Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,229 G→A 4.1% A371T (GCG→ACA)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2290G→A 4.1% 11.5 ‑3.8 ‑0.1196A371T (GCG→ACG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/7):  ref base (108/79):  total (110/86)
Fisher's exact test strand distribution p-value = 2.27e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.78e-01

Only 100 of 210 total aligned reads displayed.

AACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCG  >  NC_014034/1397129‑1397328
                                                                                                     |                                                                                                    
aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCcg                                                                                                      <  2:1973916/101‑1 (MQ=255)
    tCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGcc                                                                                                  <  1:1718540/101‑1 (MQ=255)
     ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.g                                                                                                <  2:61105/101‑1 (MQ=255)
       aGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTc                                                                                              <  2:3531063/101‑1 (MQ=255)
        gCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCg                                                                                             >  1:4313532/1‑101 (MQ=255)
           cgcgCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTc                                                                                          <  1:832878/101‑1 (MQ=255)
              gcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTcccc                                                                                       <  2:3048076/101‑1 (MQ=255)
                cacaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTcccccc                                                                                     <  2:408907/101‑1 (MQ=255)
                  caTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcgc                                                                                   <  1:3562592/101‑1 (MQ=255)
                  caTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcgc                                                                                   <  1:2016102/101‑1 (MQ=255)
                   aTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcgcg                                                                                  >  1:4573901/1‑101 (MQ=255)
                   aTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcgcg                                                                                  >  2:1265797/1‑101 (MQ=255)
                    tGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCgg                                                                                 <  1:1877147/101‑1 (MQ=255)
                    tGTCTTCGACCGTGCGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCgg                                                                                 <  1:1636604/101‑1 (MQ=255)
                        ttCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGc                                                                             >  1:3052490/1‑101 (MQ=255)
                        ttCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGc                                                                             >  2:2639914/1‑101 (MQ=255)
                         tCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCg                                                                            <  1:3698931/101‑1 (MQ=255)
                         tCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCg                                                                            >  1:4693898/1‑101 (MQ=255)
                          cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCgg                                                                           >  2:75198/1‑100 (MQ=255)
                          cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGt                                                                           >  1:2388142/1‑101 (MQ=255)
                              cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCTGCGGGGGCGTGGAt                                                                       >  2:3138702/1‑101 (MQ=255)
                              cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       <  1:1444410/101‑1 (MQ=255)
                               gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      >  2:3598571/1‑101 (MQ=255)
                               gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCCCGGGGGCGTGGGTc                                                                      >  1:200696/1‑101 (MQ=255)
                               gTGCGCCGGCGCCCTCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGTGGCGTGGATc                                                                      <  1:4099684/101‑1 (MQ=38)
                                 cgatcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGC.TGGCGGCCCGGTCGTGAATCTCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                     >  1:4062802/5‑101 (MQ=2)
                                  tatcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCCGCGGC.GCCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                    >  1:3204046/4‑101 (MQ=2)
                                    gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCAACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  <  2:2727066/100‑1 (MQ=2)
                                    gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCAACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  <  2:337615/100‑1 (MQ=2)
                                    gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCAACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  <  1:2221811/100‑1 (MQ=2)
                                    ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                 <  1:1115594/101‑1 (MQ=255)
                                     cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                                <  2:3914230/101‑1 (MQ=255)
                                     cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                                >  2:305714/1‑101 (MQ=255)
                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                               >  1:314792/1‑101 (MQ=255)
                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                               <  2:3728638/101‑1 (MQ=255)
                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                               >  2:4216297/1‑101 (MQ=255)
                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAcc                                                              >  1:2908308/1‑101 (MQ=255)
                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                                            <  2:793657/101‑1 (MQ=255)
                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                                            >  2:4382313/1‑101 (MQ=255)
                                          cTCCCCCCGCGGGGGCGTGGATCGAAACGCGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgcg                                                           >  1:3341568/1‑101 (MQ=255)
                                            ccccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCa                                                         >  2:115182/1‑101 (MQ=255)
                                             cccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGTCGTGGATCGAAACACCGCGCAc                                                        <  2:688099/101‑1 (MQ=255)
                                             cccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAc                                                        <  2:1451917/101‑1 (MQ=255)
                                               cccGGGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgc                                                      <  2:586130/101‑1 (MQ=255)
                                                   ctggggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGa                                                  <  2:1819114/99‑1 (MQ=255)
                                                   cgGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGa                                                  <  2:2715843/101‑1 (MQ=255)
                                                   cgGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGa                                                  >  2:3706405/1‑101 (MQ=255)
                                                    gggggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGaa                                                 >  1:1942961/1‑101 (MQ=255)
                                                     ggggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGaag                                                >  1:1819223/1‑100 (MQ=255)
                                                     ggggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCCCACGCCCGAAc                                                >  2:1755474/1‑101 (MQ=255)
                                                         cGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCg                                            >  2:4170146/1‑101 (MQ=255)
                                                         cGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCg                                            >  2:2052277/1‑101 (MQ=255)
                                                         cGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCg                                            >  2:458698/1‑101 (MQ=255)
                                                            ggATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                                         >  1:2314321/1‑101 (MQ=255)
                                                            ggATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                                         >  1:771139/1‑101 (MQ=255)
                                                            ggATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                                         >  1:1915049/1‑101 (MQ=255)
                                                            ggATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGAGCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                                         <  2:2576199/101‑1 (MQ=255)
                                                             gATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAg                                        >  2:470730/1‑101 (MQ=255)
                                                              aTCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGc                                       >  1:1330050/1‑101 (MQ=255)
                                                              aTCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGc                                       >  2:3293997/1‑101 (MQ=255)
                                                               tCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcc                                      <  1:2037716/101‑1 (MQ=255)
                                                               tCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcc                                      <  2:1506648/101‑1 (MQ=255)
                                                                cGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc                                     >  1:598870/1‑101 (MQ=255)
                                                                 gAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                                    <  1:917770/101‑1 (MQ=255)
                                                                 gAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                                    >  1:2441289/1‑101 (MQ=255)
                                                                 gAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                                    <  2:4313285/101‑1 (MQ=255)
                                                                 gAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                                    >  1:2989686/1‑101 (MQ=255)
                                                                  aaaCGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcg                                   >  2:3430653/1‑101 (MQ=255)
                                                                    aCGTGTCTGCCA.CGCCGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcgcg                                 <  1:1577406/101‑1 (MQ=255)
                                                                      gtgtCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa                               >  1:1002314/1‑101 (MQ=255)
                                                                      gtgtCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa                               >  1:1595819/1‑101 (MQ=255)
                                                                      gtgtCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa                               >  1:2584528/1‑101 (MQ=255)
                                                                      gtgtCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa                               >  1:3590343/1‑101 (MQ=255)
                                                                       tgtCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc                              <  2:2115297/101‑1 (MQ=255)
                                                                        gtCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACg                             >  2:1876408/1‑101 (MQ=255)
                                                                         tCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGt                            >  2:1606953/1‑101 (MQ=255)
                                                                         tCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGt                            <  1:636198/101‑1 (MQ=255)
                                                                          cTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTc                           <  2:1635918/101‑1 (MQ=255)
                                                                           taacA.CGAAGCCCTGCACCAGATCCGTGCC.GTCGCCTCCGCCGCGGAGGCGCTGCTCCCACGACCGCGCACGCGCGTAGCGCGGCAGTCCCCCGAACGCCg                          >  2:3901043/4‑101 (MQ=255)
                                                                           tGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCg                          >  2:3247741/1‑101 (MQ=255)
                                                                           tGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCg                          >  1:4537357/1‑101 (MQ=255)
                                                                           tGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCg                          >  2:4369188/1‑101 (MQ=255)
                                                                            gCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGc                         <  1:3975146/101‑1 (MQ=255)
                                                                             ccA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCt                        >  2:905689/1‑101 (MQ=255)
                                                                             ccA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCCCt                        <  1:2715082/101‑1 (MQ=255)
                                                                              cA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTc                       <  2:2484602/101‑1 (MQ=255)
                                                                                   acgCCCTGCCCCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTccccc                   <  2:120558/101‑1 (MQ=255)
                                                                                     gCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcg                 <  1:4369435/101‑1 (MQ=255)
                                                                                        cTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCgg              >  1:3309977/1‑101 (MQ=255)
                                                                                         tGCACCAGATCCGCGCT.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCggg             >  1:723438/1‑101 (MQ=255)
                                                                                         tGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCggg             <  2:2014887/101‑1 (MQ=255)
                                                                                         tGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGACCGAAACACCGCGCACGCGCGCATGGCGTAAGCCCCGCGACCGTCGCTTCCCCCGCggg             >  2:2275070/1‑101 (MQ=37)
                                                                                         cgCACCAGCTCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCggg             <  2:4525893/100‑1 (MQ=255)
                                                                                            aCCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGc          >  1:2517041/1‑101 (MQ=255)
                                                                                             ccAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAGGTCGCTCCCCCCGCGGGAGCg         >  1:3868310/1‑101 (MQ=255)
                                                                                               aGATCCGCGCC.GTCGCTCCCCCCGCGGGGGGGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTg       >  1:4222772/1‑101 (MQ=255)
                                                                                               aGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTg       >  2:4628669/1‑101 (MQ=255)
                                                                                                gATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTgg      >  1:4522397/1‑101 (MQ=255)
                                                                                                gATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGCgg      >  2:3835662/1‑101 (MQ=255)
                                                                                                   ccGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATc   <  1:3430860/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
AACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCG  >  NC_014034/1397129‑1397328

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG