Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,291 C→A 3.5% S391R (AGC→GGA RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2910C→A 3.5% 7.4 ‑1.5 ‑0.0169S391R (AGC→AGARCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/5):  ref base (76/87):  total (77/92)
Fisher's exact test strand distribution p-value = 2.22e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.96e-01

Only 100 of 197 total aligned reads displayed.

ATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1397191‑1397391
                                                                                                      |                                                                                                      
aTCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGCAACACCGG.GCACGCGCGAACGGC.GTCAGc                                                                                                        >  2:3891448/1‑101 (MQ=255)
aTCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGc                                                                                                        >  1:1330050/1‑101 (MQ=255)
 tCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGcc                                                                                                       <  1:2037716/101‑1 (MQ=255)
  cGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGccc                                                                                                      >  1:598870/1‑101 (MQ=255)
   gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGcccc                                                                                                     >  1:2989686/1‑101 (MQ=255)
   gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGcccc                                                                                                     >  2:2020094/1‑101 (MQ=255)
   gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGcccc                                                                                                     <  2:4313285/101‑1 (MQ=255)
   gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGcccc                                                                                                     >  1:2441289/1‑101 (MQ=255)
   gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGcccc                                                                                                     <  1:917770/101‑1 (MQ=255)
    aaaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCcg                                                                                                    >  2:3430653/1‑101 (MQ=255)
    aaaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCcg                                                                                                    >  2:719221/1‑101 (MQ=255)
        gtgtCTGCCCCGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGaa                                                                                                <  1:2640068/101‑1 (MQ=255)
        gtgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGaa                                                                                                >  1:1002314/1‑101 (MQ=255)
         tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGCA..c                                                                                             >  2:1055669/1‑101 (MQ=255)
         tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..c                                                                                             >  1:4527640/1‑101 (MQ=255)
         tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..c                                                                                             >  1:3748118/1‑101 (MQ=255)
          gtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..Cg                                                                                            <  1:2545195/101‑1 (MQ=255)
          gtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..Cg                                                                                            >  2:1876408/1‑101 (MQ=255)
           tCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGt                                                                                           <  1:636198/101‑1 (MQ=255)
           tCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGt                                                                                           >  2:1606953/1‑101 (MQ=255)
            cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTc                                                                                          >  2:1767154/1‑101 (MQ=255)
             tGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCg                                                                                         >  1:1557235/1‑101 (MQ=255)
             tGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCg                                                                                         >  2:4369188/1‑101 (MQ=255)
              gCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGc                                                                                        <  1:3975146/101‑1 (MQ=255)
               ccACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCCCt                                                                                       <  1:2715082/101‑1 (MQ=255)
                   gacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTcccc                                                                                   >  2:1236384/1‑101 (MQ=255)
                    acgCCCTGCCCCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTccccc                                                                                  <  2:120558/101‑1 (MQ=255)
                     cgCCCTGCCCCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTcccccc                                                                                 <  1:1707887/101‑1 (MQ=255)
                     cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTcccccc                                                                                 <  2:1916070/101‑1 (MQ=255)
                          tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGACCGAAACACCGC.GCACGCGCGCATGGC.GTAAGCCCCGCGAC..CGTCGCTTCCCCCGCggg                                                                            >  2:2275070/1‑101 (MQ=37)
                              ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCg                                                                        <  2:3050192/101‑1 (MQ=255)
                                ggccctcgcacGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGACGGACCCGC..C..CGTCGCTCCCCCCGCGGGGGCGTg                                                                      <  2:2763590/91‑1 (MQ=2)
                                ggccctcgcacGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGACGGACCCGC..C..CGTCGCTCCCCCCGCGGGGGCGTg                                                                      <  2:110177/91‑1 (MQ=2)
                                aGATCCGCGCCGTCGCTCCCCCCGCGGGGGGGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTg                                                                      >  1:4222772/1‑101 (MQ=255)
                                 gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTgg                                                                     >  2:4144851/1‑101 (MQ=255)
                                   tCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGAt                                                                   <  2:1458323/101‑1 (MQ=255)
                                    ccGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATc                                                                  <  1:3430860/101‑1 (MQ=255)
                                     cgcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCg                                                                 <  2:2908132/101‑1 (MQ=255)
                                       cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGGAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGAtggga                                                               >  1:1123838/1‑97 (MQ=255)
                                       cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGaa                                                               <  2:641752/101‑1 (MQ=255)
                                        gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGCACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGaaa                                                              <  1:4663260/101‑1 (MQ=255)
                                        gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGaaa                                                              <  1:225900/101‑1 (MQ=255)
                                        gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGaaa                                                              <  2:2083607/101‑1 (MQ=255)
                                         ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAAc                                                             <  2:771065/101‑1 (MQ=255)
                                          cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                            >  2:467856/1‑101 (MQ=255)
                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGc                                                          >  2:442795/1‑101 (MQ=255)
                                             cGCTCCCCCCGCAGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCt                                                         >  1:2569660/1‑101 (MQ=255)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                        >  1:3649724/1‑101 (MQ=255)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                        >  1:2166542/1‑101 (MQ=255)
                                               gatcgcccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                                       >  2:1974551/6‑101 (MQ=255)
                                               ccccccccCGGGGGGCGTGGATCGAAACACCCC.GCACCTGTCGGCGGC.G..AACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatcgat                                                       <  2:1989096/99‑7 (MQ=0)
                                                tCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTa                                                      >  1:1536504/1‑101 (MQ=255)
                                                   ccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAg                                                   >  2:3248165/1‑101 (MQ=255)
                                                   ccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAg                                                   <  1:1341667/101‑1 (MQ=255)
                                                      cgcgGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGACGGACCCGC..C..CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgcca                                                <  2:2416538/101‑11 (MQ=0)
                                                      cgcgGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGACGGACCCGC..C..CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgcca                                                <  1:1027566/101‑11 (MQ=0)
                                                       gcgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                               <  2:2170819/101‑1 (MQ=255)
                                                        cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                              <  2:2642265/101‑1 (MQ=255)
                                                        cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                              >  1:3868288/1‑101 (MQ=255)
                                                        cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                              >  2:3379979/1‑101 (MQ=255)
                                                        cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                              <  1:3533815/101‑1 (MQ=255)
                                                         gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                             >  2:2756886/1‑101 (MQ=255)
                                                         gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                             >  2:2207947/1‑101 (MQ=255)
                                                         gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                             <  1:3356117/101‑1 (MQ=255)
                                                          ggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgc                                            <  2:4537104/101‑1 (MQ=255)
                                                          ggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgc                                            <  1:73024/101‑1 (MQ=255)
                                                            ggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCgcgc                                          >  1:1202350/1‑101 (MQ=255)
                                                            ggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCgcgc                                          >  1:3944103/1‑101 (MQ=255)
                                                             gCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcc                                         >  2:829068/1‑101 (MQ=255)
                                                             gCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcc                                         >  1:1584606/1‑101 (MQ=255)
                                                              cGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccc                                        <  1:719294/101‑1 (MQ=255)
                                                              cGTGGACCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccc                                        <  2:3324356/101‑1 (MQ=255)
                                                               gTGGATCGAAACCCCGC.GCCCGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                                       <  1:3117436/101‑1 (MQ=255)
                                                                tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                                      >  2:3580776/1‑101 (MQ=255)
                                                                 ggATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                     <  1:885534/101‑1 (MQ=255)
                                                                 ggATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                     >  1:2014616/1‑101 (MQ=255)
                                                                 ggATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                     <  1:1783097/101‑1 (MQ=255)
                                                                 ggATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                     >  1:4174102/1‑101 (MQ=255)
                                                                  gATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACg                                    >  2:4636233/1‑101 (MQ=255)
                                                                    tCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGcc                                  >  1:2772050/1‑101 (MQ=255)
                                                                    tCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGcc                                  >  2:2725889/1‑101 (MQ=255)
                                                                     cGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCa                                 <  1:4382560/101‑1 (MQ=255)
                                                                      gAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAg                                <  1:4332479/101‑1 (MQ=255)
                                                                        aaCACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgac                              <  2:3968184/101‑1 (MQ=255)
                                                                         acacCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacg                             >  2:3279746/1‑101 (MQ=255)
                                                                          cacCGC.GCCCGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg                            <  1:3790313/101‑1 (MQ=255)
                                                                          cacCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg                            <  1:2915482/101‑1 (MQ=255)
                                                                          cacCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCT.CCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgga                           <  2:842165/101‑1 (MQ=255)
                                                                           acCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgga                           <  2:3710819/101‑1 (MQ=255)
                                                                           acCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgga                           <  1:2052398/101‑1 (MQ=255)
                                                                               c.gcACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTc                       >  2:870618/1‑101 (MQ=255)
                                                                               c.gcACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTc                       <  1:3294199/101‑1 (MQ=255)
                                                                                 gcACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCg                      <  1:3248366/101‑1 (MQ=255)
                                                                                 gcACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCg                      <  2:200671/101‑1 (MQ=255)
                                                                                        cgAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccccc               >  1:4136184/1‑101 (MQ=255)
                                                                                           aCGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcgc            <  2:4487458/101‑1 (MQ=255)
                                                                                            cGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcgcg           <  1:1901403/101‑1 (MQ=255)
                                                                                                 gTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCggggg       >  1:4568282/1‑101 (MQ=255)
                                                                                                     gCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTg   <  1:1818564/101‑1 (MQ=255)
                                                                                                      ccccGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTgg  <  2:4071590/101‑1 (MQ=255)
                                                                                                      |                                                                                                      
ATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1397191‑1397391

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG