Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,276 C→G 3.0% R386R (CGC→CGG RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2760C→G 3.0% 6.0 ‑2.2 ‑0.5199R386R (CGC→CGGRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/5):  ref base (100/92):  total (101/98)
Fisher's exact test strand distribution p-value = 1.13e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.86e-01

Only 100 of 216 total aligned reads displayed.

CCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCC  >  NC_014034/1397176‑1397376
                                                                                                     |                                                                                                       
cccGGGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCAcgc                                                                                                         <  2:586130/101‑1 (MQ=255)
    cgGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGa                                                                                                     >  2:3706405/1‑101 (MQ=255)
    cgGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGa                                                                                                     <  2:2715843/101‑1 (MQ=255)
    cgGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGa                                                                                                     >  2:3392251/1‑101 (MQ=255)
     gggggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGaa                                                                                                    <  2:4573648/101‑1 (MQ=255)
     gggggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGaa                                                                                                    >  2:1733771/1‑101 (MQ=255)
      ggggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGaag                                                                                                   >  1:1819223/1‑100 (MQ=255)
      ggggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAAc                                                                                                   >  2:3137701/1‑101 (MQ=255)
       gggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACg                                                                                                  >  2:3753936/1‑101 (MQ=255)
       gggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACg                                                                                                  >  2:1097920/1‑101 (MQ=255)
        ggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACgg                                                                                                 <  2:1333082/101‑1 (MQ=255)
           gTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.Gt                                                                                             >  1:2509317/1‑101 (MQ=255)
             ggATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCa                                                                                           >  1:771139/1‑101 (MQ=255)
             ggATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCa                                                                                           >  1:2314321/1‑101 (MQ=255)
             ggATCGAAACGTGCCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCa                                                                                           >  2:3689611/1‑101 (MQ=255)
                tCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGcc                                                                                        <  1:2037716/101‑1 (MQ=255)
                tCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGcc                                                                                        <  2:1506648/101‑1 (MQ=255)
                 cGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGccc                                                                                       >  1:598870/1‑101 (MQ=255)
                 cGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGccc                                                                                       >  1:2729149/1‑101 (MQ=255)
                  gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGcccc                                                                                      <  2:4313285/101‑1 (MQ=255)
                  gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGcccc                                                                                      >  2:2020094/1‑101 (MQ=255)
                  gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGcccc                                                                                      <  1:917770/101‑1 (MQ=255)
                  gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGcccc                                                                                      <  1:297238/101‑1 (MQ=255)
                   aaaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GCCAGccccc                                                                                     >  1:2404391/1‑100 (MQ=255)
                     aCGTGTCTGCCACGACGCCCTGCACTAGATACGCGCCGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACACCGC.GCACGCGCGCACCGC.CTCAGCCCcgcg                                                                                   >  2:3257089/1‑101 (MQ=37)
                       gtgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGaa                                                                                 >  1:2584528/1‑101 (MQ=255)
                       gtgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGaa                                                                                 >  1:1002314/1‑101 (MQ=255)
                        tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGCA..c                                                                              >  2:1055669/1‑101 (MQ=255)
                        tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..c                                                                              >  1:4527640/1‑101 (MQ=255)
                          tCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGt                                                                            >  2:1606953/1‑101 (MQ=255)
                          tCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGt                                                                            <  1:636198/101‑1 (MQ=255)
                           cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTc                                                                           >  2:1767154/1‑101 (MQ=255)
                            taacACGAAGCCCTGCACCAGATCCGTGCCGTCGCCTCCGCCGCGGAGGCGCTGCTCCCACGACCGC.GCACGCGCGTAGCGC.GGCAGTCCCCCGAA..CGCCg                                                                          >  2:3901043/4‑101 (MQ=255)
                            tGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCg                                                                          >  2:3247741/1‑101 (MQ=255)
                            tGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCg                                                                          >  2:4369188/1‑101 (MQ=255)
                             gCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGc                                                                         <  1:3975146/101‑1 (MQ=255)
                             gCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGc                                                                         >  2:2075211/1‑101 (MQ=255)
                              ccACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCt                                                                        >  2:4212284/1‑101 (MQ=255)
                               cACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTc                                                                       <  2:2484602/101‑1 (MQ=255)
                                    cgCCCTGCCCCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTcccccc                                                                  <  1:1707887/101‑1 (MQ=255)
                                    cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTcccccc                                                                  <  2:1916070/101‑1 (MQ=255)
                                         tGCACCAGATCCGCGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCggg                                                             >  1:723438/1‑101 (MQ=255)
                                         tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCggg                                                             <  1:217218/101‑1 (MQ=255)
                                         tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCggg                                                             <  2:824726/101‑1 (MQ=255)
                                         cgCACCAGCTCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCggg                                                             <  2:4525893/100‑1 (MQ=255)
                                               ggccctcgcacGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGACGGACCCGC..C..CGTCGCTCCCCCCGCGGGGGCGTg                                                       <  2:2763590/91‑1 (MQ=2)
                                               aGATCCGCGCCGTCGCTCCCCCCGCGGGGGGGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTg                                                       >  1:4222772/1‑101 (MQ=255)
                                               aGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTg                                                       >  2:4628669/1‑101 (MQ=255)
                                                gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTgg                                                      >  2:4144851/1‑101 (MQ=255)
                                                   ccGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATc                                                   <  1:3430860/101‑1 (MQ=255)
                                                    cgcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCg                                                  <  2:2908132/101‑1 (MQ=255)
                                                       gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGCACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGaaa                                               <  1:4663260/101‑1 (MQ=255)
                                                        ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAAc                                              <  2:2314188/101‑1 (MQ=255)
                                                          gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACgg                                            <  1:1182131/101‑1 (MQ=255)
                                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                         >  1:3649724/1‑101 (MQ=255)
                                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                         >  1:1936383/1‑101 (MQ=255)
                                                              gatcgcccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                        >  2:1974551/6‑101 (MQ=255)
                                                              ccccccccCGGGGGGCGTGGATCGAAACACCCC.GCACCTGTCGGCGGC.G..AACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatcgat                                        <  2:1989096/99‑7 (MQ=0)
                                                              cTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                        >  1:1058578/1‑101 (MQ=255)
                                                                ccccccGCGGGGGCGTGGATCGAAACCCCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTAc                                      <  1:2655/101‑1 (MQ=255)
                                                                     cgcgGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGACGGACCCGC..C..CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgccc                                 >  2:4457308/1‑91 (MQ=0)
                                                                      gcgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                <  2:2170819/101‑1 (MQ=255)
                                                                       cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                               >  2:3379979/1‑101 (MQ=255)
                                                                       cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                               <  2:2642265/101‑1 (MQ=255)
                                                                       cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                               <  1:3533815/101‑1 (MQ=255)
                                                                       cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                               >  1:3868288/1‑101 (MQ=255)
                                                                        gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                              <  1:3356117/101‑1 (MQ=255)
                                                                        gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                              >  2:2756886/1‑101 (MQ=255)
                                                                           ggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCgcgc                           >  1:3944103/1‑101 (MQ=255)
                                                                            gCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcc                          >  1:1584606/1‑101 (MQ=255)
                                                                            gCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcc                          <  2:4008288/101‑1 (MQ=255)
                                                                             cGTGGACCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccc                         <  2:3324356/101‑1 (MQ=255)
                                                                              gTGGATCGAAACCCCGC.GCCCGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                        <  1:3117436/101‑1 (MQ=255)
                                                                              gTGGATCGAAACCCCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                        <  1:2800999/101‑1 (MQ=255)
                                                                               tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccccc                       >  2:1222747/1‑100 (MQ=255)
                                                                               tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                       >  1:1254118/1‑101 (MQ=255)
                                                                                ggATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                      >  1:154644/1‑101 (MQ=255)
                                                                                ggATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                      <  1:1783097/101‑1 (MQ=255)
                                                                                ggATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                      <  1:885534/101‑1 (MQ=255)
                                                                                ggATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                      >  1:2014616/1‑101 (MQ=255)
                                                                                ggATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                      >  1:4174102/1‑101 (MQ=255)
                                                                                ggATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                      <  2:665116/101‑1 (MQ=255)
                                                                                    cGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCa                  <  2:4693642/101‑1 (MQ=255)
                                                                                    cGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCa                  <  2:186084/101‑1 (MQ=255)
                                                                                    cGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCa                  <  1:4382560/101‑1 (MQ=255)
                                                                                     gAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAg                 <  1:1338806/101‑1 (MQ=255)
                                                                                     gAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAg                 <  1:4332479/101‑1 (MQ=255)
                                                                                     gAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAg                 >  2:853518/1‑101 (MQ=255)
                                                                                         cacCGC.GCCCGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg             <  1:3790313/101‑1 (MQ=255)
                                                                                         cacCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg             <  2:730108/101‑1 (MQ=255)
                                                                                         cacCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg             >  2:3499953/1‑101 (MQ=255)
                                                                                         cacCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg             >  2:840500/1‑101 (MQ=255)
                                                                                          acCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgga            >  2:464400/1‑101 (MQ=255)
                                                                                          acCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgga            <  1:2052398/101‑1 (MQ=255)
                                                                                             gc.gcACGCGCGAACGGC.GTCCGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCCCGCCCCACGCCAGACGGACGt         >  2:2254652/1‑101 (MQ=255)
                                                                                             gc.gcACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGt         <  1:2561423/101‑1 (MQ=255)
                                                                                              c.gcACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTc        <  1:3294199/101‑1 (MQ=255)
                                                                                                gcACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCg       <  1:3248366/101‑1 (MQ=255)
                                                                                                gcACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCg       <  2:200671/101‑1 (MQ=255)
                                                                                                     cgcgAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccc  >  1:1425434/1‑101 (MQ=255)
                                                                                                     |                                                                                                       
CCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCC  >  NC_014034/1397176‑1397376

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG