Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,217 T→A 3.8% C367S (TGC→AAT)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2170T→A 3.8% 13.9 ‑3.8 ‑0.3211C367S (TGC→AGC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/7):  ref base (117/85):  total (119/92)
Fisher's exact test strand distribution p-value = 2.27e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.74e-01

Only 100 of 233 total aligned reads displayed.

GGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGG  >  NC_014034/1397117‑1397317
                                                                                                      |                                                                                                     
ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCt                                                                                                       <  1:3385895/101‑1 (MQ=255)
ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCt                                                                                                       >  2:2714928/1‑101 (MQ=255)
ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCt                                                                                                       <  1:2861539/101‑1 (MQ=255)
 gCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTg                                                                                                      <  1:4641611/101‑1 (MQ=255)
   gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGCC.GCCCTGCa                                                                                                    >  1:4499073/1‑101 (MQ=255)
     ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCAcc                                                                                                  <  1:3450517/101‑1 (MQ=255)
     ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCAcc                                                                                                  >  1:2757611/1‑101 (MQ=255)
      gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCa                                                                                                 <  2:306185/101‑1 (MQ=255)
       aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAg                                                                                                <  1:212568/101‑1 (MQ=255)
        tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGa                                                                                               >  1:3711049/1‑101 (MQ=255)
        tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGa                                                                                               >  2:2545050/1‑101 (MQ=255)
         cGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGAt                                                                                              <  2:2274331/101‑1 (MQ=255)
          gAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATc                                                                                             <  1:388861/101‑1 (MQ=255)
          gAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATc                                                                                             >  2:1782988/1‑101 (MQ=255)
           aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATcc                                                                                            >  2:4335975/1‑101 (MQ=255)
           aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATcc                                                                                            >  1:1452011/1‑101 (MQ=255)
           aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATcc                                                                                            >  2:3037254/1‑101 (MQ=255)
           aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATcc                                                                                            <  1:3146884/101‑1 (MQ=255)
           aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAGTCTGCCA.CGAC.GCCCTGCACCAGATcc                                                                                            >  2:2650/1‑101 (MQ=255)
            aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCcg                                                                                           >  1:1916182/1‑101 (MQ=255)
               gTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCgcgc                                                                                        >  2:243482/1‑101 (MQ=255)
               gTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCgcgc                                                                                        >  2:4624959/1‑101 (MQ=255)
                  cAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.Gt                                                                                    <  1:4625212/101‑1 (MQ=255)
                   aGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTc                                                                                   >  2:3489031/1‑101 (MQ=255)
                         cgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTccc                                                                             >  1:1900238/1‑101 (MQ=255)
                         cgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTccc                                                                             <  1:3240935/101‑1 (MQ=255)
                            cacaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTcccccc                                                                          <  2:408907/101‑1 (MQ=255)
                              caTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcgc                                                                        <  1:3562592/101‑1 (MQ=255)
                              caTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcgc                                                                        <  1:2016102/101‑1 (MQ=255)
                               aTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcgcg                                                                       >  2:1265797/1‑101 (MQ=255)
                                tGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCgg                                                                      <  2:2621619/101‑1 (MQ=255)
                                 gTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCggg                                                                     >  1:3634401/1‑101 (MQ=255)
                                    ttCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGc                                                                  >  1:3052490/1‑101 (MQ=255)
                                      cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGt                                                                >  1:2388142/1‑101 (MQ=255)
                                       gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAA.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTg                                                               >  2:1876260/1‑101 (MQ=255)
                                         ccGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                             >  2:602040/1‑101 (MQ=255)
                                          cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCTGCGGGGGCGTGGAt                                                            >  2:3138702/1‑101 (MQ=255)
                                            tGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                          >  1:186109/1‑101 (MQ=255)
                                             gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCC.AACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                          <  1:916041/101‑1 (MQ=2)
                                             gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCC.AACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                          <  1:4562464/101‑1 (MQ=2)
                                             gcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                         <  1:1250738/101‑1 (MQ=255)
                                              tatcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGC.TTGACGCGCTGGATCCGCGGC.GCCGCTCCCCCCGCGGGGGCGTGGATCGa                                                         >  1:3204046/4‑101 (MQ=2)
                                              cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                        <  2:271733/101‑1 (MQ=255)
                                              cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                        <  2:757506/101‑1 (MQ=255)
                                                gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCC.AACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                       <  1:2221811/100‑1 (MQ=2)
                                                gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCC.AACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                       <  1:4392604/100‑1 (MQ=2)
                                                ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                      <  1:1115594/101‑1 (MQ=255)
                                                 cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                     <  2:3914230/101‑1 (MQ=255)
                                                 cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                     <  1:4213506/101‑1 (MQ=255)
                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                    >  1:3324558/1‑101 (MQ=255)
                                                  gTCGCTCCCCCCGCGGGGGCGTGGACCGAAACCTATGAGGGG.TGGCGGCCCGGTCGTGGATCC.CGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                     <  1:1963394/101‑1 (MQ=0)
                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GACGCTCCCCCCGCGGGGGCGTGGATCGAAACAcc                                                   >  1:4071832/1‑101 (MQ=255)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                                 >  1:824799/1‑101 (MQ=255)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                                 >  2:636134/1‑101 (MQ=255)
                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgcg                                                >  2:3715565/1‑101 (MQ=255)
                                                        ccccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCa                                              >  2:115182/1‑101 (MQ=255)
                                                        ccccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCa                                              >  2:3533596/1‑101 (MQ=255)
                                                         cccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAc                                             >  1:4008530/1‑101 (MQ=255)
                                                             cgcgGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgcgc                                         <  2:4462399/101‑1 (MQ=255)
                                                               cgGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGa                                       >  2:3392251/1‑101 (MQ=255)
                                                               cgGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGCATCGAAACAACCCGGCcgcgcgc                                       >  2:4235612/1‑100 (MQ=37)
                                                                gggggCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGaa                                      <  2:4573648/101‑1 (MQ=255)
                                                                 ggggCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAAc                                     >  1:4314812/1‑101 (MQ=255)
                                                                 ggggCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCCCACGCCCGAAc                                     >  2:1755474/1‑101 (MQ=255)
                                                                  gggCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACg                                    >  2:3753936/1‑101 (MQ=255)
                                                                        ggATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                              >  1:2314321/1‑101 (MQ=255)
                                                                        ggATCGAAACGTGCCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                              >  2:3689611/1‑101 (MQ=255)
                                                                         gATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAg                             >  2:470730/1‑101 (MQ=255)
                                                                          aTCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGc                            >  1:1330050/1‑101 (MQ=255)
                                                                           tCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcc                           <  1:2037716/101‑1 (MQ=255)
                                                                           tCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcc                           <  2:1506648/101‑1 (MQ=255)
                                                                            cGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc                          >  1:1782861/1‑101 (MQ=255)
                                                                            cGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc                          >  1:598870/1‑101 (MQ=255)
                                                                             gAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                         >  2:2020094/1‑101 (MQ=255)
                                                                             gAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                         <  2:1942844/101‑1 (MQ=255)
                                                                             gAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                         >  1:2989686/1‑101 (MQ=255)
                                                                             gAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                         >  1:2441289/1‑101 (MQ=255)
                                                                              aaaCGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcg                        >  2:719221/1‑101 (MQ=255)
                                                                                aCGTGTCTGCCA.CGCC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcgcg                      <  1:1577406/101‑1 (MQ=255)
                                                                                  gtgtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa                    >  1:1002314/1‑101 (MQ=255)
                                                                                  gtgtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa                    >  1:1595819/1‑101 (MQ=255)
                                                                                  gtgtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa                    >  1:2584528/1‑101 (MQ=255)
                                                                                   tgtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGCAc                   >  2:1055669/1‑101 (MQ=255)
                                                                                   tgtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc                   <  2:2115297/101‑1 (MQ=255)
                                                                                    gtCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACg                  <  1:2545195/101‑1 (MQ=255)
                                                                                     tCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGt                 >  2:1606953/1‑101 (MQ=255)
                                                                                     tCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGt                 <  1:636198/101‑1 (MQ=255)
                                                                                      cTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTc                <  2:1635918/101‑1 (MQ=255)
                                                                                       taacA.CGAA.GCCCTGCACCAGATCCGTGCC.GTCGCCTCCGCCGCGGAGGCGCTGCTCCCACGACCGCGCACGCGCGTAGCGCGGCAGTCCCCCGAACGCCg               >  2:3901043/4‑101 (MQ=255)
                                                                                       tGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCg               >  2:3247741/1‑101 (MQ=255)
                                                                                         ccA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCt             >  2:905689/1‑101 (MQ=255)
                                                                                         ccA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCt             >  2:4212284/1‑101 (MQ=255)
                                                                                         ccA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCCCt             <  1:2715082/101‑1 (MQ=255)
                                                                                            ccgac.gCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcg           <  2:1986719/100‑2 (MQ=255)
                                                                                              gac.gCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccc         <  2:4381541/101‑1 (MQ=255)
                                                                                              gac.gCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccc         >  2:3355915/1‑101 (MQ=255)
                                                                                               ac.gCCCTGCCCCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTccccc        <  2:120558/101‑1 (MQ=255)
                                                                                                c.gCCCTGCCCCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccccc       <  1:1707887/101‑1 (MQ=255)
                                                                                                      tGCACCAGATCCGCGCT.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCggg  >  1:723438/1‑101 (MQ=255)
                                                                                                      tGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCggg  <  2:824726/101‑1 (MQ=255)
                                                                                                      |                                                                                                     
GGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAC.GCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGG  >  NC_014034/1397117‑1397317

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG