Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,216 C→A 3.7% P366P (CCC→CCA RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2160C→A 3.7% 8.6 ‑3.8 ‑0.2216P366P (CCC→CCARCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/7):  ref base (119/89):  total (120/96)
Fisher's exact test strand distribution p-value = 2.33e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.79e-01

Only 100 of 228 total aligned reads displayed.

GGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGG  >  NC_014034/1397117‑1397316
                                                                                                     |                                                                                                     
ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.Gcccc                                                                                                      >  1:957424/1‑100 (MQ=255)
ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCt                                                                                                      <  1:2861539/101‑1 (MQ=255)
ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCt                                                                                                      <  2:1719867/101‑1 (MQ=255)
 gCGTGGATCGAAACGTCCAGCTCGCGTCACATGTCTTCGTCCGTGCTCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTg                                                                                                     <  2:3918664/101‑1 (MQ=37)
     ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCAcc                                                                                                 >  1:2757611/1‑101 (MQ=255)
      gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCa                                                                                                <  1:4628425/101‑1 (MQ=255)
      gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCa                                                                                                <  1:2393703/101‑1 (MQ=255)
       aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAg                                                                                               <  1:212568/101‑1 (MQ=255)
        tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGa                                                                                              <  2:4563672/101‑1 (MQ=255)
          gAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATc                                                                                            >  2:1782988/1‑101 (MQ=255)
          gAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATc                                                                                            <  1:388861/101‑1 (MQ=255)
           aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATcc                                                                                           >  1:1452011/1‑101 (MQ=255)
           aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATcc                                                                                           >  2:3037254/1‑101 (MQ=255)
           aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATcc                                                                                           <  1:3146884/101‑1 (MQ=255)
           aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAGTCTGCCA.CGAC.GCCCTGCACCAGATcc                                                                                           >  2:2650/1‑101 (MQ=255)
            aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCcg                                                                                          >  1:1916182/1‑101 (MQ=255)
               gTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCgcgc                                                                                       >  2:243482/1‑101 (MQ=255)
                 ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.g                                                                                    <  2:61105/101‑1 (MQ=255)
                   aGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTc                                                                                  <  2:3911822/101‑1 (MQ=255)
                   aGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTc                                                                                  <  2:3531063/101‑1 (MQ=255)
                    gCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCg                                                                                 >  1:4313532/1‑101 (MQ=255)
                         cgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTccc                                                                            <  1:3240935/101‑1 (MQ=255)
                           ccACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTccccc                                                                          <  2:2529536/101‑1 (MQ=255)
                              caTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcgc                                                                       <  1:3562592/101‑1 (MQ=255)
                               aTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcgcg                                                                      >  1:4573901/1‑101 (MQ=255)
                                tGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCgg                                                                     <  2:2621619/101‑1 (MQ=255)
                                tGTCTTCGACCGTGCGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCgg                                                                     <  1:1636604/101‑1 (MQ=255)
                                   cTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGTCTGCCA.CGAC.GGCCTCCACCATATCCGCGGC.GCCGCTCCCCCCGCggggg                                                                  >  2:3717516/1‑101 (MQ=37)
                                    ttCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGc                                                                 >  1:3052490/1‑101 (MQ=255)
                                     tCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCg                                                                >  1:4693898/1‑101 (MQ=255)
                                     tCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCg                                                                <  1:3698931/101‑1 (MQ=255)
                                      cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCgg                                                               >  2:75198/1‑100 (MQ=255)
                                      cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCgg                                                               >  1:4487708/1‑100 (MQ=255)
                                           gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                          >  2:3598571/1‑101 (MQ=255)
                                           gTGCGCCGGCGCCCTCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGTGGCGTGGATc                                                          <  1:4099684/101‑1 (MQ=38)
                                            tGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         >  1:186109/1‑101 (MQ=255)
                                            ggcgcCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         <  1:3362447/100‑1 (MQ=255)
                                             gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCC.AACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         <  1:916041/101‑1 (MQ=2)
                                             gcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                        <  1:1250738/101‑1 (MQ=255)
                                             cgatcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGC.TGGC.GGCCCGGTCGTGAATCTCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         >  1:4062802/5‑101 (MQ=2)
                                              cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCC.ACCAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                        >  1:1880939/4‑101 (MQ=2)
                                               gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTGTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCGCCCCCCGCGGGGGCGTGGATCGaaa                                                      <  1:3196984/101‑1 (MQ=255)
                                                gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCC.AACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                      <  1:2221811/100‑1 (MQ=2)
                                                gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCC.AACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                      <  2:337615/100‑1 (MQ=2)
                                                ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                     >  1:3444015/1‑101 (MQ=255)
                                                ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                     <  1:1115594/101‑1 (MQ=255)
                                                 cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                    >  2:305714/1‑101 (MQ=255)
                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                   <  2:2242322/101‑1 (MQ=255)
                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                   >  1:3324558/1‑101 (MQ=255)
                                                  gTCGCTCCCCCCGCGGGGGCGTGGACCGAAACCTATGAGGGG.TGGCGGCCCGGTCGTGGATCC.CGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                    <  1:1963394/101‑1 (MQ=0)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                                <  2:793657/101‑1 (MQ=255)
                                                        ccccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCa                                             >  2:115182/1‑101 (MQ=255)
                                                         cccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGTCGTGGATCGAAACACCGCGCAc                                            <  2:688099/101‑1 (MQ=255)
                                                           cccGGGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgc                                          <  2:586130/101‑1 (MQ=255)
                                                             cgcgGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgcgc                                        <  2:4462399/101‑1 (MQ=255)
                                                               cgGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGa                                      <  2:2715843/101‑1 (MQ=255)
                                                               cgGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGa                                      >  2:3706405/1‑101 (MQ=255)
                                                               cgGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGCATCGAAACAACCCGGCcgcgcgc                                      >  2:4235612/1‑100 (MQ=37)
                                                                 ggggCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAAc                                    >  2:3137701/1‑101 (MQ=255)
                                                                 ggggCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCCCACGCCCGAAc                                    >  2:1755474/1‑101 (MQ=255)
                                                                  gggCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACg                                   >  2:1097920/1‑101 (MQ=255)
                                                                   ggCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACgg                                  >  1:2015004/1‑101 (MQ=255)
                                                                   ggCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACgg                                  <  2:1333082/101‑1 (MQ=255)
                                                                     cGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCg                                >  2:2052277/1‑101 (MQ=255)
                                                                     cGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCg                                >  2:458698/1‑101 (MQ=255)
                                                                        ggATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                             >  1:771139/1‑101 (MQ=255)
                                                                        ggATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                             >  1:2314321/1‑101 (MQ=255)
                                                                         gATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAg                            >  2:470730/1‑101 (MQ=255)
                                                                          aTCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGCAACACCGGGCACGCGCGAACGGCGTCAGc                           >  2:3891448/1‑101 (MQ=255)
                                                                          aTCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGc                           >  1:1330050/1‑101 (MQ=255)
                                                                          aTCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGc                           >  2:3293997/1‑101 (MQ=255)
                                                                           tCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcc                          <  2:1506648/101‑1 (MQ=255)
                                                                            cGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc                         >  1:1782861/1‑101 (MQ=255)
                                                                             gAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                        >  1:2989686/1‑101 (MQ=255)
                                                                             gAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                        >  1:665180/1‑101 (MQ=255)
                                                                             gAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                        <  1:602104/101‑1 (MQ=255)
                                                                             gAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                        >  1:2441289/1‑101 (MQ=255)
                                                                             gAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                        <  2:1942844/101‑1 (MQ=255)
                                                                              aaaCGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcg                       >  2:3430653/1‑101 (MQ=255)
                                                                                aCGTGTCTGCCA.CGCC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcgcg                     <  1:1577406/101‑1 (MQ=255)
                                                                                  gtgtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa                   >  1:1002314/1‑101 (MQ=255)
                                                                                  gtgtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa                   >  1:1595819/1‑101 (MQ=255)
                                                                                  gtgtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa                   >  1:2584528/1‑101 (MQ=255)
                                                                                  gtgtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa                   >  1:3590343/1‑101 (MQ=255)
                                                                                   tgtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc                  <  2:4314565/101‑1 (MQ=255)
                                                                                    gtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACg                 >  2:1876408/1‑101 (MQ=255)
                                                                                    gtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACg                 <  1:2545195/101‑1 (MQ=255)
                                                                                     tCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGt                >  2:1606953/1‑101 (MQ=255)
                                                                                       tGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCg              >  2:3247741/1‑101 (MQ=255)
                                                                                       tGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCg              >  1:4537357/1‑101 (MQ=255)
                                                                                       tGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCg              >  2:4369188/1‑101 (MQ=255)
                                                                                        gCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGc             <  1:3975146/101‑1 (MQ=255)
                                                                                         ccA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCt            >  2:905689/1‑101 (MQ=255)
                                                                                         ccA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCt            >  2:4212284/1‑101 (MQ=255)
                                                                                         ccA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCCCt            <  1:2715082/101‑1 (MQ=255)
                                                                                          cA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTc           <  2:2696712/101‑1 (MQ=255)
                                                                                            ccgac.gCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcg          <  2:1986719/100‑2 (MQ=255)
                                                                                              gac.gCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccc        >  2:1236384/1‑101 (MQ=255)
                                                                                                c.gCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccccc      <  1:2020211/101‑1 (MQ=255)
                                                                                                  gCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcg     <  1:4369435/101‑1 (MQ=255)
                                                                                                     |                                                                                                     
GGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGG  >  NC_014034/1397117‑1397316

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG