Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,282 C→T 3.1% N388N (AAC→TAT RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2820C→T 3.1% 7.4 ‑1.5 ‑0.1191N388N (AAC→AATRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/5):  ref base (91/93):  total (93/98)
Fisher's exact test strand distribution p-value = 2.13e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.38e-01

Only 100 of 208 total aligned reads displayed.

GGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCG  >  NC_014034/1397182‑1397382
                                                                                                     |                                                                                                         
ggggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAAc                                                                                                           >  2:3137701/1‑101 (MQ=255)
 gggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACg                                                                                                          >  2:3753936/1‑101 (MQ=255)
  ggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACgg                                                                                                         <  2:1333082/101‑1 (MQ=255)
    cGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.g                                                                                                      >  2:458698/1‑101 (MQ=255)
    cGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.g                                                                                                      >  2:2052277/1‑101 (MQ=255)
    cGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.g                                                                                                      >  2:4170146/1‑101 (MQ=255)
      tGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTc                                                                                                    >  2:1548726/1‑101 (MQ=255)
       ggATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.a                                                                                                  >  1:771139/1‑101 (MQ=255)
       ggATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.a                                                                                                  >  1:2314321/1‑101 (MQ=255)
       ggATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.a                                                                                                  >  1:1915049/1‑101 (MQ=255)
        gATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.Ag                                                                                                 >  2:470730/1‑101 (MQ=255)
         aTCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGCAACACCGG.GCACGCGCGAACGGC.GTC.AGc                                                                                                >  2:3891448/1‑101 (MQ=255)
          tCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.c                                                                                              <  1:2037716/101‑1 (MQ=255)
           cGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.cc                                                                                             >  1:2729149/1‑101 (MQ=255)
           cGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.cc                                                                                             >  1:1782861/1‑101 (MQ=255)
            gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                                                                            <  1:297238/101‑1 (MQ=255)
            gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                                                                            <  1:602104/101‑1 (MQ=255)
            gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                                                                            >  1:665180/1‑101 (MQ=255)
            gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                                                                            <  1:917770/101‑1 (MQ=255)
            gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                                                                            >  1:2441289/1‑101 (MQ=255)
            gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                                                                            <  2:4313285/101‑1 (MQ=255)
             aaaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcg                                                                                           >  2:3430653/1‑101 (MQ=255)
               aCGTGTCTGCCACGCCGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcgcg                                                                                         <  1:1577406/101‑1 (MQ=255)
                 gtgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGaa                                                                                       >  1:1002314/1‑101 (MQ=255)
                 gtgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGaa                                                                                       >  1:2584528/1‑101 (MQ=255)
                 gtgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGaa                                                                                       >  1:1595819/1‑101 (MQ=255)
                  tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..c                                                                                    >  1:3748118/1‑101 (MQ=255)
                  tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..c                                                                                    >  1:4527640/1‑101 (MQ=255)
                   gtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..Cg                                                                                   >  2:1876408/1‑101 (MQ=255)
                     cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTc                                                                                 <  2:1635918/101‑1 (MQ=255)
                     cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTc                                                                                 >  2:1767154/1‑101 (MQ=255)
                         cACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTc                                                                             <  2:2484602/101‑1 (MQ=255)
                            gacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTcccc                                                                          <  2:4381541/101‑1 (MQ=255)
                              cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTcccccc                                                                        <  2:1916070/101‑1 (MQ=255)
                                   tGCACCAGATCCGCGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCggg                                                                   >  1:723438/1‑101 (MQ=255)
                                   tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCggg                                                                   <  2:4073580/101‑1 (MQ=255)
                                   tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCggg                                                                   <  1:217218/101‑1 (MQ=255)
                                   tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCggg                                                                   <  2:824726/101‑1 (MQ=255)
                                   cgCACCAGCTCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCggg                                                                   <  2:4525893/100‑1 (MQ=255)
                                      aCCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGc                                                                >  1:2517041/1‑101 (MQ=255)
                                       ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..GGTCGCTCCCCCCGCGGGAGCg                                                               >  1:3868310/1‑101 (MQ=255)
                                         ggccctcgcacGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGAC.GGA.CCCGC..C..CGTCGCTCCCCCCGCGGGGGCGTg                                                             <  2:2763590/91‑1 (MQ=2)
                                         ggccctcgcacGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGAC.GGA.CCCGC..C..CGTCGCTCCCCCCGCGGGGGCGTg                                                             <  2:110177/91‑1 (MQ=2)
                                          gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTgg                                                            >  1:503703/1‑101 (MQ=255)
                                            tCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGAt                                                          <  2:1458323/101‑1 (MQ=255)
                                             ccGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATc                                                         <  1:3430860/101‑1 (MQ=255)
                                              cgcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCg                                                        <  2:2908132/101‑1 (MQ=255)
                                                 gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGaaa                                                     <  2:2083607/101‑1 (MQ=255)
                                                  ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCCGT.GCGCGCCATGAATTC.ATCGAGCGCCATCGAG..CGGCGCTCCCCCCGCGGGGGCGTGGCTCGaa                                                      >  2:3690009/1‑101 (MQ=2)
                                                  ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAAc                                                    <  2:2314188/101‑1 (MQ=255)
                                                   cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCCCGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGaaaag                                                   >  2:1084881/1‑99 (MQ=255)
                                                   cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                   >  2:467856/1‑101 (MQ=255)
                                                   cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                   >  1:180432/1‑101 (MQ=255)
                                                    gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACgg                                                  <  1:1182131/101‑1 (MQ=255)
                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                               >  1:3649724/1‑101 (MQ=255)
                                                        gatcgcccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                              >  2:1974551/6‑101 (MQ=255)
                                                        ccccccccCGGGGGGCGTGGATCGAAACACCCC.GCACCTGTCGGCGGC.G...AAC.ACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatcgat                                              <  2:1989096/99‑7 (MQ=0)
                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                              >  1:1058578/1‑101 (MQ=255)
                                                          ccccccGCGGGGGCGTGGATCGAAACCCCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTAc                                            <  1:2655/101‑1 (MQ=255)
                                                            ccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAg                                          >  2:3248165/1‑101 (MQ=255)
                                                               cgcgGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGAC.GGA.CCCGC..C..CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgccc                                       >  2:4457308/1‑91 (MQ=0)
                                                                gcgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                      <  2:2170819/101‑1 (MQ=255)
                                                                 cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                     >  2:3379979/1‑101 (MQ=255)
                                                                 cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                     <  1:3533815/101‑1 (MQ=255)
                                                                 cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                     <  2:2642265/101‑1 (MQ=255)
                                                                  gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                    <  1:3356117/101‑1 (MQ=255)
                                                                  gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                    >  2:2756886/1‑101 (MQ=255)
                                                                       cGTGGACCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccc                               <  2:3324356/101‑1 (MQ=255)
                                                                        gTGGATCGAAACTCCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                              <  1:3835897/101‑1 (MQ=255)
                                                                        gTGGATCGAAACCCCGC.GCCCGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                              <  1:3117436/101‑1 (MQ=255)
                                                                        gTGGATCGAAACCCCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                              <  1:2800999/101‑1 (MQ=255)
                                                                         tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccccc                             >  2:1222747/1‑100 (MQ=255)
                                                                         tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                             >  2:3580776/1‑101 (MQ=255)
                                                                         tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                             >  1:1254118/1‑101 (MQ=255)
                                                                          ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                            >  1:154644/1‑101 (MQ=255)
                                                                          ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                            <  1:4304761/101‑1 (MQ=255)
                                                                          ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                            >  1:2014616/1‑101 (MQ=255)
                                                                          ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                            >  1:4174102/1‑101 (MQ=255)
                                                                          ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                            <  2:665116/101‑1 (MQ=255)
                                                                          ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                            <  1:885534/101‑1 (MQ=255)
                                                                           gATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACg                           >  2:4636233/1‑101 (MQ=255)
                                                                           gATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACg                           >  1:3946240/1‑101 (MQ=255)
                                                                             tCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGcc                         >  2:2725889/1‑101 (MQ=255)
                                                                              cGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCa                        <  1:4382560/101‑1 (MQ=255)
                                                                               gAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAg                       >  2:853518/1‑101 (MQ=255)
                                                                               gAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAg                       <  1:1338806/101‑1 (MQ=255)
                                                                               gAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAg                       <  1:4332479/101‑1 (MQ=255)
                                                                                 aaCACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgac                     <  2:3968184/101‑1 (MQ=255)
                                                                                  acacCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacg                    >  2:3279746/1‑101 (MQ=255)
                                                                                   cacCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg                   <  2:730108/101‑1 (MQ=255)
                                                                                   cacCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg                   >  2:840500/1‑101 (MQ=255)
                                                                                    acCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgga                  <  1:2052398/101‑1 (MQ=255)
                                                                                       gc.gcACGCGCGAACGGC.GTC.CGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCCCGCCCCACGCCAGACGGACGt               >  2:2254652/1‑101 (MQ=255)
                                                                                       gc.gcACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGt               <  1:2561423/101‑1 (MQ=255)
                                                                                        c.gcACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTc              <  1:3294199/101‑1 (MQ=255)
                                                                                          gcACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCg             <  1:3248366/101‑1 (MQ=255)
                                                                                          gcACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCg             <  2:200671/101‑1 (MQ=255)
                                                                                               cgcgAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccc        >  1:1425434/1‑101 (MQ=255)
                                                                                                 cgAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccccc      >  1:4136184/1‑101 (MQ=255)
                                                                                                  gAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccccc     <  1:458742/101‑1 (MQ=255)
                                                                                                     |                                                                                                         
GGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCG  >  NC_014034/1397182‑1397382

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG