Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,211 A→C 4.0% T365P (ACG→CCA)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2110A→C 4.0% 11.5 ‑3.9 ‑0.1211T365P (ACG→CCG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/8):  ref base (117/82):  total (121/90)
Fisher's exact test strand distribution p-value = 2.10e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.37e-01

Only 100 of 230 total aligned reads displayed.

GCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCC  >  NC_014034/1397112‑1397311
                                                                                                    |                                                                                                      
gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cgac                                                                                                       <  2:2519793/101‑1 (MQ=255)
gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cgac                                                                                                       <  2:3666263/101‑1 (MQ=255)
 cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cgac.g                                                                                                     <  1:556297/101‑1 (MQ=255)
  gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.Gc                                                                                                    >  2:3415694/1‑101 (MQ=255)
  gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.Gc                                                                                                    <  1:118147/101‑1 (MQ=255)
     ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCt                                                                                                 <  2:1719867/101‑1 (MQ=255)
     ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCt                                                                                                 <  1:1311959/101‑1 (MQ=255)
      gCGTGGATCGAAACGTCCAGCTCGCGTCACATGTCTTCGTCCGTGCTCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTg                                                                                                <  2:3918664/101‑1 (MQ=37)
        gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCa                                                                                              >  2:2660611/1‑101 (MQ=255)
         tGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCAc                                                                                             >  1:2083728/1‑101 (MQ=255)
           gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCa                                                                                           <  2:306185/101‑1 (MQ=255)
           gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCa                                                                                           >  2:1115508/1‑101 (MQ=255)
            aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAg                                                                                          <  1:2660765/101‑1 (MQ=255)
             tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGa                                                                                         <  2:4563672/101‑1 (MQ=255)
              cGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGAt                                                                                        >  1:2836173/1‑101 (MQ=255)
               gAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATc                                                                                       >  2:1782988/1‑101 (MQ=255)
               gAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATc                                                                                       <  1:388861/101‑1 (MQ=255)
                aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATcc                                                                                      <  1:2165766/101‑1 (MQ=255)
                aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATcc                                                                                      <  1:3146884/101‑1 (MQ=255)
                 aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCcg                                                                                     >  1:1916182/1‑101 (MQ=255)
                  aCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCcgc                                                                                    >  1:2242446/1‑101 (MQ=255)
                    gTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCgcgc                                                                                  >  2:243482/1‑101 (MQ=255)
                      ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.g                                                                               <  2:61105/101‑1 (MQ=255)
                       cAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.Gt                                                                              <  1:4625212/101‑1 (MQ=255)
                        aGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTc                                                                             <  2:3531063/101‑1 (MQ=255)
                        aGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTc                                                                             >  2:3489031/1‑101 (MQ=255)
                        aGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTc                                                                             <  2:3911822/101‑1 (MQ=255)
                         gCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCg                                                                            >  1:4313532/1‑101 (MQ=255)
                              cgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTccc                                                                       <  1:3240935/101‑1 (MQ=255)
                              cgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTccc                                                                       >  1:1900238/1‑101 (MQ=255)
                               gcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTcccc                                                                      <  2:3048076/101‑1 (MQ=255)
                                     tGTCTTCGACCGTGCGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCgg                                                                <  1:1636604/101‑1 (MQ=255)
                                      gTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCggg                                                               >  1:3634401/1‑101 (MQ=255)
                                       tCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCgggg                                                              >  2:1182041/1‑101 (MQ=255)
                                        cTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGTCTGCCA.CGAC.GGCCTCCACCATATCCGCGGC.GCCGCTCCCCCCGCggggg                                                             >  2:3717516/1‑101 (MQ=37)
                                         ttCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGc                                                            >  2:2639914/1‑101 (MQ=255)
                                          tCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCg                                                           >  1:4693898/1‑101 (MQ=255)
                                           cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCgg                                                          >  2:75198/1‑100 (MQ=255)
                                           cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGt                                                          >  1:2388142/1‑101 (MQ=255)
                                           cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCgg                                                          >  1:4487708/1‑100 (MQ=255)
                                            gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAA.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTg                                                         >  2:1876260/1‑101 (MQ=255)
                                             gccGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTgg                                                        <  1:3037442/100‑1 (MQ=255)
                                               cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                      <  1:1444410/101‑1 (MQ=255)
                                               cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                      <  1:3526513/101‑1 (MQ=255)
                                                 tGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    >  1:186109/1‑101 (MQ=255)
                                                  gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCC.AACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    <  1:916041/101‑1 (MQ=2)
                                                  gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCC.AACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    <  1:1591119/101‑1 (MQ=2)
                                                  gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCC.AACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    <  1:4562464/101‑1 (MQ=2)
                                                   tatcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGC.TTGACGCGCTGGATCCGCGGC.GCCGCTCCCCCCGCGGGGGCGTGGATCGa                                                   >  1:3204046/4‑101 (MQ=2)
                                                     gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCC.AACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 <  2:2727066/100‑1 (MQ=2)
                                                     ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                >  1:3444015/1‑101 (MQ=255)
                                                     ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                <  1:1115594/101‑1 (MQ=255)
                                                      cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                               <  1:4213506/101‑1 (MQ=255)
                                                       gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                              <  2:2242322/101‑1 (MQ=255)
                                                       gTCGCTCCCCCCGCGGGGGCGTGGACCGAAACCTATGAGGGG.TGGCGGCCCGGTCGTGGATCC.CGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                               <  1:1963394/101‑1 (MQ=0)
                                                        tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAcc                                             >  2:4663004/1‑101 (MQ=255)
                                                        tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GACGCTCCCCCCGCGGGGGCGTGGATCGAAACAcc                                             >  1:4071832/1‑101 (MQ=255)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                           >  1:824799/1‑101 (MQ=255)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                           <  2:793657/101‑1 (MQ=255)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgcg                                          >  1:730182/1‑101 (MQ=255)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACGCGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgcg                                          >  1:3341568/1‑101 (MQ=255)
                                                             ccccccGCGGGGGCGTGGATCCAAACGTTTCTGCCA.CGAC.GCCCTGCACCAGCTCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCa                                        <  1:3577386/101‑1 (MQ=255)
                                                              cccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGTCGTGGATCGAAACACCGCGCAc                                       <  2:688099/101‑1 (MQ=255)
                                                              cccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAc                                       <  2:1451917/101‑1 (MQ=255)
                                                                cccGGGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgc                                     <  2:586130/101‑1 (MQ=255)
                                                                  cgcgGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgcgc                                   <  2:4462399/101‑1 (MQ=255)
                                                                    cgGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGa                                 >  2:3706405/1‑101 (MQ=255)
                                                                    cgGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGa                                 <  2:2715843/101‑1 (MQ=255)
                                                                    cgGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGCATCGAAACAACCCGGCcgcgcgc                                 >  2:4235612/1‑100 (MQ=37)
                                                                      ggggCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCCCACGCCCGAAc                               >  2:1755474/1‑101 (MQ=255)
                                                                       gggCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACg                              >  2:3753936/1‑101 (MQ=255)
                                                                        ggCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACgg                             <  2:3232208/101‑1 (MQ=255)
                                                                        ggCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACgg                             >  1:2015004/1‑101 (MQ=255)
                                                                          cGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCg                           >  2:2052277/1‑101 (MQ=255)
                                                                          cGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCg                           >  2:4170146/1‑101 (MQ=255)
                                                                          cGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCg                           >  2:458698/1‑101 (MQ=255)
                                                                           gTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGt                          >  1:2509317/1‑101 (MQ=255)
                                                                            tGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTc                         >  2:1548726/1‑101 (MQ=255)
                                                                             ggATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGAGCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                        <  2:2576199/101‑1 (MQ=255)
                                                                                 cGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc                    >  1:1782861/1‑101 (MQ=255)
                                                                                 cGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc                    >  1:2729149/1‑101 (MQ=255)
                                                                                  gAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                   <  1:297238/101‑1 (MQ=255)
                                                                                  gAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                   <  1:602104/101‑1 (MQ=255)
                                                                                  gAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                   <  2:1942844/101‑1 (MQ=255)
                                                                                   aaaCGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGCCAGccccc                  >  1:2404391/1‑100 (MQ=255)
                                                                                     aCGTGTCTGCCA.CGAC.GCCCTGCACTAGATACGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACACCGCGCACGCGCGCACCGCCTCAGCCCcgcg                >  2:3257089/1‑101 (MQ=37)
                                                                                       gtgtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa              >  1:2584528/1‑101 (MQ=255)
                                                                                       gtgtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa              >  1:1002314/1‑101 (MQ=255)
                                                                                        tgtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGCAc             >  2:1055669/1‑101 (MQ=255)
                                                                                        tgtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc             <  2:4314565/101‑1 (MQ=255)
                                                                                        tgtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc             >  1:4527640/1‑101 (MQ=255)
                                                                                        tgtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc             >  1:3748118/1‑101 (MQ=255)
                                                                                          tCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGt           >  2:1606953/1‑101 (MQ=255)
                                                                                            tGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCg         >  2:4369188/1‑101 (MQ=255)
                                                                                            tGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCg         >  1:1557235/1‑101 (MQ=255)
                                                                                             gCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGc        <  1:3975146/101‑1 (MQ=255)
                                                                                              ccA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCt       >  2:905689/1‑101 (MQ=255)
                                                                                                 ccgac.gCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcg     <  2:1986719/100‑2 (MQ=255)
                                                                                                   gac.gCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccc   >  2:3355915/1‑101 (MQ=255)
                                                                                                   gac.gCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccc   >  2:1236384/1‑101 (MQ=255)
                                                                                                    |                                                                                                      
GCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCC  >  NC_014034/1397112‑1397311

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG