Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,289 A→G 3.3% S391G (AGC→GGA)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2890A→G 3.3% 5.2 ‑1.5 ‑0.1184S391G (AGC→GGC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/5):  ref base (83/93):  total (85/99)
Fisher's exact test strand distribution p-value = 2.19e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.61e-01

Only 100 of 201 total aligned reads displayed.

GGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1397189‑1397389
                                                                                                       |                                                                                                       
ggATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.a                                                                                                         >  1:771139/1‑101 (MQ=255)
ggATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.a                                                                                                         >  1:2314321/1‑101 (MQ=255)
 gATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.Ag                                                                                                        >  2:470730/1‑101 (MQ=255)
    cGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.cc                                                                                                    >  1:1782861/1‑101 (MQ=255)
     gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                                                                                   <  1:602104/101‑1 (MQ=255)
     gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                                                                                   >  1:665180/1‑101 (MQ=255)
     gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                                                                                   <  1:917770/101‑1 (MQ=255)
     gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                                                                                   >  2:2020094/1‑101 (MQ=255)
     gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                                                                                   >  1:2989686/1‑101 (MQ=255)
     gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                                                                                   <  2:4313285/101‑1 (MQ=255)
     gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                                                                                   >  1:2441289/1‑101 (MQ=255)
      aaaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcg                                                                                                  >  2:3430653/1‑101 (MQ=255)
        aCGTGTCTGCCACGCCGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcgcg                                                                                                <  1:1577406/101‑1 (MQ=255)
          gtgtCTGCCCCGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGaa                                                                                              <  1:2640068/101‑1 (MQ=255)
          gtgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGaa                                                                                              >  1:1002314/1‑101 (MQ=255)
          gtgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGaa                                                                                              >  1:1595819/1‑101 (MQ=255)
           tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGCA..c                                                                                           >  2:1055669/1‑101 (MQ=255)
           tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..c                                                                                           >  1:3748118/1‑101 (MQ=255)
            gtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..Cg                                                                                          >  2:1876408/1‑101 (MQ=255)
            gtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..Cg                                                                                          <  1:2545195/101‑1 (MQ=255)
             tCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGt                                                                                         >  2:1606953/1‑101 (MQ=255)
              cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTc                                                                                        >  2:1767154/1‑101 (MQ=255)
               taacACGAAGCCCTGCACCAGATCCGTGCCGTCGCCTCCGCCGCGGAGGCGCTGCTCCCACGACCGC.GCACGCGCGTAGCGC.GGC.AGT.CCCCCGAA..CGCCg                                                                                       >  2:3901043/4‑101 (MQ=255)
               tGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCg                                                                                       >  2:4369188/1‑101 (MQ=255)
               tGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCg                                                                                       >  1:4537357/1‑101 (MQ=255)
                 ccACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCCCt                                                                                     <  1:2715082/101‑1 (MQ=255)
                  cACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTc                                                                                    <  2:2484602/101‑1 (MQ=255)
                     gacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTcccc                                                                                 >  2:1236384/1‑101 (MQ=255)
                      acgCCCTGCCCCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTccccc                                                                                <  2:120558/101‑1 (MQ=255)
                       cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTcccccc                                                                               <  2:1916070/101‑1 (MQ=255)
                       cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTcccccc                                                                               <  1:2020211/101‑1 (MQ=255)
                        gCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCcg                                                                              <  1:4369435/101‑1 (MQ=255)
                            tGCACCAGATCCGCGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCggg                                                                          >  1:723438/1‑101 (MQ=255)
                            tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCggg                                                                          <  2:824726/101‑1 (MQ=255)
                            tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCggg                                                                          <  1:217218/101‑1 (MQ=255)
                                ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..GGTCGCTCCCCCCGCGGGAGCg                                                                      >  1:3868310/1‑101 (MQ=255)
                                ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCg                                                                      <  2:3050192/101‑1 (MQ=255)
                                  ggccctcgcacGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGAC.GGA.CCCGC..C..CGTCGCTCCCCCCGCGGGGGCGTg                                                                    <  2:2763590/91‑1 (MQ=2)
                                  ggccctcgcacGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGAC.GGA.CCCGC..C..CGTCGCTCCCCCCGCGGGGGCGTg                                                                    <  2:110177/91‑1 (MQ=2)
                                   gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTgg                                                                   >  1:503703/1‑101 (MQ=255)
                                   gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTgg                                                                   >  2:4144851/1‑101 (MQ=255)
                                   gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTgg                                                                   >  1:4522397/1‑101 (MQ=255)
                                   gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGCgg                                                                   >  2:3835662/1‑101 (MQ=255)
                                     tCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGAt                                                                 <  2:1458323/101‑1 (MQ=255)
                                      ccGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATc                                                                <  1:3430860/101‑1 (MQ=255)
                                       cgcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCg                                                               <  2:2908132/101‑1 (MQ=255)
                                          gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGaaa                                                            <  2:2083607/101‑1 (MQ=255)
                                           ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCCGT.GCGCGCCATGAATTC.ATCGAGCGCCATCGAG..CGGCGCTCCCCCCGCGGGGGCGTGGCTCGaa                                                             >  2:3690009/1‑101 (MQ=2)
                                           ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAAc                                                           <  2:2314188/101‑1 (MQ=255)
                                            cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                          >  1:180432/1‑101 (MQ=255)
                                            cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                          >  2:467856/1‑101 (MQ=255)
                                             gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACgg                                                         <  1:1182131/101‑1 (MQ=255)
                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGc                                                        >  2:442795/1‑101 (MQ=255)
                                               cGCTCCCCCCGCAGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCt                                                       >  1:2569660/1‑101 (MQ=255)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                      >  1:1936383/1‑101 (MQ=255)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                      >  1:3649724/1‑101 (MQ=255)
                                                 gatcgcccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                                     >  2:1974551/6‑101 (MQ=255)
                                                 ccccccccCGGGGGGCGTGGATCGAAACACCCC.GCACCTGTCGGCGGC.G...AAC.ACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatcgat                                                     <  2:1989096/99‑7 (MQ=0)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                                     >  1:1058578/1‑101 (MQ=255)
                                                  cccccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTa                                                    <  1:4216544/100‑1 (MQ=255)
                                                     ccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAg                                                 >  2:3248165/1‑101 (MQ=255)
                                                         gcgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                             <  2:2170819/101‑1 (MQ=255)
                                                          cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                            >  2:3379979/1‑101 (MQ=255)
                                                          cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                            <  1:3533815/101‑1 (MQ=255)
                                                          cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                            <  2:2642265/101‑1 (MQ=255)
                                                           gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                           <  1:3356117/101‑1 (MQ=255)
                                                           gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                           >  2:2756886/1‑101 (MQ=255)
                                                            ggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgc                                          <  2:4537104/101‑1 (MQ=255)
                                                               gCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcc                                       <  2:4008288/101‑1 (MQ=255)
                                                                cGTGGACCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccc                                      <  2:3324356/101‑1 (MQ=255)
                                                                 gTGGATCGAAACTCCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                                     <  1:3835897/101‑1 (MQ=255)
                                                                 gTGGATCGAAACCCCGC.GCCCGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                                     <  1:3117436/101‑1 (MQ=255)
                                                                  tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                                    >  1:1254118/1‑101 (MQ=255)
                                                                  tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                                    >  2:3580776/1‑101 (MQ=255)
                                                                   ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                   <  2:665116/101‑1 (MQ=255)
                                                                   ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                   >  1:154644/1‑101 (MQ=255)
                                                                   ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                   <  1:885534/101‑1 (MQ=255)
                                                                   ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                   <  1:4304761/101‑1 (MQ=255)
                                                                   ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                   >  1:2040226/1‑101 (MQ=255)
                                                                    gATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACg                                  >  2:4636233/1‑101 (MQ=255)
                                                                    gATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACg                                  >  1:3946240/1‑101 (MQ=255)
                                                                      tCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGcc                                >  2:2725889/1‑101 (MQ=255)
                                                                      tCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGcc                                >  1:2772050/1‑101 (MQ=255)
                                                                        gAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAg                              >  2:853518/1‑101 (MQ=255)
                                                                        gAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAg                              <  1:1338806/101‑1 (MQ=255)
                                                                           acacCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacg                           >  2:3279746/1‑101 (MQ=255)
                                                                            cacCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg                          <  1:2915482/101‑1 (MQ=255)
                                                                            cacCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg                          <  2:730108/101‑1 (MQ=255)
                                                                            cacCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg                          >  2:840500/1‑101 (MQ=255)
                                                                               cgc.gCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggacg                       <  1:4145097/101‑1 (MQ=255)
                                                                                gc.gcACGCGCGAACGGC.GTC.CGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCCCGCCCCACGCCAGACGGACGt                      >  2:2254652/1‑101 (MQ=255)
                                                                                 c.gcACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTc                     <  1:3294199/101‑1 (MQ=255)
                                                                                   gcACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCg                    <  1:3248366/101‑1 (MQ=255)
                                                                                   gcACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCg                    <  2:200671/101‑1 (MQ=255)
                                                                                        cgcgAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccc               >  1:1425434/1‑101 (MQ=255)
                                                                                           gAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccccc            <  1:458742/101‑1 (MQ=255)
                                                                                             aCGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcgc          <  2:4487458/101‑1 (MQ=255)
                                                                                              cGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcgcg         <  1:1901403/101‑1 (MQ=255)
                                                                                                       aGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGt  >  1:2106694/1‑101 (MQ=255)
                                                                                                       aGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAAGCCAGACGGACGTCGCTCCCCCCGCGGGGGCgc  >  1:4111251/1‑100 (MQ=255)
                                                                                                       |                                                                                                       
GGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1397189‑1397389

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG