1‑101 (MQ=255)
                                                                                  ggCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACgg                    >  1:2015004/1‑101 (MQ=255)
                                                                                    cGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCg                  >  2:4170146/1‑101 (MQ=255)
                                                                                     gTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGt                 >  1:2509317/1‑101 (MQ=255)
                                                                                      tGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTc                >  2:1548726/1‑101 (MQ=255)
                                                                                       ggATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa               >  1:1915049/1‑101 (MQ=255)
                                                                                       ggATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa               >  1:771139/1‑101 (MQ=255)
                                                                                       ggATCGAAACGTGCCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa               >  2:3689611/1‑101 (MQ=255)
                                                                                         aTCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGCAACACCGGGCACGCGCGAACGGCGTCAGc             >  2:3891448/1‑101 (MQ=255)
                                                                                          tCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcc            <  1:2037716/101‑1 (MQ=255)
                                                                                           cGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc           >  1:2729149/1‑101 (MQ=255)
                                                                                           cGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc           >  1:598870/1‑101 (MQ=255)
                                                                                           cGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc           >  1:1782861/1‑101 (MQ=255)
                                                                                            gAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc          <  1:917770/101‑1 (MQ=255)
                                                                                            gAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc          <  2:1942844/101‑1 (MQ=255)
                                                                                            gAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc          <  1:297238/101‑1 (MQ=255)
                                                                                            gAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc          >  2:2020094/1‑101 (MQ=255)
                                                                                             aaaCGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcg         >  2:3430653/1‑101 (MQ=255)
                                                                                               aCGTGTCTGCCA.CGAC.GCCCTGCACTAGATACGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACACCGCGCACGCGCGCACCGCCTCAGCCCcgcg       >  2:3257089/1‑101 (MQ=37)
                                                                                                 gtgtCTGCCC.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa     <  1:2640068/101‑1 (MQ=255)
                                                                                                 gtgtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa     >  1:1002314/1‑101 (MQ=255)
                                                                                                  tgtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGCAc    >  2:1055669/1‑101 (MQ=255)
                                                                                                  tgtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc    <  2:4314565/101‑1 (MQ=255)
                                                                                                  tgtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc    <  2:2115297/101‑1 (MQ=255)
                                                                                                    |                                                                                                       
CGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGT  >  NC_014034/1397102‑1397302

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG