Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,299 A→C 3.9% N394T (AAC→ACC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2990A→C 3.9% 10.3 ‑1.4 ‑0.5179N394T (AAC→ACC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/6):  ref base (72/100):  total (73/106)
Fisher's exact test strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.51e-01

Only 100 of 201 total aligned reads displayed.

GTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1397199‑1397399
                                                                                                        |                                                                                                      
gtgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGaa                                                                                                        >  1:1002314/1‑101 (MQ=255)
gtgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGaa                                                                                                        >  1:1595819/1‑101 (MQ=255)
 tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGCA..c                                                                                                     >  2:1055669/1‑101 (MQ=255)
 tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..c                                                                                                     <  2:2115297/101‑1 (MQ=255)
 tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..c                                                                                                     >  1:3748118/1‑101 (MQ=255)
  gtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..Cg                                                                                                    <  1:2545195/101‑1 (MQ=255)
   tCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGt                                                                                                   >  2:1606953/1‑101 (MQ=255)
     tGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCg                                                                                                 >  1:1557235/1‑101 (MQ=255)
     tGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCg                                                                                                 >  1:4537357/1‑101 (MQ=255)
     tGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCg                                                                                                 >  2:4369188/1‑101 (MQ=255)
     tGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCg                                                                                                 >  2:3247741/1‑101 (MQ=255)
      gCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGc                                                                                                >  2:2075211/1‑101 (MQ=255)
      gCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGc                                                                                                <  1:3975146/101‑1 (MQ=255)
        cACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTc                                                                                              <  2:2484602/101‑1 (MQ=255)
        cACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTc                                                                                              <  2:2696712/101‑1 (MQ=255)
           gacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTcccc                                                                                           >  2:3355915/1‑101 (MQ=255)
             cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTcccccc                                                                                         <  1:305639/101‑1 (MQ=255)
                 cTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCgg                                                                                     >  1:3309977/1‑101 (MQ=255)
                  tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCggg                                                                                    <  1:217218/101‑1 (MQ=255)
                  tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCggg                                                                                    <  2:824726/101‑1 (MQ=255)
                  tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGACCGAAACACCGC.GCACGCGCGCATGGC.GTA.AGC.CCCGCGAC..CGTCGCTTCCCCCGCggg                                                                                    >  2:2275070/1‑101 (MQ=37)
                  cgCACCAGCTCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCggg                                                                                    <  2:4525893/100‑1 (MQ=255)
                      ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..GGTCGCTCCCCCCGCGGGAGCg                                                                                >  1:3868310/1‑101 (MQ=255)
                        ggccctcgcacGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGAC.GGA.CCCGC..C..CGTCGCTCCCCCCGCGGGGGCGTg                                                                              <  2:110177/91‑1 (MQ=2)
                        aGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTg                                                                              >  2:4628669/1‑101 (MQ=255)
                         gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTgg                                                                             >  1:503703/1‑101 (MQ=255)
                         gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTgg                                                                             >  1:4522397/1‑101 (MQ=255)
                         gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTgg                                                                             >  2:4144851/1‑101 (MQ=255)
                         gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGCgg                                                                             >  2:3835662/1‑101 (MQ=255)
                          aTCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGa                                                                            <  2:2728995/101‑1 (MQ=255)
                           tCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGAt                                                                           <  2:1458323/101‑1 (MQ=255)
                            ccGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATc                                                                          <  1:3430860/101‑1 (MQ=255)
                               cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGaa                                                                       <  2:641752/101‑1 (MQ=255)
                                gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGaaa                                                                      <  1:225900/101‑1 (MQ=255)
                                 ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCCGT.GCGCGCCATGAATTC.ATCGAGCGCCATCGAG..CGGCGCTCCCCCCGCGGGGGCGTGGCTCGaa                                                                       >  2:3690009/1‑101 (MQ=2)
                                   gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACgg                                                                   <  1:1182131/101‑1 (MQ=255)
                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGc                                                                  >  2:442795/1‑101 (MQ=255)
                                     cGCTCCCCCCGCAGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCt                                                                 >  1:2569660/1‑101 (MQ=255)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                                >  1:3649724/1‑101 (MQ=255)
                                       gatcgcccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                                               >  2:1974551/6‑101 (MQ=255)
                                       ccccccccCGGGGGGCGTGGATCGAAACACCCC.GCACCTGTCGGCGGC.G...AAC.ACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatcgat                                                               <  2:1989096/99‑7 (MQ=0)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                                               >  1:1058578/1‑101 (MQ=255)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTa                                                              >  1:1536504/1‑101 (MQ=255)
                                        cccccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTa                                                              <  1:4216544/100‑1 (MQ=255)
                                         ccccccGCGGGGGCGTGGATCGAAACCCCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTAc                                                             <  1:2655/101‑1 (MQ=255)
                                          cccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACa                                                            <  1:3239325/101‑1 (MQ=255)
                                              cgcgGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGAC.GGA.CCCGC..C..CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgcca                                                        <  2:2416538/101‑11 (MQ=0)
                                              cgcgGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGAC.GGA.CCCGC..C..CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgcca                                                        <  1:1027566/101‑11 (MQ=0)
                                               gggggggCGTGGATCGAAACCCCGC.GCACGCGCGAACGGC.GTC.CGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                                       <  1:3285559/99‑1 (MQ=255)
                                                cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                                      <  1:3533815/101‑1 (MQ=255)
                                                 gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                                     >  2:2207947/1‑101 (MQ=255)
                                                  ggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgc                                                    >  2:1901291/1‑101 (MQ=255)
                                                  ggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgc                                                    <  1:73024/101‑1 (MQ=255)
                                                    ggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCgcgc                                                  >  1:3944103/1‑101 (MQ=255)
                                                      cGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccc                                                <  1:719294/101‑1 (MQ=255)
                                                       gTGGATCGAAACTCCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                                               <  1:3835897/101‑1 (MQ=255)
                                                        tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccccc                                              >  2:1222747/1‑100 (MQ=255)
                                                        tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                                              <  2:3385972/101‑1 (MQ=255)
                                                         ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                             <  1:885534/101‑1 (MQ=255)
                                                         ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                             >  1:2040226/1‑101 (MQ=255)
                                                         ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                             >  1:2014616/1‑101 (MQ=255)
                                                            tCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGcc                                          >  1:2772050/1‑101 (MQ=255)
                                                             cGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCa                                         <  2:4693642/101‑1 (MQ=255)
                                                             cGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCa                                         <  1:4382560/101‑1 (MQ=255)
                                                             cGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCa                                         <  2:186084/101‑1 (MQ=255)
                                                              gAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAg                                        <  1:4332479/101‑1 (MQ=255)
                                                              gAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAg                                        <  1:1338806/101‑1 (MQ=255)
                                                              gAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAg                                        >  2:853518/1‑101 (MQ=255)
                                                                aaCACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgac                                      <  2:3968184/101‑1 (MQ=255)
                                                                 acacCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacg                                     >  2:3279746/1‑101 (MQ=255)
                                                                  cacCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg                                    >  2:3499953/1‑101 (MQ=255)
                                                                  cacCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg                                    <  2:730108/101‑1 (MQ=255)
                                                                  cacCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg                                    >  2:840500/1‑101 (MQ=255)
                                                                   acCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgga                                   >  2:464400/1‑101 (MQ=255)
                                                                     cgc.gCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggacg                                 <  1:4145097/101‑1 (MQ=255)
                                                                       c.gcACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTc                               <  2:2757447/101‑1 (MQ=255)
                                                                         gcACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCg                              <  2:200671/101‑1 (MQ=255)
                                                                           aCGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCt                            <  2:2836008/101‑1 (MQ=255)
                                                                              cgcgAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccc                         >  1:1425434/1‑101 (MQ=255)
                                                                                 gAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccccc                      <  1:458742/101‑1 (MQ=255)
                                                                                   aCGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcgc                    <  2:4487458/101‑1 (MQ=255)
                                                                                    cGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcgcg                   <  1:1901403/101‑1 (MQ=255)
                                                                                           c.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGAAGTCGCTCCCCCCGCGGGGGCg             >  2:2942212/1‑101 (MQ=255)
                                                                                             aGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGt            >  1:2106694/1‑101 (MQ=255)
                                                                                             aGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAAGCCAGACGGACGTCGCTCCCCCCGCGGGGGCgc            >  1:4111251/1‑100 (MQ=255)
                                                                                              gC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTg           <  1:1818564/101‑1 (MQ=255)
                                                                                               c.cccGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTgg          <  2:4071590/101‑1 (MQ=255)
                                                                                                 cccGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGa         >  1:2891470/1‑101 (MQ=255)
                                                                                                   cgcgAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCGTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc       <  1:963778/101‑1 (MQ=255)
                                                                                                   cgcgAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc       <  1:1767263/101‑1 (MQ=255)
                                                                                                   cgcgAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc       <  2:1202260/101‑1 (MQ=255)
                                                                                                    gcgAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCg      <  2:1254028/101‑1 (MQ=255)
                                                                                                    gcgAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCg      <  1:1236474/101‑1 (MQ=255)
                                                                                                    gcgAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCg      <  1:1974668/101‑1 (MQ=255)
                                                                                                     cgAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGa     <  1:3392457/101‑1 (MQ=255)
                                                                                                      gAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa    >  1:1795931/1‑101 (MQ=255)
                                                                                                      gAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa    <  2:4222525/101‑1 (MQ=255)
                                                                                                      gAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa    >  2:1655161/1‑101 (MQ=255)
                                                                                                       aa..CGTCGCTCCCCCCGCGGGAGCGTGGATCGCAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa   >  1:4249770/1‑101 (MQ=255)
                                                                                                        a..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc  >  1:2586370/1‑101 (MQ=255)
                                                                                                        |                                                                                                      
GTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1397199‑1397399

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG