Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,287 T→A 3.3% V390D (GTC→GAC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2870T→A 3.3% 9.1 ‑1.5 ‑0.3185V390D (GTC→GAC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/5):  ref base (84/93):  total (86/99)
Fisher's exact test strand distribution p-value = 2.18e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.88e-01

Only 100 of 200 total aligned reads displayed.

GTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1397187‑1397387
                                                                                                      |                                                                                                      
gTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.Gt                                                                                                        >  1:2509317/1‑101 (MQ=255)
 tGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTc                                                                                                       >  2:1548726/1‑101 (MQ=255)
  ggATCGAAACGTGCCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCa                                                                                                      >  2:3689611/1‑101 (MQ=255)
    aTCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGCAACACCGG.GCACGCGCGAACGGC.GTCAGc                                                                                                    >  2:3891448/1‑101 (MQ=255)
    aTCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGc                                                                                                    >  2:3293997/1‑101 (MQ=255)
     tCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGcc                                                                                                   <  2:1506648/101‑1 (MQ=255)
      cGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGccc                                                                                                  >  1:598870/1‑101 (MQ=255)
      cGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGccc                                                                                                  >  1:1782861/1‑101 (MQ=255)
       gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGcccc                                                                                                 >  2:2020094/1‑101 (MQ=255)
       gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGcccc                                                                                                 >  1:2989686/1‑101 (MQ=255)
        aaaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCcg                                                                                                >  2:719221/1‑101 (MQ=255)
        aaaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GCCAGccccc                                                                                                >  1:2404391/1‑100 (MQ=255)
          aCGTGTCTGCCACGCCGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCcgcg                                                                                              <  1:1577406/101‑1 (MQ=255)
          aCGTGTCTGCCACGACGCCCTGCACTAGATACGCGCCGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACACCGC.GCACGCGCGCACCGC.CTCAGCCCcgcg                                                                                              >  2:3257089/1‑101 (MQ=37)
            gtgtCTGCCCCGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGaa                                                                                            <  1:2640068/101‑1 (MQ=255)
            gtgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGaa                                                                                            >  1:1002314/1‑101 (MQ=255)
            gtgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGaa                                                                                            >  1:1595819/1‑101 (MQ=255)
             tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..c                                                                                         >  1:4527640/1‑101 (MQ=255)
             tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..c                                                                                         >  1:3748118/1‑101 (MQ=255)
             tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..c                                                                                         <  2:4314565/101‑1 (MQ=255)
              gtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..Cg                                                                                        >  2:1876408/1‑101 (MQ=255)
              gtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..Cg                                                                                        <  1:2545195/101‑1 (MQ=255)
               tCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGt                                                                                       <  1:636198/101‑1 (MQ=255)
                cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTc                                                                                      <  2:1635918/101‑1 (MQ=255)
                 tGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCg                                                                                     >  2:3247741/1‑101 (MQ=255)
                   ccACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCt                                                                                   >  2:4212284/1‑101 (MQ=255)
                   ccACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCCCt                                                                                   <  1:2715082/101‑1 (MQ=255)
                    cACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTc                                                                                  <  2:2484602/101‑1 (MQ=255)
                       gacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTcccc                                                                               >  2:3355915/1‑101 (MQ=255)
                       gacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTcccc                                                                               <  2:4381541/101‑1 (MQ=255)
                         cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTcccccc                                                                             <  1:2020211/101‑1 (MQ=255)
                         cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTcccccc                                                                             <  2:1916070/101‑1 (MQ=255)
                             cTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCgg                                                                         >  1:3309977/1‑101 (MQ=255)
                              tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCggg                                                                        <  2:4073580/101‑1 (MQ=255)
                              cgCACCAGCTCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCggg                                                                        <  2:4525893/100‑1 (MQ=255)
                                  ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..GGTCGCTCCCCCCGCGGGAGCg                                                                    >  1:3868310/1‑101 (MQ=255)
                                    ggccctcgcacGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGACGGACCCGC..C..CGTCGCTCCCCCCGCGGGGGCGTg                                                                  <  2:2763590/91‑1 (MQ=2)
                                    aGATCCGCGCCGTCGCTCCCCCCGCGGGGGGGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTg                                                                  >  1:4222772/1‑101 (MQ=255)
                                    aGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTg                                                                  >  2:4628669/1‑101 (MQ=255)
                                        ccGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATc                                                              <  1:3430860/101‑1 (MQ=255)
                                         cgcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCg                                                             <  2:2908132/101‑1 (MQ=255)
                                           cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGaa                                                           <  2:641752/101‑1 (MQ=255)
                                            gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGCACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGaaa                                                          <  1:4663260/101‑1 (MQ=255)
                                            gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGaaa                                                          <  2:2083607/101‑1 (MQ=255)
                                            gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGaaa                                                          <  1:225900/101‑1 (MQ=255)
                                             ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAAc                                                         <  2:2314188/101‑1 (MQ=255)
                                             ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAAc                                                         <  2:771065/101‑1 (MQ=255)
                                              cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCCCGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGaaaag                                                        >  2:1084881/1‑99 (MQ=255)
                                              cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                        >  1:180432/1‑101 (MQ=255)
                                               gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACgg                                                       <  1:1182131/101‑1 (MQ=255)
                                                 cGCTCCCCCCGCAGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCt                                                     >  1:2569660/1‑101 (MQ=255)
                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                    >  1:1936383/1‑101 (MQ=255)
                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                    >  1:3649724/1‑101 (MQ=255)
                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                    >  1:2166542/1‑101 (MQ=255)
                                                   gatcgcccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                                   >  2:1974551/6‑101 (MQ=255)
                                                   ccccccccCGGGGGGCGTGGATCGAAACACCCC.GCACCTGTCGGCGGC.G..AACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatcgat                                                   <  2:1989096/99‑7 (MQ=0)
                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                                   >  1:1058578/1‑101 (MQ=255)
                                                          cgcgGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGACGGACCCGC..C..CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgccc                                            >  2:4457308/1‑91 (MQ=0)
                                                           gcgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                           <  2:2170819/101‑1 (MQ=255)
                                                            cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                          <  2:2642265/101‑1 (MQ=255)
                                                            cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                          <  1:3533815/101‑1 (MQ=255)
                                                             gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                         >  2:2756886/1‑101 (MQ=255)
                                                              ggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgc                                        <  1:73024/101‑1 (MQ=255)
                                                               gggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgcg                                       <  1:905763/101‑1 (MQ=255)
                                                                 gCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcc                                     >  2:829068/1‑101 (MQ=255)
                                                                  cGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccc                                    <  1:719294/101‑1 (MQ=255)
                                                                   gTGGATCGAAACTCCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                                   <  1:3835897/101‑1 (MQ=255)
                                                                   gTGGATCGAAACCCCGC.GCCCGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                                   <  1:3117436/101‑1 (MQ=255)
                                                                    tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccccc                                  >  2:1222747/1‑100 (MQ=255)
                                                                    tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                                  <  2:3385972/101‑1 (MQ=255)
                                                                    tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                                  >  1:1254118/1‑101 (MQ=255)
                                                                     ggATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                 >  1:4174102/1‑101 (MQ=255)
                                                                     ggATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                 >  1:2040226/1‑101 (MQ=255)
                                                                     ggATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                 >  1:154644/1‑101 (MQ=255)
                                                                      gATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACg                                >  1:3946240/1‑101 (MQ=255)
                                                                       aTCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGc                               >  1:815715/1‑101 (MQ=255)
                                                                        tCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGcc                              >  2:2725889/1‑101 (MQ=255)
                                                                        tCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGcc                              >  1:2772050/1‑101 (MQ=255)
                                                                         cGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCa                             <  1:4382560/101‑1 (MQ=255)
                                                                         cGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCa                             <  2:4693642/101‑1 (MQ=255)
                                                                          gAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAg                            <  1:1338806/101‑1 (MQ=255)
                                                                          gAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAg                            <  1:4332479/101‑1 (MQ=255)
                                                                            aaCACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgac                          <  2:3968184/101‑1 (MQ=255)
                                                                              cacCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg                        >  2:3499953/1‑101 (MQ=255)
                                                                              cacCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg                        <  1:2915482/101‑1 (MQ=255)
                                                                              cacCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCT.CCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgga                       <  2:842165/101‑1 (MQ=255)
                                                                               acCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgga                       <  2:3710819/101‑1 (MQ=255)
                                                                               acCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgga                       >  2:464400/1‑101 (MQ=255)
                                                                                ccGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggac                      >  1:987724/1‑101 (MQ=255)
                                                                                  gc.gcACGCGCGAACGGC.GTCCGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCCCGCCCCACGCCAGACGGACGt                    >  2:2254652/1‑101 (MQ=255)
                                                                                   c.gcACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTc                   >  2:870618/1‑101 (MQ=255)
                                                                                   c.gcACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTc                   <  1:3294199/101‑1 (MQ=255)
                                                                                     gcACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCg                  <  1:3248366/101‑1 (MQ=255)
                                                                                     gcACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCg                  <  2:200671/101‑1 (MQ=255)
                                                                                          cgcgAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccc             >  1:1425434/1‑101 (MQ=255)
                                                                                            cgAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccccc           >  1:4136184/1‑101 (MQ=255)
                                                                                                cGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcgcg       <  1:1901403/101‑1 (MQ=255)
                                                                                                cGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcgcg       <  2:1360037/101‑1 (MQ=255)
                                                                                                   c.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgggg    <  2:1123752/101‑1 (MQ=255)
                                                                                                     gTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCggggg   >  1:4568282/1‑101 (MQ=255)
                                                                                                      |                                                                                                      
GTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1397187‑1397387

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG