Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,233 C→T 4.5% P372L (CCG→CTG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2330C→T 4.5% 14.5 ‑2.5 ‑0.1200P372L (CCG→CTG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/7):  ref base (109/82):  total (111/89)
Fisher's exact test strand distribution p-value = 8.07e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.58e-01

Only 100 of 218 total aligned reads displayed.

TCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACG  >  NC_014034/1397133‑1397333
                                                                                                     |                                                                                                         
tCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGcc                                                                                                           <  1:1718540/101‑1 (MQ=255)
   aGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTc                                                                                                       >  2:3489031/1‑101 (MQ=255)
    gCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCg                                                                                                      >  1:4313532/1‑101 (MQ=255)
       cgcgCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTc                                                                                                   <  1:832878/101‑1 (MQ=255)
         cgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTccc                                                                                                 <  1:3240935/101‑1 (MQ=255)
          gcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTcccc                                                                                                <  2:3048076/101‑1 (MQ=255)
           ccACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTccccc                                                                                               <  2:2529536/101‑1 (MQ=255)
              caTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcgc                                                                                            <  1:2016102/101‑1 (MQ=255)
               aTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcgcg                                                                                           >  1:4573901/1‑101 (MQ=255)
               aTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcgcg                                                                                           >  2:1265797/1‑101 (MQ=255)
                tGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCgg                                                                                          <  2:2621619/101‑1 (MQ=255)
                tGTCTTCGACCGTGCGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCgg                                                                                          <  1:1636604/101‑1 (MQ=255)
                 gTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCggg                                                                                         >  1:3634401/1‑101 (MQ=255)
                       gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAAGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTg                                                                                   >  2:1876260/1‑101 (MQ=255)
                        gccGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                                  <  1:3037442/100‑1 (MQ=255)
                         ccGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                                 >  2:602040/1‑101 (MQ=255)
                          cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCTGCGGGGGCGTGGAt                                                                                >  2:3138702/1‑101 (MQ=255)
                          cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                                <  1:3526513/101‑1 (MQ=255)
                           gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCCCGGGGGCGTGGGTc                                                                               >  1:200696/1‑101 (MQ=255)
                           gTGCGCCGGCGCCCTCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGTGGCGTGGATc                                                                               <  1:4099684/101‑1 (MQ=38)
                            tGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                              >  1:186109/1‑101 (MQ=255)
                             gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCAACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                              <  1:1591119/101‑1 (MQ=2)
                             gcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                             <  1:1250738/101‑1 (MQ=255)
                             cgatcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGC.TGGCGGCCCGGTCGTGAATCTCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                              >  1:4062802/5‑101 (MQ=2)
                              cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCACCAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                             >  1:1880939/4‑101 (MQ=2)
                              cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                            <  2:757506/101‑1 (MQ=255)
                               gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTGTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCGCCCCCCGCGGGGGCGTGGATCGaaa                                                                           <  1:3196984/101‑1 (MQ=255)
                                gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCAACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                           <  1:2221811/100‑1 (MQ=2)
                                gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCAACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                           <  2:2727066/100‑1 (MQ=2)
                                ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                          <  1:1115594/101‑1 (MQ=255)
                                 cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                                         <  2:4474970/101‑1 (MQ=255)
                                 cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                                         >  2:305714/1‑101 (MQ=255)
                                 cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                                         <  2:3914230/101‑1 (MQ=255)
                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                                        <  2:3728638/101‑1 (MQ=255)
                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                                        <  2:2242322/101‑1 (MQ=255)
                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                                        >  1:3324558/1‑101 (MQ=255)
                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAcc                                                                       >  1:2908308/1‑101 (MQ=255)
                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                                                     <  2:793657/101‑1 (MQ=255)
                                      cTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc.g                                                                   >  2:3715565/1‑101 (MQ=255)
                                       tCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCgc.gc                                                                  >  1:1350900/1‑101 (MQ=255)
                                       tCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCgc.gc                                                                  >  1:3968426/1‑101 (MQ=255)
                                        ccccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCa                                                                 >  2:115182/1‑101 (MQ=255)
                                        ccccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCa                                                                 >  2:3533596/1‑101 (MQ=255)
                                        ccccccGCGGGGGCGTGGATCCAAACGTTTCTGCCA.CGACGCCCTGCACCAGCTCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCa                                                                 <  1:3577386/101‑1 (MQ=255)
                                         cccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCAc                                                                <  2:1451917/101‑1 (MQ=255)
                                             cgcgGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCAcgcgc                                                            >  2:487673/1‑101 (MQ=255)
                                               cgGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGa                                                          <  2:2715843/101‑1 (MQ=255)
                                               cgGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGa                                                          >  2:3392251/1‑101 (MQ=255)
                                               cgGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGCATCGAAACAACCC.GGCcgcgcgc                                                          >  2:4235612/1‑100 (MQ=37)
                                                gggggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGaa                                                         <  2:4573648/101‑1 (MQ=255)
                                                gggggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGaa                                                         >  2:1733771/1‑101 (MQ=255)
                                                      gTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.Gt                                                  >  1:2509317/1‑101 (MQ=255)
                                                        ggATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.a                                               >  1:771139/1‑101 (MQ=255)
                                                        ggATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.a                                               >  1:2314321/1‑101 (MQ=255)
                                                        ggATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.a                                               >  1:1915049/1‑101 (MQ=255)
                                                           tCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.c                                           <  2:1506648/101‑1 (MQ=255)
                                                           tCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.c                                           <  1:2037716/101‑1 (MQ=255)
                                                             gAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                         <  1:917770/101‑1 (MQ=255)
                                                             gAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                         <  1:602104/101‑1 (MQ=255)
                                                             gAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                         >  2:2020094/1‑101 (MQ=255)
                                                              aaaCGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcg                                        >  2:719221/1‑101 (MQ=255)
                                                              aaaCGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GCC.AGc.cccc                                        >  1:2404391/1‑100 (MQ=255)
                                                                aCGTGTCTGCCA.CGACGCCCTGCACTAGATACGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACACCGC.GCACGCGCGCACCGC.CTC.AGC.CCcgcg                                      >  2:3257089/1‑101 (MQ=37)
                                                                  gtgtCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGaa                                    >  1:1002314/1‑101 (MQ=255)
                                                                  gtgtCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGaa                                    >  1:2584528/1‑101 (MQ=255)
                                                                   tgtCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAAc                                   <  2:4314565/101‑1 (MQ=255)
                                                                   tgtCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAAc                                   >  1:3748118/1‑101 (MQ=255)
                                                                    gtCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACg                                  <  1:2545195/101‑1 (MQ=255)
                                                                     tCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGt                                 >  2:1606953/1‑101 (MQ=255)
                                                                      cTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTc                                >  2:1767154/1‑101 (MQ=255)
                                                                       taacA.CGAAGCCCTGCACCAGATCCGTGCC.GTCGCCTCCGCCGCGGAGGCGCTGCTCCCACGACCGC.GCACGCGCGTAGCGC.GGC.AGT.CCCCCGAACGCCg                               >  2:3901043/4‑101 (MQ=255)
                                                                       tGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCg                               >  1:1557235/1‑101 (MQ=255)
                                                                       tGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCg                               >  1:4537357/1‑101 (MQ=255)
                                                                         ccA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCt                             >  2:4212284/1‑101 (MQ=255)
                                                                         ccA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCt                             >  2:905689/1‑101 (MQ=255)
                                                                         ccA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCCCt                             <  1:2715082/101‑1 (MQ=255)
                                                                            ccgacgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTcg                           <  2:1986719/100‑2 (MQ=255)
                                                                              gacgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTcccc                         >  2:3355915/1‑101 (MQ=255)
                                                                              gacgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTcccc                         <  2:4381541/101‑1 (MQ=255)
                                                                               acgCCCTGCCCCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTccccc                        <  2:120558/101‑1 (MQ=255)
                                                                                cgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTcccccc                       <  2:1916070/101‑1 (MQ=255)
                                                                                cgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTcccccc                       <  1:305639/101‑1 (MQ=255)
                                                                                 gCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCcg                      <  1:4369435/101‑1 (MQ=255)
                                                                                     tGCACCAGATCCGCGCT.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCggg                  >  1:723438/1‑101 (MQ=255)
                                                                                           ggccctcgcac.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGAC.GGA.CCCGC..CCGTCGCTCCCCCCGCGGGGGCGTg            <  2:2763590/91‑1 (MQ=2)
                                                                                           aGATCCGCGCC.GTCGCTCCCCCCGCGGGGGGGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTg            >  1:4222772/1‑101 (MQ=255)
                                                                                           aGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTg            >  2:4628669/1‑101 (MQ=255)
                                                                                            gATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTgg           >  1:503703/1‑101 (MQ=255)
                                                                                            gATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTgg           >  1:4522397/1‑101 (MQ=255)
                                                                                            gATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTgg           >  2:4144851/1‑101 (MQ=255)
                                                                                            gATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGCgg           >  2:3835662/1‑101 (MQ=255)
                                                                                               ccGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATc        <  1:3430860/101‑1 (MQ=255)
                                                                                                  cgcC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaa     <  2:641752/101‑1 (MQ=255)
                                                                                                   gcC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGCACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaaa    <  1:4663260/101‑1 (MQ=255)
                                                                                                   gcC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaaa    <  2:2083607/101‑1 (MQ=255)
                                                                                                    cc.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCCGT.GCGCGCCATGAATTC.ATCGAGCGCCATCGAGCGGCGCTCCCCCCGCGGGGGCGTGGCTCGaa     >  2:3690009/1‑101 (MQ=2)
                                                                                                    cc.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAAc   <  2:2314188/101‑1 (MQ=255)
                                                                                                     c.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCCCGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaaaag  >  2:1084881/1‑99 (MQ=255)
                                                                                                     c.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg  >  2:467856/1‑101 (MQ=255)
                                                                                                     c.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg  >  1:180432/1‑101 (MQ=255)
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TCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACG  >  NC_014034/1397133‑1397333

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG