Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,206 C→A 4.1% A363D (GCC→GAC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2060C→A 4.1% 10.8 ‑2.5 ‑0.0215A363D (GCC→GAC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/7):  ref base (116/89):  total (119/96)
Fisher's exact test strand distribution p-value = 8.16e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.96e-01

Only 100 of 232 total aligned reads displayed.

CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCT  >  NC_014034/1397106‑1397306
                                                                                                    |                                                                                                       
ccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGc                                                                                                         >  1:61112/1‑101 (MQ=255)
 cccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGcc                                                                                                        >  1:4462649/1‑101 (MQ=255)
  ccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa                                                                                                       <  1:3918276/101‑1 (MQ=255)
  ccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa                                                                                                       <  1:3969135/101‑1 (MQ=255)
   cccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.c                                                                                                     <  1:4357688/101‑1 (MQ=255)
      gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cgac                                                                                                  <  1:381051/101‑1 (MQ=255)
      gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cgac                                                                                                  <  2:2519793/101‑1 (MQ=255)
      gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cgac                                                                                                  <  2:3666263/101‑1 (MQ=255)
       cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGTGTCTGCCa.ggac.g                                                                                                >  2:1862141/1‑101 (MQ=255)
        gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.Gc                                                                                               <  1:118147/101‑1 (MQ=255)
        gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.Gc                                                                                               >  2:3415694/1‑101 (MQ=255)
           ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.Gcccc                                                                                            >  1:957424/1‑100 (MQ=255)
           ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCt                                                                                            <  1:1311959/101‑1 (MQ=255)
           ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCt                                                                                            <  1:3385895/101‑1 (MQ=255)
           ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCt                                                                                            <  1:2861539/101‑1 (MQ=255)
            gCGTGGATCGAAACGTCCAGCTCGCGTCACATGTCTTCGTCCGTGCTCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTg                                                                                           <  2:3918664/101‑1 (MQ=37)
            gCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTg                                                                                           <  1:4641611/101‑1 (MQ=255)
              gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGCC.GCCCTGCa                                                                                         >  1:4499073/1‑101 (MQ=255)
              gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCa                                                                                         >  2:2660611/1‑101 (MQ=255)
              gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCa                                                                                         >  2:3526301/1‑101 (MQ=255)
               tGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCAc                                                                                        >  1:2083728/1‑101 (MQ=255)
                ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCAcc                                                                                       >  1:2757611/1‑101 (MQ=255)
                ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCAcc                                                                                       <  1:3450517/101‑1 (MQ=255)
                 gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCa                                                                                      >  2:1115508/1‑101 (MQ=255)
                  aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAg                                                                                     <  1:2660765/101‑1 (MQ=255)
                   tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGa                                                                                    >  1:3711049/1‑101 (MQ=255)
                   tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGa                                                                                    <  2:4563672/101‑1 (MQ=255)
                   tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGa                                                                                    >  2:2545050/1‑101 (MQ=255)
                    cGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGAt                                                                                   <  2:2274331/101‑1 (MQ=255)
                     gAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATc                                                                                  >  1:3232409/1‑101 (MQ=255)
                     gAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATc                                                                                  >  2:1782988/1‑101 (MQ=255)
                     gAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATc                                                                                  >  1:489581/1‑101 (MQ=255)
                      aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATcc                                                                                 >  2:4335975/1‑101 (MQ=255)
                      aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATcc                                                                                 >  2:3037254/1‑101 (MQ=255)
                      aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATcc                                                                                 <  1:2165766/101‑1 (MQ=255)
                       aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCcg                                                                                >  1:1916182/1‑101 (MQ=255)
                            ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.g                                                                          <  2:61105/101‑1 (MQ=255)
                             cAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.Gt                                                                         <  1:4625212/101‑1 (MQ=255)
                                    cgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTccc                                                                  >  1:1900238/1‑101 (MQ=255)
                                         caTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcgc                                                             <  1:2016102/101‑1 (MQ=255)
                                           tGTCTTCGACCGTGCGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCgg                                                           <  1:1636604/101‑1 (MQ=255)
                                            gTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCggg                                                          >  1:3634401/1‑101 (MQ=255)
                                             tCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCgggg                                                         >  2:1182041/1‑101 (MQ=255)
                                               ttCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGc                                                       >  2:2639914/1‑101 (MQ=255)
                                               ttCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGc                                                       >  1:3052490/1‑101 (MQ=255)
                                                 cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCgg                                                     >  2:75198/1‑100 (MQ=255)
                                                 cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGt                                                     >  1:2388142/1‑101 (MQ=255)
                                                     cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                 <  1:1444410/101‑1 (MQ=255)
                                                     cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                 >  1:429603/1‑101 (MQ=255)
                                                      gTGCGCCGGCGCCCTCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGTGGCGTGGATc                                                <  1:4099684/101‑1 (MQ=38)
                                                       tGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                               >  1:186109/1‑101 (MQ=255)
                                                        gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCC.AACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                               <  1:916041/101‑1 (MQ=2)
                                                        gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCC.AACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                               <  1:4562464/101‑1 (MQ=2)
                                                        gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCC.AACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                               <  1:1591119/101‑1 (MQ=2)
                                                        cgatcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGC.TGGC.GGCCCGGTCGTGAATCTCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                               >  1:4062802/5‑101 (MQ=2)
                                                         tatcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGC.TTGACGCGCTGGATCCGCGGC.GCCGCTCCCCCCGCGGGGGCGTGGATCGa                                              >  1:3204046/4‑101 (MQ=2)
                                                           gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCC.AACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                            <  2:337615/100‑1 (MQ=2)
                                                           ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                           <  1:1115594/101‑1 (MQ=255)
                                                            cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                          >  2:305714/1‑101 (MQ=255)
                                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                         >  1:314792/1‑101 (MQ=255)
                                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                         <  2:3728638/101‑1 (MQ=255)
                                                             gTCGCTCCCCCCGCGGGGGCGTGGACCGAAACCTATGAGGGG.TGGCGGCCCGGTCGTGGATCC.CGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                          <  1:1963394/101‑1 (MQ=0)
                                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAcc                                        >  2:4663004/1‑101 (MQ=255)
                                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                      >  2:4382313/1‑101 (MQ=255)
                                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                      <  2:793657/101‑1 (MQ=255)
                                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                      >  1:824799/1‑101 (MQ=255)
                                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgcg                                     >  2:3715565/1‑101 (MQ=255)
                                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgcg                                     >  1:730182/1‑101 (MQ=255)
                                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACGCGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgcg                                     >  1:3341568/1‑101 (MQ=255)
                                                                   ccccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCa                                   >  2:3533596/1‑101 (MQ=255)
                                                                   ccccccGCGGGGGCGTGGATCCAAACGTTTCTGCCA.CGAC.GCCCTGCACCAGCTCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCa                                   <  1:3577386/101‑1 (MQ=255)
                                                                    cccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGTCGTGGATCGAAACACCGCGCAc                                  <  2:688099/101‑1 (MQ=255)
                                                                      cccGGGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgc                                <  2:586130/101‑1 (MQ=255)
                                                                          cgGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGa                            <  2:2715843/101‑1 (MQ=255)
                                                                            ggggCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAAc                          >  2:3137701/1‑101 (MQ=255)
                                                                            ggggCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCCCACGCCCGAAc                          >  2:1755474/1‑101 (MQ=255)
                                                                              ggCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACgg                        <  2:1333082/101‑1 (MQ=255)
                                                                              ggCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACgg                        <  2:3232208/101‑1 (MQ=255)
                                                                                cGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCg                      >  2:4170146/1‑101 (MQ=255)
                                                                                cGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCg                      >  2:458698/1‑101 (MQ=255)
                                                                                  tGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTc                    >  2:1548726/1‑101 (MQ=255)
                                                                                   ggATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                   >  1:2314321/1‑101 (MQ=255)
                                                                                     aTCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGCAACACCGGGCACGCGCGAACGGCGTCAGc                 >  2:3891448/1‑101 (MQ=255)
                                                                                       cGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc               >  1:1782861/1‑101 (MQ=255)
                                                                                       cGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc               >  1:598870/1‑101 (MQ=255)
                                                                                        gAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc              <  1:297238/101‑1 (MQ=255)
                                                                                        gAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc              <  2:4313285/101‑1 (MQ=255)
                                                                                        gAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc              <  2:1942844/101‑1 (MQ=255)
                                                                                        gAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc              >  2:2020094/1‑101 (MQ=255)
                                                                                         aaaCGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGCCAGccccc             >  1:2404391/1‑100 (MQ=255)
                                                                                           aCGTGTCTGCCA.CGAC.GCCCTGCACTAGATACGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACACCGCGCACGCGCGCACCGCCTCAGCCCcgcg           >  2:3257089/1‑101 (MQ=37)
                                                                                             gtgtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa         >  1:2584528/1‑101 (MQ=255)
                                                                                             gtgtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa         >  1:1002314/1‑101 (MQ=255)
                                                                                               gtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACg       <  1:2545195/101‑1 (MQ=255)
                                                                                                tCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGt      >  2:1606953/1‑101 (MQ=255)
                                                                                                 cTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTc     <  2:1635918/101‑1 (MQ=255)
                                                                                                  tGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCg    >  1:1557235/1‑101 (MQ=255)
                                                                                                   gCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGc   >  2:2075211/1‑101 (MQ=255)
                                                                                                    ccA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCt  >  2:4212284/1‑101 (MQ=255)
                                                                                                    ccA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCt  >  2:905689/1‑101 (MQ=255)
                                                                                                    |                                                                                                       
CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCT  >  NC_014034/1397106‑1397306

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG