Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,318 A→G 4.7% G400G (GGA→GGG RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,3180A→G 4.7% 13.0 ‑2.5 ‑0.7189G400G (GGA→GGGRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/8):  ref base (79/101):  total (80/109)
Fisher's exact test strand distribution p-value = 8.12e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.02e-01

Only 100 of 202 total aligned reads displayed.

ACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAA  >  NC_014034/1397220‑1397418
                                                                                                      |                                                                                                     
 ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCg                                                                                                    <  2:3050192/101‑1 (MQ=255)
   aGATCCGCGCCGTCGCTCCCCCCGCGGGGGGGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTg                                                                                                  >  1:4222772/1‑101 (MQ=255)
   aGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTg                                                                                                  >  2:4628669/1‑101 (MQ=255)
    gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTgg                                                                                                 >  1:4522397/1‑101 (MQ=255)
    gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGCgg                                                                                                 >  2:3835662/1‑101 (MQ=255)
          cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGGAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGAtggga                                                                                           >  1:1123838/1‑97 (MQ=255)
           gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaaa                                                                                          <  2:2083607/101‑1 (MQ=255)
            ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCCGT.GCGCGCCATGAATTC.ATCGAGCGCCATCGAGCGGCGCTCCCCCCGCGGGGGCGTGGCTCGaa                                                                                           >  2:3690009/1‑101 (MQ=2)
            ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAAc                                                                                         <  2:771065/101‑1 (MQ=255)
            ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAAc                                                                                         <  2:2314188/101‑1 (MQ=255)
             cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCCCGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaaaag                                                                                        >  2:1084881/1‑99 (MQ=255)
                 gCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                                                    >  1:1936383/1‑101 (MQ=255)
                  gatcgcccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                                                                   >  2:1974551/6‑101 (MQ=255)
                   tCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.a                                                                                 >  1:1536504/1‑101 (MQ=255)
                    ccccccGCGGGGGCGTGGATCGAAACCCCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.Ac                                                                                <  1:2655/101‑1 (MQ=255)
                        ccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGAC.GGA.CCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgcc                                                                             <  2:1993593/101‑10 (MQ=0)
                         cgcgGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGAC.GGA.CCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgccc                                                                            >  2:4457308/1‑91 (MQ=0)
                         cgcgGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGAC.GGA.CCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgcca                                                                            <  2:2416538/101‑11 (MQ=0)
                          gggggggCGTGGATCGAAACCCCGC.GCACGCGCGAACGGC.GTC.CGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCtt                                                                          <  1:3285559/99‑1 (MQ=255)
                           cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTc                                                                         >  1:3868288/1‑101 (MQ=255)
                            gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTcg                                                                        >  2:2207947/1‑101 (MQ=255)
                            gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTcg                                                                        <  1:3356117/101‑1 (MQ=255)
                             ggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTcgc                                                                       <  1:73024/101‑1 (MQ=255)
                             ggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTcgc                                                                       >  2:1901291/1‑101 (MQ=255)
                              gggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTcgcg                                                                      <  1:905763/101‑1 (MQ=255)
                               ggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCgcgc                                                                     >  1:1202350/1‑101 (MQ=255)
                                gCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGcc                                                                    >  1:1584606/1‑101 (MQ=255)
                                  gTGGATCGAAACCCCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGcccc                                                                  <  1:2800999/101‑1 (MQ=255)
                                   tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGccccc                                                                 >  2:1222747/1‑100 (MQ=255)
                                   tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCa                                                                 <  2:3385972/101‑1 (MQ=255)
                                    ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCAc                                                                <  1:4304761/101‑1 (MQ=255)
                                    ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCAc                                                                >  1:2040226/1‑101 (MQ=255)
                                    ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCAc                                                                <  2:665116/101‑1 (MQ=255)
                                     gATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACg                                                               >  1:3946240/1‑101 (MQ=255)
                                       tCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGcc                                                             >  2:2725889/1‑101 (MQ=255)
                                       tCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACAGCTTT.ACAGCCTTCGCGCCCCACGcc                                                             <  1:3689841/101‑1 (MQ=255)
                                        cGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCa                                                            <  1:4382560/101‑1 (MQ=255)
                                        cGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCa                                                            <  2:4693642/101‑1 (MQ=255)
                                         gAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAg                                                           >  2:853518/1‑101 (MQ=255)
                                         gAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAg                                                           <  1:1338806/101‑1 (MQ=255)
                                           aaCCCCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgac                                                         <  1:3489243/101‑1 (MQ=255)
                                            acacCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacg                                                        >  2:3279746/1‑101 (MQ=255)
                                             cacCGC.GCCCGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacgg                                                       <  1:3790313/101‑1 (MQ=255)
                                             cacCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacgg                                                       >  2:840500/1‑101 (MQ=255)
                                             cacCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacgg                                                       >  2:3499953/1‑101 (MQ=255)
                                              acCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacgga                                                      >  2:464400/1‑101 (MQ=255)
                                               ccGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacggac                                                     >  1:987724/1‑101 (MQ=255)
                                                cgc.gCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacggacg                                                    <  1:4145097/101‑1 (MQ=255)
                                                 gc.gcACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGt                                                   <  1:2561423/101‑1 (MQ=255)
                                                 gc.gcACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGt                                                   <  1:3415900/101‑1 (MQ=255)
                                                  c.gcACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTc                                                  <  2:2757447/101‑1 (MQ=255)
                                                    gcACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCg                                                 <  1:3248366/101‑1 (MQ=255)
                                                      aCGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCt                                               <  2:2836008/101‑1 (MQ=255)
                                                         cgcgAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccc                                            >  1:1425434/1‑101 (MQ=255)
                                                            gAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccccc                                         <  1:458742/101‑1 (MQ=255)
                                                              aCGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcgc                                       <  2:4487458/101‑1 (MQ=255)
                                                                  c.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgggg                                   <  2:1123752/101‑1 (MQ=255)
                                                                      c.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGAAGTCGCTCCCCCCGCGGGGGCg                                >  2:2942212/1‑101 (MQ=255)
                                                                        aGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGt                               >  1:2106694/1‑101 (MQ=255)
                                                                        aGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCAAGCCAGACGGACGTCGCTCCCCCCGCGGGGGCgc                               >  1:4111251/1‑100 (MQ=255)
                                                                         gC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTg                              <  1:1818564/101‑1 (MQ=255)
                                                                          c.cccGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTgg                             <  2:4071590/101‑1 (MQ=255)
                                                                            cccGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGa                            >  1:2891470/1‑101 (MQ=255)
                                                                            cccGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGa                            <  2:503652/101‑1 (MQ=255)
                                                                              cgcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc                          <  1:1767263/101‑1 (MQ=255)
                                                                               gcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCg                         >  1:3935739/1‑101 (MQ=255)
                                                                               gcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCg                         <  1:1236474/101‑1 (MQ=255)
                                                                                cgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                        <  1:3392457/101‑1 (MQ=255)
                                                                                cgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                        <  2:154628/101‑1 (MQ=255)
                                                                                cgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                        <  2:4522144/101‑1 (MQ=255)
                                                                                 gAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                       >  1:1795931/1‑101 (MQ=255)
                                                                                 gAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                       <  2:4222525/101‑1 (MQ=255)
                                                                                 gAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                       >  1:198169/1‑101 (MQ=255)
                                                                                 gAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                       >  2:1655161/1‑101 (MQ=255)
                                                                                 gAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                       <  1:1055748/101‑1 (MQ=255)
                                                                                  aaCGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                      <  1:1004653/101‑1 (MQ=255)
                                                                                  aaCGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                      <  1:2218056/101‑1 (MQ=255)
                                                                                   aCGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                     >  1:2586370/1‑101 (MQ=255)
                                                                                    cGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                    >  2:399795/1‑101 (MQ=255)
                                                                                    cGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                    <  1:840574/101‑1 (MQ=255)
                                                                                     gTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                   >  1:1657000/1‑101 (MQ=255)
                                                                                      tCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActct                  >  2:2732741/1‑101 (MQ=255)
                                                                                       cGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCtt                 >  2:288098/1‑101 (MQ=255)
                                                                                       cGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCtt                 <  1:516969/101‑1 (MQ=255)
                                                                                        gCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTc                <  1:470774/101‑1 (MQ=255)
                                                                                        gCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTc                >  1:3052904/1‑101 (MQ=255)
                                                                                         cTCCCCCTGCGGGGGCGTGGATCGAAACGGCTAT.ACAGCCTTCGCGGCCCACGCCCGACGGTGGACGCTCCCCCCTCGGGGGCGTGCATCGAACCTCTTCa               >  2:4103138/1‑101 (MQ=25)
                                                                                          tCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCaa              >  1:2467883/1‑101 (MQ=255)
                                                                                           ccccccGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAAt             <  1:4212531/101‑1 (MQ=255)
                                                                                             ccccGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATgg           <  2:1350808/101‑1 (MQ=255)
                                                                                              cccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCA.ACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggttc          <  1:3892781/101‑11 (MQ=2)
                                                                                              cccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCA.ACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggttc          <  2:3404748/101‑11 (MQ=2)
                                                                                               ccGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGat         <  2:4410325/101‑1 (MQ=255)
                                                                                                  cgGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATg      <  1:3222735/101‑1 (MQ=255)
                                                                                                    ggAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGc    <  2:1425340/101‑1 (MQ=255)
                                                                                                    ggAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGc    <  1:3580995/101‑1 (MQ=255)
                                                                                                    ggAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGc    <  1:1876520/101‑1 (MQ=255)
                                                                                                     gAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCa   >  2:201719/1‑101 (MQ=255)
                                                                                                      aGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCaa  <  2:2509173/101‑1 (MQ=255)
                                                                                                      aGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCaa  >  2:3344828/1‑101 (MQ=255)
                                                                                                      |                                                                                                     
ACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAA  >  NC_014034/1397220‑1397418

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG