Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,280 A→T 3.1% N388Y (AAC→TAT)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2800A→T 3.1% 9.2 ‑1.6 ‑0.3192N388Y (AAC→TAC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/5):  ref base (93/90):  total (95/97)
Fisher's exact test strand distribution p-value = 2.11e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.95e-01

Only 100 of 214 total aligned reads displayed.

CGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCC  >  NC_014034/1397180‑1397379
                                                                                                     |                                                                                                      
ctggggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGa                                                                                                        <  2:1819114/99‑1 (MQ=255)
 gggggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGaa                                                                                                       <  2:4573648/101‑1 (MQ=255)
  ggggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGaag                                                                                                      >  1:1819223/1‑100 (MQ=255)
  ggggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.CCACGCCCGAAc                                                                                                      >  2:1755474/1‑101 (MQ=255)
   gggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACg                                                                                                     >  2:1097920/1‑101 (MQ=255)
    ggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACgg                                                                                                    <  2:3232208/101‑1 (MQ=255)
      cGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.g                                                                                                 >  2:4170146/1‑101 (MQ=255)
      cGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.g                                                                                                 >  2:2052277/1‑101 (MQ=255)
       gTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.Gt                                                                                                >  1:2509317/1‑101 (MQ=255)
        tGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTc                                                                                               >  2:1548726/1‑101 (MQ=255)
         ggATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.a                                                                                             >  1:2314321/1‑101 (MQ=255)
         ggATCGAAACGTGTCTGCCACGACGCCCTGCACCAGAGCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.a                                                                                             <  2:2576199/101‑1 (MQ=255)
          gATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.Ag                                                                                            >  2:470730/1‑101 (MQ=255)
             cGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.cc                                                                                        >  1:598870/1‑101 (MQ=255)
             cGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.cc                                                                                        >  1:2729149/1‑101 (MQ=255)
              gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                                                                       <  2:1942844/101‑1 (MQ=255)
              gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                                                                       <  2:4313285/101‑1 (MQ=255)
              gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                                                                       <  1:297238/101‑1 (MQ=255)
               aaaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcg                                                                                      >  2:3430653/1‑101 (MQ=255)
               aaaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GCC.AGc.cccc                                                                                      >  1:2404391/1‑100 (MQ=255)
                 aCGTGTCTGCCACGCCGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcgcg                                                                                    <  1:1577406/101‑1 (MQ=255)
                   gtgtCTGCCCCGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGaa                                                                                  <  1:2640068/101‑1 (MQ=255)
                   gtgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGaa                                                                                  >  1:1002314/1‑101 (MQ=255)
                    tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAAc                                                                                 >  1:4527640/1‑101 (MQ=255)
                    tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAAc                                                                                 >  1:3748118/1‑101 (MQ=255)
                     gtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACg                                                                                <  1:2545195/101‑1 (MQ=255)
                      tCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGt                                                                               <  1:636198/101‑1 (MQ=255)
                       cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTc                                                                              <  2:1635918/101‑1 (MQ=255)
                        taacACGAAGCCCTGCACCAGATCCGTGCCGTCGCCTCCGCCGCGGAGGCGCTGCTCCCACGACCGC.GCACGCGCGTAGCGC.GGC.AGT.CCCCCGAACGCCg                                                                             >  2:3901043/4‑101 (MQ=255)
                        tGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCg                                                                             >  2:4369188/1‑101 (MQ=255)
                           cACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTc                                                                          <  2:2696712/101‑1 (MQ=255)
                            ccgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTcg                                                                         <  2:1986719/100‑2 (MQ=255)
                              gacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTcccc                                                                       >  2:1236384/1‑101 (MQ=255)
                               acgCCCTGCCCCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTccccc                                                                      <  2:120558/101‑1 (MQ=255)
                                cgCCCTGCCCCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTcccccc                                                                     <  1:1707887/101‑1 (MQ=255)
                                cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTcccccc                                                                     <  1:2020211/101‑1 (MQ=255)
                                cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTcccccc                                                                     <  1:305639/101‑1 (MQ=255)
                                    cTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCgg                                                                 >  1:3309977/1‑101 (MQ=255)
                                     tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCggg                                                                <  2:824726/101‑1 (MQ=255)
                                     tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCggg                                                                <  1:217218/101‑1 (MQ=255)
                                     tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCggg                                                                <  2:2014887/101‑1 (MQ=255)
                                     tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGACCGAAACACCGC.GCACGCGCGCATGGC.GTA.AGC.CCCGCGACCGTCGCTTCCCCCGCggg                                                                >  2:2275070/1‑101 (MQ=37)
                                     cgCACCAGCTCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCggg                                                                <  2:4525893/100‑1 (MQ=255)
                                         ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAAGGTCGCTCCCCCCGCGGGAGCg                                                            >  1:3868310/1‑101 (MQ=255)
                                         ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCg                                                            <  2:3050192/101‑1 (MQ=255)
                                           aGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTg                                                          >  2:4628669/1‑101 (MQ=255)
                                            gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGCgg                                                         >  2:3835662/1‑101 (MQ=255)
                                              tCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGAt                                                       <  2:1458323/101‑1 (MQ=255)
                                               ccGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATc                                                      <  1:3430860/101‑1 (MQ=255)
                                                   gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaaa                                                  <  2:2083607/101‑1 (MQ=255)
                                                    ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCCGT.GCGCGCCATGAATTC.ATCGAGCGCCATCGAGCGGCGCTCCCCCCGCGGGGGCGTGGCTCGaa                                                   >  2:3690009/1‑101 (MQ=2)
                                                     cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                >  2:467856/1‑101 (MQ=255)
                                                      gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACgg                                               <  1:1182131/101‑1 (MQ=255)
                                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGc                                              >  2:442795/1‑101 (MQ=255)
                                                        cGCTCCCCCCGCAGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCt                                             >  1:2569660/1‑101 (MQ=255)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                            >  1:1936383/1‑101 (MQ=255)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                            >  1:3649724/1‑101 (MQ=255)
                                                          cTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                           >  1:1058578/1‑101 (MQ=255)
                                                           cccccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTa                                          <  1:4216544/100‑1 (MQ=255)
                                                             cccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACa                                        <  1:3239325/101‑1 (MQ=255)
                                                              ccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAg                                       >  2:3248165/1‑101 (MQ=255)
                                                                ccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGAC.GGA.CCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgcc                                     <  2:1993593/101‑10 (MQ=0)
                                                                 cgcgGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGAC.GGA.CCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgcca                                    <  2:2416538/101‑11 (MQ=0)
                                                                  gggggggCGTGGATCGAAACCCCGC.GCACGCGCGAACGGC.GTC.CGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                   <  1:3285559/99‑1 (MQ=255)
                                                                   cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                  <  1:3533815/101‑1 (MQ=255)
                                                                   cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                  >  2:3379979/1‑101 (MQ=255)
                                                                    gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                 >  2:2207947/1‑101 (MQ=255)
                                                                    gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                 >  2:2756886/1‑101 (MQ=255)
                                                                     ggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgc                                >  2:1901291/1‑101 (MQ=255)
                                                                     ggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgc                                <  1:73024/101‑1 (MQ=255)
                                                                      gggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgcg                               <  1:905763/101‑1 (MQ=255)
                                                                        gCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcc                             <  2:4008288/101‑1 (MQ=255)
                                                                         cGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccc                            <  1:719294/101‑1 (MQ=255)
                                                                         cGTGGACCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccc                            <  2:3324356/101‑1 (MQ=255)
                                                                          gTGGATCGAAACTCCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                           <  1:3835897/101‑1 (MQ=255)
                                                                          gTGGATCGAAACCCCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                           <  1:2800999/101‑1 (MQ=255)
                                                                           tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                          >  1:1254118/1‑101 (MQ=255)
                                                                           tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                          >  2:3580776/1‑101 (MQ=255)
                                                                            ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                         <  1:1783097/101‑1 (MQ=255)
                                                                            ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                         <  2:665116/101‑1 (MQ=255)
                                                                            ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                         >  1:2014616/1‑101 (MQ=255)
                                                                            ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                         >  1:2040226/1‑101 (MQ=255)
                                                                            ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                         >  1:154644/1‑101 (MQ=255)
                                                                            ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                         <  1:4304761/101‑1 (MQ=255)
                                                                             gATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACg                        >  1:3946240/1‑101 (MQ=255)
                                                                              aTCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGc                       >  1:815715/1‑101 (MQ=255)
                                                                               tCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGcc                      >  2:2725889/1‑101 (MQ=255)
                                                                                cGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCa                     <  1:4382560/101‑1 (MQ=255)
                                                                                 gAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAg                    >  2:853518/1‑101 (MQ=255)
                                                                                 gAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAg                    <  1:4332479/101‑1 (MQ=255)
                                                                                 gAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAg                    <  1:1338806/101‑1 (MQ=255)
                                                                                    acacCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacg                 >  2:3279746/1‑101 (MQ=255)
                                                                                     cacCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg                <  2:730108/101‑1 (MQ=255)
                                                                                     cacCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg                >  2:840500/1‑101 (MQ=255)
                                                                                      acCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgga               <  2:3710819/101‑1 (MQ=255)
                                                                                       ccGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggac              >  1:987724/1‑101 (MQ=255)
                                                                                        cgc.gCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggacg             <  1:4145097/101‑1 (MQ=255)
                                                                                              aCGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCt        <  2:2836008/101‑1 (MQ=255)
                                                                                                 cgcgAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccc     >  1:1425434/1‑101 (MQ=255)
                                                                                                    gAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccccc  <  1:458742/101‑1 (MQ=255)
                                                                                                     |                                                                                                      
CGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCC  >  NC_014034/1397180‑1397379

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG