Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,221 C→T 3.8% T368I (ACC→ATG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2210C→T 3.8% 12.7 ‑3.8 ‑0.0212T368I (ACC→ATC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/7):  ref base (117/87):  total (118/94)
Fisher's exact test strand distribution p-value = 2.32e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.48e-01

Only 100 of 227 total aligned reads displayed.

TGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCG  >  NC_014034/1397121‑1397321
                                                                                                     |                                                                                                     
 ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCAcc                                                                                                      <  1:3450517/101‑1 (MQ=255)
  gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCa                                                                                                     <  2:306185/101‑1 (MQ=255)
    tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGa                                                                                                   >  2:2545050/1‑101 (MQ=255)
    tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGa                                                                                                   >  1:3711049/1‑101 (MQ=255)
     cGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGAt                                                                                                  >  1:2836173/1‑101 (MQ=255)
     cGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGAt                                                                                                  <  2:2274331/101‑1 (MQ=255)
      gAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATc                                                                                                 >  1:3232409/1‑101 (MQ=255)
      gAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATc                                                                                                 >  2:1782988/1‑101 (MQ=255)
       aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATcc                                                                                                >  2:3037254/1‑101 (MQ=255)
       aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATcc                                                                                                <  1:3146884/101‑1 (MQ=255)
       aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATcc                                                                                                <  1:2165766/101‑1 (MQ=255)
       aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAGTCTGCCA.CGACGCCCTGCACCAGATcc                                                                                                >  2:2650/1‑101 (MQ=255)
        aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCcg                                                                                               >  1:1916182/1‑101 (MQ=255)
           gTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCgcgc                                                                                            >  2:4624959/1‑101 (MQ=255)
           gTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCgcgc                                                                                            >  2:243482/1‑101 (MQ=255)
               aGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTc                                                                                       <  2:3531063/101‑1 (MQ=255)
               aGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTc                                                                                       <  2:3911822/101‑1 (MQ=255)
                     cgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTccc                                                                                 >  1:1900238/1‑101 (MQ=255)
                          caTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcgc                                                                            <  1:2016102/101‑1 (MQ=255)
                          caTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcgc                                                                            <  1:3562592/101‑1 (MQ=255)
                           aTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcgcg                                                                           >  1:4573901/1‑101 (MQ=255)
                           aTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcgcg                                                                           >  2:1265797/1‑101 (MQ=255)
                            tGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCgg                                                                          <  1:1877147/101‑1 (MQ=255)
                            tGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCgg                                                                          <  2:2621619/101‑1 (MQ=255)
                             gTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCggg                                                                         >  1:3634401/1‑101 (MQ=255)
                               cTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGTCTGCCA.CGACGGCCTCCACCATATCCGCGGC.GCCGCTCCCCCCGCggggg                                                                       >  2:3717516/1‑101 (MQ=37)
                                ttCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGc                                                                      >  1:3052490/1‑101 (MQ=255)
                                 tCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCg                                                                     >  1:4693898/1‑101 (MQ=255)
                                  cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGt                                                                    >  1:2388142/1‑101 (MQ=255)
                                  cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCgg                                                                    >  1:4487708/1‑100 (MQ=255)
                                     ccGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                 >  2:602040/1‑101 (MQ=255)
                                      cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCTGCGGGGGCGTGGAt                                                                >  2:3138702/1‑101 (MQ=255)
                                      cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                >  1:429603/1‑101 (MQ=255)
                                       gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                               >  2:3598571/1‑101 (MQ=255)
                                       gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCCCGGGGGCGTGGGTc                                                               >  1:200696/1‑101 (MQ=255)
                                       gTGCGCCGGCGCCCTCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGTGGCGTGGATc                                                               <  1:4099684/101‑1 (MQ=38)
                                        tGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGTGGGCGTGGATCg                                                              <  1:2577142/101‑1 (MQ=255)
                                        ggcgcCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              <  1:3362447/100‑1 (MQ=255)
                                         gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCAACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              <  1:916041/101‑1 (MQ=2)
                                         gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCAACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              <  1:1591119/101‑1 (MQ=2)
                                         gcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                             <  1:1250738/101‑1 (MQ=255)
                                         cgatcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGC.TGGCGGCCCGGTCGTGAATCTCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              >  1:4062802/5‑101 (MQ=2)
                                          cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                            <  2:757506/101‑1 (MQ=255)
                                           gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTGTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCGCCCCCCGCGGGGGCGTGGATCGaaa                                                           <  1:3196984/101‑1 (MQ=255)
                                            gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCAACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                           <  1:2221811/100‑1 (MQ=2)
                                            ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                          <  1:1115594/101‑1 (MQ=255)
                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                        >  1:3324558/1‑101 (MQ=255)
                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                        >  1:1636018/1‑101 (MQ=255)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                                     >  2:636134/1‑101 (MQ=255)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                                     >  1:824799/1‑101 (MQ=255)
                                                   tCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCgcgc                                                   >  1:1350900/1‑101 (MQ=255)
                                                   tCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCgcgc                                                   >  1:3968426/1‑101 (MQ=255)
                                                    ccccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCa                                                  >  2:115182/1‑101 (MQ=255)
                                                     cccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAc                                                 <  2:1451917/101‑1 (MQ=255)
                                                         cgcgGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgcgc                                             <  2:4462399/101‑1 (MQ=255)
                                                         cgcgGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgcgc                                             >  2:487673/1‑101 (MQ=255)
                                                           cgGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGa                                           <  2:2715843/101‑1 (MQ=255)
                                                           cgGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGa                                           >  2:3706405/1‑101 (MQ=255)
                                                           cgGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGa                                           >  2:3392251/1‑101 (MQ=255)
                                                           cgGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGCATCGAAACAACCCGGCcgcgcgc                                           >  2:4235612/1‑100 (MQ=37)
                                                            gggggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGaa                                          <  2:4573648/101‑1 (MQ=255)
                                                             ggggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGaag                                         >  1:1819223/1‑100 (MQ=255)
                                                             ggggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCCCACGCCCGAAc                                         >  2:1755474/1‑101 (MQ=255)
                                                                 cGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCg                                     >  2:2052277/1‑101 (MQ=255)
                                                                 cGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCg                                     >  2:4170146/1‑101 (MQ=255)
                                                                    ggATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                                  >  1:2314321/1‑101 (MQ=255)
                                                                      aTCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGCAACACCGGGCACGCGCGAACGGCGTCAGc                                >  2:3891448/1‑101 (MQ=255)
                                                                       tCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcc                               <  1:2037716/101‑1 (MQ=255)
                                                                        cGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc                              >  1:2729149/1‑101 (MQ=255)
                                                                        cGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc                              >  1:598870/1‑101 (MQ=255)
                                                                         gAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                             <  2:1942844/101‑1 (MQ=255)
                                                                         gAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                             >  1:2441289/1‑101 (MQ=255)
                                                                         gAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                             <  1:297238/101‑1 (MQ=255)
                                                                          aaaCGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcg                            >  2:719221/1‑101 (MQ=255)
                                                                            aCGTGTCTGCCA.CGACGCCCTGCACTAGATACGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACACCGCGCACGCGCGCACCGCCTCAGCCCcgcg                          >  2:3257089/1‑101 (MQ=37)
                                                                              gtgtCTGCCC.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa                        <  1:2640068/101‑1 (MQ=255)
                                                                              gtgtCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa                        >  1:1002314/1‑101 (MQ=255)
                                                                               tgtCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGCAc                       >  2:1055669/1‑101 (MQ=255)
                                                                               tgtCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc                       <  2:2115297/101‑1 (MQ=255)
                                                                               tgtCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc                       <  2:4314565/101‑1 (MQ=255)
                                                                                gtCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACg                      >  2:1876408/1‑101 (MQ=255)
                                                                                 tCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGt                     >  2:1606953/1‑101 (MQ=255)
                                                                                 tCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGt                     <  1:636198/101‑1 (MQ=255)
                                                                                  cTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTc                    <  2:1635918/101‑1 (MQ=255)
                                                                                   tGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCg                   >  1:1557235/1‑101 (MQ=255)
                                                                                   tGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCg                   >  1:4537357/1‑101 (MQ=255)
                                                                                   tGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCg                   >  2:4369188/1‑101 (MQ=255)
                                                                                     ccA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCt                 >  2:905689/1‑101 (MQ=255)
                                                                                        ccgacgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcg               <  2:1986719/100‑2 (MQ=255)
                                                                                          gacgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccc             >  2:3355915/1‑101 (MQ=255)
                                                                                           acgCCCTGCCCCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTccccc            <  2:120558/101‑1 (MQ=255)
                                                                                            cgCCCTGCCCCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccccc           <  1:1707887/101‑1 (MQ=255)
                                                                                             gCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcg          <  1:4369435/101‑1 (MQ=255)
                                                                                                 tGCACCAGATCCGCGCT.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCggg      >  1:723438/1‑101 (MQ=255)
                                                                                                 tGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCggg      <  2:4073580/101‑1 (MQ=255)
                                                                                                 tGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCggg      <  2:824726/101‑1 (MQ=255)
                                                                                                 cgCACCAGCTCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCggg      <  2:4525893/100‑1 (MQ=255)
                                                                                                    aCCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGc   >  1:2517041/1‑101 (MQ=255)
                                                                                                     ccAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAGGTCGCTCCCCCCGCGGGAGCg  >  1:3868310/1‑101 (MQ=255)
                                                                                                     ccAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCg  <  2:3050192/101‑1 (MQ=255)
                                                                                                     |                                                                                                     
TGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCG  >  NC_014034/1397121‑1397321

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG